Search Results - (Author, Cooperation:T. Decker)

2018-06-26

American Physical Society (APS)

0031-9007

1079-7114

Physics

Condensed Matter: Structure, etc.

2013-04-27

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Animals ; Caspase 1 ; Coinfection/*immunology/pathology ; Disease Models, Animal ; Host-Pathogen Interactions/immunology ; Interleukin-1beta/metabolism ; *Legionella pneumophila ; Legionnaires' Disease/*immunology/pathology ; Lung/microbiology/pathology/virology ; Mice ; Mice, Inbred C57BL ; *Orthomyxoviridae ; Orthomyxoviridae Infections/*immunology/pathology ; Pneumonia, Bacterial/*immunology/pathology ; Toll-Like Receptor 2/metabolism ; Toll-Like Receptor 3/metabolism ; Toll-Like Receptor 4/metabolism ; Tumor Necrosis Factor-alpha/metabolism

2018-08-17

American Society of Hematology (ASH)

0006-4971

1528-0020

Biology

Medicine

Free Research Articles, Myeloid Neoplasia, Lymphoid Neoplasia, Clinical Trials and Observations

2018-09-25

American Physical Society (APS)

0031-9007

1079-7114

Physics

Condensed Matter: Electronic Properties, etc.

1089-7690

AIP Digital Archive

Physics

Chemistry and Pharmacology

We report on the shear modulus G of colloidal crystals formed from thoroughly deionized suspensions of charged latex spheres. G is measured as a function of particle number density n. Body- and face-centered-cubic (bcc and fcc) crystal structures are observed by simultaneously performed static light scattering, and a broad coexistence region is found between (2.7±0.1)×1018 m−3≤n≤(4.8±0.2)×1018 m−3. Below n=1019 m−3, G closely follows theoretical predictions for both bcc and fcc, while it stays constant throughout the transition region. Above n=1019 m−3, G still observes the predicted n-dependence but with values larger than expected. While in that region, an upper bound of particles per crystallite is estimated from scattering data to be on the order of 104; the abrupt change in G cannot be solely attributed to the gradual morphological transition from polycrystalline to nanocrystalline materials. © 1998 American Institute of Physics.

Electronic Resource

1365-2559

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Aims:This study was performed to determine the diagnostic value of keratin 5/6 (CK 5/6) immunophenotyping on routinely processed breast tissues.Methods and results:Six hundred and ninety-nine breast lesions, including normal tissues as well as benign and malignant lesions in 321 formalin-fixed, paraffin-embedded samples from 158 different patients were investigated immunohistochemically, following wet autoclave pre-treatment for antigen retrieval. In normal breast tissues, both myoepithelial and luminal epithelial cells expressed CK 5/6 in varying amounts. While myoepithelial immunoreactivity was most pronounced in the duct system, luminal epithelial immunoreactivity was strongest in the terminal duct lobular units. In ductal hyperplasias (DH), luminal epithelial cells predominantly revealed CK 5/6 immunoreaction. In contrast, neoplastic epithelial cells in atypical ductal and lobular hyperplasias (ADH and ALH) lacked such an expression, whereas in ductal in-situ carcinomas (DCIS) and in infiltrating ductal carcinomas 3.7% and 7.7%, of the cases respectively, showed positive immunostaining for CK 5/6.Conclusions:Immunophenotyping of keratin 5/6 expression can be helpful in the diagnosis of atypical hyperplasias and in-situ carcinomas of the breast. It is particularly valuable in the differential diagnosis between benign and atypical proliferative lesions.

Electronic Resource

0378-1119

GBP3 ; Genomic clones ; exon ; gene expression ; guanylate-binding protein ; intron

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

1433-044X

Key words Patella dislocation • Elsmlie-Trillat-procedure • Chondromalacia patellae ; Schlüsselwörter Patelladislokation • Elmslie-Trillat-Operation • Chondromalazia patellae

Springer Online Journal Archives 1860-2000

Medicine

Zusammenfassung Im Zeitraum von Mai 1981 bis Dezember 1993 wurden 120 Patienten (126 Kniegelenke) nach der Methode von Elmslie-Trillat wegen Patella(sub)luxation operiert; 79 Patienten (91 Kniegelenke, 72,2 %) wurden nach einem durchschnittlichen postoperativen Intervall von 4,1 (1–13,6) Jahren kontrolliert. Dabei handelte es sich um 46 Frauen und 33 Männer mit einem Durchschnittsalter bei Operation von 26,9 (14–59) Jahren. Wesentliche Kriterien der Auswertung waren der Score nach Bentley, radiologische Befunde (Patelladezentrierung, Patella-Tiefen-Index), der Chondromalziegrad und Patientencharakteristika (Alter, präoperatives Intervall u. a.); 64 Kniegelenke (70,3 %) wurden im Bentley-Schema mit „sehr gut“ und „gut“ bewertet. Signifikant schlechtere Ergebnisse wurden bei höhergradigen Knorpeldefekten (p 〈 0,0036), längerer präoperativer Anamnesedauer (p 〈 0,012) und älteren Patienten (p 〈 0,0023) gefunden. Schlechtere Ergebnisse zeigten sich auch bei Patienten mit voroperierten Kniegelenken (p 〈 0,0001). Gute Ergebnisse korrelierten mit adäquatem postoperativem Patellalauf (p 〈 0,001). Es zeigten sich hierbei keine Unterschiede in den Gruppen mit Patellaluxation und/oder Patellalateralisation. Den Ergebnissen folgend, sollte die Operation nach Elmslie-Trillat „gut und rechtzeitig“ durchgeführt werden. Eine Optimierung der Behandlungsstrategie bei einer Vielzahl bekannter Operationsmethoden bei Patelladezentrierung erscheint möglich, wenn die Verfahren risikogruppenbezogen analysiert und angewendet werden.

Summary Between May 1981 and December 1993, 120 patients (126 knee joints) were operated using the Elsmlie-Trillat procedure because of patella(sub)luxation. 79 patients were reviewed at an average follow up of 4.1 (1–13.6) years. 46 women and 33 men were operated with an average age at operation of 26.9 (14–59) years. The examination included Bentley’s Score, radiological parameters (e. g. grading of patella dislocation), grading of chondromalacia and patient characteristics (e. g. age, pre-operative time). 64 knee joints (70.3 %) were evaluated ’very good' and ’good', using the score of Bentley. There were significantly worse results in cases with higher grading of chondromalacia (p 〈0.0036), longer pre-operative time (p 〈 0.012) and older patients (p 〈 0.0023). The worst and most highly significant results were in these patients with prior knee surgery (p 〈 0.0001). A good patella relocation correlated with improved results (p 〈 0.001). There was no difference in cases with patella dislocation and/or patellasubluxation. In conclusion, the Elmslie-Trillat procedure should be carried out ’in the correct manner and at an early stage'. To optimise the therapy in cases of patella dislocation the high number of operation techniques should be analysed and used regarding risk groups.

Electronic Resource

1420-9071

Springer Online Journal Archives 1860-2000

Biology

Medicine

Electronic Resource

1420-9071

Key words. STAT; transcription; phosphorylation; gene; promoter.

Springer Online Journal Archives 1860-2000

Biology

Medicine

Abstract. The seven mammalian members of the signal transducer and activator of transcription (STAT) family share a common core structure which reflects their shared mechanism of activation, dimerization, and DNA binding. By contrast, the STAT C termini containing the sequences required for transcriptional activation are much less homologous, suggesting different ways by which individual STATs activate their target genes. This paper describes several important discoveries linked to mechanistic aspects of STAT transcription factor function. These include regulated serine phosphorylation of the transactivating domain, promoter-dependent interactions of STATs with each other, or of STATs with other transcription factors, and with transcriptional co-activators. The basis, background, and implications of these molecular events will be summarized and discussed.

Electronic Resource

1432-1963

Schlüsselwörter Duktales Carcinoma in situ ; Atypische duktale Hyperplasie ; Klassifikation ; Differentialdiagnose ; Key words Ductal carcinoma in situ ; Atypical Ductal hyperplasia ; Classification ; differential diagnosis

Springer Online Journal Archives 1860-2000

Medicine

Summary This review emphazises the pathology of premalignant ductal breast diseases and its practical relevance to the patients management. The histological criteria for recognizing Ductal Hyperplasia (DH) are now well established. These include an intraluminal heterogenous proliferation of glandular cells positive for keratins 8/18/19 and epithelial cells with expression of keratins 5/6/14. As a hyperplastic process the epithelial cells disclose an haphazard irregular growth with slit like irregular lumina (fenestrated growth pattern). The florid DH indicates a slight subsequent increased risk for cancer development. Our knowledge of the nature of noninvasive ductal neoplasia continues to evolve. Recent molecular genetic and immunohistochemical efforts have disclosed that atypical ductal hyperplasia (ADH) constituted a clonal neoplastic proliferation of an epithelial cell. Histological hallmarks of ADH are their cytologic features of uniformity and monotony of proliferation of cells and its micropapillary, cibriform or solid growth pattern. So from histology ADH simulates the highly differentiated DCIS, but can be distinguished from the latter quantitavely by the aggregate cross sectional diameter or the number of ducts that are completely involved by the atypical proliferation. ADH indicates a few fold subsequent increased risk for developing carcinoma. So this leson requires a close follow up with 3 to 4 examinations per year and annual mammograms. Ductal carcinoma in situ (DCIS) consists of cytologically malignant cells in the parenchyma that have not invaded into the stroma. Recent studies have shown that DCIS is a heterogenous group of tumors. Attempts have been made to classify it into histologic patterns, nuclear grades, tumors with or without comedo-necroses etc. We can draw the conclusion from several studies that the most important histologic feature is the nuclear grade. Holland et al. have suggested a very useful classification scheme that includes nuclear grade and histological features. The modifiers of treatment are as follows: 1. nuclear grade or differentiation of the DCIS 2. extention of the lesion 3. excision with clear margins So efforts to classify DCIS underscore the central role of pathology in determining the grade of the DCIS, its size and the adequacy of the surgical excision in terms of free margins.All three parameters are included in a score system of the Van Nuys Prognostic Index.

Zusammenfassung Die vorliegende Übersichtsarbeit beschäftigt sich mit der Differentialdiagnose der duktalen Hyperplasie und der Gruppe der nicht-invasiven duktalen Neoplasien. Die Duktale Hyperplasie (DH) ist durch eine intraduktale, heterogene bunte Zellproliferation aus proliferierenden Drüsenepithelien mit Expression luminaler Keratin und Epithelzellen vom basalen Keratintyp gekennzeichnet. In 10 % kommen zusätzlich apokrine Metaplasien vor. Das Myoepithel spielt keine oder nur eine untergeordnete Rolle. Die gegenüber normalem Brustdrüsengewebe erhöhte Proliferationsrate könnte die Erklärung für ein leicht erhöhtes Karzinomrisiko der Patientengruppe mit diesen Läsionen sein. Die atypische duktale Hyperplasie (ADH) stellt eine klonale Epithelproliferation dar. Nach überwiegender Meinung ist sie kein „Übergangsstadium“ von der duktalen Hyperplasie zum duktalen Carcinoma in situ (DCIS), sondern wird in einer Entwicklungslinie mit dem DCIS gesehen. So finden sich bei der ADH die gleichen zytologischen und architektonischen Kriterien wie beim hoch- und intermediär differenzierten DCIS. Die Unterscheidung zwischen diesen Läsionen erfolgt quantitativ. Das Duktale Carcinoma in situ (DCIS) ist eine maligne intraduktale Neoplasie der Mamma. Der DCIS-Begriff umfaßt eine Gruppe von histologisch differenten Läsionen mit Unterschieden im klinischen Bild, Mammographiebefund, intramammärer Ausdehnung und Verteilung sowie biologischen Markern. Darüber hinaus haben klinische Studien gezeigt, daß diese Läsionen sich auch in der Rezidivneigung und der Wahrscheinlichkeit der Progression zum invasiven Mammakarzinom unterscheiden.

Electronic Resource

1432-1963

Schlüsselwörter Mammakarzinom ; Brusterhaltende Therapie ; Exzisionsbiopsie ; Pathologische Untersuchung ; Key words Breast cancer ; Breast conserving therapy ; Excision biopsy ; Pathological examination

Springer Online Journal Archives 1860-2000

Medicine

Summary The long-term disease free survival in patients treated by breast conserving therapy is similar to that of patients treated by mastectomy. In breast conserving therapie there is a significant risk of local recurrence. Although local recurrence does not appear to effect survival, there is certainly associated morbidity and attendant emotional trauma. Microscopic evaluation of the margins of lumpectomy specimens is the only way to define the extent of the tumour and the adequacy of resection. We intended to check the influence of standardized handling instructions for pathological examination on

Zusammenfassung Die Langzeitüberlebenszeit von Patienten nach brusterhaltender Therapie (BET) des Mammakarzinoms ist vergleichbar der nach Mastektomie. Bei der BET besteht ein signifikantes Rezidivrisiko. Obwohl das Rezidiv die Überlebenszeit nicht alteriert, ist eine erhebliche Morbidität und meist ein schweres psychisches Trauma damit verbunden. Die histologische Untersuchung der Resektionränder des BET-Präparats ist der einzige Weg um die Tumorausdehnung zu bestimmen und die Radikalität des Eingriffs zu bemessen. Wir untersuchten den Einfluß eines Standards für die pathologische Untersuchung auf die histologischen RR-Befunde. Der Untersuchungsstandard ist Teil eines interdiziplinären Praxisprotokolls zur BET. Wir verglichen die Ergebnise unserer standardisierten Untersuchung anhand von 100 Fällen mit den RR-Befunden von je 100 Fällen aus Phasen mit unterschiedlichem Untersuchungsgang: Vor 1989 wurden nur auf Tumor verdächtige RR histologisch untersucht; 1990 und 1991 wurden die RR intensiver aber ohne Bezug zum Verlauf des Gangsystems untersucht, was 1992 eingeführt wurde. In 28 % d. F. fand sich histologisch Tumor in einem oder mehreren makroskopisch unauffälligen RR. In den beiden anderen Perioden wurde nur in 2 % bzw. 12 % d. Fälle Tumor im RR entdeckt. Diese Ergebnisse belegen einen wesentlichen Einfluß unseres Standards auf die Untersuchungsergebnisse der Exzisate: Erstens besteht eine höhere Sensitivität für tumorhaltige RR im Vergleich mit sporadischem Untersuchungsgang; darüberhinaus besteht auf der Basis der duktalen Orientierung des Standards ein bessere Möglichkeit zur Aussage über die intraduktale Tumorkomponente. Schließlich kann die Lokalisation tumorhaltiger RR genau angegeben werden. Durch Anwendung des Untersuchungsstandards sind wir somit in der Lage, zur Qualitätssicherung der BET beizutragen.

Electronic Resource

0021-9541

Life and Medical Sciences ; Cell & Developmental Biology

Wiley InterScience Backfile Collection 1832-2000

Biology

Medicine

The malignant histiocytosis sarcoma virus (MHSV) contains Ha-v-ras-related oncogenic sequences and rapidly transforms myeloid cells in vivo and in vitro. Myeloid cell lines can be derived which do not require growth factor for continued proliferation. We initiated this work to define the process of transformation leading to autonomy of cell growth in transformed myeloid cells. Five established cell lines were examined. All express macrophagespecific cell-surface antigens and exhibit several other properties typical for mature macrophages. Growth properties, growth factor release, and growth factor receptor presentation were examined: Release of growth factors is not a consistent feature. All cell lines show cell-density-independent colony formation and do not release self-stimulating factors, thus excluding autocrine stimulation as a model leading to transformation. All cell lines express unusually high levels of granulocyte-macrophage (GM)-and multi-CSF receptors and, except for one M-CSF receptors. The high increase in GM-CSF and other growth factor receptors may be causally related to the transformed state of the cells. MHSV can be used as a tool to easily derive cell lines of the macrophage pathway as a model to study myeloid transformation, differentiation, and macrophage function.

2 Ill.

Electronic Resource