Search Results - (Author, Cooperation:W. de Laat)

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  1. 1
    D. Noordermeer ; M. Leleu ; E. Splinter ; J. Rougemont ; W. De Laat ; D. Duboule
    American Association for the Advancement of Science (AAAS)
    Published 2011
    Staff View
    Publication Date:
    2011-10-15
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; Chromatin/metabolism/ultrastructure ; Embryo, Mammalian/cytology/*metabolism ; Gene Expression Regulation, Developmental ; *Genes, Homeobox ; Histones/metabolism ; Mice ; Models, Genetic ; *Multigene Family ; Transcription, Genetic ; *Transcriptional Activation
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    W. de Laat ; D. Duboule
    Nature Publishing Group (NPG)
    Published 2013
    Staff View
    Publication Date:
    2013-10-25
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; DNA/chemistry/genetics/metabolism ; Enhancer Elements, Genetic/*genetics ; Evolution, Molecular ; Gene Expression Regulation, Developmental/*genetics ; Genome/genetics ; Humans ; Mammals/*genetics ; Nucleic Acid Conformation ; Promoter Regions, Genetic/genetics
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Staff View
    Publication Date:
    2013-07-26
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; Binding Sites ; Cell Line ; Chromatin/*chemistry/genetics/*metabolism ; Chromatin Immunoprecipitation ; *Chromosome Positioning ; Embryonic Stem Cells/cytology/metabolism ; Enhancer Elements, Genetic ; Genome/*genetics ; Homeodomain Proteins/metabolism ; *Imaging, Three-Dimensional ; Induced Pluripotent Stem Cells/cytology/metabolism ; Mice ; Molecular Imaging ; Octamer Transcription Factor-3/metabolism ; Organ Specificity ; Pluripotent Stem Cells/*cytology/*metabolism ; Promoter Regions, Genetic ; SOXB1 Transcription Factors/metabolism
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  4. 4
    Bierman, Arjo J. ; Koenderman, Leo ; Tool, Anton J. ; Siegfried, W. De Laat

    New York, NY [u.a.] : Wiley-Blackwell
    Published 1990
    Staff View
    ISSN:
    0021-9541
    Keywords:
    Life and Medical Sciences ; Cell & Developmental Biology
    Source:
    Wiley InterScience Backfile Collection 1832-2000
    Topics:
    Biology
    Medicine
    Notes:
    Swiss 3T3 cells express receptors for both the polypeptide epidermal growth factor (EGF) and the tetradecapeptide bombesin and respond mitogenically to these substances. These cells thus provide a system to analyze potential signal transduction pathways involved in mitogenic stimulation. Here we have determined and compared the early ionic responses elicited by EGF and bombesin and their relation to diacylglycerol (DG) and inositolphosphate (InsPn) production. Whereas EGF fails to cause any significant change in intracellular Ca2+ bombesin effectively induces prompt and transient Ca2+ mobilization from intracellular stores. Further support of the idea that these receptors utilize distinct signalling pathways comes from the measurements of cytoplasmic pH (pHi). As in most target cells, EGF induces a delayed (1 min) but sustained intracellular alkalinization that reaches a new steady state after ∼10 min. Bombesin, in contrast, elicits a biphasic response; within seconds, a rapid but transient rise in pHi is observed, followed by a further slower sustained alkalinization. Inhibition of the Na+/H+ exchanger prevents both EGF as well as bombesin-induced alkalinization. However, under these conditions, bombesin evokes a rapid and sustained acidification related to the Ca2+ response. Apparently, bombesin initiates a Ca2+ -dependent acidifying process immediately after binding of the hormone to its receptor. Furthermore, we could demonstrate that the bombesin-induced alkalinization depends on protein kinase C activation whereas the EGF response does not. Determination of the total DG and InsPn accumulation revealed that EGF is ineffective in stimulating phospholipase C-mediated production of these second messengers. In contrast, bombesin causes a rapid DG and InsPn production coinciding with the Ca2+ response and the first phase of the rise in pHi followed by a slower DG accumulation coinciding with the second alkalinization phase. Our results show that in Swiss 3T3 cells the bombesin receptor activates the hydrolysis of inositol lipids as a mechanism of signal transduction, which consequently causes changes in Ca2+i and pHi. Clearly, the EGF receptor utilizes different pathways to evoke mitogenisis and stimulates Na+/H+ exchange independently of DG production and protein kinase C activation.
    Additional Material:
    5 Ill.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1002/jcp.1041420302

    Articles: DFG German National Licenses