Search Results - (Author, Cooperation:W. Marczewski)

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  1. 1
    Staff View
    Publication Date:
    2018-02-03
    Publisher:
    American Chemical Society (ACS)
    Print ISSN:
    0743-7463
    Electronic ISSN:
    1520-5827
    Topics:
    Chemistry and Pharmacology
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    Publication Date:
    2015-08-01
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Marczewski, W. ; Talarczyk, A. ; Hennig, J.

    Oxford, UK : Blackwell Publishing Ltd
    Published 2001
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    A random amplified polymorphic DNA marker OPG17450 linked to the Ns gene that confers resistance of potato to potato virus S (PVS), was used to develop sequence-characterized amplified region (SCAR) markers. After cloning and sequencing of OPG17450 new polymerase chain reaction (PCR) primers were designed to generate dominant (SCG17321) and codominant (SCG17448) markers. For SCG17448, polymorphism between susceptible and resistant genotypes was recovered after digestion of the marker with the restriction enzyme Muni. In addition to the band corresponding to ‘susceptible’ allele that does not contain the Muni cleavage site, two bands of approximately 251 bp and 197 bp were observed in the resistant genotypes. The usefulness of these SCAR markers was verified in diploid potatoes possessing the Ns locus from clone G-LKS 678147/60, and in tetraploid potatoes derived from G-LKS 678147/60 and from clone MPI 65118/3.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  4. 4
    Marczewski, W. ; Ostrowska, K. ; Zimnoch-Guzowska, E.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1998
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Using the RAPD method and bulked segregant analysis we identified four RAPD markers linked to a dominant gene Ns, responsible for a hypersensitive reaction of potato (Solanum tuberosum L.) to potato virus S (PVS) infection. The markers OPE15550, OPJ13500, OPG17450 and OPH19900 were found to be closely linked to the Ns gene in diploid potato clones. They are situated at 2.6, 3.3, 4.6 and 6.6 cM from Ns, respectively. As a source of the gene, clone G-LKS 678147/60, which is known to carry Ns transferred from S. tuberosum ssp. andigena was used. These RAPD markers were not amplified in resistant tetraploid clones containing Ns derived from the clone MPl65 118/3, also having an andigenum origin. This suggests that there may be two separate loci of Ns in the sources identified, or different alleles with the same specificity at a single locus, or that the genetic background of tetraploids tested results in different RAPD amphlification patterns.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses