Search Results - (Author, Cooperation:S. Bonnefoy)

Showing 1 - 7 results of 7, query time: 0.15s Refine Results
  1. 1
    J. Aleksic ; S. Ansoldi ; L. A. Antonelli ; P. Antoranz ; A. Babic ; P. Bangale ; J. A. Barrio ; J. Becerra Gonzalez ; W. Bednarek ; E. Bernardini ; B. Biasuzzi ; A. Biland ; O. Blanch ; S. Bonnefoy ; G. Bonnoli ; F. Borracci ; T. Bretz ; E. Carmona ; A. Carosi ; P. Colin ; E. Colombo ; J. L. Contreras ; J. Cortina ; S. Covino ; P. Da Vela ; F. Dazzi ; A. De Angelis ; G. De Caneva ; B. De Lotto ; E. de Ona Wilhelmi ; C. Delgado Mendez ; D. Dominis Prester ; D. Dorner ; M. Doro ; S. Einecke ; D. Eisenacher ; D. Elsaesser ; M. V. Fonseca ; L. Font ; K. Frantzen ; C. Fruck ; D. Galindo ; R. J. Garcia Lopez ; M. Garczarczyk ; D. Garrido Terrats ; M. Gaug ; N. Godinovic ; A. Gonzalez Munoz ; S. R. Gozzini ; D. Hadasch ; Y. Hanabata ; M. Hayashida ; J. Herrera ; D. Hildebrand ; J. Hose ; D. Hrupec ; W. Idec ; V. Kadenius ; H. Kellermann ; K. Kodani ; Y. Konno ; J. Krause ; H. Kubo ; J. Kushida ; A. La Barbera ; D. Lelas ; N. Lewandowska ; E. Lindfors ; S. Lombardi ; F. Longo ; M. Lopez ; R. Lopez-Coto ; A. Lopez-Oramas ; E. Lorenz ; I. Lozano ; M. Makariev ; K. Mallot ; G. Maneva ; N. Mankuzhiyil ; K. Mannheim ; L. Maraschi ; B. Marcote ; M. Mariotti ; M. Martinez ; D. Mazin ; U. Menzel ; J. M. Miranda ; R. Mirzoyan ; A. Moralejo ; P. Munar-Adrover ; D. Nakajima ; A. Niedzwiecki ; K. Nilsson ; K. Nishijima ; K. Noda ; R. Orito ; A. Overkemping ; S. Paiano ; M. Palatiello ; D. Paneque ; R. Paoletti ; J. M. Paredes ; X. Paredes-Fortuny ; M. Persic ; J. Poutanen ; P. G. Prada Moroni ; E. Prandini ; I. Puljak ; R. Reinthal ; W. Rhode ; M. Ribo ; J. Rico ; J. Rodriguez Garcia ; S. Rugamer ; T. Saito ; K. Saito ; K. Satalecka ; V. Scalzotto ; V. Scapin ; C. Schultz ; T. Schweizer ; S. N. Shore ; A. Sillanpaa ; J. Sitarek ; I. Snidaric ; D. Sobczynska ; F. Spanier ; V. Stamatescu ; A. Stamerra ; T. Steinbring ; J. Storz ; M. Strzys ; L. Takalo ; H. Takami ; F. Tavecchio ; P. Temnikov ; T. Terzic ; D. Tescaro ; M. Teshima ; J. Thaele ; O. Tibolla ; D. F. Torres ; T. Toyama ; A. Treves ; M. Uellenbeck ; P. Vogler ; R. Zanin ; M. Kadler ; R. Schulz ; E. Ros ; U. Bach ; F. Krauss ; J. Wilms
    American Association for the Advancement of Science (AAAS)
    Published 2014
    Staff View
    Publication Date:
    2014-11-08
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Bischoff, E. ; Guillotte, M. ; Mercereau-Puijalon, O. ; Bonnefoy, S.

    Oxford, UK : Blackwell Science Ltd
    Published 2000
    Staff View
    ISSN:
    1365-2958
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Medicine
    Notes:
    Four large multigene families have been described in Plasmodium falciparum malaria parasites (var, rif, stevor and Pf60). var and rif genes code for erythrocyte surface proteins and undergo clonal antigenic variation. We report here the characterization of the first Pf60 gene. The 6.1 gene is constitutively expressed by all mature blood stages and codes for a protein located within the nucleus. It has a single copy, 7-exon, 5′ domain, separated by an internal stop codon from a 3′ domain that presents a high homology with var exon II. Double-site immunoassay and P. falciparum transient transfection using the reporter luciferase gene demonstrated translation through the internal ochre codon. The 6.1 N-terminal domain has no homology with any protein described to date. Sequence analysis identified a leucine zipper and a putative nuclear localization signal and showed a high probability for coiled coils. Evidence for N-terminal coiled coil-mediated protein interactions was obtained. This identifies the 6.1 protein as a novel nuclear protein. These data show that the Pf60 and var genes form a superfamily with a common 3′ domain, possibly involved in regulating homo- or heteromeric interactions.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1046/j.1365-2958.2000.01788.x

    Articles: DFG German National Licenses
  3. 3
    DIEZ, M. ; GUILLOTTE, M. ; LE SCANF, C. ; CONTAMIN, H. ; DAVID, P. ; COOKE, B. ; MERCERAU-PUIJALON, O. ; BONNEFOY, S.

    Oxford, UK : Blackwell Science Inc
    Published 2005
    Staff View
    ISSN:
    1550-7408
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    Pf155/RESA (Ring Infected Erythrocyte Surface Antigen), is a Plasmodium falciparum protein discharged by the dense granules of merozoites during invasion and exported to the inner face of the erythrocyte membrane, where it interacts with spectrin in the young stages. Chromosome 1 subtelomeric deletion, eliminating amongst others the resa gene, may occur during adaptation of parasite isolates to in vitro culture. This is accompanied by erythrocyte membrane modifications of the red blood cell, such as increased adhesion and effect on membrane rigidity. It has been proposed that RESA was likely to contribute to these functional and rheological modifications. To assess this, we have constructed resa knock-out parasites in the FUP/CB strain. This results in negativating the erythrocyte membrane immunofluorescence of glutaraldehyde-fixed red blood cells (EMIF), indicating that RESA is critical for EMIF reactivity of hyperimmune human sera. Phenotypic and functional analysis of resa k.o. parasites indicated that loss of RESA expression neither affects membrane rigidity nor CD36 binding under flow conditions. Furthermore, infection of Saimiri sciureus monkeys showed that resa gene deletion does not account on its own for the greater adaptation and parasite virulence in this model.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1550-7408.2005.05202003_3_9.x

    Articles: DFG German National Licenses
  4. 4
    BONNEFOY, S. ; DIEZ, M. ; PENDYALA, P. R. ; BLANC, C. ; GUILLOTTE, M. ; MERCEREAU-PUIJALON, O.

    Oxford, UK : Blackwell Science Inc
    Published 2005
    Staff View
    ISSN:
    1550-7408
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Notes:
    In the past few years, genetic manipulation of Plasmodium falciparum has allowed functional studies of many parasite proteins. However, a careful interpretation of gene manipulation results is required, as chromosomal events may occur in vitro independently of the experimental modifications (gene invalidation, overexpression …), and because of the intrinsic mutagenic potential of genetic manipulation, or pleiotropic consequences related to the insertion of exogenous sequences. To ensure that mutant phenotypes arise from the planned mutations, it is necessary to obtain revertants re-expressing the wild-type gene. On this line, we have developed a positive/negative selection strategy allowing in a first step, gene targeting, then excision of the resistance marker. We converted the Toxoplasma gondii DHFR/TS into a tri-functional enzyme following fusion with the Herpes virus type I thymidine kinase (HSV-TK1). Positive selection corresponds to pyrimethamine resistant development, while negative selection acts in presence of the pro-drogue ganciclovir. We used this tool to study RESA, a dense granule protein that associates with spectrin following invasion. Resa gene invalidation was obtained using a targeting plasmid allowing expression of the fusion DHFR/TS/HSV-TK1, and molecular and phenotypic analyses of mutant parasites were performed. Next, wild-type resa gene expression was restored after excision of all the exogenous sequences following phosphorylation of ganciclovir by the HSV thymidine kinase into a toxic metabolite. Phenotypic studies of revertants confirm some functions attributed to RESA protein, and validate the positive/negative strategy in this reverse genetic approach, a useful tool for development of “hit and run” mutagenesis in P. falciparum.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1550-7408.2005.05202003_3_3.x

    Articles: DFG German National Licenses
  5. 5
    Staff View
    ISSN:
    0264-410X
    Keywords:
    Plasmodium falciparum ; heat shock protein 90 ; squirrel monkeys ; vaccination
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0264-410X(94)90008-6

    Articles: DFG German National Licenses
  6. 6
    Staff View
    ISSN:
    0166-6851
    Keywords:
    ATP binding protein ; Heat shock protein 90 ; Parasitophorous vacuole ; Phylogenetic tree ; Plasmodium falciparum ; [abr] ER; endoplasmic reticulum ; [abr] PFGE; pulse field gel electrophoresis ; [abr] grp; glucose regulated protein ; [abr] hsp; heat shock protein ; [abr] mAb; monoclonal antibody ; cDNA sequence
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0166-6851(94)90105-8

    Articles: DFG German National Licenses
  7. 7
    Staff View
    ISSN:
    0166-6851
    Keywords:
    Babesia ; Invasion ; Malaria ; Merozoite ; Multigene family ; Polymorphism
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0166-6851(94)90167-8

    Articles: DFG German National Licenses