Search Results - (Author, Cooperation:X. D. Fu)
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1Latest Papers from Table of Contents or Articles in PressD. Wang ; I. Garcia-Bassets ; C. Benner ; W. Li ; X. Su ; Y. Zhou ; J. Qiu ; W. Liu ; M. U. Kaikkonen ; K. A. Ohgi ; C. K. Glass ; M. G. Rosenfeld ; X. D. Fu
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-05-17Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Base Sequence ; Cell Line, Tumor ; Cell Lineage ; Dihydrotestosterone/pharmacology ; Down-Regulation ; Enhancer Elements, Genetic/*genetics ; Gene Expression Regulation, Neoplastic ; Gene Knockdown Techniques ; Genome, Human/genetics ; HEK293 Cells ; Hepatocyte Nuclear Factor 3-alpha/deficiency/genetics/*metabolism ; Histones/metabolism ; Humans ; Kallikreins ; Male ; Prostate-Specific Antigen ; Prostatic Neoplasms/metabolism/pathology ; RNA, Small Interfering/genetics/metabolism ; RNA, Untranslated/*genetics ; Receptors, Androgen/*metabolism ; Transcription, Genetic/*geneticsPublished by: -
2Latest Papers from Table of Contents or Articles in PressW. Wang ; J. Y. Barnaby ; Y. Tada ; H. Li ; M. Tor ; D. Caldelari ; D. U. Lee ; X. D. Fu ; X. Dong
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-02-05Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Apoptosis ; Arabidopsis/*immunology/*microbiology/physiology ; Arabidopsis Proteins/*metabolism ; Circadian Clocks/*immunology ; Circadian Rhythm/genetics/immunology ; Gene Expression Regulation, Plant/genetics/immunology ; Genes, Plant/genetics/immunology ; Immunity, Innate/immunology ; Light ; Mutation ; Oomycetes/*immunology/pathogenicity ; Phenotype ; Plant Diseases/genetics/*immunology/microbiology ; Plant Immunity/*immunology ; Spores, Fungal/immunology/physiology ; Time Factors ; Transcription Factors/*metabolismPublished by: -
3Latest Papers from Table of Contents or Articles in PressL. Wang ; Y. Zhou ; L. Xu ; R. Xiao ; X. Lu ; L. Chen ; J. Chong ; H. Li ; C. He ; X. D. Fu ; D. Wang
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-07-01Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Crystallography, X-Ray ; Cytosine/*analogs & derivatives/chemistry/metabolism ; DNA Methylation ; DNA Repair ; Epigenesis, Genetic ; Hydrogen Bonding ; Kinetics ; RNA Polymerase II/*chemistry/*metabolism ; Saccharomyces cerevisiae/*enzymology/genetics/metabolism ; Substrate Specificity ; Templates, Genetic ; Thymine DNA Glycosylase/metabolism ; *Transcription Elongation, GeneticPublished by: -
4Latest Papers from Table of Contents or Articles in PressH. Ouyang ; Y. Xue ; Y. Lin ; X. Zhang ; L. Xi ; S. Patel ; H. Cai ; J. Luo ; M. Zhang ; Y. Yang ; G. Li ; H. Li ; W. Jiang ; E. Yeh ; J. Lin ; M. Pei ; J. Zhu ; G. Cao ; L. Zhang ; B. Yu ; S. Chen ; X. D. Fu ; Y. Liu ; K. Zhang
Nature Publishing Group (NPG)
Published 2014Staff ViewPublication Date: 2014-07-18Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Cell Differentiation ; Cell Lineage ; Corneal Diseases/*metabolism/*pathology ; Disease Models, Animal ; Epithelium, Corneal/*cytology/*metabolism/pathology ; Eye Proteins/genetics/*metabolism ; Homeodomain Proteins/genetics/*metabolism ; *Homeostasis ; Humans ; Limbus Corneae/cytology/metabolism ; Male ; Paired Box Transcription Factors/genetics/*metabolism ; Rabbits ; Repressor Proteins/genetics/*metabolism ; Signal Transduction ; Skin/cytology/metabolism/pathology ; Stem Cell Transplantation ; Stem Cells/cytology/metabolism ; Transcription Factors/metabolism ; Tumor Suppressor Proteins/metabolism ; Wnt Proteins/genetics/*metabolismPublished by: -
5Latest Papers from Table of Contents or Articles in PressO. Harismendy ; D. Notani ; X. Song ; N. G. Rahim ; B. Tanasa ; N. Heintzman ; B. Ren ; X. D. Fu ; E. J. Topol ; M. G. Rosenfeld ; K. A. Frazer
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-02-11Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Alleles ; Cell Line ; Chromatin/drug effects/genetics/metabolism ; Chromosomes, Human, Pair 9/*genetics ; Conserved Sequence/genetics ; Coronary Artery Disease/*genetics ; Cyclin-Dependent Kinase Inhibitor p15/genetics ; Diabetes Mellitus, Type 2/genetics ; Endothelial Cells/drug effects/metabolism ; Enhancer Elements, Genetic/*genetics ; European Continental Ancestry Group/genetics ; Gene Expression Regulation/drug effects/genetics ; Gene Knockdown Techniques ; Genetic Predisposition to Disease/*genetics ; *Genetic Variation ; Genome-Wide Association Study ; Haplotypes/genetics ; HeLa Cells ; Humans ; Interferon-alpha/genetics ; Interferon-gamma/*pharmacology ; Linkage Disequilibrium ; Male ; Polymorphism, Single Nucleotide/genetics ; Protein Binding/drug effects ; Purine-Nucleoside Phosphorylase/genetics ; STAT1 Transcription Factor/biosynthesis/deficiency/genetics/metabolism ; Signal Transduction/*drug effectsPublished by: -
6Articles: DFG German National LicensesStaff View
ISSN: 0378-1119Keywords: Oligodeoxynucleotide-directed deletion mutagenesis ; phage λ p"l promoter ; phosphorylation ; recombinant DNASource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyType of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesKopecny, V. ; Biggiogera, M. ; Laurincik, J. ; Pivko, J. ; Grafenau, P. ; Martin, T. E. ; Fu, X. D. ; Fakan, S.
Springer
Published 1996Staff ViewISSN: 1432-0886Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract The fine structure of pig oocytes at the germinal vesicle (GV) stage and early preimplantation embryos (one to four blastomeres) isolated at slaughter was investigated by cytochemical and immunocytochemical methods. The distribution of nucleic acids and ribonucleoproteins (RNPs) in “compact nucleoli” [denominated nucleolus-like bodies (NLB) in oocytes and nucleolus precursor bodies (NPB) in early embryos] and in intranuclear bodies or granules was investigated by staining methods preferential for nuclear RNPs or using the osmium ammine or ethidium bromide-phosphotungstic acid (EB-PTA) reactions for nucleic acids. The distributions of the Sm antigen of nucleoplasmic small nuclear RNPs (snRNPs), the methyl-3 guanosine (m3G) cap of snRNAs and the splicing factor SC-35 were detected by immunoelectron microscopy using specific antibodies. The RNP nature of both NLBs and NPBs, and of nuclear granules in oocytes and embryos, and of fibrillar strands radially projecting from NLBs was revealed. Cytochemical evidence for RNA as a component of NLBs was further provided by EB-PTA staining in combination with the enzymatic removal of RNA, or by osmium-ammine staining without previous acid hydrolysis, while the absence of DNA in NLBs was established by Feulgen-like osmium-ammine staining. In addition, autoradiography demonstrated the absence of [6-3H]thymidine incorporation into NPBs. Other autoradiographic evidence attested the accumulation of RNA in NLBs of oocytes after a 60 min in vitro pulse of [5-3H]uridine. Immunoelectron microscopy using specific antibodies revealed the occurrence of nucleoplasmic snRNPs in both NLBs and NPBs. The presence of snRNA in NLB was confirmed by means of an antibody recognizing the m3G-cap structure. Another spliceosomal component, the protein SC-35 was also detected in NLBs. Among the numerous and variable intranuclear granules occurring mostly in aggregates, the Sm antigen was clearly detected only in the interchromatin granule-type component. Some Sm labeling was occasionally seen in other categories of larger granules. No reaction was detected over any granules when using the anti-m3G-cap antibody. The aggregates consisting of large granules and a finely fibrillar component were intensely immunolabeled by the anti-SC-35 splicing factor probe. Our observations suggest that the compact nucleoli, known to be present before and after fertilization in mammals (NLBs of oocytes and NPBs of early embryos), represent nuclear structural elements containing nonnucleolar, spliceosomal components.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesKopecny, V. ; Biggiogera, M. ; Laurincik, J. ; Pivko, J. ; Grafenau, P. ; Martin, T. E. ; Fu, X. D. ; Fakan, S.
Springer
Published 1996Staff ViewISSN: 1432-0886Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract. The fine structure of pig oocytes at the germinal vesicle (GV) stage and early preimplantation embryos (one to four blastomeres) isolated at slaughter was investigated by cytochemical and immunocytochemical methods. The distribution of nucleic acids and ribonucleoproteins (RNPs) in ”compact nucleoli” [denominated nucleolus-like bodies (NLB) in oocytes and nucleolus precursor bodies (NPB) in early embryos] and in intranuclear bodies or granules was investigated by staining methods preferential for nuclear RNPs or using the osmium ammine or ethidium bromide-phosphotungstic acid (EB-PTA) reactions for nucleic acids. The distributions of the Sm antigen of nucleoplasmic small nuclear RNPs (snRNPs), the methyl-3 guanosine (m3G) cap of snRNAs and the splicing factor SC-35 were detected by immunoelectron microscopy using specific antibodies. The RNP nature of both NLBs and NPBs, and of nuclear granules in oocytes and embryos, and of fibrillar strands radially projecting from NLBs was revealed. Cytochemical evidence for RNA as a component of NLBs was further provided by EB-PTA staining in combination with the enzymatic removal of RNA, or by osmium-ammine staining without previous acid hydrolysis, while the absence of DNA in NLBs was established by Feulgen-like osmium-ammine staining. In addition, autoradiography demonstrated the absence of [6-3H]thymidine incorporation into NPBs. Other autoradiographic evidence attested the accumulation of RNA in NLBs of oocytes after a 60 min in vitro pulse of [5-3H]uridine. Immunoelectron microscopy using specific antibodies revealed the occurrence of nucleoplasmic snRNPs in both NLBs and NPBs. The presence of snRNA in NLB was confirmed by means of an antibody recognizing the m3G-cap structure. Another spliceosomal component, the protein SC-35 was also detected in NLBs. Among the numerous and variable intranuclear granules occurring mostly in aggregates, the Sm antigen was clearly detected only in the interchromatin granule-type component. Some Sm labeling was occasionally seen in other categories of larger granules. No reaction was detected over any granules when using the anti-m3G-cap antibody. The aggregates consisting of large granules and a finely fibrillar component were intensely immunolabeled by the anti-SC-35 splicing factor probe. Our observations suggest that the compact nucleoli, known to be present before and after fertilization in mammals (NLBs of oocytes and NPBs of early embryos), represent nuclear structural elements containing nonnucleolar, spliceosomal components.Type of Medium: Electronic ResourceURL: