Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE]
Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y.
The Company of Biologists
Published 2018
The Company of Biologists
Published 2018
| Publication Date: |
2018-01-09
|
|---|---|
| Publisher: |
The Company of Biologists
|
| Print ISSN: |
0950-1991
|
| Electronic ISSN: |
1477-9129
|
| Topics: |
Biology
|
| Published by: |
| _version_ | 1836398740550713345 |
|---|---|
| autor | Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y. |
| beschreibung | Zili Zhang, Wei Liu, Lingfeng Zhao, Zhibin Huang, Xiaohui Chen, Ning Ma, Jin Xu, Wenqing Zhang, and Yiyue Zhang T lymphocytes are key cellular components of an acquired immune system and play essential roles in cell-mediated immunity. T cell development occurs in the thymus where 95% of immature thymocytes are eliminated via apoptosis. It is known that mutation of Zeb1 , one of the retinoblastoma 1 (Rb1) target genes, results in a decrease in the number of immature T cells in mice. E2F1, an RB1-interacting protein, has been shown to regulate mature T cell development by interfering with thymocyte apoptosis. However, whether Rb1 regulates thymocyte development in vivo still needs to be further investigated. Here, we use a zebrafish model to investigate the role of Rb1 in T cell development. We show that Rb1-deficient fish exhibit a significant reduction in T cell number during early development that it is attributed to the accelerated apoptosis of immature T cells in a caspase-dependent manner. We further show that E2F1 overexpression could mimic the reduced T lymphocytes phenotype of Rb1 mutants, and E2F1 knockdown could rescue the phenotype in Rb1-deficient mutants. Collectively, our data indicate that the Rb1-E2F1-caspase axis is crucial for protecting immature T cells from apoptosis during early T lymphocyte maturation. |
| citation_standardnr | 6133781 |
| datenlieferant | ipn_articles |
| feed_id | 1748 |
| feed_publisher | The Company of Biologists |
| feed_publisher_url | http://www.biologists.com/ |
| insertion_date | 2018-01-09 |
| journaleissn | 1477-9129 |
| journalissn | 0950-1991 |
| publikationsjahr_anzeige | 2018 |
| publikationsjahr_facette | 2018 |
| publikationsjahr_intervall | 7984:2015-2019 |
| publikationsjahr_sort | 2018 |
| publisher | The Company of Biologists |
| quelle | Development |
| relation | http://dev.biologists.org/cgi/content/short/145/1/dev158139?rss=1 |
| search_space | articles |
| shingle_author_1 | Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y. |
| shingle_author_2 | Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y. |
| shingle_author_3 | Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y. |
| shingle_author_4 | Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y. |
| shingle_catch_all_1 | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] Zili Zhang, Wei Liu, Lingfeng Zhao, Zhibin Huang, Xiaohui Chen, Ning Ma, Jin Xu, Wenqing Zhang, and Yiyue Zhang T lymphocytes are key cellular components of an acquired immune system and play essential roles in cell-mediated immunity. T cell development occurs in the thymus where 95% of immature thymocytes are eliminated via apoptosis. It is known that mutation of Zeb1 , one of the retinoblastoma 1 (Rb1) target genes, results in a decrease in the number of immature T cells in mice. E2F1, an RB1-interacting protein, has been shown to regulate mature T cell development by interfering with thymocyte apoptosis. However, whether Rb1 regulates thymocyte development in vivo still needs to be further investigated. Here, we use a zebrafish model to investigate the role of Rb1 in T cell development. We show that Rb1-deficient fish exhibit a significant reduction in T cell number during early development that it is attributed to the accelerated apoptosis of immature T cells in a caspase-dependent manner. We further show that E2F1 overexpression could mimic the reduced T lymphocytes phenotype of Rb1 mutants, and E2F1 knockdown could rescue the phenotype in Rb1-deficient mutants. Collectively, our data indicate that the Rb1-E2F1-caspase axis is crucial for protecting immature T cells from apoptosis during early T lymphocyte maturation. Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y. The Company of Biologists 0950-1991 09501991 1477-9129 14779129 |
| shingle_catch_all_2 | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] Zili Zhang, Wei Liu, Lingfeng Zhao, Zhibin Huang, Xiaohui Chen, Ning Ma, Jin Xu, Wenqing Zhang, and Yiyue Zhang T lymphocytes are key cellular components of an acquired immune system and play essential roles in cell-mediated immunity. T cell development occurs in the thymus where 95% of immature thymocytes are eliminated via apoptosis. It is known that mutation of Zeb1 , one of the retinoblastoma 1 (Rb1) target genes, results in a decrease in the number of immature T cells in mice. E2F1, an RB1-interacting protein, has been shown to regulate mature T cell development by interfering with thymocyte apoptosis. However, whether Rb1 regulates thymocyte development in vivo still needs to be further investigated. Here, we use a zebrafish model to investigate the role of Rb1 in T cell development. We show that Rb1-deficient fish exhibit a significant reduction in T cell number during early development that it is attributed to the accelerated apoptosis of immature T cells in a caspase-dependent manner. We further show that E2F1 overexpression could mimic the reduced T lymphocytes phenotype of Rb1 mutants, and E2F1 knockdown could rescue the phenotype in Rb1-deficient mutants. Collectively, our data indicate that the Rb1-E2F1-caspase axis is crucial for protecting immature T cells from apoptosis during early T lymphocyte maturation. Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y. The Company of Biologists 0950-1991 09501991 1477-9129 14779129 |
| shingle_catch_all_3 | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] Zili Zhang, Wei Liu, Lingfeng Zhao, Zhibin Huang, Xiaohui Chen, Ning Ma, Jin Xu, Wenqing Zhang, and Yiyue Zhang T lymphocytes are key cellular components of an acquired immune system and play essential roles in cell-mediated immunity. T cell development occurs in the thymus where 95% of immature thymocytes are eliminated via apoptosis. It is known that mutation of Zeb1 , one of the retinoblastoma 1 (Rb1) target genes, results in a decrease in the number of immature T cells in mice. E2F1, an RB1-interacting protein, has been shown to regulate mature T cell development by interfering with thymocyte apoptosis. However, whether Rb1 regulates thymocyte development in vivo still needs to be further investigated. Here, we use a zebrafish model to investigate the role of Rb1 in T cell development. We show that Rb1-deficient fish exhibit a significant reduction in T cell number during early development that it is attributed to the accelerated apoptosis of immature T cells in a caspase-dependent manner. We further show that E2F1 overexpression could mimic the reduced T lymphocytes phenotype of Rb1 mutants, and E2F1 knockdown could rescue the phenotype in Rb1-deficient mutants. Collectively, our data indicate that the Rb1-E2F1-caspase axis is crucial for protecting immature T cells from apoptosis during early T lymphocyte maturation. Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y. The Company of Biologists 0950-1991 09501991 1477-9129 14779129 |
| shingle_catch_all_4 | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] Zili Zhang, Wei Liu, Lingfeng Zhao, Zhibin Huang, Xiaohui Chen, Ning Ma, Jin Xu, Wenqing Zhang, and Yiyue Zhang T lymphocytes are key cellular components of an acquired immune system and play essential roles in cell-mediated immunity. T cell development occurs in the thymus where 95% of immature thymocytes are eliminated via apoptosis. It is known that mutation of Zeb1 , one of the retinoblastoma 1 (Rb1) target genes, results in a decrease in the number of immature T cells in mice. E2F1, an RB1-interacting protein, has been shown to regulate mature T cell development by interfering with thymocyte apoptosis. However, whether Rb1 regulates thymocyte development in vivo still needs to be further investigated. Here, we use a zebrafish model to investigate the role of Rb1 in T cell development. We show that Rb1-deficient fish exhibit a significant reduction in T cell number during early development that it is attributed to the accelerated apoptosis of immature T cells in a caspase-dependent manner. We further show that E2F1 overexpression could mimic the reduced T lymphocytes phenotype of Rb1 mutants, and E2F1 knockdown could rescue the phenotype in Rb1-deficient mutants. Collectively, our data indicate that the Rb1-E2F1-caspase axis is crucial for protecting immature T cells from apoptosis during early T lymphocyte maturation. Zhang, Z., Liu, W., Zhao, L., Huang, Z., Chen, X., Ma, N., Xu, J., Zhang, W., Zhang, Y. The Company of Biologists 0950-1991 09501991 1477-9129 14779129 |
| shingle_title_1 | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] |
| shingle_title_2 | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] |
| shingle_title_3 | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] |
| shingle_title_4 | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] |
| timestamp | 2025-06-30T23:31:53.183Z |
| titel | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] |
| titel_suche | Retinoblastoma 1 protects T cell maturation from premature apoptosis by inhibiting E2F1 [RESEARCH ARTICLE] |
| topic | W |
| uid | ipn_articles_6133781 |