Search Results - (Author, Cooperation:T. Huynh)

2011-05-28

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Active Transport, Cell Nucleus ; Animals ; *Autophagy ; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors/genetics/*metabolism ; COS Cells ; Cell Nucleus/*metabolism ; Cells, Cultured ; Cercopithecus aethiops ; Cytoplasm/metabolism ; Gene Expression Regulation ; HeLa Cells ; Humans ; Liver/metabolism ; Lysosomes/*metabolism ; MAP Kinase Signaling System ; Mice ; Mice, Transgenic ; Microtubule-Associated Proteins/metabolism ; Mitogen-Activated Protein Kinase 1/metabolism ; Phagosomes/metabolism ; Phosphorylation ; RNA Interference ; Transcription, Genetic ; Up-Regulation

2014-11-20

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Alleles ; Animals ; Breast Neoplasms/*drug therapy/*genetics/metabolism/pathology ; Drug Resistance, Neoplasm/drug effects/*genetics ; Female ; Humans ; Loss of Heterozygosity/drug effects/genetics ; Mice ; Mice, Nude ; PTEN Phosphohydrolase/*deficiency/*genetics/metabolism ; Phosphatidylinositol 3-Kinases/*antagonists & inhibitors ; Thiazoles/*pharmacology/therapeutic use ; Xenograft Model Antitumor Assays

2018-11-01

American Chemical Society (ACS)

0002-7863

1520-5126

Chemistry and Pharmacology

2018-04-11

Institute of Physics (IOP)

1757-8981

1757-899X

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

2018-07-17

The American Association for Cancer Research (AACR)

1078-0432

1557-3265

Medicine

1365-3083

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

TRIM is a recently identified transmembrane adaptor protein which is exclusively expressed in T cells and natural killer (NK) cells. In peripheral blood T cells TRIM has been reported to coprecipitate, comodulate, and cocap with the T-cell receptor (TCR), suggesting that it is an integral component of the TCR/CD3/ζ complex. Here we investigate the expression of TRIM mRNAs and proteins in developing thymocytes. Two splicing isoforms with open reading frames are observed, namely a full length (TRIM) and a truncated version (ΔTM-TRIM). The latter lacks the extracellular and transmembrane domains as well as the first 10 cytoplasmic aminoacids and is significantly expressed only as mRNA in early fetal thymocytes. TRIM mRNA is detected in all mainstream thymocyte subsets in adult mice. TRIM protein, in contrast, first appears in the DN2 (CD44+ CD25+) subset of adult double negative (DN) cells. In fetal thymocyte development, TRIM mRNA is seen from dg 14.5 onwards whereas TRIM protein appears first on dg 16.5. In contrast to the adult, the TRIM protein was seen in a subset of fetal DN1 cells. In fetal and adult thymocytes, TRIM protein expression was highest in DN2, DN3 (CD44−25+) and in DP cells, compatible with a functional role at or around phases of thymic selection.

Electronic Resource

1365-2222

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Background Chemokine receptors (CR) play an important role in T cell migration, but their contribution to lung trafficking is unclear.Objective We hypothesized that if a particular CR was involved in T cell homing its expression would be enriched on lung T cells compared with peripheral blood T cells (PBT).Methods We have measured the CR expression on BAL T cells from patients with sarcoid, other interstitial lung diseases (ILD), asthma and healthy volunteers.Results Of 14 CR studied in sarcoid, CXCR6 expression was the most markedly increased in the lung compared with the blood, a finding that was also seen in ILD patients. A striking although lesser increase was also seen in asthmatics and healthy controls. Analysis of expression of the CXCR6 ligand, CXCL16, by immunohistochemistry suggested that alveolar macrophages (AM) were the major source of CXCL16 in the lung. AM expressed mRNA for CXCL16 and released nanogram quantities after adhesion to plastic as shown by RT-PCR, Western blotting and ELISA. Bronchoalveolar lavage (BAL) fluid from all subjects contained large amounts of CXCL16. The full-length CXCL16 was the predominant isoform in AM lysates, supernatants and BAL.Conclusion This data suggests that CXCR6 and CXCL16 may play a role in T cell recruitment to the lung.

Electronic Resource

0378-1119

RNA splicing ; Recombinant DNA ; T lymphocytes ; genomic cloning ; phage λ vectors ; protein tyrosine kinase

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

0021-9673

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Chemistry and Pharmacology

Electronic Resource

0014-5793

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

1573-0530

Springer Online Journal Archives 1860-2000

Mathematics

Physics

Abstract The method of *-polarization connects phase space mechanics to the usual operator formulation of quantum theory. A *-polarization is a linear submanifold of the space of C ∞ functions on phase space. Elements of a *-polarization are in direct correspondence with the Schroedinger wave functions and this correspondence induces the Weyl correspondence between classical observables and operators. All generalized Moyal algebras admit *-polarizations and a general method is thus available for translating *-quantization into operator language.

Electronic Resource

1432-2242

Agrocybe aegerita ; Genetic variability ; Incompatibility genes ; Allelic series

Springer Online Journal Archives 1860-2000

Biology

Summary The variability of the sexual incompatibility genes of Agrocybe aegerita was investigated in the homokaryotic progeny of 13 wild dikaryotic strains from five distinct European geographic origins. Results of mating tests allowed identification of 18 A alleles and 16 B alleles out of a possible 26 different alleles for each in the sample. The determination and the comparison by a contingency χ 2 test of the frequencies of allele replications between intra- and interregional matings showed no departure from a random distribution of incompatibility alleles. The allelic series estimated for the incompatibility genes of the entire population of A. aegerita, 30 A and 25 B aleles, are significantly less extensive than those already hypothesized for other tetrapolar hymenomycetes. However, the low variability of incompatibility genes has little effect on the outbreeding efficiency (92.6%) of this mushroom. The low variability of the incompatibility alleles and the apparent absence of intrafactorial recombination could relate to a single-locus structure of the incompatibility genes in A. aegerita.

Electronic Resource

1573-4803

Springer Online Journal Archives 1860-2000

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Abstract The thermomechanical durability of a two-dimensionally woven and a three-dimensionally braided SiC/SiC composite fabricated by chemical vapour infiltration technique were studied. The effects of thermal shock, thermal ageing, and thermal cycling at high temperatures on mechanical properties were determined. The thermal degradation mechanisms were also investigated. The results indicated that the fibre geometry, matrix porosity, interlaminar shear strength and testing conditions affect the thermomechanical durability of the SiC/SiC composites.

Electronic Resource

0021-9541

Life and Medical Sciences ; Cell & Developmental Biology

Wiley InterScience Backfile Collection 1832-2000

Biology

Medicine

Retinoids are potent inhibitors of growth and tumor progression in many mammary carcinoma cell lines, though regulation of growth in nontumorigenic mammary epithelial cells by retinoids is less clear. Here, we have characterized the inhibition of MAC-T (a nontransformed bovine mammary epithelial cell line) cellular proliferation by retinoids and their role in regulating insulin-like growth factor binding proteins (IGFBPs). Retinoic acid (RA) (100 nM) was a potent inhibitor of MAC-T cell proliferation. Retinol was 10-100 times less effective. Neither retinoid could completely arrest growth at noncytotoxic concentrations. Retinoic acid inhibited cellular proliferation by 1 h (P 〈 .05), but inhibition was fivefold greater by 24 h (P 〈 .01). This second stage of growth inhibition (after 12 h) was dependent upon protein synthesis. However, RA-induced inhibition of cellular proliferation did not persist, with thymidine incorporation increasing toward control levels by 4 days in culture. Retinoic acid was less effective in inhibiting thymidine incorporation when cells were stimulated with insulin, des(1-3) IGF-I, or Long(R3) IGF-I when compared to cells stimulated with native IGF-I or serum. Inhibition of proliferation by RA was associated with increased levels of IGFBP-2 in conditioned media and in plasma membrane preparations. Treatment with insulin or des(1-3) IGF-I resulted in the appearance of IGFBP-3 in conditioned media and on the cell surface. However, RA significantly reduced IGFBP-3 levels in conditioned media and eliminated IGFBP-3 associated with the plasma membrane. Thus, RA is a potent but transient inhibitor of bovine mammary epithelial cell proliferation, and this growth inhibition is correlated with increased IGFBP-2 accumulation and inhibition of IGF-I stimulated IGFBP-3 protein secretion. © 1996 Wiley-Liss, Inc.

9 Ill.

Electronic Resource