Search Results - (Author, Cooperation:T. Arakawa)
-
1Latest Papers from Table of Contents or Articles in PressT. Arakawa ; T. Kobayashi-Yurugi ; Y. Alguel ; H. Iwanari ; H. Hatae ; M. Iwata ; Y. Abe ; T. Hino ; C. Ikeda-Suno ; H. Kuma ; D. Kang ; T. Murata ; T. Hamakubo ; A. D. Cameron ; T. Kobayashi ; N. Hamasaki ; S. Iwata
American Association for the Advancement of Science (AAAS)
Published 2015Staff ViewPublication Date: 2015-11-07Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Anion Exchange Protein 1, Erythrocyte/*chemistry/genetics ; Crystallography, X-Ray ; Disease/genetics ; Escherichia coli Proteins/chemistry ; Humans ; Membrane Transport Proteins/chemistry ; Mutation ; Protein Structure, Secondary ; Protein Structure, TertiaryPublished by: -
2Latest Papers from Table of Contents or Articles in PressA. R. Forrest ; H. Kawaji ; M. Rehli ; J. K. Baillie ; M. J. de Hoon ; V. Haberle ; T. Lassmann ; I. V. Kulakovskiy ; M. Lizio ; M. Itoh ; R. Andersson ; C. J. Mungall ; T. F. Meehan ; S. Schmeier ; N. Bertin ; M. Jorgensen ; E. Dimont ; E. Arner ; C. Schmidl ; U. Schaefer ; Y. A. Medvedeva ; C. Plessy ; M. Vitezic ; J. Severin ; C. Semple ; Y. Ishizu ; R. S. Young ; M. Francescatto ; I. Alam ; D. Albanese ; G. M. Altschuler ; T. Arakawa ; J. A. Archer ; P. Arner ; M. Babina ; S. Rennie ; P. J. Balwierz ; A. G. Beckhouse ; S. Pradhan-Bhatt ; J. A. Blake ; A. Blumenthal ; B. Bodega ; A. Bonetti ; J. Briggs ; F. Brombacher ; A. M. Burroughs ; A. Califano ; C. V. Cannistraci ; D. Carbajo ; Y. Chen ; M. Chierici ; Y. Ciani ; H. C. Clevers ; E. Dalla ; C. A. Davis ; M. Detmar ; A. D. Diehl ; T. Dohi ; F. Drablos ; A. S. Edge ; M. Edinger ; K. Ekwall ; M. Endoh ; H. Enomoto ; M. Fagiolini ; L. Fairbairn ; H. Fang ; M. C. Farach-Carson ; G. J. Faulkner ; A. V. Favorov ; M. E. Fisher ; M. C. Frith ; R. Fujita ; S. Fukuda ; C. Furlanello ; M. Furino ; J. Furusawa ; T. B. Geijtenbeek ; A. P. Gibson ; T. Gingeras ; D. Goldowitz ; J. Gough ; S. Guhl ; R. Guler ; S. Gustincich ; T. J. Ha ; M. Hamaguchi ; M. Hara ; M. Harbers ; J. Harshbarger ; A. Hasegawa ; Y. Hasegawa ; T. Hashimoto ; M. Herlyn ; K. J. Hitchens ; S. J. Ho Sui ; O. M. Hofmann ; I. Hoof ; F. Hori ; L. Huminiecki ; K. Iida ; T. Ikawa ; B. R. Jankovic ; H. Jia ; A. Joshi ; G. Jurman ; B. Kaczkowski ; C. Kai ; K. Kaida ; A. Kaiho ; K. Kajiyama ; M. Kanamori-Katayama ; A. S. Kasianov ; T. Kasukawa ; S. Katayama ; S. Kato ; S. Kawaguchi ; H. Kawamoto ; Y. I. Kawamura ; T. Kawashima ; J. S. Kempfle ; T. J. Kenna ; J. Kere ; L. M. Khachigian ; T. Kitamura ; S. P. Klinken ; A. J. Knox ; M. Kojima ; S. Kojima ; N. Kondo ; H. Koseki ; S. Koyasu ; S. Krampitz ; A. Kubosaki ; A. T. Kwon ; J. F. Laros ; W. Lee ; A. Lennartsson ; K. Li ; B. Lilje ; L. Lipovich ; A. Mackay-Sim ; R. Manabe ; J. C. Mar ; B. Marchand ; A. Mathelier ; N. Mejhert ; A. Meynert ; Y. Mizuno ; D. A. de Lima Morais ; H. Morikawa ; M. Morimoto ; K. Moro ; E. Motakis ; H. Motohashi ; C. L. Mummery ; M. Murata ; S. Nagao-Sato ; Y. Nakachi ; F. Nakahara ; T. Nakamura ; Y. Nakamura ; K. Nakazato ; E. van Nimwegen ; N. Ninomiya ; H. Nishiyori ; S. Noma ; T. Noazaki ; S. Ogishima ; N. Ohkura ; H. Ohimiya ; H. Ohno ; M. Ohshima ; M. Okada-Hatakeyama ; Y. Okazaki ; V. Orlando ; D. A. Ovchinnikov ; A. Pain ; R. Passier ; M. Patrikakis ; H. Persson ; S. Piazza ; J. G. Prendergast ; O. J. Rackham ; J. A. Ramilowski ; M. Rashid ; T. Ravasi ; P. Rizzu ; M. Roncador ; S. Roy ; M. B. Rye ; E. Saijyo ; A. Sajantila ; A. Saka ; S. Sakaguchi ; M. Sakai ; H. Sato ; S. Savvi ; A. Saxena ; C. Schneider ; E. A. Schultes ; G. G. Schulze-Tanzil ; A. Schwegmann ; T. Sengstag ; G. Sheng ; H. Shimoji ; Y. Shimoni ; J. W. Shin ; C. Simon ; D. Sugiyama ; T. Sugiyama ; M. Suzuki ; N. Suzuki ; R. K. Swoboda ; P. A. t Hoen ; M. Tagami ; N. Takahashi ; J. Takai ; H. Tanaka ; H. Tatsukawa ; Z. Tatum ; M. Thompson ; H. Toyodo ; T. Toyoda ; E. Valen ; M. van de Wetering ; L. M. van den Berg ; R. Verado ; D. Vijayan ; I. E. Vorontsov ; W. W. Wasserman ; S. Watanabe ; C. A. Wells ; L. N. Winteringham ; E. Wolvetang ; E. J. Wood ; Y. Yamaguchi ; M. Yamamoto ; M. Yoneda ; Y. Yonekura ; S. Yoshida ; S. E. Zabierowski ; P. G. Zhang ; X. Zhao ; S. Zucchelli ; K. M. Summers ; H. Suzuki ; C. O. Daub ; J. Kawai ; P. Heutink ; W. Hide ; T. C. Freeman ; B. Lenhard ; V. B. Bajic ; M. S. Taylor ; V. J. Makeev ; A. Sandelin ; D. A. Hume ; P. Carninci ; Y. Hayashizaki
Nature Publishing Group (NPG)
Published 2014Staff ViewPublication Date: 2014-03-29Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; *Atlases as Topic ; Cell Line ; Cells, Cultured ; Cluster Analysis ; Conserved Sequence/genetics ; Gene Expression Regulation/genetics ; Gene Regulatory Networks/genetics ; Genes, Essential/genetics ; Genome/genetics ; Humans ; Mice ; *Molecular Sequence Annotation ; Open Reading Frames/genetics ; Organ Specificity ; Promoter Regions, Genetic/*genetics ; RNA, Messenger/analysis/genetics ; Transcription Factors/metabolism ; Transcription Initiation Site ; Transcription, Genetic/genetics ; Transcriptome/*geneticsPublished by: -
3Latest Papers from Table of Contents or Articles in PressN. Nomura ; G. Verdon ; H. J. Kang ; T. Shimamura ; Y. Nomura ; Y. Sonoda ; S. A. Hussien ; A. A. Qureshi ; M. Coincon ; Y. Sato ; H. Abe ; Y. Nakada-Nakura ; T. Hino ; T. Arakawa ; O. Kusano-Arai ; H. Iwanari ; T. Murata ; T. Kobayashi ; T. Hamakubo ; M. Kasahara ; S. Iwata ; D. Drew
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-09-30Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsPublished by: -
4Latest Papers from Table of Contents or Articles in PressT. Hino ; T. Arakawa ; H. Iwanari ; T. Yurugi-Kobayashi ; C. Ikeda-Suno ; Y. Nakada-Nakura ; O. Kusano-Arai ; S. Weyand ; T. Shimamura ; N. Nomura ; A. D. Cameron ; T. Kobayashi ; T. Hamakubo ; S. Iwata ; T. Murata
Nature Publishing Group (NPG)
