Search Results - (Author, Cooperation:T. Togashi)

2015-02-20

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

2011-04-09

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

1432-1998

Springer Online Journal Archives 1860-2000

Medicine

Abstract We present a case of chronic granulomatous disease with an angiographically proven pseudo-sequestration of the lung. The patient was a 15-year-old boy who was admitted to the hospital with symptoms of fever, cough, hemoptysis and a subcutaneous abscess.Aspergillus fumigatus was isolated from the sputum and the abscess. During treatment, angiography demonstrated on anomalous blood supply to the right middle lobe. The therapeutic implications of pseudo-sequestration of the lung for the treatment of chronic granulomatous disease are discussed.

Electronic Resource

1432-0649

PACS: 42.60; 42.55; 42.79

Springer Online Journal Archives 1860-2000

Physics

Electronic Resource

1432-0649

PACS: 42.55-f; 42.55.Xi; 42.65.Re

Springer Online Journal Archives 1860-2000

Physics

Abstract. We have developed an all-solid-state Ti:sapphire laser system, which produces 22-fs, 0.2-TW pulses at 5-kHz repetition rate. An average power of 22.2 W is the highest ever obtained in ultrashort laser sources. The serious thermal lensing due to high power pumping in a small area of Ti:sapphire crystal is controlled successfully by a stable quasi-cavity with two concave mirrors. The attempt to increase the repetition rate to 10 kHz is also described.

Electronic Resource

1432-2145

Key words Evolution of anisogamy ; Gamete motility dimorphism ; Monostroma angicava ; Reproductive investment

Springer Online Journal Archives 1860-2000

Biology

Abstract  The reproductive strategy of a marine alga with a heteromorphic biphasic life cycle was studied by analyzing various sexual reproductive characters in light of the evolution of anisogamy. Gametophytes of Monostroma angicava were dioecious and their gametes were slightly anisogamous. Volume of gametangium, density of gametangia and area of mature gametangial parts on each gametophyte did not differ from male to female. Therefore, the reproductive biomass investment for gamete production was considered to be the same for each sex. Anisogamy in this alga appeared to be derived from the difference in the number of cell divisions during gametogenesis, because the majority of male gametangia each produced 64 (26) gametes and the female produced 32 (25) gametes. This corresponded with measurements of cell size in male and female gametes. Further, the sex ratio was 1:1 for sexually mature plants sampled at Charatsunai. Therefore, it was suggested that in the field twice as many male gametes are released as female gametes. Liberated gametes of both sexes showed positive phototaxis. The swimming velocity of freshly liberated male gametes was a little higher than that of female gametes. Male gametes had the potential to swim for ca. 72 h and female gametes for ca. 84 h. The difference in gamete motility between the two sexes seemed to be related to cell size. Planozygotes were negatively phototactic and swam more rapidly than gametes of either sex.

Electronic Resource

1432-2145

Key words  Evolution of anisogamy ; Mating behavior ; Mating efficiency ; Monostroma angicava ; Phototaxis

Springer Online Journal Archives 1860-2000

Biology

Abstract. The role of phototactic behavior of gametes was tested experimentally in the slightly anisogamous marine green alga Monostroma angicava Kjellman, and the effect of phototaxis on mating efficiency was discovered. Both male and female gametes showed positive phototaxis in response to a white light source. In contrast, they did not respond to a red light source. Their swimming velocity did not differ between these two illuminating light sources. It was, therefore, suggested that the search ability of the gamete itself might not vary between phototactic and non-phototactic conditions. The number of zygotes formed during the mating process may be expressed as the product of the number of encounters between male and female gametes and the fraction of encounters that result in sexual fusion. In this study, with high densities of male and female gametes mixed in test tubes, almost all minor (fewer in number) gametes fused sexually within 10 min. After dilution of the gamete suspensions by half, mating efficiency in test tubes illuminated by white light from above was higher than that in dark controls. This suggests that male and female gametes gathered at the water surface through their positive phototaxis, thus increasing the rate of encounters. Mating efficiency also decreased if the test tubes were illuminated from above by white light and also shaken. Since negative phototaxis is clearly shown in planozygotes, we suggest that positive phototaxis of male and female gametes in M. angicava is an adaptive trait for increasing the rate of gametic encounters rather than for the dispersal of zygotes as previously reported for zoospores of some marine algae.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary Newborn WKA rats given a single intraperitoneal injection of MMC treated TARS-1, a rat T cell line producing HTLV-I, were shown to accumulate high titred antibodies specific for HTLV-I. Adult WKA rats rejected TARS-1 transplant with transient appearance of anti-HTLV-I antibodies. However, rats maintained under daily administration of Bredinin, an immunosuppressive drug after TARS-1 transplant showed continuous production of antibodies specific for HTLV-I by aging. Type-C virus particles similar to HTLV-I were demonstrated by electronmicroscopy in the short-term cultured spleen cells of these rats. The evidence indicates that HTLV-I can be transmitted into newborn and immunosuppressed adult rats and they may provide a suitable animal model of ATL and related conditions in man, especially for elucidating the virus-host interactions involved in the leukemogenesis of HTLV-I. By using monoclonal antibodies, cell surface antigens associated with HTLV-I were also analysed.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary Mouse hybridoma cell lines were produced by fusion of P3 × 63 Ag8 myeloma cells with spleen cells from BALB/c mice immunized with purified measles virions. About 60 per cent of single cell colonies in wells were found to produce measles antibodies as determined by a radioimmune assay. Selected measles antibody producing hybridoma cell lines were passaged intraperitoneally in mice and ascites fluids were collected. This material contained 20–200 times higher antibody titers than unconcentrated medium from hybridoma cell lines propagated in tissue culture. The ascites fluid antibody products of 23 hybridoma cell lines were characterized by different measles serological tests. Seventeen lines produced high titers of hemagglutination inhibiting (HI) and hemolysis-inhibition (HLI) antibodies. One hybridoma cell line produced Ig with low HI but high HLI activity and the remaining 5 hybridoma cell line products only carried HLI activity. Unexpectedly it was found in radioimmune precipitation assays that all hybridomas studied, including those showing HLI but no HI antibody activity, gave a selective precipitation of the 79K measles hemagglutinin polypeptide. Radioimmune precipitation assays with sera from immunized animals showed that they contained high titers of antibodies precipitating the 79 K polypeptide but in addition also somewhat lower titers of antibodies precipitating the 60 K nucleoprotein, 40 K fusion and 36 K matrix polypeptides. Homogeneous Ig products carrying measles antibody activity were demonstrated by imprint immunoelectrophoresis of ascites materials.

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary Mouse hybridoma antibodies against 5 different structural components of measles virus were used in immune fluorescence tests to characterize the appearance of viral antigens in productively infected cell cultures. The antibodies employed in the tests reacted specifically with the hemagglutinin (H, 79K), polymerase (P, 72K), nucleocapsid (NP, 60K) hemolysin-fusion factor (F, 41+20K) and matrix (M, 36K) proteins. Syncytia formed in lytically infected cultures and single isolated cells in persistently infected cell cultures were both examined. Antibodies against NP and P proteins stained cytoplasmic inclusions varying in size from small dots to more confluent masses, frequently in a perinuclear position. Nuclei of infected cells contained exclusively NP antigen. Antibodies to envelope components—H, F and M—stained cytoplasmic membrane structures and also gave a granular cytoplasmic staining, especially in syncytia. Although all persistently infected cells produced NP antigen and the associated P component, they had a restricted capacity to produce demonstrable amount of envelope antigens. The occurrence of cells containing envelope antigens varied between about 50 and 5 per cent with H and F antigens giving the highest and lowest frequence values, respectively. It is proposed that a restricted capacity of cells to produce biologically active fusion protein is a prerequisite for maintaining a persistant infection in actively dividing cellsin vitro.

Electronic Resource