Search Results - (Author, Cooperation:Y. Takagi)

2018-05-18

Nature Publishing Group (NPG)

2045-2322

Natural Sciences in General

2011-07-05

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Binding Sites ; Crystallography, X-Ray ; Mediator Complex/*chemistry/*metabolism ; Models, Molecular ; Phosphorylation ; Protein Structure, Tertiary ; Protein Subunits/chemistry/metabolism ; RNA Polymerase II/chemistry/metabolism ; Saccharomyces cerevisiae/*chemistry/enzymology ; Structure-Activity Relationship ; Transcription Factor TFIIH/chemistry/metabolism

0006-291X

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

2018-04-27

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Geosciences

Computer Science

Medicine

Natural Sciences in General

Physics

Biochemistry, Molecular Biology

2018-12-07

American Society of Hematology (ASH)

0006-4971

1528-0020

Biology

Medicine

Hematopoiesis and Stem Cells, Myeloid Neoplasia

1089-7550

AIP Digital Archive

Physics

We investigated the formation process of cross-hatch patterns (CHPs) and the lattice relaxation process in the growth of an (InAs)1(GaAs)4 strained short-period superlattice (SSPS) and an In0.2Ga0.8As alloy layer on GaAs(100) substrates. By using x-ray diffraction and cross-sectional transmission electron microscopy, it was found that the lattice relaxation in the (InAs)1(GaAs)4SSPS proceeded as fast as that in the In0.2Ga0.8As alloy layer. The surfaces of the grown layers showed CHPs, and the surface roughness increased by means of the evolution of the CHPs. The surface roughness of the SSPSs was larger than that of the alloy layers, whereas no apparent difference was observed in the lattice relaxation process between the SSPSs and the alloy layers. Additionally, the height of surface ridges parallel to the [01¯1] direction was higher than that parallel to the [011] direction. We observed the distribution of highly strained InAs and GaAs islands on the surfaces of strained InGaAs layers by using an atomic force microscope. As a result, it was clarified that the InAs islands were accumulated on top of the surface ridges, whereas the GaAs islands were distributed uniformly on the surface of strained InGaAs layer. It was considered that the nonuniform incorporation of In atoms during growth of layers contributes mainly to the evolution of CHPs in the InGaAs-on-GaAs heteroepitaxy. We propose a developmental model of CHPs based on the surface diffusion of the In atoms. In this model, the asymmetry of CHPs was well understood by anisotropy in surface diffusion length of In atoms. Additionally, it was clarified that the evolution of CHPs in the growth of InGaAs at a high temperature can be suppressed by growing a fully relaxed InGaAs layer at a low temperature before the high-temperature growth. © 1999 American Institute of Physics.

Electronic Resource

1089-7550

AIP Digital Archive

Physics

(Zn1−yMgy)1−xAlxO powders were synthesized by the polymerized complex method and then consolidated by spark plasma sintering apparatus. The microscopic structure and thermoelectric properties were examined comparing with the experimental results of the samples prepared by the conventional solid-state reaction method. A small amount of ZnAl2O4 spinel phase as the second phase was observed in the sintered samples with x≥0.02 by x-ray diffraction and a scanning electron microscope. The grain size of the samples prepared by the polymerized complex method is much smaller than that of the samples prepared by the conventional solid-state reaction method. The absolute values of the Seebeck coefficient and electrical resistivity decrease with increasing x up to about x=0.01, but above x=0.01 they are almost independent of x. This result indicates that the solubility limit of Al in Zn1−xAlxO is about x=0.01, which is also confirmed by 27Al nuclear magnetic resonance spectroscopy. At a fixed composition of x, the absolute values of the Seebeck coefficient and electrical resistivity for the samples prepared by the polymerized complex method are smaller than those for the samples prepared by the solid-state reaction method, which indicates that the doping of the carrier into the material can be more easily realized in the samples prepared by the polymerized complex method. The thermal conductivity decreases with increasing x, but the further suppression of the thermal conductivity was attained by the additional substitution on the Zn site by Mg. The Seebeck coefficient of (Zn1−yMgy)1−xAlxO is almost independent of Mg content y, but the electrical resistivity increases with increasing y. As a result, (Zn0.90Mg0.10)0.9975Al0.0025O shows a maximum dimensionless figure of merit of 0.10 at 1073 K. © 2002 American Institute of Physics.

Electronic Resource

1089-7623

AIP Digital Archive

Physics

Electrical Engineering, Measurement and Control Technology

We have developed a subpicosecond nondegenerate pump-probe spectrometer based on the optical sampling technique using a combination of asynchronous tunable femtosecond lasers. Owing to its simple instrumentation, time evolution of subpicosecond to nanosecond excited molecular processes in solution and of exciton relaxation processes in semiconductor multiple quantum wells has been detected on an oscilloscope by an effective time base magnification of 760 000 times. Fast acquisition of the temporal profiles and ease of wavelength scanning allowed compilation of time-resolved absorption and excitation spectra. A possible extention of the spectrometer with one of the light sources being a synchrotron radiation instead of the femtosecond laser is discussed. © 1999 American Institute of Physics.

Electronic Resource

1365-2133

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Linear IgA bullous dermatosis (LABD) is characterized by IgA autoantibodies against components of the basement membrane zone (BMZ). A 97-kDa protein is one of the major autoantigens associated with this disease. We report a 68-year-old man who developed LABD after a 3-year history of psoriasis and in the context of active hepatitis C virus infection. He had been treated with cyclosporin for psoriasis for about 9 months. Histologically, there was a subepidermal blister containing neutrophils and eosinophils with lymphocytes infiltrating predominantly in the dermis. Direct immunofluorescent staining showed linear IgA deposition at the BMZ. The patient’s IgA autoantibodies bound exclusively to the epidermal side of 1 mol/L salt-split normal human skin. Immunoblot analysis identified a 97-kDa autoantigen in epidermal extracts. This appears to be the first case of LABD with IgA autoantibodies against a 97-kDa autoantigen, associated with psoriasis and hepatitis C virus infection.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

We applied multistrained short-period superlattices (SSPSs) and GaP buffer layers to the InP-on-Si heteroepitaxy, in order to suppress the generation of threading dislocations. As a result, it was found that the density of threading dislocations in an InP/SSPSs/GaAs/SSPSs/GaP/Si structure including (InAs)m(GaAs)n SSPSs and (GaAs)i(GaP)j SSPSs was remarkably reduced, compared with that in the InP/GaP/Si structure. Misfit dislocations lying along the 〈011〉 directions were observed at heterointerfaces in the InP/SSPSs/GaAs/SSPSs/GaP/Si structure. Therefore, the lattice mismatch strain was stepwise accommodated by the generation of misfit dislocations at the heterointerfaces. From these results, it was clarified that multi-SSPSs are effective for reducing the density of threading dislocations in heteroepitaxy with a large lattice mismatch. © 1996 American Institute of Physics.

Electronic Resource

1439-0523

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Sixty soybean cultivars from Japan and the USA formed five maturity groups (IIb-Vc) based on number of days from sowing to flowering and number of days from flowering to maturity. Highly significant intervarietal differences in fatty acid composition were found in all the maturity groups, especially in IIc. Stearic and oleic acids showed a larger variation than palmitic, linoleic and linolenic acids. Principal component analysis suggested that the total variation of fatty acid composition depended mainly on the desaturation levels from oleic to linoleic acid. Three cultivars exhibiting unique fatty acid composition, together with a standard cultivar, were examined for the contents of the five fatty acids, as well as crude oil at eight seed-filling stages. For all four cultivars, it was found that crude oil content increased sigmoidally with advancing filling stage, and that the accumulation patterns of palmitic, linoleic and linolenic acids were similar to that of crude oil. However, the accumulation pattern of stearic acid was different from that of crude oil and divided the cultivars into two distinct groups. For oleic acid, only the cultivar ‘Aburamame’ showed a rapid increase in proportion with advancing filling stage, although not differing markedly in accumulated content from the other cultivars. These results indicate that analysing the accumulation patterns of fatty acids could explain the latent genetic variation in fatty acid composition of soybean seeds.

Electronic Resource

1365-2109

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Vertebral deformities were investigated in cultured red sea bream, Pagrus major. In the field, deformities in seedlings were categorized and their incidence was calculated. In the laboratory, the symptoms of major vertebral deformities were examined morphologically using radiographs and by making transparent skeletal specimens. The internal structure of deformed vertebrae was examined histologically. The shortened body condition had the highest incidence (0.9–8.3%) of all deformities in the seedlings. In individuals with the shortened body condition, the ratio of trunk and caudal part length to body height was smaller. These fish had skeletal anomalies in the vertebrae, mainly centrum defects (64.3%) or undersized centrums (25.2%). The specimens with centrum defects had a characteristic anomaly in the vertebrae, with plural pairs of neural and haemal spines on a single centrum. This anomaly was frequently observed in the posterior abdominal vertebrae. The internal skeletal structure of such abnormal centrums was basically the same as that of normal centrums. In all the specimens with undersized centrums, both the centrum length and diameter were shorter than normal except for the first and second centrum, and urostyle.

Electronic Resource

1365-2222

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Background It has been reported that the toxins that Staphylococcus aureus produces are associated with the exacerbation of atopic dermatitis (AD). It has been shown in many studies that staphylococcal enterotoxin (SE) A and SEB contribute to AD by humoral immunity through IgE production as a superantigen. On the other hand, little attention has been paid to the relationship between AD and exfoliative toxin x (ETx).Objective We investigated the toxins that are frequently detected from the skin of patients and how these toxins affect AD.Methods S. aureus, isolated from the skin of 100 patients with mild to severe AD, were examined for the producibility of toxins by polymerase chain reaction. Serum samples were obtained from 21 patients with mild and moderate AD. The levels of SEB, ETA, total IgE, specific IgE, and specific IgG in sera were measured by ELISA.Results SEB was most frequently detected from S. aureus on the skin of these patients as previously reported. And ETx, to which little attention has been paid so far, was frequently detected next to SEB. Furthermore, ETA was detected from the sera of almost all the AD patients. SEB was not detected at all. Although the level of ETA in the AD group was significantly higher than that of controls, ETA-specific IgE was not detected from their sera. High levels of ETA tended to be detected from infantile patients. Although there were no significant differences in the levels of ETA-IgG between AD and the controls, its prevalence was more than twice as high as the controls in AD.Conclusion These results suggest that many AD patients were exposed to ETx. We conclude that ETx may contribute to exacerbation of AD, particularly in infants, by a mechanism that is not through specific IgE production, unlike SEB.

Electronic Resource

1600-0595

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract Immature permanent teeth often develop complications after luxation injuries. The pathology and the degenerative processes of the complications, however, are not well understood. In this study, we histologically examined the long–term reactions of immature rat teeth after standardized luxation injuries delivered in our previously developed experimental model. The upper first molars of 3-week-old male Wistar rats weighing 50-60 g were pushed horizontally toward the palate to cause a constant amount of dislocation. The follow-up changes of the luxated molars were studied up to 56 days after the trauma. Soon after the trauma, the periodontium had irregular fibers, degenerated cells, hemorrhage and edema in various places. After 12 h, a cell free area appeared in the palatal periodontium. In the alveolar bone and on the surface of the root, resorption occurred one day and 5-7 days after injury respectively. Although the damage in the periodontium and alveolar bone was gradually repaired, the root resorption, especially in the palatal cervical region, continued throughout the experimental period. In the most cases, the dental pulp did not seem to be damaged immediately after the trauma. By the 3rd day after injury, however, displacement of odontoblasts into the dentinal tubules was observed in many teeth. Moreover, formation of irregular dentin was observed in some teeth after 7 days. These changes of the pulp tended to disappear in the later stages of the experiment.

Electronic Resource

1095-8649

Blackwell Publishing Journal Backfiles 1879-2005

Biology

The morphology of scale osteoclasts in rainbow trout Oncorhynchus mykiss was characterized by light and scanning electron microscopy, and the effects of oestradiol-17β-treatment and sexual maturation on scale osteoclast morphology were investigated. The cells associated with resorption cavities could be distinguished morphologically as two types: symmetrical, compact cells lacking or having only a few cell processes, termed type 1 cells, and asymmetrical cells covered with folds and having several cell processes, termed type 2 cells. In adult sexually maturing fish, where scale resorption was high, type 1 cells were predominant. In juveniles and spawned adults where scale resorption was assumed to be relatively low, mostly type 2 cells were present. Oestradiol 17-β-treatment of juvenile rainbow trout increased the osteoclast activity, but did not affect the osteoclast morphology. Using light microscopy, the majority of the cells observed in, and closely associated with, the resorption cavities were mononucleated in both maturing and spawned fish. Occasionally, bi- and multinucleated osteoclasts were observed in the maturing, but not in the spawned fish. Light microscopic enzyme-histochemistry showed that the majority of the mononucleated cells, as well as the bi- and multinucleated ones, were tartrate resistant acid phosphatase positive in both groups of fish, thus implying that both type 1 and type 2 cells were osteoclasts. It is thus apparent that scale resorption in rainbow trout is carried out by two morphologically distinct osteoclast populations, representing different stages of osteoclast activity and/or different stages of osteoclast differentiation.

Electronic Resource

1600-0714

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract Deciduous teeth affected by dentinogenesis imperfecta were obtained from two patients with osteogenesis imperfecta. Electronmicroscopy of the dentin revealed some structural alterations. The striation of the collagen fibrils was not clear, and the crystals were less dense than in normal dentin. The amino acid analysis of dentin collagen was slightly different from normal dentin. There were slight increases in the amounts of hydroxylysine. serine and acidic acids. On the other hand, lysine and arginine were reduced. The elevation of hexosyllysine content and an increase of carbohydrate were also observed.These results indicate that either dentin collagen is altered, or noncollagenous matrices associated with collagen are increased in dentinogenesis imperfecta dentin.

Electronic Resource

1600-0714

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Deciduous teeth from 7 patients with dentinogenesis imperfecta (DI) Type I and Type II were examined by conventional microscopy. A defective layer was found which runs parallel with the dentinal surface in the outer portion of dentin in teeth of both types. Dentinal tubules were interrupted in the vicinity of this layer. When the ground sections were examined after being stained by the phosphophoryn staining method, the DI Type I dentin was found to contain phosphophoryn at the same low level as the DI Type II dentin, suggesting similar deficiency in phosphophoryn concentration. These results suggest that both types of DI have a common primary disturbance in the early stage of odontoblast differentiation.

Electronic Resource

1600-0714

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Dentin phosphophoryn is a highly phosphorylated protein which has a hydrophilic character but is not soluble in dilute acetic acid. A histochemical method was developed for staining this protein with Stains-all® in situ utilizing those chemical properties. We have succeeded in detecting the presence of this protein in circumpulpal orthodentin of human permanent and deciduous teeth, but not in mantle dentin, secondary dentin and reparative dentin. Phosphophoryn staining was also absent in the dentin of dentinogenesis imperfecta (DI) Type II, a genetic disorder of dentin formation. From these results, it is suggested that phosphophoryn is synthesized and secreted only by physiologically-differentiated odontoblasts and that the mineralization processes of mantle, secondary, reparative and DI dentins may be different from that of circumpulpal orthodentin.

Electronic Resource

0167-4781

(C. carpio) ; Cloning ; Nucleotide sequence ; α-Globin mRNA

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

1398-9995

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource