Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster

Publication Date:
2018-01-05
Publisher:
Genetics Society of America (GSA)
Electronic ISSN:
2160-1836
Topics:
Biology
Published by:
http://www.genetics-gsa.org/
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autor Tomaru, M., Ohsako, T., Watanabe, M., Juni, N., Matsubayashi, H., Sato, H., Takahashi, A., Yamamoto, M.-T.
beschreibung In Drosophila , mature sperm are transferred from males to females during copulation, stored in the sperm storage organs of females, and then utilized for fertilization. Here, we report a gene named sheepish ( shps ) of Drosophila melanogaster that is essential for sperm storage in females. shps mutant males, although producing morphologically normal and motile sperm that are effectively transferred to females, produce very few offspring. Direct counts of sperm indicated that the primary defect was correlated to failure of shps sperm to migrate into the female sperm storage organs. Increased sperm motion parameters were seen in the control after transfer to females, whereas sperm from shps males have characteristics of the motion parameters different from the control. The few sperm that occasionally entered the female sperm storage organs showed no obvious defects in fertilization and early embryo development. The female postmating responses after copulation with shps males appeared normal, at least with respect to conformational changes of uterus, mating plug formation, and female remating rates. The shps gene encodes a protein with homology to amine oxidases, including as observed in mammals, with a transmembrane region at the C-terminal end. The shps mutation was characterized by a nonsense replacement in the third exon of CG13611 , and shps was rescued by transformants of the wild-type copy of CG13611 . Thus, shps may define a new class of gene responsible for sperm storage.
citation_standardnr 6130015
datenlieferant ipn_articles
feed_id 169615
feed_publisher Genetics Society of America (GSA)
feed_publisher_url http://www.genetics-gsa.org/
insertion_date 2018-01-05
journaleissn 2160-1836
publikationsjahr_anzeige 2018
publikationsjahr_facette 2018
publikationsjahr_intervall 7984:2015-2019
publikationsjahr_sort 2018
publisher Genetics Society of America (GSA)
quelle G3: Genes, Genomes, Genetics
relation http://www.g3journal.org/cgi/content/short/8/1/149?rss=1
search_space articles
shingle_author_1 Tomaru, M., Ohsako, T., Watanabe, M., Juni, N., Matsubayashi, H., Sato, H., Takahashi, A., Yamamoto, M.-T.
shingle_author_2 Tomaru, M., Ohsako, T., Watanabe, M., Juni, N., Matsubayashi, H., Sato, H., Takahashi, A., Yamamoto, M.-T.
shingle_author_3 Tomaru, M., Ohsako, T., Watanabe, M., Juni, N., Matsubayashi, H., Sato, H., Takahashi, A., Yamamoto, M.-T.
shingle_author_4 Tomaru, M., Ohsako, T., Watanabe, M., Juni, N., Matsubayashi, H., Sato, H., Takahashi, A., Yamamoto, M.-T.
shingle_catch_all_1 Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
In Drosophila , mature sperm are transferred from males to females during copulation, stored in the sperm storage organs of females, and then utilized for fertilization. Here, we report a gene named sheepish ( shps ) of Drosophila melanogaster that is essential for sperm storage in females. shps mutant males, although producing morphologically normal and motile sperm that are effectively transferred to females, produce very few offspring. Direct counts of sperm indicated that the primary defect was correlated to failure of shps sperm to migrate into the female sperm storage organs. Increased sperm motion parameters were seen in the control after transfer to females, whereas sperm from shps males have characteristics of the motion parameters different from the control. The few sperm that occasionally entered the female sperm storage organs showed no obvious defects in fertilization and early embryo development. The female postmating responses after copulation with shps males appeared normal, at least with respect to conformational changes of uterus, mating plug formation, and female remating rates. The shps gene encodes a protein with homology to amine oxidases, including as observed in mammals, with a transmembrane region at the C-terminal end. The shps mutation was characterized by a nonsense replacement in the third exon of CG13611 , and shps was rescued by transformants of the wild-type copy of CG13611 . Thus, shps may define a new class of gene responsible for sperm storage.
Tomaru, M., Ohsako, T., Watanabe, M., Juni, N., Matsubayashi, H., Sato, H., Takahashi, A., Yamamoto, M.-T.
Genetics Society of America (GSA)
2160-1836
21601836
shingle_catch_all_2 Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
In Drosophila , mature sperm are transferred from males to females during copulation, stored in the sperm storage organs of females, and then utilized for fertilization. Here, we report a gene named sheepish ( shps ) of Drosophila melanogaster that is essential for sperm storage in females. shps mutant males, although producing morphologically normal and motile sperm that are effectively transferred to females, produce very few offspring. Direct counts of sperm indicated that the primary defect was correlated to failure of shps sperm to migrate into the female sperm storage organs. Increased sperm motion parameters were seen in the control after transfer to females, whereas sperm from shps males have characteristics of the motion parameters different from the control. The few sperm that occasionally entered the female sperm storage organs showed no obvious defects in fertilization and early embryo development. The female postmating responses after copulation with shps males appeared normal, at least with respect to conformational changes of uterus, mating plug formation, and female remating rates. The shps gene encodes a protein with homology to amine oxidases, including as observed in mammals, with a transmembrane region at the C-terminal end. The shps mutation was characterized by a nonsense replacement in the third exon of CG13611 , and shps was rescued by transformants of the wild-type copy of CG13611 . Thus, shps may define a new class of gene responsible for sperm storage.
Tomaru, M., Ohsako, T., Watanabe, M., Juni, N., Matsubayashi, H., Sato, H., Takahashi, A., Yamamoto, M.-T.
Genetics Society of America (GSA)
2160-1836
21601836
shingle_catch_all_3 Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
In Drosophila , mature sperm are transferred from males to females during copulation, stored in the sperm storage organs of females, and then utilized for fertilization. Here, we report a gene named sheepish ( shps ) of Drosophila melanogaster that is essential for sperm storage in females. shps mutant males, although producing morphologically normal and motile sperm that are effectively transferred to females, produce very few offspring. Direct counts of sperm indicated that the primary defect was correlated to failure of shps sperm to migrate into the female sperm storage organs. Increased sperm motion parameters were seen in the control after transfer to females, whereas sperm from shps males have characteristics of the motion parameters different from the control. The few sperm that occasionally entered the female sperm storage organs showed no obvious defects in fertilization and early embryo development. The female postmating responses after copulation with shps males appeared normal, at least with respect to conformational changes of uterus, mating plug formation, and female remating rates. The shps gene encodes a protein with homology to amine oxidases, including as observed in mammals, with a transmembrane region at the C-terminal end. The shps mutation was characterized by a nonsense replacement in the third exon of CG13611 , and shps was rescued by transformants of the wild-type copy of CG13611 . Thus, shps may define a new class of gene responsible for sperm storage.
Tomaru, M., Ohsako, T., Watanabe, M., Juni, N., Matsubayashi, H., Sato, H., Takahashi, A., Yamamoto, M.-T.
Genetics Society of America (GSA)
2160-1836
21601836
shingle_catch_all_4 Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
In Drosophila , mature sperm are transferred from males to females during copulation, stored in the sperm storage organs of females, and then utilized for fertilization. Here, we report a gene named sheepish ( shps ) of Drosophila melanogaster that is essential for sperm storage in females. shps mutant males, although producing morphologically normal and motile sperm that are effectively transferred to females, produce very few offspring. Direct counts of sperm indicated that the primary defect was correlated to failure of shps sperm to migrate into the female sperm storage organs. Increased sperm motion parameters were seen in the control after transfer to females, whereas sperm from shps males have characteristics of the motion parameters different from the control. The few sperm that occasionally entered the female sperm storage organs showed no obvious defects in fertilization and early embryo development. The female postmating responses after copulation with shps males appeared normal, at least with respect to conformational changes of uterus, mating plug formation, and female remating rates. The shps gene encodes a protein with homology to amine oxidases, including as observed in mammals, with a transmembrane region at the C-terminal end. The shps mutation was characterized by a nonsense replacement in the third exon of CG13611 , and shps was rescued by transformants of the wild-type copy of CG13611 . Thus, shps may define a new class of gene responsible for sperm storage.
Tomaru, M., Ohsako, T., Watanabe, M., Juni, N., Matsubayashi, H., Sato, H., Takahashi, A., Yamamoto, M.-T.
Genetics Society of America (GSA)
2160-1836
21601836
shingle_title_1 Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
shingle_title_2 Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
shingle_title_3 Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
shingle_title_4 Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
timestamp 2025-06-30T23:31:46.217Z
titel Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
titel_suche Severe Fertility Effects of sheepish Sperm Caused by Failure To Enter Female Sperm Storage Organs in Drosophila melanogaster
topic W
uid ipn_articles_6130015