Search Results - (Author, Cooperation:S. J. Morris)

2014-05-30

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Cell Line, Tumor ; Cytarabine/metabolism/pharmacology ; *Drug Resistance, Neoplasm/drug effects/genetics ; Gene Deletion ; Glucuronic Acid/*metabolism ; Glucuronosyltransferase/biosynthesis/*metabolism ; Hedgehog Proteins/*metabolism ; Humans ; Leukemia, Myeloid, Acute/*drug therapy/enzymology/*metabolism/pathology ; Ribavirin/metabolism/pharmacology ; Signal Transduction ; Transcription Factors/antagonists & inhibitors/genetics/*metabolism

2018-03-06

The Society of Nuclear Medicine (SNM)

0022-3123

Medicine

2018-06-30

American Association for the Advancement of Science (AAAS)

2375-2548

Natural Sciences in General

1089-7550

AIP Digital Archive

Physics

Reflectance anisotropy spectroscopy was used to examine the surfaces of AlxGa1−xAs layers grown on GaAs(001) by molecular beam epitaxy, where the Al mole fraction was varied across the whole composition range x=(0.0,0.25,0.50,0.75,1.0). All surfaces were also independently characterized using reflection high-energy electron diffraction, and were found to exhibit a c(4×4) reconstruction. After initial changes in the spectra were observed on depositing very thin layers (≤20 monolayers), in the intermediate thickness range a regime was entered in which strong optical interference effects appeared. These effects are accurately accounted for using a four-media model. For thicker layers (≥8000 monolayers), interference effects were seen to diminish and spectra representative of the surfaces of bulk AlxGa1−xAs were obtained. © 1995 American Institute of Physics.

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

—Antigen-antibody crossed electrophoresis has been applied to the study of rat brain synaptosomes and synaptic vesicles. Several antigens could be visualized. By comparison with previously describéd water-soluble antigens from rat brain, some of the antigens in the synaptosome and the synaptic vesicle preparations were identified; among these were antigens which have been determined as brain-specific. Furthermore, the antisera against the two subcellular fractions were compared with the anti-serum against water-soluble antigens from rat brain.

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

(l) The half-lives of the proteins of various fractions of whole mouse brain increase with increasing insolubility; the supernatant and hypotonic-extractable proteins had half-lives of about 13 days, whereas the membrane proteins solubilized with Triton X-100 and SLS had half-lives of about 18 days. The proteins of the subfractions of synaptosomes had half-lives ranging from 15 to 19 days; those in the cytoplasm had a half-life of 18·3 days, in the membranes, about 17 days and in the synaptic vesicles, 15·6 days.(2) Although the half-life of the synaptic vesicles was not significantly different from that of other synaptosomal subfractions, the vesicles exhibited a different protein pattern on acrylamide gels, an observation which implies that the proteins of the vesicles are qualitatively different from those of other synaptic membranes.(3) The uptake of labelled lysine into the cytoplasm of the synaptosomes of youg mice in vivo was very rapid.(4) The data derived from the relative specific radioactivities of synaptosomal fractions compared with their whole brain analogs support the contention that a sizeable fraction of the synaptosomal cytoplasmic protein was transported to the synapse by axoplasmic flow. The relative specific radioactivities of synaptosomal membrane and synaptic vesicle proteins rose much more quickly than the comparable activities for the cytoplasmic material, and the alternate possibility of synthesis in situ is discussed.

Electronic Resource

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract— Fluorescein isothiocyanate-labelled lectins were used to identify lectin-binding glycoproteins of the chromaffin granule after electrophoresis of the membrane and soluble granule proteins on sodium dodecyl sulphate polyacrylamide slab gels. The glycoprotein nature of all lectin-binding bands was confirmed by staining the gels for carbohydrates, and the specificity of the lectin-binding was demonstrated by hapten sugar inhibition of binding. In samples of granule membrane proteins reduced with dithiothreitol 10 concanavalin A (Con A), 5 wheat germ agglutinin, 8 Ricinus communis agglutinin-60, and 7 Ricinus communis agglutinin-120 (RCA-120) binding glycoproteins were identified. Molecular weights of these glycoproteins varied from 20,000 to 200,000 daltons. All but two of the Con A-binding bands and one of the RCA-120 binding bands appeared to react with more than one lectin, suggesting possible carbohydrate heterogeneity in these membrane glycoproteins. The band identified as dopamine β-hydroxylase reacted most intensely with all four lectin tested, and in the soluble core material this enzyme was the sole significant lectin binding glycoprotein.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

We present in this study reflectance anisotropy spectra also termed reflectance difference spectra for the surfaces of thick Al(x)Ga(1−x)As (x=0.0, 0.25, 0.50, 0.75, 1.00) layers grown on GaAs(001) by molecular beam epitaxy (MBE). Layers ≥2 μm thick were grown in order to minimize the interference effects from the buried interface and to obtain spectra representative of bulk Al(x)Ga(1−x)As surfaces. All surfaces were also independently characterized using reflection high energy electron diffraction (RHEED), and found to exhibit a c(4×4) reconstruction. The reflectance anisotropy spectra were qualitatively similar to one another, but showed clear dependence of energy and magnitude upon the mole fraction x. These spectra can serve as reference for determining stochiometries in MBE growth of Al(x)Ga(1−x)As and probably should be useful for future comparison to metalorganic vapor phase epitaxy (MOVPE) Al(x)Ga(1−x)As growth. © 1995 American Institute of Physics.

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] A bacterial transfer RNA (tRNAFMet) has been obtained in micro-crystalline form. Its probable unit cell dimensions have been deduced from X-ray powder ...

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] A 5.5 Å electron density map of ribonuclease has been obtained by X-ray diffraction using five isomorphous derivatives. One of the cystine residues is identified by means of a chemically modified protein and, using this residue as a starting point, likely positions are found for the other ...

Electronic Resource

1432-0428

Key words Endothelium ; microcirculation ; non-insulin-dependent diabetes mellitus ; skin ; vascular smooth muscle.

Springer Online Journal Archives 1860-2000

Medicine

Summary The mechanisms involved in the pathogenesis of microangiopathy occurring in non-insulin-dependent diabetes mellitus (NIDDM) are unclear. In the present study, blood flow responses to the vasodilators acetylcholine (which acts via the endothelium) and sodium nitroprusside (a smooth muscle relaxant) were evaluated in this patient group. In 14 male patients with NIDDM, treated with either diet alone (n = 6) or diet plus insulin, (mean age 59 years) and 14 age-pair-matched control subjects, forearm skin perfusion following multiple doses of iontophoretically applied 1 % acetylcholine and 0.01 % sodium nitroprusside was recorded by laser Doppler perfusion imaging. Basal skin blood flow was not significantly different in the diabetic group compared with the control group. The following results are expressed as drug-minus-vehicle response. Acetylcholine significantly increased forearm skin perfusion (p 〈 0.001, analysis of variance) in all subjects, but the vasodilatation was attenuated in the patient group compared with control subjects (0.86 ± 0.09 vs 1.36 ± 0.14 arbitrary units of volts (V) respectively, at the fifth measurement point, mean ± SEM, p 〈 0.01). Skin perfusion significantly increased following sodium nitroprusside (p 〈 0.001) but was lower in patients than control subjects (0.12 ± 0.05 vs 0.45 ± 0.11 V, respectively, at the fifth measurement point, p 〈 0.01). These data suggest that endothelial and/or smooth muscle function may be impaired in the skin microcirculation of patients with NIDDM. [Diabetologia (1995) 38: 1337–1344]

Electronic Resource

1432-0428

Endothelium ; microcirculation ; non-insulin-dependent diabetes mellitus ; skin ; vascular smooth muscle

Springer Online Journal Archives 1860-2000

Medicine

Summary The mechanisms involved in the pathogenesis of microangiopathy occurring in non-insulin-dependent diabetes mellitus (NIDDM) are unclear. In the present study, blood flow responses to the vasodilators acetylcholine (which acts via the endothelium) and sodium nitroprusside (a smooth muscle relaxant) were evaluated in this patient group. In 14 male patients with NIDDM, treated with either diet alone (n=6) or diet plus insulin, (mean age 59 years) and 14 age-pair-matched control subjects, forearm skin perfusion following multiple doses of iontophoretically applied 1% acetylcholine and 0.01% sodium nitroprusside was recorded by laser Doppler perfusion imaging. Basal skin blood flow was not significantly different in the diabetic group compared with the control group. The following results are expressed as drug-minus-vehicle response. Acetylcholine significantly increased forearm skin perfusion (p〈0.001, analysis of variance) in all subjects, but the vasodilatation was attenuated in the patient group compared with control subjects (0.86±0.09 vs 1.36±0.14 arbitrary units of volts (V) respectively, at the fifth measurement point, mean ± SEM, p〈0.01). Skin perfusion significantly increased following sodium nitroprusside (p〈0.001) but was lower in patients than control subjects (0.12±0.05 vs 0.45±0.11 V, respectively, at the fifth measurement point, p〈0.01). These data suggest that endothelial and/or smooth muscle function may be impaired in the skin microcirculation of patients with NIDDM.

Electronic Resource

1434-601X

24.30 ; 24.30.CZ ; 25.40.Ep

Springer Online Journal Archives 1860-2000

Physics

Abstract Spin-dipole strength in40Ca has been studied by inelastic scattering of 500-MeV protons and the dipole response in40Ca is compared with the spin-dipole data from the40Ca(p, n) reaction and nonspin-dipole data from photonuclear studies.

Electronic Resource

1741-0444

Artery ; Data processing ; Transfer function models ; Ultrasonic Doppler flowmeters

Springer Online Journal Archives 1860-2000

Biology

Chemistry and Pharmacology

Medicine

Sommaire La thèse décrit une méthode de traitement des ondes de vitssse d'arrivée/sortie du sang dans les artères fémorales, grâce à des débitmètres Doppler à ultrasons. Elle commente quelques cas normaux et débuts de maladies, et l'on constate qu'un affichage illustré des racines d'équation du second degré fournit une évaluation quantitative simple du progrès et du traitement de la maladie.

Zusammenfassung Die Arbeit beschreibt ein Verfahren zur Verarbeitung durch Ultraschall-Doppler-Durchflußmesser gewonnener Eingangs/Ausgangs-Durchblutungsgeschwindigkeitswellenformen für Schenkelarterien. Es werden normale und erkrankte sowie Fälle im Anfangsstadium der Erkrankung untersucht, und es wird gezeigt, daß eine bildliche Darstellung der Wurzeln einer Quadratgleichung eine einfache quantitative Bewertung des Fortschreitens und der Behandlung von Erkrankungen ermöglicht.

Abstract The paper describes a method for processing input/output blood velocity waveforms for femoral arteries obtained from ultrasonic Doppler flowmeters. Normal, diseased and early cases of disease are discussed, and it is shown that a pictorial display of the roots of a quadratic equation provide a simple quantitative assessment of progress and treatment of disease

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