Search Results - (Author, Cooperation:S. Huet)

2013-08-27

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Cell Cycle/genetics ; Cell Cycle Proteins/metabolism ; Chromatids/genetics/metabolism ; Chromatin/*chemistry/genetics/*metabolism ; Chromosomal Proteins, Non-Histone/metabolism ; *Chromosome Segregation/genetics ; Chromosomes, Mammalian/chemistry/genetics/metabolism ; DNA/genetics/metabolism ; DNA-Binding Proteins/deficiency/genetics/metabolism ; Embryonic Development/genetics ; Endopeptidases/metabolism ; Gene Expression Regulation/genetics ; Genes, myc/genetics ; Interphase ; Mice ; Mitosis ; Prophase ; Proteins/genetics/*metabolism ; Separase

2018-08-24

American Society of Hematology (ASH)

0006-4971

1528-0020

Biology

Medicine

Myeloid Neoplasia

1089-7550

AIP Digital Archive

Physics

In this work we present a detailed structural characterization by Raman spectroscopy of hydrogenated amorphous silicon (a-Si:H) and of nanostructured silicon (ns-Si:H) thin films grown in radio-frequency plasma. The ns-Si:H thin films, also called polymorphous Si thin films, consist of a two-phase mixture of amorphous and ordered Si. The Raman spectra were measured at increasing laser intensities. Very low laser power densities (∼1 kW/cm2) were used to thoroughly analyze the structure of as-deposited thin films. Higher Raman laser powers were found to induce the crystallization of the films, which was characterized by the appearance of a sharp peak around 500 cm−1. This was attained faster in the ns-Si:H than in the conventional a-Si:H thin films because the silicon-ordered particles cause a heterogeneous nucleation process in which they act as seeds for crystallization. The laser power densities for film crystallization, crystal size, and surface temperature were determined from this Raman analysis. The validity and application ranges of the different models that can be used to calculate these parameters are critically discussed. © 2001 American Institute of Physics.

Electronic Resource

1089-7550

AIP Digital Archive

Physics

In this study, we present a detailed structural characterization by means of transmission electron microscopy and Raman spectroscopy of polymorphous silicon (pm-Si:H) thin films deposited using radio-frequency dust-forming plasmas of SiH4 diluted in Ar. Square-wave modulation of the plasma and gas temperature was varied to obtain films with different nanostructures. Transmission electron microscopy and electron diffraction have shown the presence of Si crystallites of around 2 nm in the pm-Si:H films, which are related to the nanoparticles formed in the plasma gas phase coming from their different growth stages, named particle nucleation and coagulation. Raman scattering has proved the role of the film nanostructure in the crystallization process induced "in situ" by laser heating. © 2001 American Institute of Physics.

Electronic Resource

1077-3118

AIP Digital Archive

Physics

A method for the detection of dust particles occurring in silane–argon gas mixture plasmas is presented. It is based on the spectral analysis of the radio-frequency current. The amplitudes of the fundamental (13.56 MHz) and second harmonics (40.68 MHz) are very sensitive to the presence of the earlier nanoparticles when their size is in the range of 2–3 nm even if their influence on the capacitive character of the impedance is negligible. This method is nonperturbative, with a temporal resolution in the microsecond range, very easy to implement, and can thus be used for industrial reactors. © 2001 American Institute of Physics.

Electronic Resource

1600-0536

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

0248-4900

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

0248-4900

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

0248-4900

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

1279-8509

Antigen presenting cells ; Human bone marrow ; In vitro production

Springer Online Journal Archives 1860-2000

Medicine

Abstract In the prospect of producing autologous antigen presenting cells (APC) to actively immunize patients with cancer against their own tumor we were interested in the in vitro generation of MC and/or dendritic cells. We observed that the best yielding in CD14+ cells was obtained by adding SCF and GM-CSF into RPMI 1640 completed medium and by using Teflon bags as culture-containers. The others growth factors tested (LIF, IL3 and M-CSF) were useless in term of production of macrophages. After a month of culture we usually obtained an average of 80% of CD14, CD33, CD64, CD11a, CD11b, CD11c and HLA-DR positive cells expressing the MGG staining phenotype of MC. For DC the best association of growth factors combined GM-CSF, IL4 and SCF. Hence we could obtained at least 60% of CD1a+, CD14-, CD54+, CD58+, CD80+ and HLA DR+ dendritic cells.

Electronic Resource

1573-4978

HMBA ; Burkitts Lymphoma ; c-myc ; tyrosine phosphorylation

Springer Online Journal Archives 1860-2000

Biology

Abstract Hexamethylenbisacetamide (HMBA) can induce the Burkitt lymphoma Raji cells to enter the differentiation process as evidenced by the decrease of HLA-DR antigens. This event is preceded by a decrease of c-myc expression and of the phosphorylation of cellular proteins, due toeither a decrease of tyrosine protein kinase activity or an increase of tyrosine phosphatase activity. These three events form a sequence and are part of the genetic program for differentiation and growth though they may not be causally related.

Electronic Resource