Search Results - (Author, Cooperation:S. Fukuhara)

2012-10-16

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Base Sequence ; Biocatalysis ; Catalytic Domain ; Crystallography, X-Ray ; DNA Transposable Elements/genetics ; Drosophila Proteins/*chemistry/*metabolism ; Drosophila melanogaster/*enzymology/genetics ; Endoribonucleases/*chemistry/*metabolism ; Gene Silencing ; Models, Molecular ; Molecular Sequence Data ; Protein Conformation ; RNA, Small Interfering/biosynthesis/chemistry/genetics/*metabolism ; Structure-Activity Relationship

0005-2728

L protein ; Low molecular mass protein ; Photosystem II ; Q"A ; Reconstitution ; [abr] Bistris; bis (2-hydroxyethyl)iminotris(hydroxymethyl)methane ; [abr] Chl; chlorophyll ; [abr] DCMU; 3-(3,4-dichlorophenyl)-1,1-dimethylurea ; [abr] DCPIP; 2,6-dichloro-phenolindophenol ; [abr] DM; n-dodecyl β-d-maltoside ; [abr] FeCN; potassium ferricyanide ; [abr] HPLC; high-performance liquid chromatography ; [abr] LHCP; light harvesting chlorophyll a/b proteins ; [abr] Mes; 2-(N-morpholino)ethanesulfonic acid ; [abr] Mops; 3-(N-morpholino)propanesulfonic acid ; [abr] OGP; n-octyl β-d-glucopyranoside ; [abr] ORF; open reading frame ; [abr] OTG; n-octyl β-d-thioglucopyranoside ; [abr] P680; primary donor chlorophyll a of Photosystem II ; [abr] PQA; plastoquinone A-9 ; [abr] PS II; Photosystem II ; [abr] Pheo a; pheophytin a ; [abr] Q"A; primary quinone acceptor of Photosystem II ; [abr] Q"B; secondary quinone acceptor of Photosystem II ; [abr] RC; reaction center complex of Photosystem II ; [abr] SDS-PAGE; SDS polyacrylamide gel electrophoresis ; [abr] Tricine; tris(hydroxymethyl)methylglycine ; [abr] Z; primary electron donor to P680 ; [abr] β-Car; β-carotene ; psbL gene product

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Medicine

Physics

Electronic Resource

0039-6028

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Physics

Electronic Resource

0039-6028

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Physics

Electronic Resource

0039-6028

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Physics

Electronic Resource

0039-6028

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Physics

Electronic Resource

0167-9317

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Electrical Engineering, Measurement and Control Technology

Electronic Resource

0167-9317

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Electrical Engineering, Measurement and Control Technology

Electronic Resource

1432-0584

Immune thrombocytopenic purpura Microparticle ; Anticardiolipin antibody P-selectin ; β 2-glycoprotein I

Springer Online Journal Archives 1860-2000

Medicine

Abstract We investigated the association ofβ 2-glycoprotein I and P-selectin with platelet-derived microparticles in 48 patients with immune thrombocytopenic purpura and 20 normal controls using two-color flow cytometric analysis. In addition, anticardiolipin antibodies were detected by an enzyme-linked immunosorbent assay. Platelet microparticles from the patients showed a higher positivity forβ 2-glycoprotein I than those from the normal controls (23.1±15.4% vs. 5.3±3.1%, p〈0.01), but this positivity was not related to the presence of platelet-associated IgG or to the severity of thrombocytopenia. In the 18 patients with more than 20% P-selectin-positive microparticles,β 2-glycoprotein I positivity was significantly higher than in the 30 patients with less than 20% P-selectin-positive microparticles (37.1±20.5% vs. 21.5±17.3%, p〈0.01). In addition, anticardiolipin antibodies were detected in eight patients, and they had a significantly higher level ofβ 2-glycoprotein I-positive microparticles than the patients without such antibodies (42.0±22.9% vs. 22.6±18.9%, p〈0.05). Our results suggest that anticardiolipin antibodies activate platelets in immune throm bocytopenic purpura and cause the generation of microparticles rich inβ 2-glycoprotein I and P-selectin. These microparticles may then act to regulate coagulation abnormalities in patients with anticardiolipin antibodies.

Electronic Resource

0165-4608

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Medicine

Electronic Resource

1432-069X

Springer Online Journal Archives 1860-2000

Medicine

Zusammenfassung Bei zwei Patienten mit Leishmaniasis cutanea der alten Welt konnten floride und später ein Restherd zur elektronenmikroskopischen Untersuchungen excidiert und dabei folgendes beobachtet werden: 1. Im floriden Herd zeigte sich eine pseudocarcinomatöse follikuläre Epidermisproliferation. 2. In histiocytären Nestern lassen sich Macrophagen mit einer bis drei Leishmaniazellen nachweisen. Die Parasiten sind nicht immer eindeutig von einer Wirtszellmembran umgeben. 3. Die intrazellulären Leishmania-Zellen sind mit einem nach außen offenen Geißelapparat versehen. Deswegen ist es richtig von einer kryptomastigoten Form der Flagelle zu sprechen. Der Kinetoplast zeigt direkten Übergang zum Mitochondrium. Die Anzahl der Periplastfibrillen oder Tubuli betrug 95–97, geringer als bei den anderen Leishmaniaarten. Dennoch läßt sich im Vergleich mit der Literatur kein weiteres eindeutiges morphologisches Unterscheidungsmerkmal zwischen der Leishmania tropica und den anderen Formen erkennen. 4. Im späten Restherd einer früher gesicherten Orientbeule konnten jetzt keine als virulent anzusehenden Parasiten beobachtet werden. Die Macrophagen enthielten dafür große Vacuolen mit lamellären Residualkörpern. Diese werden als Reste der Parasiten angesehen.

Summary On two patients suffering from Leishmaniasis cutanea from the old world florid and later a residual lesion could be removed for electron-mircroscopic examination, and the following was found: 1. A pseudocarcinomatous follicular epidermal proliferation in the florid lesion. 2. Macrophages with one to three Leishmania cells can be proved in histiocytes nests. The parasites are not always clearly surrounded by host cell membrane. 3. The endocellular Leishmania cells have a ciliary system which is open on the outside. It is, therefore, correct to speak of a cryptomastigotic shape of the flagellum. The kinetoplast shows direct transition to mitochondria. The number of periplast fibrillae or tubuli amounted to 95–97 which is less than in other types of Leishmania. Yet no further morphological distinctive feature between Leishmania tropica and the other types is discernible when a comparison with the pertinent literature is made. 4. No parasites to be considered virulent were found now in the late residual focus of previously secured cutaneous Leishmaniasis. The macrophages, on the other hand, contained big vacuoles with lamellar residual substances. These are regarded as rests of the parasites.

Electronic Resource

1432-0584

Key words Immune thrombocytopenic purpura ; Microparticle ; Anticardiolipin antibody ; P-selectin ; β2-glycoprotein I

Springer Online Journal Archives 1860-2000

Medicine

Abstract  We investigated the association of β2-glycoprotein I and P-selectin with platelet-derived microparticles in 48 patients with immune thrombocytopenic purpura and 20 normal controls using two-color flow cytometric analysis. In addition, anticardiolipin antibodies were detected by an enzyme-linked immunosorbent assay. Platelet microparticles from the patients showed a higher positivity for β2-glycoprotein I than those from the normal controls (23.1±15.4% vs. 5.3±3.1%, p〈0.01), but this positivity was not related to the presence of platelet-associated IgG or to the severity of thrombocytopenia. In the 18 patients with more than 20% P-selectin-positive microparticles, β2-glycoprotein I positivity was significantly higher than in the 30 patients with less than 20% P-selectin-positive microparticles (37.1±20.5% vs. 21.5±17.3%, p〈0.01). In addition, anticardiolipin antibodies were detected in eight patients, and they had a significantly higher level of β2-glycoprotein I-positive microparticles than the patients without such antibodies (42.0±22.9% vs. 22.6±18.9%, p〈0.05). Our results suggest that anticardiolipin antibodies activate platelets in immune thrombocytopenic purpura and cause the generation of microparticles rich in β2-glycoprotein I and P-selectin. These microparticles may then act to regulate coagulation abnormalities in patients with anticardiolipin antibodies.

Electronic Resource

1432-069X

Springer Online Journal Archives 1860-2000

Medicine

Zusammenfassung Bei einem Jungen aus Togo wurde ein therapieresistentes Rhinosclerom über 3 Jahre beobachtet. Histologisch und elektronenmikroskopisch konnte das spezifische Granulom untersucht werden. Die Plasmazellen bilden im rauhen endoplasmatischen Reticulum fibrilläres Material oder tropfige Russel-Körper. Deren strukturelle Bildung wird diskutiert. Die Mikulicz-Zellen und die Klebsiella rhinoscleromatis werden beschrieben. Die Zellen werden als (histiocytäre) Makrophagen angesehen. Die Erreger sind außen von einer fimbrienartigen, strahlig aufsitzenden Substanz in den Vacuolen der Zellen vernetzt. Dieses Material könnte den antigenen Reiz zur Bildung der Russel-Körper darstellen. Die Russel-Körper entsprechen vielleicht vergeblich gebildetem und gleichsam steckengebliebenem Immunmaterial.

Summary A boy from Togo with a resistent Rhinoscleroma was observed. The specific granuloma was examinated by histological and electron microscopic methods. The plasma cells form fibrillary material or guttate Russel bodies in the rough endoplasmatic reticulum. The morphology of Mikulicz-cells and Klebsiella rhinoscleromatis is described. The cells are supposed to be (histiocytic) macrophages. The surface of the germs is coated with a fimbrialike radiate substance which in the vacuoles of the cells is crosslinked. This material could be the eliciting antigen for the formation of Russel bodies. These bodies then may represent immune substances bound within the cell.

Electronic Resource

1573-2649

Cross-cultural studies ; health assessment ; MOS SF-36 ; quality of life ; questionnaire translation

Springer Online Journal Archives 1860-2000

Medicine

Abstract The International Quality of Life Assesment (IQOLA) Project is a 4-year project to translate and adapt the widely used MOS SF-36 Health Survey Questionnaire in up to 15 countries and validate, norm, and document the new translations as required for their use in international studies of health outcomes. In addition to the eight-scale SF-36 health profile, the project will also validate psychometrically based physical and mental health summary scores, as well as health utility indexes incorporating SF-36 scales for use in cost-utility studies.

Electronic Resource

1435-232X

Key words D-- phenotype ; CE-D-CE hybrid gene ; RH genes ; D1S80 locus ; DNA fingerprints ; Gametogenesis

Springer Online Journal Archives 1860-2000

Biology

Medicine

Abstract In a family study of a Japanese propositus with the D-- phenotype, the serological data of her D-- phenotype and those of her parents were discrepant. Gene analysis of the propositus showed a gross deletion of the RHCE gene and a new rearrangement of RHCE to yield the CE-D-CE hybrid. It was demonstrated that the hybrid CE-D-CE gene consisted of exon 1 from the RHCE gene, followed by exons 3 to 7 from the RHD gene and exons 8 to 10 from the RHCE gene. However, whether or not exon 2 of the RHD or the RHCE gene was contained in the CE-D-CE gene remained unclear. Moreover, spacer analysis between both RH genes and the family study suggested that the D-- gene complex from the paternal and maternal sides consisted of only the CE-D-CE hybrid gene and a single RHD gene, respectively. For the purpose of confirming the parent-child relationship, a paternity test using DNA fingerprint and polymerase chain reaction (PCR) analysis at the D1S80 locus were performed. DNA fingerprints with two kinds of DNA minisatellite probes (33.15 and 33.6) confirmed that the parent-child relationship in the D-- propositus was compatible. However, in the present case, at the D1S80 locus, the PCR product derived from the mother was lacking, thereby negating a parent-child relationship. It is probable that the RH genes and D1S80 locus exist in close proximity, because they are situated in chromosomes 1p 34.3–36.1 and 1p 36.1–36.3, respectively. These data suggested that at the stage of gametogenesis, both the RHCE gene and the D1S80 locus from the maternal side may have been deleted, thereby producing the D-- gene complex.

Electronic Resource