Search Results - (Author, Cooperation:Y. Ogura)
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1Latest Papers from Table of Contents or Articles in PressKimura, M., Araoka, H., Yamamoto, H., Nakamura, S., Nagi, M., Yamagoe, S., Miyazaki, Y., Ogura, S., Mitsuki, T., Yuasa, M., Kaji, D., Kageyama, K., Nishida, A., Taya, Y., Shimazu, H., Ishiwata, K., Takagi, S., Yamamoto, G., Asano-Mori, Y., Uchida, N., Wake, A., Taniguchi, S., Yoneyama, A.
The American Society for Microbiology (ASM)
Published 2018Staff ViewPublication Date: 2018-04-27Publisher: The American Society for Microbiology (ASM)Print ISSN: 0066-4804Electronic ISSN: 1098-6596Topics: BiologyMedicinePublished by: -
2Latest Papers from Table of Contents or Articles in PressTanaka, Y., Sabharwal, L., Ota, M., Nakagawa, I., Jiang, J.-J., Arima, Y., Ogura, H., Okochi, M., Ishii, M., Kamimura, D., Murakami, M.
The American Association of Immunologists (AAI)
Published 2018Staff ViewPublication Date: 2018-10-10Publisher: The American Association of Immunologists (AAI)Print ISSN: 0022-1767Electronic ISSN: 1550-6606Topics: MedicinePublished by: -
3Latest Papers from Table of Contents or Articles in PressY. Saitoh ; H. Suzuki ; K. Tani ; K. Nishikawa ; K. Irie ; Y. Ogura ; A. Tamura ; S. Tsukita ; Y. Fujiyoshi
American Association for the Advancement of Science (AAAS)
Published 2015Staff ViewPublication Date: 2015-02-14Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Claudins/*chemistry ; Enterotoxins/*chemistry ; Hydrophobic and Hydrophilic Interactions ; Mice ; Protein Structure, Secondary ; Tight Junctions/chemistry/*ultrastructurePublished by: -
4Latest Papers from Table of Contents or Articles in PressT. Horie ; R. Shinki ; Y. Ogura ; T. G. Kusakabe ; N. Satoh ; Y. Sasakura
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-01-05Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; *Cell Differentiation ; Central Nervous System/cytology/growth & development ; Larva ; Metamorphosis, Biological ; Neural Stem Cells/cytology ; Urochordata/*growth & developmentPublished by: -
5Latest Papers from Table of Contents or Articles in PressStaff View
Publication Date: 2018-03-06Publisher: Royal SocietyElectronic ISSN: 2054-5703Topics: Natural Sciences in GeneralKeywords: optics, biomedical engineeringPublished by: -
6Articles: DFG German National LicensesKimura, M. ; Takahashi, T. ; Iwata, A. ; Matsumoto, N. ; Ogura, Y. ; Akagi, T. ; Akima, S. ; Kobayashi, K. ; Moro, I.
Oxford, UK : Blackwell Science Ltd
Published 2001Staff ViewISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: We used Northern blot analysis in order to investigate the ontogeny of the murine joining (J)-chain gene. No J-chain expression was detected in embryonic tissues, including liver, spleen and intestine, but an expression of µ heavy chain was detected in foetal liver at day 17. J-chain expression was detected in the spleen at day 9 and in the intestine at day 15 after birth. Western blot analysis was carried out in order to compare the protein levels of J and µ heavy chains in serum from day 8 to day 24 after birth, using antihuman J chain and antimouse µ chain antibodies. Although µ chain protein could be detected in serum from day 8, J-chain protein was detectable only at day 24. These results suggest that the expression of J chain is a later event than the µ chain in the mouse, which thus differs in embryogenesis from humans.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesMatsumoto, N. ; Asano, M. ; Ogura, Y. ; Takenouchi-Ohkubo, N. ; Chihaya, H. ; Chung-Hsing, W. ; Ishikawa, K. ; Zhu, L. ; Moro, I.
Oxford, UK : Blackwell Science Ltd
Published 2003Staff ViewISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Using a recombinant vaccinia virus containing the T7 RNA polymerase, we have established a system for the transient expression of human polymeric immunoglobulin receptor (pIgR) in baby hamster kidney cells, a baby hamster-derived fibroblastic cell line. This transfection system resulted in the successful expression of pIgR in these cells, and Western blot analysis showed that human pIgR was expressed as two different molecular weight forms of 92 and 107 kDa. Treatment with endoglycosidase H showed that the difference between these two forms was due to the glycosylation status of the protein. In order to examine the functional role of glycosylation, we treated the transfected cells with tunicamycin, which prevents a core glycosylation step in the endoplasmic reticulum. Non-glycosylated pIgR was released into the culture medium of the transfected cells, albeit with extremely low efficiency. Taking these results together, we conclude that the glycosylation of pIgR may play a positive role in the efficient transport or release of free pIgR.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesYONEDA, K. ; OKAMOTO, H. ; WADA, Y. ; MORITA, K. ; TAKAHASHI, M. ; OGURA, Y. ; IMAMURA, S.
Oxford, UK : Blackwell Publishing Ltd
Published 1995Staff ViewISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Summary Ocular complications of atopic dermatitis include cataract, blepharitis, keratoconjunctivitis, keratoconus, iritis and retinal detachment. The aim of this study was to evaluate the characteristics of retinal detachment in atopic dermatitis patients. We examined four patients with atopic dermatitis and retinal detachment, and performed an extensive review of the literature. There have been about 130 reported cases of retinal detachment in patients with atopic dermatitis from Japan, in comparison with only a few reports from Europe and the U.S.A. An extensive review of the literature revealed that retinal detachment occurs at a young age in atopic dermatitis patients, and that often both eyes are involved. As retinal detachment is not a rare complication of atopic dermatitis, we propose that this type of retinal detachment is designated ‘atopic retinal detachment’. Dermatologists should be aware of this potential complication of atopic dermatitis.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesAmano, S. ; Akutsu, N. ; Ogura, Y. ; Nishiyama, T.
Oxford, UK : Blackwell Science Ltd
Published 2004Staff ViewISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background Laminin 5 is known to induce the adhesion, spreading and migration of human keratinocytes. In skin wound healing, laminin 5 deposition beneath migrating keratinocytes occurs early and is followed by the formation of hemidesmosomes and then basement membrane.Objectives To identify factors that regulate the synthesis and secretion of laminin 5 by human keratinocytes during acute wound healing.Methods Laminin 5 synthesis by human keratinocytes was determined by a specific sandwich enzyme-linked immunosorbent assay. To determine the total amount of laminin 5 synthesized, laminin 5 deposited on culture dishes and inside cells was solubilized by detergent solution and determined separately from conditioned medium, and the total laminin 5 synthesis was calculated. A quantitative polymerase chain reaction method was used to measure the expression levels of laminin 5 genes, LAMA3, LAMB3 and LAMC2, which correspond to the α3, β3 and γ2 chains of laminin 5. We also examined the effects of lysophospholipids, proinflammatory cytokines and growth factors, which are components in acute wound fluids, on laminin 5 synthesis in keratinocytes.Results Human acute wound fluid at days 1, 2 and 3 stimulated laminin 5 synthesis in cultured human keratinocytes in a concentration-dependent manner, although findings are restricted to one case. Human serum also increased laminin 5 production by human keratinocytes as strongly as the wound fluid did, suggesting that the major active components in acute wound fluid may be derived from those in human serum. Lysophospholipids such as lysophosphatidic acid (LPA), lysophosphatidylcholines (LPCs) and sphingosine-1-phosphate (S1P) increased laminin 5 synthesis in a concentration-dependent manner. Among growth factors, epidermal growth factor, insulin-like growth factor-1, interferon-γ and keratinocyte growth factor increased laminin 5 production in keratinocytes, while platelet-derived growth factor, hepatocyte growth factor and basic fibroblast growth factor were ineffective. Although interleukin-1α had no effect, transforming growth factor (TGF)-α, tumour necrosis factor (TNF)-α and TGF-β1 also stimulated laminin 5 synthesis, and TGF-α and TGF-β1 showed a synergistic effect. Neutralizing antibodies to TGF-α and TGF-β1 markedly inhibited the enhanced laminin 5 synthesis by human serum, suggesting that TGF-α and TGF-β1 are important components to increase laminin 5 in human serum. In line with the increase of laminin 5 synthesis, the expression levels of all three laminin 5 genes were also augmented by TGF-α and TGF-β1.Conclusions Laminin 5 synthesis in human keratinocytes was augmented by inflammatory cytokines and growth factors such as TGF-α, TGF-β1 and TNF-α, and lysophospholipids such as S1P, LPA and LPCs, which are supposed to be present in acute wound fluid. The increased laminin 5 protein in the wound area presumably enhances wound repair by stimulating adhesion and migration of keratinocytes on the wound bed and by facilitating basement membrane formation at the dermal–epidermal junction.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesAmano, S. ; Ogura, Y. ; Akutsu, N. ; Matsunaga, Y. ; Kadoya, K. ; Adachi, E. ; Nishiyama, T.
Oxford, UK : Blackwell Publishing Ltd
Published 2005Staff ViewISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background The epidermal basement membrane (BM) plays important roles in adhesion between epidermis and dermis, and in controlling epidermal differentiation. The BM has been reported to be damaged in sun-exposed skin. Although matrix metalloproteinases (MMPs) are believed to be involved in the BM damage, there is no good in vitro model for examining BM damage by MMPs or for exploring methods to protect the BM.Objectives To examine the involvement of MMPs in BM damage and approaches to protect the BM from such damage by using an in vitro skin-equivalent (SE) model.Method SE was prepared by culturing human keratinocytes on contracted collagen gel including human fibroblasts. MMP-1, -2, -3 and -9, laminin 5 and type IV and VII collagens were determined by specific sandwich ELISAs, and MMP-2 and MMP-9 were analysed by gelatin zymography. Histological examination of SE was also carried out.Results Despite production of BM components such as laminin 5 and type IV and VII collagens in SEs, BM was rarely observed at the dermal–epidermal junction. Several MMPs, such as MMP-1, -2, -3 and -9, were observed to be present in conditioned media and some of them were in active forms. Tissue inhibitor of metalloproteinase (TIMP)-2 was not detected, although TIMP-1 was present. Synthetic MMP inhibitors, CGS27023A and MMP-inhibitor I, which inhibit MMP-1, -2, -3 and -9, markedly augmented deposition of laminin 5 and type IV and VII collagens at the dermal–epidermal junction, resulting in the formation of continuous epidermal BM.Conclusions Our results indicate that MMPs are involved in the degradation of BM in SEs, and that MMP inhibitors exert a protective effect against BM damage.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 0005-2760Keywords: (Rat) ; 3α,7α-Dihydroxy-5β-cholestane ; Bile acid metabolism ; Cholic acid ; Diabetes ; VanadateSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 0005-2760Keywords: (Rat) ; 3α,7α-Dihydroxy-5β-cholestane ; Bile acid metabolism ; Perfused liverSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 0005-2744Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 0009-8981Keywords: Colorimetric method ; Methylene blue ; Peroxidase-coupled reaction ; Serum diamine oxidaseSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: MedicineType of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesMaeda, Y. ; Trautwein, A. ; Gonser, U. ; Yoshida, K. ; Kikuchi-Torii, K. ; Homma, T. ; Ogura, Y.
Amsterdam : ElsevierStaff ViewISSN: 0005-2795Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 0003-2697Keywords: 16α-hydroxylase ; 2-hydroxylase ; HPLC ; QAE-Sephadex A-25, borate form ; estradiol ; voltametric detectionSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 0003-2697Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 0022-2364Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesStaff View
ISSN: 0022-2364Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesStaff View
ISSN: 0022-2364Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: