Search Results - (Author, Cooperation:Y. Nam)

2018-11-14

The American Society for Pharmacology and Experimental Therapeutics (ASPET)

0026-895X

1521-0111

Chemistry and Pharmacology

Medicine

2011-01-18

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Catalytic Domain ; Humans ; *Models, Molecular ; N-Acetylglucosaminyltransferases/*chemistry/metabolism ; Peptides/chemistry/metabolism ; Protein Structure, Tertiary ; Uridine Diphosphate/metabolism

2018-06-02

The American Association for Cancer Research (AACR)

0008-5472

1538-7445

Medicine

2018-10-19

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Geosciences

Computer Science

Medicine

Natural Sciences in General

Physics

Physics

1439-0264

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

The expression of calbindin D-28k (CB), calretinin (CR), substance P (SP) and calcitonin gene-related peptide (CGRP) in the stomach myenteric plexus of the Korean native goat stomach was investigated by immunohistochemistry. The results demonstrated the presence of nerve fibers and cell bodies immunoreactive (IR) to CB, CR, SP and CGRP. In tissues of rumen, reticulum, omasum and abomasum, some distinct neuronal populations could be distinguished according to their morphologic and neuronal chemical properties: Dogiel type I cells which have irregular lamellar dendrites and a single axon, Dogiel type II cells which have large ovoid cell bodies and several long axon-like processes, and small filamentous interneurons. CB-, CR-, SP- and CGRP-IR neurons and fibers were observed in the myenteric plexus of stomach, and varicose nerve fiber immunostained to SP and CGRP also were found in the muscle layer. In myenteric plexus of the stomach, CB- and SP-positive neurons were characterized by Dogiel type II and CR-IR neurons were classified Dogiel type I with lamellar dendrites, and immunoreactivity of CGRP was very weak in the somata. SP- and CGRP-IR nerve fibers formed dense networks within the myenteric ganglia. SP-IR cell bodies and their fibers were found in the myenteric plexus, and the immunoreactivity and number of cell bodies were more than CB-, CR-, and CGRP-IR neurons. These results suggest that SP, CGRP, CB and CR in the myenteric neurons of Korean native goat stomach may have play an important role in the dynamic movement.(Support contributed by: Korean Research Foundation 2003-015-E00195).

Electronic Resource

1365-2109

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Viable interspecific hybrids between yellowtail flounder (Pleuronectes ferrugineus, Storer) and winter flounder (Pleuronectes americanus, Walbaum) were produced by artificial insemination of yellowtail flounder eggs with winter flounder sperm. However, mean fertilization rate, hatching success and early survival up to 3 weeks post hatch were significantly lower than those of parental pure cross controls (P 〈 0.01). Overall, cytogenetic traits (karyological analysis and estimation of cellular DNA contents using flow cytometry) of hybrid flounder were intermediate between the two parental species. Microsatellite assay was used to distinguish the parental genomes in the hybrids; in most cases, one allele was specific to each of the parents. Morphometrics assessed by body proportions indicated that hybrids generally displayed a morphology intermediate between the maternal and paternal species. Interspecific hybrids exhibited abnormal and retarded gonad development in both sexes based on histological analysis of gonads from adult fish. The sterility of the hybrids presents a significant advantage for their use in aquaculture, as potential escapees would not be capable of reproducing in the wild and contaminating natural stocks.

Electronic Resource

1365-2109

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Mud loach, Misgurnus mizolepis (Günther), were sex reversed using the immersion technique. Fry were raised in water containing 50, 100 or 200 μg oestradiol-17β I−1 for 1, 2 or 3 weeks. Survival rates decreased as dose and duration of treatment increased: in most instances they were significantly (P 〈 0.05) lower than those of the controls. Growth rates were unaffected except for fish in the 200 μg I−1 treatments. The 200 μg T−1 treatments for 2 and 3 weeks produced monosex female populations. The 100 μg I−1 treatment for 3 weeks produced 99% females and 1% intersex individuals. Histologically, ovaries of sex-reversed fish were similar to those of normal females. Morphologically, however, they were different in that the ovaries of sex-reversed fish were not connected to the genital pore. The hormone treatments affected the size and morphology of the pectoral fins, which exhibit sexual dimorphism in mud loach.

Electronic Resource

1365-2109

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Immersion of 1-day-old Silurus asotus (Linnaeus) larvae in 50, 100 or 200 μg L−1 oestradiol-17β for 15 days altered gonad differentiation and gonadal morphology. The survival of the group immersed at the highest dose (200 μg L−1) was reduced significantly (86%), whereas survival of the groups immersed at the lower doses was as high as that of untreated controls (94%). The sex ratio of the untreated group was close to 1:1 (female–male); however, the incidence of females was significantly higher in groups immersed in doses of 50, 100 and 200 μg L−1, i.e. 86%, 92% and 99.4%, respectively. Ovarian morphology of feminized individuals was different to that of the controls; the ovary was morphologically rather similar to testis although it contained fully matured oocytes. Morphological changes in genital organs were also noted in fish exposed to oestradiol-17β the shape and size of genital papilla were intermediate between those of normal male and female. During a 4-month growth trial after the exposure, the feminized fish showed significantly improved growth performance over normal genetic males (P 〈 0.05), although the growth was not equal to that of normal genetic females (P 〈 0.05).

Electronic Resource

1749-7345

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

For the evaluation of hormonal control of spermiation in fish, a method to quanify the spermiation response of mature Rhynchocypris oxycephalus (Sauvage and Dabry) to hormonal therapy is described. Spermatocrit was determined after 7 min centrifugation at 18,000 ±g and sperm density was estimated by a standard hemocytomer method. Sperm density can be predicted from spermatocrit since their relationship is linear as described by the regression equation, Y = 3.68X - 27.18 (R2= 0.82, N= 50). where Y is spermatocrit and × is sperm density. Milt production by mature R. oxycephalus was highest at 24 h after injection of 1,000 IU human chorionic gonadotropin (HCG) and 50 μg luteinizing hormone-releasing hormone analogue (LHRHa) per kg body weight. Increased milt production coincided with low spermatocrit and sperm density levels. These results demonstrate that spermiation in mature R. oxycephalus can be reliably evaluated by a spermatocrit method and that HCG and LHRHa are effective in stimulating of spermiation in this species.

Electronic Resource

1439-0264

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Recent in vitro and in vivo studies have shown that glucocorticoids have a profound influence on the survival of hippocampal neurones, and that the depletion of glucocorticoids as a result of adrenalectomy (ADX) reduces nerve growth factor levels in the hippocampus. It is also believed that ADX is associated with the seizure susceptibility of the Mongolian gerbil. In the present study, the choronological changes of c-jun immunoreactivity were investigated after ADX in the hippocampal formations in the seizure-prone gerbil model. In the sham hippocampus, c-jun immunoreactivity was not observed in the neurones of the hippocampus proper and dentate gyrus. C-jun immunoreactive neurones appeared 3 h after ADX in the neurones of the CA1 area and dentate gyrus, and these immunoreactivities peaked 24 h after ADX and then gradually decreased. These results suggest that, in the adrenalectomized gerbil, c-jun may be expressed in the neurones of the hippocampus in compensation for glucocorticoid deficit. The result of enhanced c-jun expression of the hippocampal formation provides anatomical support for the hypothesis that c-jun may play a role in the reduction of seizure activity.

Electronic Resource

0272-7757

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Education

Economics

Electronic Resource

0308-8146

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Process Engineering, Biotechnology, Nutrition Technology

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary.  The contributions of the near N-terminal residues of Rev protein of HIV were investigated by analyzing N-terminal deletions of Rev in the context of a Rev/MS-C fusion protein that can bind and activate both the Rev responsive element (RRE) and the MS2 phage translational operator RNAs. Rev/MS-C fusion proteins deleted for residues 3–19 of Rev retained trans-activation potential for both RRE and MS2 targets. Coincidentally, peptides spanning residues 17–87 or 22–85 were functionally competent for trans-activation of RRE containing HIV-1 gag mRNA. Deletion of residues 18–24 of Rev in the Rev/MS-C fusion protein abolished the activation potential for both RRE and MS2 targets, although this mutant was competent for specific RNA binding, protein multimerization, and nuclear and nucleolar localization. Four mutants dominantly interfering with Rev activation of RRE were mapped near the N-terminus of Rev; (i) between residues 18 and 24, (ii) 25–34, (iii) 43–50, and (iv) 51–60. Of these, the mutant lacking residues 18–24 was a novel trans-dominant inhibitor of Rev and Rev/MS-C for activation of RRE or MS2 RNA, while the oligomerization domain mutants mapping between residues 25–34 or 51–60 inhibited the activation of RRE rather than MS2 RNA.

Electronic Resource

1432-2242

Key words Medicago truncatula ; BAC library ; PCR-amplification screening ; Nodulation ; Ethylene-response genes

Springer Online Journal Archives 1860-2000

Biology

Abstract  To facilitate genome analysis and map-based cloning of symbiotic genes in the model legume Medicago truncatula, a bacterial artificial chromosome (BAC) library was constructed. The library consists of 30 720 clones with an average insert size of approximately 100 kb, representing approximately five haploid-genome equivalents. The frequency of BAC clones carrying inserts of chloroplast DNA was estimated to be 1.4%. Screening of the library with single- or low-copy genes as hybridization probes resulted in the detection of 1–12 clones per gene. Hybridization of the library with repeated sequences such as rDNA genes and transposon-like elements of M. truncatula revealed the presence of 60 and 374 BAC clones containing the two sequences, respectively. The BAC library was pooled for screening by polymerase chain reaction (PCR)-amplification. To demonstrate the utility of this system, we used primers designed from a conserved region of the ein3-like loci of Arabidopsis thaliana and isolated six unique BAC clones from the library. DNA gel-blot and sequence analyses showed that these ein3-like clones could be grouped into three classes, an observation consistent with the presence of multiple ein3-like loci in M. truncatula. These results indicate that the BAC library represents a central resource for the map-based cloning and physical mapping in M. truncatula and other legumes.

Electronic Resource