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1Latest Papers from Table of Contents or Articles in PressStaff View
Publication Date: 2018-01-05Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyGeosciencesComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Immunology, Medicine, DiseasesPublished by: -
2Latest Papers from Table of Contents or Articles in PressL. O. Gostin ; A. Phelan ; M. A. Stoto ; J. D. Kraemer ; K. S. Reddy
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-09-13Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; *Disaster Planning ; *Global Health ; Health Services Accessibility ; Humans ; Influenza A Virus, H1N1 Subtype/*genetics ; Influenza A Virus, H5N1 Subtype/*genetics ; *Influenza Vaccines ; Influenza in Birds/epidemiology/prevention & control ; Influenza, Human/epidemiology/*prevention & control/virology ; Intellectual Property ; Orthomyxoviridae Infections/prevention & control/virology ; Pandemics/*prevention & control/veterinary ; Poultry ; Sequence Analysis, DNA ; SwinePublished by: -
3Latest Papers from Table of Contents or Articles in PressB. G. Modi ; J. Neustadter ; E. Binda ; J. Lewis ; R. B. Filler ; S. J. Roberts ; B. Y. Kwong ; S. Reddy ; J. D. Overton ; A. Galan ; R. Tigelaar ; L. Cai ; P. Fu ; M. Shlomchik ; D. H. Kaplan ; A. Hayday ; M. Girardi
American Association for the Advancement of Science (AAAS)
Published 2012Staff ViewPublication Date: 2012-01-10Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: 9,10-Dimethyl-1,2-benzanthracene/*analogs & derivatives/metabolism/toxicity ; Animals ; Aryl Hydrocarbon Hydroxylases/metabolism ; Carcinogens/*metabolism/*toxicity ; Carcinoma, Squamous Cell/*chemically induced/metabolism ; Cell Transformation, Neoplastic ; Cells, Cultured ; Cytochrome P-450 CYP1A1/metabolism ; Cytochrome P-450 CYP1B1 ; *DNA Damage ; Genes, ras ; Humans ; Keratinocytes/metabolism/pathology ; Langerhans Cells/immunology/*metabolism ; Mice ; Mice, Transgenic ; Skin Neoplasms/*chemically induced/metabolism ; T-Lymphocytes/immunologyPublished by: -
4Latest Papers from Table of Contents or Articles in PressC. Rotimi ; A. Abayomi ; A. Abimiku ; V. M. Adabayeri ; C. Adebamowo ; E. Adebiyi ; A. D. Ademola ; A. Adeyemo ; D. Adu ; D. Affolabi ; G. Agongo ; S. Ajayi ; S. Akarolo-Anthony ; R. Akinyemi ; A. Akpalu ; M. Alberts ; O. Alonso Betancourt ; A. M. Alzohairy ; G. Ameni ; O. Amodu ; G. Anabwani ; K. Andersen ; F. Arogundade ; O. Arulogun ; D. Asogun ; R. Bakare ; N. Balde ; M. L. Baniecki ; C. Beiswanger ; A. Benkahla ; L. Bethke ; M. Boehnke ; V. Boima ; J. Brandful ; A. I. Brooks ; F. C. Brosius ; C. Brown ; B. Bucheton ; D. T. Burke ; B. G. Burnett ; S. Carrington-Lawrence ; N. Carstens ; J. Chisi ; A. Christoffels ; R. Cooper ; H. Cordell ; N. Crowther ; T. Croxton ; J. de Vries ; L. Derr ; P. Donkor ; S. Doumbia ; A. Duncanson ; I. Ekem ; A. El Sayed ; M. E. Engel ; J. C. Enyaru ; D. Everett ; F. M. Fadlelmola ; E. Fakunle ; K. H. Fischbeck ; A. Fischer ; O. Folarin ; J. Gamieldien ; R. F. Garry ; S. Gaseitsiwe ; R. Gbadegesin ; A. Ghansah ; M. Giovanni ; P. Goesbeck ; F. X. Gomez-Olive ; D. S. Grant ; R. Grewal ; M. Guyer ; N. A. Hanchard ; C. T. Happi ; S. Hazelhurst ; B. J. Hennig ; C. Hertz ; Fowler ; W. Hide ; F. Hilderbrandt ; C. Hugo-Hamman ; M. E. Ibrahim ; R. James ; Y. Jaufeerally-Fakim ; C. Jenkins ; U. Jentsch ; P. P. Jiang ; M. Joloba ; V. Jongeneel ; F. Joubert ; M. Kader ; K. Kahn ; P. Kaleebu ; S. H. Kapiga ; S. K. Kassim ; I. Kasvosve ; J. Kayondo ; B. Keavney ; A. Kekitiinwa ; S. H. Khan ; P. Kimmel ; M. C. King ; R. Kleta ; M. Koffi ; J. Kopp ; M. Kretzler ; J. Kumuthini ; S. Kyobe ; C. Kyobutungi ; D. T. Lackland ; K. A. Lacourciere ; G. Landoure ; R. Lawlor ; T. Lehner ; M. Lesosky ; N. Levitt ; K. Littler ; Z. Lombard ; J. F. Loring ; S. Lyantagaye ; A. Macleod ; E. B. Madden ; C. R. Mahomva ; J. Makani ; M. Mamven ; M. Marape ; G. Mardon ; P. Marshall ; D. P. Martin ; D. Masiga ; R. Mason ; M. Mate-Kole ; E. Matovu ; M. Mayige ; B. M. Mayosi ; J. C. Mbanya ; S. A. McCurdy ; M. I. McCarthy ; H. McIlleron ; S. O. Mc'Ligeyo ; C. Merle ; A. O. Mocumbi ; C. Mondo ; J. V. Moran ; A. Motala ; M. Moxey-Mims ; W. S. Mpoloka ; C. L. Msefula ; T. Mthiyane ; N. Mulder ; G. Mulugeta ; D. Mumba ; J. Musuku ; M. Nagdee ; O. Nash ; D. Ndiaye ; A. Q. Nguyen ; M. Nicol ; O. Nkomazana ; S. Norris ; B. Nsangi ; A. Nyarko ; M. Nyirenda ; E. Obe ; R. Obiakor ; A. Oduro ; S. F. Ofori-Acquah ; O. Ogah ; S. Ogendo ; K. Ohene-Frempong ; A. Ojo ; T. Olanrewaju ; J. Oli ; C. Osafo ; O. Ouwe Missi Oukem-Boyer ; B. Ovbiagele ; A. Owen ; M. O. Owolabi ; L. Owolabi ; E. Owusu-Dabo ; G. Pare ; R. Parekh ; H. G. Patterton ; M. B. Penno ; J. Peterson ; R. Pieper ; J. Plange-Rhule ; M. Pollak ; J. Puzak ; R. S. Ramesar ; M. Ramsay ; R. Rasooly ; S. Reddy ; P. C. Sabeti ; K. Sagoe ; T. Salako ; O. Samassekou ; M. S. Sandhu ; O. Sankoh ; F. S. Sarfo ; M. Sarr ; G. Shaboodien ; I. Sidibe ; G. Simo ; M. Simuunza ; L. Smeeth ; E. Sobngwi ; H. Soodyall ; H. Sorgho ; O. Sow Bah ; S. Srinivasan ; D. J. Stein ; E. S. Susser ; C. Swanepoel ; G. Tangwa ; A. Tareila ; O. Tastan Bishop ; B. Tayo ; N. Tiffin ; H. Tinto ; E. Tobin ; S. M. Tollman ; M. Traore ; M. J. Treadwell ; J. Troyer ; M. Tsimako-Johnstone ; V. Tukei ; I. Ulasi ; N. Ulenga ; B. van Rooyen ; A. P. Wachinou ; S. P. Waddy ; A. Wade ; M. Wayengera ; J. Whitworth ; L. Wideroff ; C. A. Winkler ; S. Winnicki ; A. Wonkam ; M. Yewondwos ; T. sen ; N. Yozwiak ; H. Zar
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-06-21Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Africa ; Disease/*genetics ; England ; Genetics, Medical/trends ; Genome-Wide Association Study/*trends ; Genomics/*trends ; Health ; Humans ; National Institutes of Health (U.S.) ; United StatesPublished by: -
5Latest Papers from Table of Contents or Articles in PressStaff View
Publication Date: 2018-10-23Publisher: American Physical Society (APS)Print ISSN: 0556-2813Electronic ISSN: 1089-490XTopics: PhysicsKeywords: Nuclear AstrophysicsPublished by: -
6FIS Bildung LiteraturdatenbankStaff View
Type of Medium: articlePublication Date: 2007Keywords: Unternehmen ; Arbeitsmarktpolitik ; Unternehmer ; Weiterbildung ; IndienIn: Developmental aspects of entrepreneurship., New Delhi u.a.: Response Books (2007), S. 178-194Language: English -
7Latest Papers from Table of Contents or Articles in PressP. Chenga Reddy, S. Arumugam, P. Nithin Sai Krishna and N. Jaya Sainath
Institute of Physics (IOP)
Published 2018Staff ViewPublication Date: 2018-07-31Publisher: Institute of Physics (IOP)Print ISSN: 1757-8981Electronic ISSN: 1757-899XTopics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsPublished by: -
8Latest Papers from Table of Contents or Articles in PressSrinivas Angadi, K Sudhakar Reddy, S Selvaprakash, J S R Prasad and M Venu
Institute of Physics (IOP)
Published 2018Staff ViewPublication Date: 2018-11-09Publisher: Institute of Physics (IOP)Print ISSN: 1757-8981Electronic ISSN: 1757-899XTopics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsPublished by: -
9Latest Papers from Table of Contents or Articles in PressP. Chenga Reddy, S. Arumugam, M. Lalith Babu and V.V.K. Krishna Teja
Institute of Physics (IOP)
Published 2018Staff ViewPublication Date: 2018-07-31Publisher: Institute of Physics (IOP)Print ISSN: 1757-8981Electronic ISSN: 1757-899XTopics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsPublished by: -
10Articles: DFG German National LicensesReddy, S. Paddi ; Prasad, C. V. V.
College Park, Md. : American Institute of Physics (AIP)
Published 1989Staff ViewISSN: 1089-7690Source: AIP Digital ArchiveTopics: PhysicsChemistry and PharmacologyNotes: The rotational structure of the 1–0, 0–0, and 0–1 bands of the Baldet–Johnson (B 2Σ+–A 2Πi) system of the molecular ion 13C18O+, excited in the cathode glow of a hollow-cathode discharge tube, was recorded in the spectral region 3700–4225 A(ring) and analyzed. An effective Hamiltonian proposed by Brown et al. [J. M. Brown, E. A. Colbourn, J. K. G. Watson, and F. D. Wayne, J. Mol. Spectrosc. 74, 294 (1979)] was used to obtain the molecular constants from the wave number data of these three bands. These molecular constants were first "merged'' together and the resulting constants were then "merged'' with those of the comet-tail (A 2Πi–X 2Σ+) system of 13C18O+ recently reported by us [C. V. V. Prasad and S. P. Reddy, J. Chem. Phys. 90, 3010 (1989)] to obtain a unique set of constants for the X 2Σ+, A 2Πi, and B 2Σ+ states. Some of the derived constants of 13C18O+ (in cm−1) are for X 2Σ+: Be=1.794 28(4), αe=0.016 61(3), ωe =2110.27(2), and ωexe =13.81(6); for A 2Πi : Be=1.441 92(4), αe=0.016 76(1), ωe =1488.99(2), ωexe =12.454(7), and ωeye =0.0279(8); for B 2Σ+: Be=1.632 62(4), αe=0.025 49(2), ωe=1657.56, and ωexe =27.42.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesPrasad, C. V. V. ; Reddy, S. Paddi
College Park, Md. : American Institute of Physics (AIP)
Published 1989Staff ViewISSN: 1089-7690Source: AIP Digital ArchiveTopics: PhysicsChemistry and PharmacologyNotes: The comet-tail (A2Πi–X2Σ+) system of the molecular ion 13C18O+, excited in the cathode glow of a hollow-cathode discharge tube, was recorded in the spectral region 3620–6165 A(ring). Of the nine bands observed with v'=0 to 5 and v‘=0 to 2, the rotational structure of 8 bands was analyzed. Brown's effective Hamiltonian [J. M. Brown, E. A. Colbourn, J. K. G. Watson, and F. D. Wayne, J. Mol. Spectrosc. 74, 294 (1979)] was used to obtain the molecular constants of the individual bands from their wave number data. By "merging'' the molecular constants thus obtained, a unique set of constants for the X2Σ+ and A2Πi states was obtained. Some of the derived molecular constants of 13C18O+ in units of cm−1 (except re in A(ring)) are: X2Σ+: Be=1.794 28(5), re=1.1156, αe=0.016 66(3), ΔG(1/2)=2082.69; A2Πi: Be=1.441 73(5), re=1.2445, αe=0.016 71(1), ωe=1488.07, ωexe=12.16.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 1525-1314Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: GeosciencesNotes: Shape, size and orientation measurements of quartz grains sampled along two transects that cross zones of increasing metamorphic grade in the Otago Schist, New Zealand, reveal the role of quartz in the progressive development of metamorphic foliation. Sedimentary compaction and diagenesis contributed little to the formation of a shape-preferred orientation (SPO) within the analysed samples. Metamorphic foliation was initiated at sub-greenschist facies conditions as part of a composite S1-bedding structure parallel to the axial planes of tight to isoclinal F1 folds. An important component of this foliation is a pronounced quartz SPO that formed dominantly by the effect of dissolution–precipitation creep on detrital grains in association with F1 strain. With increasing grade, the following trends are evident from the SPO data: (i) a progressive increase in the aspect ratio of grains in sections parallel to lineation, and the development of blade-shaped grains; (ii) the early development of a strong shape preferred orientation so that blade lengths define the linear aspect of the foliation (lineation) and the intermediate axes of the blades define a partial girdle about the lineation; (iii) a slight thinning and reduction in volume of grains in the one transect; and (iv) an actual increase in thickness and volume in the survivor grains of the second transect. The highest-grade samples, within the chlorite zone of the greenschist facies, record segregation into quartz- and mica-rich layers. This segregation resulted largely from F2 crenulation and marks a key change in the distribution, deformation and SPO of the quartz grains. The contribution of quartz SPO to defining the foliation lessens as the previously discrete and aligned detrital quartz grains are replaced by aggregates and layers of dynamically recrystallized quartz grains of reduced aspect ratio and reduced alignment. Pressure solution now affects the margins of quartz-rich layers rather than individual grains. In higher-grade samples, therefore, the rock structure is characterized increasingly by segregation layering parallel to a foliation defined predominantly by mica SPO.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1525-1314Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: GeosciencesNotes: Two samples from the Eclogite Micaschist Complex (EMC) and the Seconda Zona Diorito–Kinzigitica (IIDK) of the Sesia Zone have been studied using a high-spatial resolution laser probe 40Ar/39Ar technique with the aim of investigating the complexities of argon behaviour in metamorphic rocks and comparing their thermal histories. Data from a single large phengite grain from the EMC show a range of ages from mid-Jurassic to Upper Cretaceous. These ‘apparent age’ variations are spatially related to both locationwithin the grain and to intragrain microstructure. Modelling of the data shows that the profile formedby the diffusion of an excess argon component into the grain, parallel to the mica cleavage. Profileasymmetry is explained by temporal variations in microstructural development enabling excess argon toenter the grain at different times in different places. The temperatures of the initiation of deformationand the possible time-scales for the deformation can be calculated as a function of cooling rate. Allestimates suggest deformation at greenschist facies, in accord with the observed retrograde mineral assemblage. Absolute temperature estimates for deformation vary by less than 22 °C for different cooling ratesof 10 and 30 °C Ma−1 but vary by 80 °C with different estimates of diffusion parameters. The durationof deformation was for at least 2 Ma at 10 °C Ma−1 or 0.7 Ma at 30 °C Ma−1. Biotites from the IIDKsample record a Permian to Upper Cretaceous age range that correlates with grain size, the smallestgrain sizes yielding the youngest ages. This relationship is best explained by a partial resetting of biotitesduring an Alpine thermal event initiated not more than 70 Ma ago. Modelling of these data suggest thatthe sample never exceeded 300 °C during the Alpine. The profoundly different thermal histories of thetwo units—the EMC recrystallized at 550 °C whilst the IIDK remained below 300 °C—suggests thatthey may not have been juxtaposed until much later than the eclogite facies metamorphism.Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesWood, C. ; Anderson, I. ; Reddy, S. ; Shekleton, P.
Oxford, UK : Blackwell Publishing Ltd
Published 1978Staff ViewISSN: 1471-0528Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: A method for continuous measurement of scalp tissue pH is described. The tissue pH probe was found to be robust and values for tissue pH were close to those for scalp blood pH. Combining the pH and fetal heart rate (FHR) electrodes in the one mechanical assembly facilitated application to the fetal scalp in early labour but the combined assembly electrode was found to have some disadvantages and manufacture of a separate tissue pH electrode is recommended. Continuous monitoring of scalp tissue pH enables closer study of the physiological basis of changes in fetal acid base status and should prove useful to the obstetrician in management of high risk pregnancies during labour.Type of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesReddy, S. M. ; Jones, J. P. ; Lewis, T. J.
[S.l.] : American Institute of Physics (AIP)
Published 1998Staff ViewISSN: 1089-7550Source: AIP Digital ArchiveTopics: PhysicsNotes: It is demonstrated that an AT-cut quartz crystal driven in the thickness-shear-wave mode and typically used as a sensor to monitor the viscoelastic shear-wave properties of a fluid also produce longitudinal pressure waves. Unlike the shear wave, these waves are capable of long-range propagation through the fluid and of reflection at its boundaries, notably at an outer fluid–air interface. They introduce a component into the measured electrical impedance and resonance frequency shift of the crystal, which reflects the setting up of cyclic pressure-wave resonances in the fluid. This has important implications for the practical employment of these crystal as sensors. Under appropriate conditions, as demonstrated for water and n-octane, it is possible to determine the propagating properties of sound waves in a fluid simultaneously with the viscoelastic shear-wave properties. These experiments are supported by an analysis of the appropriate hydrodynamic equations for waves in the crystal–fluid system, which predicts electrical characteristics in close agreement with those found experimentally. © 1998 American Institute of Physics.Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 1745-4522Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Process Engineering, Biotechnology, Nutrition TechnologyNotes: A blend containing hydrogenated fat and a liquid oil was used for preparation of low fat butter spreads. The effects of maltodextrin (MD), skim milk powder (SKMP), guar gum and fully hydrogenated (hardened) vegetable oil on spreadability and phase separation of low fat butter spreads with 30% water were studied. Oil separation in bulk storage and on centrifugation in spreads containing MD, SKMP and guar gum was observed. However, the products prepared by incorporation of 2% hardened oil did not show any oil separation at 25 or 36C. The fat content of the spreads was reduced to 57% by incorporating MD or SKMP along with hardened oil. The spreads showed better spreadability at refrigerated temperatures and better stand up properties at high storage temperatures compared to commercial butter.Type of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 1365-2621Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Process Engineering, Biotechnology, Nutrition TechnologyNotes: Commercial sal fats vary in solidification properties and are unsuitable for use as cocoa butter extenders without further processing. Solvent and melt fractionation processes were used and the fractions were evaluated. One type could be satisfactorily fractionated by solvent or by melt fractionation at 23°C to obtain a stearin (yield 75–85%) of satisfactory quality, but the other could only be satisfactorily processed by melt fractionation at 38°C, removing about 10% stearin. The processing requirement of the fat can be assessed easily by determining the cooling curve.Type of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 1745-4522Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Process Engineering, Biotechnology, Nutrition TechnologyNotes: Nine different brands of commercial vanaspati were analyzed for their various physicochemical characteristics. The Fourier transform infrared spectroscopy spectra showed the characteristic peak at 966/cm corresponding to trans compounds for all the samples examined. The fatty acid composition showed that the trans fatty acid (elaidic acid) content ranged from 5.9 to 30.0%. The other fatty acids were palmitic acid followed by oleic, stearic and linoleic acids. The solid fat content determined by differential scanning calorimetry showed that the samples contained 54–67% solids at 20C, 42–59% at 25C and 0.2–19% at 40C. The slip melting point varied between 39 and 40C. The textural evaluation was carried out using cone penetrometry at 3, 20 and 25C. The fat having the highest trans content showed the highest hardness index. However, there was no direct correlation between the fatty acid composition and consistency.Type of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesPrasad, R. D. G. ; Gillard, P. G. ; Reddy, S. Paddi
College Park, Md. : American Institute of Physics (AIP)
Published 1997Staff ViewISSN: 1089-7690Source: AIP Digital ArchiveTopics: PhysicsChemistry and PharmacologyNotes: Enhancement of the collision-induced absorption (CIA) spectra of the first overtone infrared band of H2 in the H2–Kr and H2–Xe binary mixtures were recorded at 295 K for base densities of H2 in the range 30–57 amagat and for partial densities in the range 50–250 amagat for each of Kr and Xe. The observed spectra consist of single transitions O2(J), Q2(J), and S2(J) corresponding to the selection rule ΔJ=−2, 0, and 2, respectively. Binary, ternary, and quarternary absorption coefficients of the band arising from collisions of the type H2–X, H2–X–X, H2–H2–X,H2–X–X–X, H2–H2–X–X, and H2–H2–H2–X, where X represents Kr or Xe, have been determined from the integrated absorption coefficients of the band. The spectra are interpreted in terms of the quadrupolar induction mechanism and no evidence of the contribution from the isotropic overlap induction mechanism is found unlike in the CIA spectra of the fundamental band of H2 [see S. P. Reddy, in Phenomena Induced by Intermolecular Interactions, edited by G. Birnbaum (Plenum, New York, 1985), p. 129]. Analysis of the absorption profiles of the observed spectra was carried out using the Birnbaum–Cohen line-shape function [G. Birnbaum and E. R. Cohen, Can. J. Phys. 54, 593 (1976)] for individual components of the band and characteristic line shape parameters were determined from the analysis. © 1997 American Institute of Physics.Type of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesREDDY, S. M. ; PANDEY, A. K. ; MELAYAH, D. ; MARMEISSE, R. ; GAY, G.
Oxford, UK : Blackwell Science Ltd
Published 2003Staff ViewISSN: 1365-3040Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: An indoleacetic acid (IAA) up-regulated cDNA, referred to as Pp-C61, was isolated by differential screening of a cDNA library constructed from auxin-treated roots of Pinus pinaster. The Pp-C61 cDNA contains a 1068 bp open reading frame which encodes a polypeptide sharing no homology with known proteins. The N-terminal protein region contains a hydrophobic signal peptide suggesting that Pp-C61 is an extracellular protein. Pp-C61 is present as a single copy in the P. pinaster genome and homologous genes were detected in other gymnosperm and angiosperm trees. Pp-C61 was transcriptionally regulated by auxin with a maximal mRNA accumulation 6 h after treatment. Pp-C61 was up-regulated by IAA concentrations as low as 10− 10m. Up-regulation was found to be a primary (direct) response to auxin. Ethylene and abscisic acid, but not quercetin were able to promote Pp-C61 mRNA accumulation. As a putative extracellular protein, Pp-C61 was postulated to play a role in ectomycorrhiza formation. Pp-C61 was up-regulated in P. pinaster roots following inoculation with ectomycorrhizal fungi. Transcript accumulation was the highest (5.5-fold over control) in the presence of an IAA-overproducing mutant of Hebeloma cylindrosporum; it was less in the presence of wild-type strains of H. cylindrosporum and Rhizopogon roseolus. Pp-C61 transcript also accumulated upon inoculation with a non-mycorrhizal mutant of H. cylindrosporum. These results suggest that Pp-C61 up-regulation corresponds to a defence reaction in response to fungal colonization.Type of Medium: Electronic ResourceURL: