Search Results - (Author, Cooperation:S. J. Dunn)

2014-06-07

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Animals ; Cell Culture Techniques ; Computational Biology ; Embryonic Stem Cells/*metabolism ; *Gene Expression Regulation ; *Gene Regulatory Networks ; Mice ; Pluripotent Stem Cells/*metabolism ; Transcription Factors/genetics/*metabolism

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary Rhesus rotavirus (RRV) VP4 trypsin cleavage product VP5(1)*, a truncated form of VP5*, was expressed in baculovirus and found by immunoprecipitation to be antigenically similar to VP5* on the virion. Immunization of mice with VP5(1)* elicited neutralizing antibody that was found to be cross-reactive with viruses representing P genotypes 1, 3, 4, 6, 7, and 8. Baculovirus expressed trypsin cleavage products, VP8* (amino acids 1–246) and VP5(1)* (amino acids 247–474), were tested for their ability to elicit a protective response in a murine model of passive protection. These results were compared to those obtained with baculovirus expressed RRV VP4. Dams immunized with baculovirus expressed RRV VP4 gave birth to pups protected from RRV virus challenge. Neither VP5(1)* nor VP8* was as effective at generating protective immunity as full length VP4. However, antibody to VP5(1)* was more effective than antibody to VP8* at mediating protection even though the neutralizing antibody titers as measured by hemagglutination inhibition and focus reduction neutralization were similar.

Electronic Resource

1573-5036

Differential response ; Genetic variability ; Micronutrients ; Soybean [Glycine max. (L.) Merr.]

Springer Online Journal Archives 1860-2000

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Summary A study was conducted to evaluate the effect of manganese on accumulation of Zn, Fe, Cu, and B by different soybean genotypes under varying soil pH conditions, in view of determining tolerance towards extremes in soil pH and mineral toxicities. Enon sandy loam soil was used for the investigation in a greenhouse. Manganese rates were 0, 10, and 20 kg/ha. The pH levels were 5.3, 6.3, and 7.0. The soybean genotypes tested were PI 159319, PI 324924, PI 960895, and L-76-0132. Results indicate that Zn concentration in the genotypes leaf and seed was not affected by Mn application whereas the Cu concentration in the leaf was affected. In general, there was an increase in concentration of Fe in the genotypes seed with Mn application. Boron concentration in the genotypes leaf and seed was unaffected by Mn application. The effect of soil pH on Zn, Fe, Cu, and B in the genotypes tissue was significant. This did not result in toxic levels of the elements in the tissues.

Electronic Resource

1573-5036

manganese ; soybean

Springer Online Journal Archives 1860-2000

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Abstract A study was conducted to evaluated response of four soybean genotypes to different soil pH conditions, and rates of manganese application. Enon sandy loam soil was used for the investigation in a greenhouse. Manganese rates were 0, 10, and 20 Kg Mn ha. The pH levels were 5.3, 6.3, and 7.0. The genotypes tested were PI 159319, PI 324924, PI 960895, and L-76-0132. Genotype PI 960895 gave the highest yield whereas I-76-0132 gave the lowest yield. Genotype PI 324924 showed normal plant growth and higher yield at pH 5.3 indicating its tolerance to low soil pH. Soil pH 5.3 resulted in highest concentration of Mn in the leaves. Relatively lower seed yield of PI 159319 at pH 5.3 was due to intolerance to low soil pH. Genotypes PI 960895 and L-76-0132 gave similar seed yields at all pH levels. Genotypes PI 159319 responded to Mn addition while the others did not. The data suggest differential response by soybean genotypes to soil pH and Mn addition.

Electronic Resource