Search Results - (Author, Cooperation:Y. Tomita)
-
1Latest Papers from Table of Contents or Articles in PressNakajima, K., Zhou, Y., Tomita, A., Hirade, Y., Gurumurthy, C. B., Nakada, S.
Cold Spring Harbor Laboratory Press
Published 2018Staff ViewPublication Date: 2018-02-09Publisher: Cold Spring Harbor Laboratory PressElectronic ISSN: 1549-5469Topics: BiologyMedicinePublished by: -
2Latest Papers from Table of Contents or Articles in PressT. Watanabe ; M. Kiso ; S. Fukuyama ; N. Nakajima ; M. Imai ; S. Yamada ; S. Murakami ; S. Yamayoshi ; K. Iwatsuki-Horimoto ; Y. Sakoda ; E. Takashita ; R. McBride ; T. Noda ; M. Hatta ; H. Imai ; D. Zhao ; N. Kishida ; M. Shirakura ; R. P. de Vries ; S. Shichinohe ; M. Okamatsu ; T. Tamura ; Y. Tomita ; N. Fujimoto ; K. Goto ; H. Katsura ; E. Kawakami ; I. Ishikawa ; S. Watanabe ; M. Ito ; Y. Sakai-Tagawa ; Y. Sugita ; R. Uraki ; R. Yamaji ; A. J. Eisfeld ; G. Zhong ; S. Fan ; J. Ping ; E. A. Maher ; A. Hanson ; Y. Uchida ; T. Saito ; M. Ozawa ; G. Neumann ; H. Kida ; T. Odagiri ; J. C. Paulson ; H. Hasegawa ; M. Tashiro ; Y. Kawaoka
Nature Publishing Group (NPG)
Published 2013Staff ViewPublication Date: 2013-07-12Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Antiviral Agents/pharmacology ; Cells, Cultured ; Chickens/virology ; DNA-Directed RNA Polymerases/antagonists & inhibitors ; Dogs ; Enzyme Inhibitors/pharmacology ; Female ; Ferrets/virology ; Humans ; Influenza A Virus, H1N1 Subtype/drug effects/enzymology ; *Influenza A virus/chemistry/drug effects/isolation & purification/pathogenicity ; Influenza, Human/drug therapy/*virology ; Macaca fascicularis/virology ; Madin Darby Canine Kidney Cells ; Male ; Mice ; Mice, Inbred BALB C ; Models, Molecular ; Monkey Diseases/pathology/virology ; Neuraminidase/antagonists & inhibitors ; Orthomyxoviridae Infections/pathology/transmission/*virology ; Quail/virology ; Swine/virology ; Swine, Miniature/virology ; *Virus Replication/drug effectsPublished by: -
3Articles: DFG German National LicensesStaff View
ISSN: 1089-7550Source: AIP Digital ArchiveTopics: PhysicsNotes: Initial behavior and the subsequent motion of a bubble in liquid nitrogen are investigated experimentally using high-speed photography. A bubble is generated by focusing a pulsed ruby laser into liquid nitrogen at 78.0 K, changing the ambient pressures up to 253.2 kPa which corresponds to the applied pressure (or overpressure), Δp, being 147.1 kPa. When the energy level of the laser beam at the focus exceeds an irradiance threshold, for instance 5.4×1011 W/cm2 for Δp=4.9 kPa, the optical breakdown occurs in the liquid nitrogen, followed by a series of high-speed phenomena such as plasma formation, shock wave emission, and vapor bubble generation. It is found that during a very short period after the plasma formation a bubble grows nonspherically reflecting from the plasma shape, but the bubble volume itself varies with time in the same way for all cases performed in the present experiment. The liquid inertia is a dominant factor affecting the bubble growth, while the thermal effect becomes remarkable during the bubble collapse, resulting in the retardation of the bubble motion. The characteristic behavior of a laser-induced cavitation bubble in liquid nitrogen is significantly influenced by the phase change of vapor at the bubble surface as well as by the vapor pressure inside the bubble. Immediately after the bubble rebound, instabilities are amplified over the bubble surface similar to those caused in the water case. © 2000 American Institute of Physics.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 1077-3118Source: AIP Digital ArchiveTopics: PhysicsNotes: Two bubbles are simultaneously generated in water by focusing a Q-switched ruby laser. The dynamic behavior of the induced bubbles is investigated by means of high-speed photography. Consequently it is found that the bubble–bubble interaction is significantly influenced not only by the relative size of bubbles but also by the mutual distance between them. When two bubbles are axisymmetrically produced one behind the other near a rigid wall, we can observe a very interesting phenomenon, bubble splitting followed by bubble pinching.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1468-2494Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: The mechanism of hair growth has been clarifying itself by the improvement in the culture techniques for hair follicle cells. Recently stem cells inducing the anagen phase in the hair follicle cycle have been discovered in the bulge region of the outer root sheath (ORS). To find growth-promoting agents for the ORS cells, we evaluated the effect of various botanical extracts on the growth of cultured human hair follicles. We found that Laminaria angustata extract increased the ORS cell growth. Further, hair-growth in the shaved skin of C3H mice was also promoted by the topical application of the extract.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesTomita, Y. ; Kodama, T. ; Shima, A.
Woodbury, NY : American Institute of Physics (AIP)
Published 1991Staff ViewISSN: 1077-3118Source: AIP Digital ArchiveTopics: PhysicsNotes: An experimental investigation was made of the motion of a cavitation bubble in the vicinity of a free surface in order to study an induced secondary cavitation during the bubble rebound. A bubble was produced by focusing a ruby laser into water, and its subsequent behavior was observed with a high-speed camera. The deformable nature of both a bubble and a free surface becomes significant as the mutual distance between them is decreased. Immediately after bubble rebound, a secondary cavitation occurs at around zero dynamic pressure region which is developed in water between a rising free surface and a collapsing bubble, due to the local pressure reduction mainly caused by the interaction of expansion waves originated from the surface.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesTakatsu, Y. ; Ohishi, K. ; Tomita, Y. ; Hayashi, M. ; Nakajima, M. ; Akutsu, K.
Oxford, UK : Blackwell Publishing Ltd
Published 2000Staff ViewISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: An efficient incubation and inoculation system for white rust was established using plantlets of chrysanthemum growing in vitro. The internal conditions of a culture vessel (plant box) were maintained at a humidity of 90-100% and an optimum temperature of 20-25°C, which are suitable conditions to germinate teliospores and basidiospores of the pathogen. Telia were maintained continuously on plants in the plant box and were used as an inoculum for infection experiments throughout the year, allowing differences in susceptibility to white rust among chrysanthemum cultivars to be detected. Susceptibility to white rust in the plant-box evaluation showed a good correlation with the rating of sporulation on plants grown in a greenhouse. The method described here is a simple, space-saving inoculation system to evaluate the susceptibility of chrysanthemums to white rust.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesShimizu, I. ; Tomita, Y. ; Iwai, T. ; Kajiwara, T. ; Okano, S. ; Sueishi, K. ; Nomoto, K. ; Yasui, H.
Oxford, UK; Malden, USA : Blackwell Science Ltd
Published 2005Staff ViewISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: In the present study, we have elucidated the efficacy of two cyclophosphamide (CP)-induced tolerance protocols for the induction of B-cell tolerance against Galα1-3Galβ1-4GlcNAc (αGal) antigens. α1,3-galactosyltransferase-deficient (GalT–/–; H-2b/d) mice received with 1 × 108 AKR (αGal+/+ H-2k) spleen cells (SC) followed by 200 mg/kg CP, or alternatively followed by 200 mg/kg CP, 30 mg/kg Busulfan (BU) and 1 × 108 T-cell-depleted AKR bone marrow cells (BMC). The generation of both anti-αGal and anti-donor antibodies were completely suppressed, but normal antibody production against third party antigens was observed after BALB/c skin grafting in both groups of GalT–/– mice. In GalT–/– mice, treated with SC and CP, mixed chimerism was not observed. Cellular rejection was observed in grafted donor AKR hearts with an absence of humoral rejection, whereas humoral rejection was observed in untreated GalT–/– mice. On the other hand, long-term mixed chimerism and permanent acceptance of donor AKR skin graft and heart graft were achieved in GalT–/– mice treated with SC, CP, BU and BMC. These results demonstrate the efficacy of classical drug-induced tolerance in the induction of B-cell tolerance against αGal antigens. However, induction of stable mixed chimerism was required for the suppression of cellular rejection.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesHASHIMOTO, S. ; GON, Y. ; HAYASHI, S. ; TOMITA, Y. ; YODOI, J. ; HORIE, T.
Oxford, U.K. and Cambridge, USA : Blackwell Science Ltd
Published 1997Staff ViewISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: The authors examined the regulatory effects of tumour necrosis factor-α (TNF-α) on granulocyte macrophage colony stimulating factor (GM-CSF)-, interleukin-3 (IL-3)- or macrophage colony stimulating factor (M-CSF)-induced gene expression of the low affinity receptor for IgE (Fc ε RII) on human monocytes and GM-CSF-, IL-3- or M-CSF-induced soluble Fc ε RII (sFc ε RII) release from monocytes. The expression of GM-CSF-, IL-3- or M-CSF-induced Fc ε RII on the surface of monocytes was reduced by TNF-α. The present analysis was designed to examine whether or not TNF-α could suppress GM-CSF-, IL-3- or M-CSF-induced Fc ε RII messenger RNA (mRNA) expression and enhance the release of sFc ε RII induced by these cytokines. The addition of TNF-α to monocyte cultures with GM-CSF, IL-3 or M-CSF significantly reduced Fc ε RII expression on the surface of monocytes and significantly increased sFc ε RII release from monocytes. These results suggest that TNF-α-dependent reduction of GM-CSF-, IL-3- or M-CSF-induced Fc ε RII expression on the surface of monocytes resulted, at least in part, from the suppression of Fc ε RII mRNA and the enhancement of sFc ε RII release.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 0167-4781Keywords: DOPAchrome tautomerase ; Melanin ; TRP-2 ; cDNASource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesKATO, T. ; SUETAKE, T. ; SUGIYAMA, Y. ; TANITA, Y. ; KUMASAKA, K. ; TAKEMATSU, H. ; TOMITA, Y. ; TAGAMI, H.
Oxford, UK : Blackwell Publishing Ltd
Published 1993Staff ViewISSN: 1365-2230Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: While the incidence of malignant melanoma is much lower in Japanese than in Caucasians, the commonest site of melanoma in Japanese has been reported to be the acral regions of the limbs. The survival rate for acral and nodular melanoma observed at the Department of Dermatology, Tohoku University Hospital in Sendai, Japan from 1969 to 1990 was reviewed. Among 150 melanoma patients 125 (83%) and 17(11%) had primary cutaneous melanoma and mucous membrane melanomas, respectively. Frequent sites for cutaneous melanomas were the sole (31%) and subungual regions (15%). Comparison of the stages of plantar melanoma at diagnosis showed that the proportion of stages III and IV decreased after 1980 with a corresponding increase in those with a tumour thickness of less than 4 mm (stage II). Concurrently, the prognosis of plantar melanoma has improved; the 5-year survival rate in each of the three periods 1969–75, 1976–80 and 1981–85 was 21, 70 and 90%, respectively. This was also the case with subungual melanoma. Such improvements in the prognosis are thought to be mainly due to early detection through the growing public awareness of this life-threatening disease.By contrast cases of nodular melanoma increased sharply after 1980. Among these, the high proportion of patients in advanced stages (stages III and IV) remained static even after 1980, with a resultant low 5-year survival rate in the above mentioned periods of 33, 38 and 18%, respectively.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesMAEDA, K. ; TOMITA, Y. ; FUKUDA, M. ; TAGAMI, H.
Oxford, UK : Blackwell Publishing Ltd
Published 1992Staff ViewISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Staurosporine, a protein kinase (PK) inhibitor, phorbol-12-myristate-13-acetate (PMA), a PKC activator and A23187 calcium ionophore were added to human melanocyte cultures with or without dibutyryl cyclic AMP (dbcAMP). After 2 days' incubation, changes in various melanogenic factors were examined such as tyrosinase activity and the amount of tyrosinase-related protein (TRP) as well as the morphology of the melanocytes, dbcAMP stimulated all the melanogenic factors. Staurosporine increased tyrosinase activity and amount of TRP and caused morphological changes with the formation of numerous dendrites, regardless of the presence of dbcAMP. In contrast, PMA did not significantly affect tyrosinase activity, TRP content or dendrite formation, with or without dbcAMP. The effects of staurosporine on tyrosinase activity and TRP content were completely inhibited by PMA, but PMA did not significantly affect the staurosporine-induced morphological changes. A23187 inhibited both tyrosinase activity and TRP content, regardless of the presence of dbcAMP, but did not affect the morphology of melanocytes. These findings suggest that tyrosinase activity and TRP content are regulated by adenylate cyclase and Ca2+ and partly by PKC, while the morphological features of melanocytes are affected by intracellular cAMP accumulation and by the inhibition of PKC.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: By continuous infusion of angiotensin II, a marked increase in blood flow can be induced in tumour tissues, with no increase in normal tissues. This functional characteristic of tumour blood vessels, i.e., lack of autoregulation, has been utilized as an aid to chemotherapy by selectively enhancing drug delivery to tumour tissues. We describe our results in seven patients with malignant melanoma.Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesSuzuki, T. ; Inagaki, K. ; Fukai, K. ; Obana, A. ; Lee, S-T. ; Tomita, Y.
Oxford, UK : Blackwell Science Ltd
Published 2005Staff ViewISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesHuman papillomaviruses of the mucosal type are present in some cases of extragenital Bowen's diseaseZheng, S. ; Adachi, A. ; Shimizu, M. ; Shibata, S-I. ; Yasue, S. ; Sakakibara, A. ; Sugiura, M. ; Nagasaka, T. ; Tomita, Y.
Oxford, UK : Blackwell Science Ltd
Published 2005Staff ViewISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background Bowen's disease in the genital area is generally considered to be caused by mucosal high-risk human papillomaviruses (HPVs). However, the detection rate and spectrum of HPVs in extragenital Bowen's disease are various and it is not clear to what extent HPV is involved in its pathogenesis.Objectives To assess the degree of association of HPV in extragenital cases by examining detection rates, types, quantities and localization of HPV.Methods A polymerase chain reaction (PCR) approach that we had previously established, which can give sensitive detection of a broad range of HPVs from cutaneous [including epidermodysplasia verruciformis-related HPVs (EV-HPVs)] to mucosal HPVs, was applied to samples from 41 patients with extragenital Bowen's disease and normal skin samples from 48 individuals. Semiquantitative L1-PCR and tyramide-based in situ hybridization (ISH) were also employed for positive cases.Results HPVs belonging to the mucosal high-risk group were detected in three patients with Bowen's disease (7%; two HPV 16 and one HPV 33), with 101−103 copy equivalents per diploid amount of cellular DNA. They were distributed among most nuclei of tumour cells but in none of the cells of adjacent normal skin. HPVs belonging to the cutaneous group were detected in two patients (5%; HPV 27 and HPV 76) at 10−2−10−3 copy equivalents, the same level as in a normal skin specimen positive for type 23 EV-HPV. No positive signals were observed by ISH.Conclusions HPVs belonging to the mucosal high-risk group may participate in the development of extragenital Bowen's disease.Type of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background Human papillomaviruses (HPVs) parasitize human epithelium, but it is not clear where they reside when they do not cause apparent infection. Hair follicles are important candidates as reservoirs.Objectives A patient reported previously by us as having perianal warts caused mainly by HPV 56, demonstrated hair follicles in her genital area which bulged a little from the surface and appeared somewhat enlarged. We therefore examined whether DNA of HPV 56, a member of the mucous high-risk group, might be detectable in these structures.Methods We obtained plucked hairs and performed an examination by polymerase chain reaction and subsequent reverse-phase dot blot hybridization (PCR-RDBH) and in situ hybridization (ISH).Results Strong positive signals were obtained not only with PCR-RDBH but also with ISH.Conclusions Hair follicles in the genital area might serve as reservoirs for HPVs belonging to the mucous high-risk group.Type of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 0005-2760Keywords: (Rat) ; Cultured hepatocyte ; Hormonal induction ; Lipogenic enzyme ; Pyruvate stimulationSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesStaff View
ISSN: 0005-2736Keywords: (Rat kidney) ; Basolateral membrane ; Brush-border membrane ; Chemical modification ; Organic cation transport ; Substrate recognitionSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesStaff View
ISSN: 0006-291XKeywords: [abr] EGF; epidermal growth factor ; [abr] HGF; hepatocyte growth factor ; [abr] PBS; phosphate buffered saline ; [abr] PDGI; platelet derived growth inhibitor ; [abr] TGF; transforming growth factorSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: