Search Results - (Author, Cooperation:S. P. Wu)

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  1. 1
    J. Qin ; S. P. Wu ; C. J. Creighton ; F. Dai ; X. Xie ; C. M. Cheng ; A. Frolov ; G. Ayala ; X. Lin ; X. H. Feng ; M. M. Ittmann ; S. J. Tsai ; M. J. Tsai ; S. Y. Tsai
    Nature Publishing Group (NPG)
    Published 2012
    Staff View
    Publication Date:
    2012-12-04
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; COUP Transcription Factor II/deficiency/genetics/*metabolism ; Cell Cycle Checkpoints ; Cell Line, Tumor ; *Cell Transformation, Neoplastic ; Disease Models, Animal ; Disease Progression ; Gene Deletion ; Humans ; Male ; Mice ; Neoplasm Metastasis ; PTEN Phosphohydrolase/deficiency/genetics ; Proportional Hazards Models ; Prostate/metabolism/pathology ; Prostatic Neoplasms/*metabolism/*pathology ; *Signal Transduction ; Smad4 Protein/deficiency/genetics/metabolism ; Transforming Growth Factor beta/*antagonists & inhibitors/metabolism
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Cheng, M. C. ; Wu, S. P. ; Chen, L. F. O. ; Chen, S. C. G.
    Springer
    Published 1997
    Staff View
    ISSN:
    1432-2048
    Keywords:
    Key words: Chloroplast ; DNA-binding protein (puri fication) ; Operon ( psaA) ; Spinacia (chloroplast) ; Trans-acting factor ; Transcription
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract. We have previously shown the presence in chloroplasts of sequence-specific DNA-binding proteins that interact specifically with two regions located downstream and upstream from the 5′-transcription start site of the plastid psaA-psaB-rps14 operon. As part of an effort to elucidate the regulatory mechanism of plastid transcription during plant development, we report here the purification and characterization of the chloroplast DNA-binding protein from spinach (Spinacia oleracea L. var. spinosa Ashers et Graeden) leaves that specifically recognizes sequences between positions +64 to +83 relative to the transcription start site. This DNA-binding protein has been highly purified from chloroplasts by using a combination of high-salt extraction, ammonium sulfate precipitation, heparin-agarose chromatography, and sequence-specific DNA-affinity chromatography. The protein exhibited an apparent molecular weight of 59–60 kDa on the basis of gel filtration. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis followed by Southwestern blot analysis further indicated that this DNA-binding protein is dimeric and composed of two ≈31-kDa subunits. We discuss the properties of this protein in relation to the known chloroplast DNA-binding factors for plastid gene expression.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s004250050203

    Articles: DFG German National Licenses