Published 2012Staff ViewPublication Date: 2012-01-31Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Allosteric Regulation/*drug effects ; Animals ; Antibodies, Monoclonal/immunology/*pharmacology ; Complementarity Determining Regions/immunology ; *Drug Inverse Agonism ; Humans ; Immunoglobulin Fab Fragments/immunology/pharmacology ; Ligands ; Mice ; Models, Molecular ; Opsins/immunology ; Pichia ; Protein Conformation/drug effects ; Receptor, Adenosine A2A/chemistry/immunology/*metabolism ; Receptors, G-Protein-Coupled/agonists/*antagonists & ; inhibitors/chemistry/*immunologyPublished by: -
5Articles: DFG German National LicensesArakawa, T. ; Nishioka, M. ; Nagamune, Y. ; Arakawa, Y.
Woodbury, NY : American Institute of Physics (AIP)
Published 1994Staff ViewISSN: 1077-3118Source: AIP Digital ArchiveTopics: PhysicsNotes: A strained InGaAs quantum wire laser with a vertical microcavity structure was fabricated for the first time. In this laser structure, quantum wires with a lateral width of about 10 nm were grown by a selective metalorganic chemical vapor deposition technique. The length of the microcavity was 4λ(λ=883 nm), with AlAs/AlGaAs distributed Bragg reflectors. The cavity effect was demonstrated by the measurement of photoluminescence with and without the cavity. Lasing oscillation was observed at 77 K by optical pumping.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesArakawa, T. ; Watabe, H. ; Nagamune, Y. ; Arakawa, Y.
Woodbury, NY : American Institute of Physics (AIP)
Published 1996Staff ViewISSN: 1077-3118Source: AIP Digital ArchiveTopics: PhysicsNotes: We have fabricated ridge-type InGaAs quantum wire structures on a (110) cleaved plane of AlGaAs/GaAs superlattice using selective metalorganic chemical vapor deposition growth. The lateral width of the quantum wires was about 25 nm. Spatially resolved photoluminescence (PL) of the quantum wires was observed by using microscopic photoluminescence measurement at low temperature, showing PL from the quantum wire region and its polarization dependence. © 1996 American Institute of Physics.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesArakawa, T. ; Kato, Y. ; Sogawa, F. ; Arakawa, Y.
Woodbury, NY : American Institute of Physics (AIP)
Published 1997Staff ViewISSN: 1077-3118Source: AIP Digital ArchiveTopics: PhysicsNotes: We investigated the quantum confined Stark effect in GaAs quantum wires formed in a V-groove structure, demonstrating observation of a blueshift of the photoluminescence peak with the increase of electric fields at 50 K. This blueshift is attributed to the fact that the change in enhanced binding energy of excitons due to the electric field is larger than that in quantized energy levels of electrons and holes. Time-resolved photoluminescence was also measured. The photoluminescence decay time is decreased in small quantum wires of 8 nm width with the increase of electric fields, while the decay time is increased in the quantum wires with a size of 35 nm. These results indicate that the escaping of carriers is more dominant in smaller structures than reduction of the oscillator strength due to the electric fields. © 1997 American Institute of Physics.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesNIEWOLD, Th. A. ; GRUYS, E. ; ARAKAWA, T. ; SHIRAHAMA, T. ; KISILEVSKY, R.
Oxford, UK : Blackwell Publishing Ltd
Published 1993Staff ViewISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Tumour necrosis factor-α (TNF-α) is one of the cytokines that stimulate the production of serum amyloid A (SAA), the precursor of AA amyloid. The role of TNF-α in amyloidogenesis was investigated in experimental hamsters using purified recombinant human TNF-α (rhTNF-α) and rhTNF-α analogue different from the normal molecule by two amino acid substitutions. Daily injections of 1 μg rhTNF-α resulted in elevated SAA levels but even in the presence of amyloid enhancing factor (AEF) no amyloid was deposited, indicating that apart from the AEF and one particular SAA stimulating factor an additional factor is needed to result in amyloid deposition. This factor is generated by repeated injections of E. coli lipopolysaccharide (LPS).A single intraperitoneal injection of 12,5 μg or more of rhTNF-ä followed by seven daily subcutaneous injections of LPS resulted in enhanced amyloid deposition. Heat denaturation of rhTNF-α did abolish its AEF activity. The rhTNF-α analogue, having one-fifth of the cytotoxic activity of the normal rhTNF-α, showed a similar reduction in its SAA-inducing capacity and its amyloidogenicity. This suggests the AEF activity to be closely related to TNF-α activity. However, poly(I)poly(C) (a potent inducer of IL-6) also showed AEF activity, suggesting that not a single cytokine but rather a certain combination of different cytokines could be decisive in AA amyloidogenesis.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesYokoyama, K. ; Ohtsuka, T. ; Kuraishi, C. ; Ono, K. ; Kita, Y. ; Arakawa, T. ; Ejima, D.
Oxford, UK : Blackwell Publishing Ltd
Published 2003Staff ViewISSN: 1750-3841Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionProcess Engineering, Biotechnology, Nutrition TechnologyNotes: : The recombinant microbial transglutaminase from Streptoverticillium mobaraense var. (rMTGase) was expressed in Escherichia coli. Specific enzyme activity of rMTGase was comparable to native MTGase. However, the gelation of a sodium caseinate solution induced by rMTGase was slower than that induced by native MTGase. In addition, the mechanical property of kamaboko prepared with rMTGase was weaker than that with native MTGase. In SDS-PAGE analysis, α-casein monomers decreased more slowly during the incubation with rMTGase than MTGase. These results confirmed the difference of cross-linking activity between the 2 enzymes. Furthermore, thermal stability of rMTGase was lower compared to native MTGase. These results suggest that the difference of cross-linking activity and thermal stability between the 2 enzymes cause differences in gelation activity of protein.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 0020-1693Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 0011-2240Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 0011-2240Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 0020-1693Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyType of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 0003-2697Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesMatsuo, T ; Ikura, Y ; Ohsawa, M ; Ogami, M ; Kayo, S ; Yoshimi, N ; Hai, E ; Naruko, T ; Ohishi, M ; Higuchi, K ; Arakawa, T ; Ueda, M
Oxford, UK : Blackwell Science Ltd
Published 2003Staff ViewISSN: 1365-2559Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Aims: To study the role of mast cell chymase in the inflammatory processes of human chronic gastritis. Experimental studies have shown that mast cell chymase stimulates inflammatory cell accumulation, and contributes to angiotensin II formation.Methods and results: Tissue sections from human stomachs with Helicobacter pylori-associated gastritis (surgery/autopsy n = 20; biopsy n = 16) and normal stomachs (n = 10) were studied using immunohistochemical single and double labelling techniques. Monoclonal antibodies used were directed against mast cell chymase, tryptase, neutrophils (CD66b, elastase, and myeloperoxidase), macrophages, T-lymphocytes, and interleukin (IL)-4. The expression of angiotensin-converting enzyme and angiotensin II type 1 receptor was investigated using immunohistochemical analysis and the reverse transcription-polymerase chain reaction. The number of chymase-positive mast cells was significantly higher (P 〈 0.0001) in H. pylori-associated gastritis than in normal stomachs. Increased expression of chymase in inflamed mucosa was closely related to an increase in the accumulation of neutrophils, macrophages, T-lymphocytes, and IL-4-positive cells. The expression of angiotensin-converting enzyme and angiotensin II type 1 receptor was not altered in gastritis specimens.Conclusions: These observations suggest that mast cell chymase may be an important mediator in the inflammatory processes of human H. pylori-associated gastritis.Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesOhkusa, T. ; Maekawa, T. ; Arakawa, T. ; Nakajima, M. ; Fujimoto, K. ; Hoshino, E. ; Mitachi, Y. ; Hamada, S. ; Mine, T. ; Kawahara, Y. ; Nagai, T. ; Aoyama, N. ; Yoshida, N. ; Tadokoro, K. ; Chida, N. ; Konda, Y. ; Seno, H. ; Shimatani, T. ; Inoue, M. ; Sato, N.
Oxford, UK : Blackwell Science Ltd
Published 2005Staff ViewISSN: 1365-2036Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background : The polymorphic enzyme cytochrome P450 2C19 affects omeprazole metabolism. This influence on metabolism might affect serum gastrin levels, and safety, during long-term treatment of reflux oesophagitis.Aim : To examine the relationship between cytochrome P450 2C19 genotype and the safety profile of long-term omeprazole treatment.Methods : A total of 119 Japanese patients with recurrent reflux oesophagitis underwent cytochrome P450 2C19 genotyping prior to receiving daily omeprazole 10 mg or 20 mg for 6–12 months, during which adverse event frequency, serum gastrin levels and endoscopic findings were monitored.Results : The incidences of adverse events, serious adverse events and adverse events leading to withdrawal did not differ between homozygous extensive metabolizer (n = 46), heterozygous extensive metabolizer (n = 53) or poor metabolizer (n = 20) groups. In all genotype groups, serum gastrin increased during the first 3 months of dosing but stabilized thereafter. No significant differences were seen either in the rate of reflux oesophagitis healing or symptom improvement among genotype groups.Conclusions : Long-term treatment with omeprazole was well-tolerated in Japanese patients, irrespective of their cytochrome P450 2C19 metabolic genotype, indicating that dose adjustment depending on metabolic genotype is not required during treatment with omeprazole.Type of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesTominaga, K. ; Higuchi, K. ; Sasaki, E. ; Suto, R. ; Watanabe, T. ; Fujiwara, Y. ; Oshitani, N. ; Matsumoto, T. ; Kim, S. ; Iwao, H. ; Arakawa, T.
Oxford, UK : Blackwell Publishing Ltd
Published 2004Staff ViewISSN: 1365-2036Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background : Mitogen-activated protein (MAP) kinases, including extracellular signal-regulated kinases (ERK),c-Jun NH2-terminal kinases (JNK) and p38 MAP kinase (p38 MAPK) are important intermediates of the signal-transduction pathway from the cell surface to the nucleus. Expression of cyclooxygenase (COX)-2, associated with proliferation, apoptosis or both of gastrointestinal cancer cells, is mediated through MAP kinase families. However, the correlation between respective MAP kinase signals and COX-2 in the proliferation of gastric and colon cancer cells has not been well elucidated.Aim : We examined the effect of selective inhibitors of MAP kinases and COX-2 on serum-induced proliferation of gastric (MKN45) and colon (HT29) cancer cells.Methods : After 24-h serum starvation, cancer cells were stimulated with 2% serum and COX-2 inhibitors (NS398 10 µmol/L, or etodolac 100 µmol/L) or 1 h after preincubation with inhibitors for ERK (PD98059 20 µmol/L) or p38 MAPK (SB203580 10 µmol/L). Phosphorylated MAP kinases and COX-2 protein were evaluated by Western blotting, and the proliferation of cancer cells was estimated by 3H-thymidine incorporation. Transcription factors nuclear factor-κB and CREB were assayed by an electorophoretic mobility shift assay.Results : Serum increased the proliferation of MKN45 and HT29 cells by 280% and 200%, respectively, compared with the control levels (100%). In both cancer cells, phosphorylated MAP kinases were increased within 30 min after stimulation. PD98059 and SB203580 inhibited the serum-induced proliferation of MKN45 by 21% and 51% and of HT29 by 81% and 69%, respectively. NS398 and etodolac inhibited the proliferation of HT29 by 21% and 41%, respectively, but not that of MKN45. PD98059 and SB203580 also suppressed serum-induced expression of COX-2 protein in HT29 cells. In addition to the activation of MAP kinases and COX-2, activities of nuclear factor-κB and CREB were also increased during HT29 cell proliferation.Conclusions : These results suggest that the correlation of MAP kinases with COX-2 induction for cell proliferation differs between MKN45 and HT29 cells.Type of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesFujiwara, Y. ; Higuchi, K. ; Takashima, T. ; Hamaguchi, M. ; Watanabe, T. ; Tominaga, K. ; Oshitani, N. ; Matsumoto, T. ; Arakawa, T.
Oxford UK : Blackwell Science Ltd.
Published 2002Staff ViewISSN: 1365-2036Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Epidermal growth factor (EGF), which binds to EGF receptors (EGF-R), stimulates oesophageal epithelial cell proliferation, enabling rapid repair after mucosal injury. In the normal human oesophageal epithelium, EGF-R expression is present and confined to the basal layer.〈section xml:id="abs1-2"〉〈title type="main"〉Aim:To examine histological changes in and dynamics of EGF-R expression during healing after acid reflux oesophagitis in a rat model.〈section xml:id="abs1-3"〉〈title type="main"〉Methods:Gastric acid reflux oesophagitis was induced in Wistar rats by ligation of the pylorus and the transitional region between the forestomach and the grandular portion for 5 h, followed by release of both ligations. Rats were killed 7 and 14 days after production of oesophagitis to examine macroscopic and histological changes as well as dynamics of EGF-R expression. Epithelial cell proliferation was assessed by bromodeoxyuridine (BrdU) uptake, and expression of EGF-R mRNA and protein by RT–PCR and Western blotting or immunohistochemistry.〈section xml:id="abs1-4"〉〈title type="main"〉Results:Gastric acid reflux induced erosive and ulcerative mucosal lesions in the lower and middle part of the oesophagus. These lesions were healed by 14 days and histologically showed thickening of the oesophageal epithelium from 41.11 ± 3.09 μm in controls to 142.73 ± 11.59 μm (P 〈 0.001) in ligated rats, as well as elongation of papillae and basal cell hyperplasia. The number of BrdU-positive cells among basal cells on day 14 was significantly increased from 7.1 ± 0.8/field in controls to 30.9 ± 3.0/field in ligated rats. Expression of EGF-R mRNA and protein was significantly increased on day 14 and most basal cells were immunohistochemically positive in both BrdU and EGF-R staining.〈section xml:id="abs1-5"〉〈title type="main"〉Conclusion:Acid reflux-induced oesophageal injury caused basal cell hyperplasia with an increase in cell proliferation and EGF-R expression. Activation of EGF-R gene and protein in response to acid reflux-induced injury may facilitate mucosal healing. These results suggest that epidermal growth factor receptors play a crucial role in healing after acid reflux oesophagitis in rats.Type of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesTominaga, K. ; Higuchi, K. ; Tsuno, M. ; Watanabe, T. ; Fujiwara, Y. ; Kim, S. ; Arakawa, T. ; Iwao, H. ; Kuroki, T.
Oxford, UK : Blackwell Science Ltd
Published 2000Staff ViewISSN: 1365-2036Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background: Interleukin-1β (IL-1β) participates in cell growth, differentiation and apoptosis via activation of several kinases in a variety of cells. Mitogen-activated protein (MAP) kinases are important intermediates of the signal transduction pathway from the cell surface to the nucleus, leading to activation of transcription factors. There are no reports on the effect of IL-1β on these pathways in gastric epithelial cells. Aim: To investigate whether IL-1β activates MAP kinases [extracellular signal-regulated kinases (ERKs), c-Jun NH2-terminal kinases (JNKs) and p38 MAP kinase (p38 MAPK)] and nuclear factor (NF)-κB, a transcription factor, in gastric epithelial cells (RGM1). Methods: The activities of ERKs and JNKs were estimated by in-gel kinase assay, and p38 MAPK activity was measured by in vitro kinase assay at various time points (0–40 min) after addition of IL-1β (100 pg/mL) for 20 min. The activity of NF-κB was analysed using gel mobility shift assay at times from 0 to 4 h after addition of IL-1β. Results: Activity of ERKs was detectable at 10 min, peaked at 20 min, and continued at increased levels until 40 min. Activity of both JNKs and p38 MAPK were detectable during 5–20 min, and then decreased within 40 min. Activation of NF-κB occurred at 30 min, and increased activity continued for 6 h. Interleukin-1β activated MAP kinases and NF-κB in RGM1 cells. Conclusion: The activation induced by this cytokine may play an important role in the initiation of the inflammatory process in gastric mucosa.Type of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesFujiwara, Y. ; Higuchi, K. ; Nebiki, H. ; Chono, S. ; Uno, H. ; Kitada, K. ; Satoh, H. ; Nakagawa, K. ; Kobayashi, K. ; Tominaga, K. ; Watanabe, T. ; Oshitani, N. ; Arakawa, T.
Oxford, UK : Blackwell Publishing Ltd
Published 2005Staff ViewISSN: 1365-2036Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background : Several studies in Western countries showed that proton-pump inhibitors are superior to histamine2-receptor antagonists or placebo in the treatment of non-erosive gastro-oesophageal reflux disease. The efficacy of acid-suppressive drugs for non-erosive gastro-oesophageal reflux disease in Japan, in which the prevalence of Helicobacter pylori infection is higher compared with Western countries, is unknown.Aim : To compare the efficacy of famotidine and omeprazole in Japanese patients with non-erosive gastro-oesophageal reflux disease by a prospective randomized multicentre trial.Methods : A total of 98 patients received either famotidine 20 mg b.d. (n = 48) or omeprazole once daily (n = 50). Frequency of gastro-oesophageal reflux disease symptoms and health-related quality of life were evaluated at baseline and after 4 weeks of treatment. Complete relief was defined as no gastro-oesophageal reflux disease symptoms during the 7-day interval in week 4.Results : Complete relief was achieved in 23 (48%) of patients receiving famotidine and 28 (56%) of patients treated with omeprazole. In the famotidine group, complete relief rate in H. pylori-negative patients was significantly lower than H. pylori-positive patients (35% vs. 64%). Both famotidine and omeprazole improved most scales of health-related quality of life. Omeprazole significantly improved reflux score irrespective of H. pylori infection while famotidine significantly improved reflux score in H. pylori-positive patients but not in H. pylori-negative patients.Conclusions : Omeprazole is more effective than famotidine for the control of gastro-oesophageal reflux disease symptoms in H. pylori-negative patients, while similar efficacy is observed in H. pylori-positive patients with non-erosive gastro-oesophageal reflux disease.Type of Medium: Electronic ResourceURL: