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1Latest Papers from Table of Contents or Articles in PressN. Waddell ; M. Pajic ; A. M. Patch ; D. K. Chang ; K. S. Kassahn ; P. Bailey ; A. L. Johns ; D. Miller ; K. Nones ; K. Quek ; M. C. Quinn ; A. J. Robertson ; M. Z. Fadlullah ; T. J. Bruxner ; A. N. Christ ; I. Harliwong ; S. Idrisoglu ; S. Manning ; C. Nourse ; E. Nourbakhsh ; S. Wani ; P. J. Wilson ; E. Markham ; N. Cloonan ; M. J. Anderson ; J. L. Fink ; O. Holmes ; S. H. Kazakoff ; C. Leonard ; F. Newell ; B. Poudel ; S. Song ; D. Taylor ; S. Wood ; Q. Xu ; J. Wu ; M. Pinese ; M. J. Cowley ; H. C. Lee ; M. D. Jones ; A. M. Nagrial ; J. Humphris ; L. A. Chantrill ; V. Chin ; A. M. Steinmann ; A. Mawson ; E. S. Humphrey ; E. K. Colvin ; A. Chou ; C. J. Scarlett ; A. V. Pinho ; M. Giry-Laterriere ; I. Rooman ; J. S. Samra ; J. G. Kench ; J. A. Pettitt ; N. D. Merrett ; C. Toon ; K. Epari ; N. Q. Nguyen ; A. Barbour ; N. Zeps ; N. B. Jamieson ; J. S. Graham ; S. P. Niclou ; R. Bjerkvig ; R. Grutzmann ; D. Aust ; R. H. Hruban ; A. Maitra ; C. A. Iacobuzio-Donahue ; C. L. Wolfgang ; R. A. Morgan ; R. T. Lawlor ; V. Corbo ; C. Bassi ; M. Falconi ; G. Zamboni ; G. Tortora ; M. A. Tempero ; A. J. Gill ; J. R. Eshleman ; C. Pilarsky ; A. Scarpa ; E. A. Musgrove ; J. V. Pearson ; A. V. Biankin ; S. M. Grimmond
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-02-27Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Adenocarcinoma/drug therapy/genetics ; Animals ; Carcinoma, Pancreatic Ductal/drug therapy/genetics ; *DNA Mutational Analysis ; DNA Repair/genetics ; Female ; Genes, BRCA1 ; Genes, BRCA2 ; Genetic Markers/genetics ; Genome, Human/*genetics ; Genomic Instability/genetics ; *Genomics ; Genotype ; Humans ; Mice ; Mutation/*genetics ; Pancreatic Neoplasms/classification/drug therapy/*genetics ; Platinum/pharmacology ; Point Mutation/genetics ; Poly(ADP-ribose) Polymerase Inhibitors ; Xenograft Model Antitumor AssaysPublished by: -
2Latest Papers from Table of Contents or Articles in PressP. Bailey ; D. K. Chang ; K. Nones ; A. L. Johns ; A. M. Patch ; M. C. Gingras ; D. K. Miller ; A. N. Christ ; T. J. Bruxner ; M. C. Quinn ; C. Nourse ; L. C. Murtaugh ; I. Harliwong ; S. Idrisoglu ; S. Manning ; E. Nourbakhsh ; S. Wani ; L. Fink ; O. Holmes ; V. Chin ; M. J. Anderson ; S. Kazakoff ; C. Leonard ; F. Newell ; N. Waddell ; S. Wood ; Q. Xu ; P. J. Wilson ; N. Cloonan ; K. S. Kassahn ; D. Taylor ; K. Quek ; A. Robertson ; L. Pantano ; L. Mincarelli ; L. N. Sanchez ; L. Evers ; J. Wu ; M. Pinese ; M. J. Cowley ; M. D. Jones ; E. K. Colvin ; A. M. Nagrial ; E. S. Humphrey ; L. A. Chantrill ; A. Mawson ; J. Humphris ; A. Chou ; M. Pajic ; C. J. Scarlett ; A. V. Pinho ; M. Giry-Laterriere ; I. Rooman ; J. S. Samra ; J. G. Kench ; J. A. Lovell ; N. D. Merrett ; C. W. Toon ; K. Epari ; N. Q. Nguyen ; A. Barbour ; N. Zeps ; K. Moran-Jones ; N. B. Jamieson ; J. S. Graham ; F. Duthie ; K. Oien ; J. Hair ; R. Grutzmann ; A. Maitra ; C. A. Iacobuzio-Donahue ; C. L. Wolfgang ; R. A. Morgan ; R. T. Lawlor ; V. Corbo ; C. Bassi ; B. Rusev ; P. Capelli ; R. Salvia ; G. Tortora ; D. Mukhopadhyay ; G. M. Petersen ; D. M. Munzy ; W. E. Fisher ; S. A. Karim ; J. R. Eshleman ; R. H. Hruban ; C. Pilarsky ; J. P. Morton ; O. J. Sansom ; A. Scarpa ; E. A. Musgrove ; U. M. Bailey ; O. Hofmann ; R. L. Sutherland ; D. A. Wheeler ; A. J. Gill ; R. A. Gibbs ; J. V. Pearson ; A. V. Biankin ; S. M. Grimmond
Nature Publishing Group (NPG)
Published 2016Staff ViewPublication Date: 2016-02-26Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Basic Helix-Loop-Helix Transcription Factors/genetics ; Carcinoma, Pancreatic ; Ductal/classification/genetics/immunology/metabolism/pathology ; Cell Line, Tumor ; DNA Methylation ; DNA-Binding Proteins/genetics ; Gene Expression Regulation, Neoplastic ; Gene Regulatory Networks ; Genes, Neoplasm/*genetics ; Genome, Human/*genetics ; *Genomics ; Hepatocyte Nuclear Factor 3-beta/genetics ; Hepatocyte Nuclear Factor 3-gamma/genetics ; Histone Demethylases/genetics ; Homeodomain Proteins/genetics ; Humans ; Mice ; Mutation/*genetics ; Nuclear Proteins/genetics ; Pancreatic Neoplasms/*classification/*genetics/immunology/metabolism/pathology ; Prognosis ; Receptors, Cytoplasmic and Nuclear/genetics ; Survival Analysis ; Trans-Activators/genetics ; Transcription Factors/genetics ; Transcription, Genetic ; Transcriptome ; Tumor Suppressor Protein p53/genetics ; Tumor Suppressor Proteins/geneticsPublished by: -
3Latest Papers from Table of Contents or Articles in PressA. V. Biankin ; N. Waddell ; K. S. Kassahn ; M. C. Gingras ; L. B. Muthuswamy ; A. L. Johns ; D. K. Miller ; P. J. Wilson ; A. M. Patch ; J. Wu ; D. K. Chang ; M. J. Cowley ; B. B. Gardiner ; S. Song ; I. Harliwong ; S. Idrisoglu ; C. Nourse ; E. Nourbakhsh ; S. Manning ; S. Wani ; M. Gongora ; M. Pajic ; C. J. Scarlett ; A. J. Gill ; A. V. Pinho ; I. Rooman ; M. Anderson ; O. Holmes ; C. Leonard ; D. Taylor ; S. Wood ; Q. Xu ; K. Nones ; J. L. Fink ; A. Christ ; T. Bruxner ; N. Cloonan ; G. Kolle ; F. Newell ; M. Pinese ; R. S. Mead ; J. L. Humphris ; W. Kaplan ; M. D. Jones ; E. K. Colvin ; A. M. Nagrial ; E. S. Humphrey ; A. Chou ; V. T. Chin ; L. A. Chantrill ; A. Mawson ; J. S. Samra ; J. G. Kench ; J. A. Lovell ; R. J. Daly ; N. D. Merrett ; C. Toon ; K. Epari ; N. Q. Nguyen ; A. Barbour ; N. Zeps ; N. Kakkar ; F. Zhao ; Y. Q. Wu ; M. Wang ; D. M. Muzny ; W. E. Fisher ; F. C. Brunicardi ; S. E. Hodges ; J. G. Reid ; J. Drummond ; K. Chang ; Y. Han ; L. R. Lewis ; H. Dinh ; C. J. Buhay ; T. Beck ; L. Timms ; M. Sam ; K. Begley ; A. Brown ; D. Pai ; A. Panchal ; N. Buchner ; R. De Borja ; R. E. Denroche ; C. K. Yung ; S. Serra ; N. Onetto ; D. Mukhopadhyay ; M. S. Tsao ; P. A. Shaw ; G. M. Petersen ; S. Gallinger ; R. H. Hruban ; A. Maitra ; C. A. Iacobuzio-Donahue ; R. D. Schulick ; C. L. Wolfgang ; R. A. Morgan ; R. T. Lawlor ; P. Capelli ; V. Corbo ; M. Scardoni ; G. Tortora ; M. A. Tempero ; K. M. Mann ; N. A. Jenkins ; P. A. Perez-Mancera ; D. J. Adams ; D. A. Largaespada ; L. F. Wessels ; A. G. Rust ; L. D. Stein ; D. A. Tuveson ; N. G. Copeland ; E. A. Musgrove ; A. Scarpa ; J. R. Eshleman ; T. J. Hudson ; R. L. Sutherland ; D. A. Wheeler ; J. V. Pearson ; J. D. McPherson ; R. A. Gibbs ; S. M. Grimmond
Nature Publishing Group (NPG)
Published 2012Staff ViewPublication Date: 2012-10-30Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Axons/*metabolism ; Carcinoma, Pancreatic Ductal/*genetics/*pathology ; Gene Dosage ; Gene Expression Regulation, Neoplastic ; Genome/*genetics ; Humans ; Kaplan-Meier Estimate ; Mice ; Mutation ; Pancreatic Neoplasms/*genetics/*pathology ; Proteins/genetics ; Signal TransductionPublished by: -
4Latest Papers from Table of Contents or Articles in PressLin, F., Wani, S. H., Collins, P. J., Wen, Z., Gu, C., Chilvers, M. I., Wang, D.
Genetics Society of America (GSA)
Published 2018Staff ViewPublication Date: 2018-10-04Publisher: Genetics Society of America (GSA)Electronic ISSN: 2160-1836Topics: BiologyPublished by: -
5Articles: DFG German National LicensesWANI, S. ; SAMPLINER, R. E. ; WESTON, A. P. ; MATHUR, S. ; HALL, M. ; HIGBEE, A. ; SHARMA, P.
Oxford, UK : Blackwell Science Ltd
Published 2005Staff ViewISSN: 1365-2036Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background : Barrett's oesophagus patients may continue to have abnormal oesophageal acid exposure on proton pump inhibitor therapy. The effect of factors such as Barrett's oesophagus length, hiatal hernia size and Helicobacter pylori infection on intra-oesophageal pH in Barrett's oesophagus patients has not been adequately studied.Aim : To evaluate oesophageal acid exposure in a large Barrett's oesophagus population on b.d. proton pump inhibitor therapy and determine clinical factors predicting normalization of intra-oesophageal pH on therapy.Methods : Barrett's oesophagus patients were studied using 24 h pH monitoring to evaluate intra-oesophageal acid suppression on b.d. dosing of rabeprazole.Results : Forty-six Barrett's oesophagus patients completed the study. Median total percentage time pH 〈 4 was 1.05% (range: 0–29.9%) in the entire group and respective values for upright and supine percentage time pH 〈 4 were 1.15% and 0%. However, 34 of the Barrett's oesophagus patients (73.9%) had a normal pH study (median total percentage time pH 〈 4: 0.2%) and 12 patients (26.1%) had an abnormal result (median total percentage time pH 〈 4: 9.3%). There were no significant differences between patients with a normal and abnormal 24 h pH result with respect to age, Barrett's oesophagus length, hiatal hernia size and presence of H. pylori infection.Conclusions : Approximately 25% of Barrett's oesophagus patients continue to have abnormal total intra-oesophageal pH profiles despite b.d. proton pump inhibitor therapy. Factors such as age, Barrett's oesophagus length and hiatal hernia size cannot be used to predict persistent abnormal intra-oesophageal pH on proton pump inhibitor.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1432-0789Keywords: 15N ; N2 fixation ; Rhizosphere ; Sorghum bicolor ; Pennisetum americanum ; Acetylene reduction assay (ARA)Source: Springer Online Journal Archives 1860-2000Topics: BiologyGeosciencesAgriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary In a series of short-term experiments root systems of young sorghum and millet plants inoculated with N2-fixing bacteria were exposed to 15N2-enriched atmospheres for 72 h. The plants were grown in a normal atmosphere for up to 22 days after the end of the exposure to allow them to take up the fixed N2. Environmental conditions and genotypes of sorghum and millet were selected to maximise N2-fixation in the rhizosphere. Detectable amounts of fixed N (〉 16 μg/plant) were rapidly incorporated into sorghum plants grown in a sand/farmyard manure medium, but measurable fixation was found on only one occasion in plants grown in soil. N2 fixation was detectable in some experiments with soil-grown millet plants but the amounts were small (2–4 μg/plant) and represented less than 1 % of plant N accumulated over the same period. In many cases there was no detectable 15N2 incorporation despite measurable increases in ethylene concentration found during an acetylene reduction assay.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 1432-0789Keywords: Agroecological rotation ; Hordeum vulgare ; Microbial biomass ; 15N ; Rotation effects ; Pot experiment ; Soil nitrogenSource: Springer Online Journal Archives 1860-2000Topics: BiologyGeosciencesAgriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary Soil N dynamics and barley yields (Hordeum vulgare L.) were compared in pot experiments using surface samples from a Gray Luvisol under three cropping systems at Breton, Alberta: (1) an agroecological 8-year rotation including cereals, forage, and fababeans (Vicia faba L.) as green manure, from wich two plots were selected, one following fababeans, and the second following 3 years of forage; (2) a continuous grain system, with fertilizer N at 90 kg ha-1 year-1; and (3) a classical Breton 5-year rotation [following oats (Avena sativa L.)] involving forage and cereals, without returning crop residues to the land, selecting one plot with PKS treatment and a second as control. The fertilizer N equivalent for the cropping system; “AN” value and “A” value (analogous to AN), but in fertilizer 15N units, soil biomass, and C and N mineralization were monitored. In the first agroecological plot (after fababeans), grain and total plant biomass production were 116% greater than from the continuous grain treatment. Barley plants in the two agroecological plots derived 48.5% and 37.8%, respectively, of their N requirement from non-labelled soil N sources not present in the continuous grain plot. At crop maturity, the recovery of 15N microbial biomass was 1.5 times higher in soil from the first agroecological plot than from the continuous grain plot. The fertilizer N equivalent was 2670 mg pot-1 (485 kg ha-1) for the first and 1850 mg pot-1 for the second agroecological treatment. Fertilizer N equivalent values exceed net amounts of N mineralized by a factor of 4. Recovery by the barley crop of 15N added at 55 mg pot-1 was more efficient in the agroecological treatments (45%–51%) than in the continuous grain or classical Breton treatments (35%–37%). It was concluded (1) that past soil history may be associated more with the ability of barley plants to compete for available N, and hence the use of N, than with net soil N mineralization; and (2) an increased supply of N to crops following the incorporation of fababean residues, manure application, and the soil N-conserving effect of growing legumes were all partly responsible for the observed differences in soil fertility.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1432-0789Keywords: Bipolaris sorokiniana ; Common root rot ; Crop rotation ; Hordeum vulgare ; VAM ; Breton plotsSource: Springer Online Journal Archives 1860-2000Topics: BiologyGeosciencesAgriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary This paper presents soil biological data from a study on the functioning of three soil-plant systems on a Gray Luvisol in Cryoboreal Subhumid central Alberta. The systems were (1) an agroecological 8-year rotation, (2) a continuous grain system, both established in 1981, and (3) a classical Breton 5-year rotation established in 1930. The objectives were to (1) determine whether changes in vesicular-arbuscular mycorrhizae (VAM) populations occurred in soil under these cropping systems, (2) discover whether these cropping systems and/or VAM infection influenced the incidence of common root rot (Bipolaris sorokiniana), and (3) use nutrient translocation indices to test the hypothesis that soil quality influences non-specific physiological conditions in barley (Hordeum vulgare L.). VAM fungal propagules in soil samples and VAM infection under controlled conditions were significantly affected by the cropping system. VAM infection accounted for more than 85% of the variability in grain yield, plant biomass yield, and plant uptake of K, S, Ca, Fe, and Zn under controlled conditions. Backward-elimination regression analyses showed that under these conditions of high available P, plant P uptake was governed by the quantity of extractable P in the soil (r 2=0.82); the VAM infection contributed practically nothing when combined with available P (R 2=0.84). Neither VAM infection nor the cropping system were related to the B. sorokiniana infection in the barley. The growth of B. sorokiniana was equal, and its sporulation superior, when grown on residues of the non-host fababean (Vicia faba L.), compared with growth on residues of barley. Higher translocation of plant nutrients to the grain in the agroecological compared with the continuous grain treatments suggested that VAM and/or the soil history affected plant physiology, possible through hormonal effects. Superior barley yields in the agroecological compared with the continuous grain treatments were partly due to increased VAM colonization, greater nutrient accumulation and translocation to the grain, but not to a reduced disease incidence. These results demonstrate the benefits of a holistic systems approach while studying biological interactions involving plants and groups of soil microorganisms.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Keywords: cropping system ; legumes ; N mineralization ; N potential ; rotation effectsSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Abstract The quantity and patterns of net mineralization of soil nitrogen (N) were studied in Vertisols under different cropping systems in the semi-arid tropical areas. Eight cropping systems were selected; three contained pigeonpea (PP), one contained PP and cowpea (COP), and two contained chickpea (CP) as legume component crops, one included sequence cropping with nonlegumes during the rainy and postrainy seasons, and one system was kept fallow (F) during the rainy season and sown to sorghum (S) during the postrainy season. Cropping systems with PP as a component crop increased mineralizable N(N o ) content two-fold in the soil compared with fallow + sorghum (F+S)−F+S system. The N mineralization rate constant (k) was not significantly affected by previous cropping history of the soil; however, a numerically higher rate constant was observed in the COP/PP intercrop, followed by sequential S+safflower (SF) system as compared to the other soils. Mineral N accumulation curves for six soils were more accurately described by the exponential model than the linear model. The active N fraction (N o /Ntot %) varied between 8 and 16% for different systems and a direct relationship was observed between N o /Ntot and total N for the soils under diverse cropping systems.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Source: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary The screening of micro-organisms decomposing wheat straw was done under laboratory conditions on the basis of the amount of carbon mineralized from wheat straw during a period of four months. In general, inoculation of wheat straw with different micro-organisms had favorable effect on the amount of carbon mineralized. Among the bacterial and fungus cultures isolated from baited wheat straw an Aspergillus sp. (Isolate No. 18) proved to be the most rapid wheat straw decomposer under laboratory conditions. re]19751008Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Keywords: ELISA ; grain yield ; inoculation ; millet ; N2-fixing bacteria ; NO3 reductaseSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Abstract Responses to inoculation with N2-fixing bacteria were studied in relation to genotypic differences in pearl millet, effect of nitrogen levels, and FYM additions in India. In some experiments, inoculation increased mean grain yield up to 33% over the uninoculated control, whereas in the remaining 11 experiments there was no significant increase. Increased grain yields, 〉10% over the uninoculated controls were observed in 46% of the experiments withAzospirillum lipoferum (18.7% average increase) and withAzotobacter chroococcum (13.6% average increase). Yield increases were nil or reduced in three experiments withAzos. lipoferum and four experiments withAztb. chroococcum. In two experiments continued inoculation for two or three years resulted in increased grain, plant biomass yield, and N uptake. Interactions of bacterial cultures with cultivars or years were not observed. The counts of the inoculated strains increased two to three-fold when inoculation was continued for three years. Repeated inoculations increased the mean cumulative N uptake from season 1 to season 3 by 19 kg ha−1. Repeated inoculations withAztb. chroococcum andAzos. lipoferum increased mean grain yield of a succeeding crop by 14.4% and 9.8%, respectively, over the uninoculated control. Inoculation increased the efficiency of N-assimilation by pearl millet. Marginal increase in nitrogenase activity, associated with the inoculated plants was observed during later stages of plant growth. Increased leaf nitrate reductase activity (NRA) was observed after inoculation with these bacteria. The responses to inoculation are mainly attributable to increased plant N assimilation which could be the effect of growth promoting substances secreated by the bacteria; and thus the contribution from BNF may be small.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Keywords: inoculation ; legumes ; nonnodulation ; rhizobia ; residual effects ; symbiotic N2 fixationSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Abstract Sustainable agriculture relies greatly on renewable resources like biologically fixed nitrogen. Biological nitrogen fixation plays an important role in maintaining soil fertility. However, as BNF is dependent upon physical, environmental, nutritional and biological factors, mere inclusion of any N2-fixing plant system does not guarantee increased contributions to the soil N pool. In the SAT where plant stover is also removed to feed animals, most legumes might be expected to deplete soil N. Yet beneficial legume effects in terms of increased yields in succeeding cereal crops have been reported. Such benefits are partly due to N contribution from legumes through BNF and soil N saving effect. In addition, other non-N rotational benefits, for example, improved nutrient availability, improved soil structure, reduced pests and diseases, hormonal effects are also responsible. In this paper we have reviewed the research on the contribution of grain legumes in cropping systems and the factors affecting BNF. Based on the information available, we have suggested ways for exploiting BNF for developing sustainable agriculture in the semi-arid tropics (SAT). A holistic approach involving host-plant, bacteria, environment and proper management practices including need based inoculation for enhancing BNF in the cropping systems in the SAT is suggested.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Keywords: C2H2 reduction ; Intact-plant assay ; Millet ; Nitrogen fixation ; Nitrogenase activity ; SorghumSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary A non destructive intact-plant assay for estimating nitrogenase activity (C2H2 reduction) of pot-grown sorghum and millet plants is described. Plants with intact shoots sustained more activity than plants whose tops were removed prior to the assay. With this technique individual plants can be assayed several times during their life cycle. The C2H2 reduction was linear up to 16h incubation in this assay procedure. More rapid diffusion of C2H2 was achieved by injection through a Suba seal in the bottom of the pot. The equlibration of injected C2H2 in the gas phase of the pots filled with sand and sand:FYM media was completed within 1 h. Significantly higher nitrogenase activity and better growth of sorghum and millet plants occurred when plants were grown in a mixture of sand and farmyard manure (FYM) than when plants were grown in vermiculite, soil, or sand + soil medium. Nitrogenase activity and plant growth were greater in a mixture of sand with 2 and 3% FYM than with 0.5 and 1% FYM. Activity was higher when the plants were incubated at 33°C and 40°C than at 27°C. Activity also increased with increasing soil moisture. There were significant differences amongst 15 sorghum cultivars screened for associated nitrogenase activity. This new technique has good prospects for screening cultivars of millet, sorghum and other grain crops for their nitrogen-fixing ability.Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 1573-5036Keywords: Vermiculite ; Millet ; 15N Nitrogen fixation (associative) ; Non-exchangeable-NSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary Genotypes of sorghum and millet have previously been found to have different amounts of root-associated acetylene reduction activity. Isotope dilution experiments using15N have been carried out to evaluate the amount of nitrogen fixed by bacteria which is incorporated into the different genotypes when grown in vermiculite in the glasshouse. Isotope dilution result indicated that the content of shoot nitrogen derived from biological nitrogen fixation varied by up to 27% between sorghum genotypes and 17% between millet genotypes. Considerable isotope dilution also resulted from uptake of non-exchangeable-N (as NH 4 + ) in the vermiculite. It is possible that the genotypic differences in isotope dilution may reflect differences in the ability of plants to take up non-exchangeable-N, and that vermicultite is therefore an unsuitable growth medium for such studies.Type of Medium: Electronic ResourceURL: