Search Results - (Author, Cooperation:R. Waugh)

Showing 1 - 20 results of 50, query time: 0.35s Refine Results
  1. 1
    Staff View
    Publication Date:
    2012-10-19
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Alternative Splicing/genetics ; Codon, Nonsense/genetics ; Crops, Agricultural/genetics ; Evolution, Molecular ; Gene Expression Regulation, Plant ; Genes, Plant/genetics ; Genome, Plant/*genetics ; Genomics ; Hordeum/classification/*genetics ; Molecular Sequence Annotation ; Physical Chromosome Mapping ; Polymorphism, Single Nucleotide/genetics ; Repetitive Sequences, Nucleic Acid/genetics ; *Sequence Analysis, DNA ; Transcriptome/genetics
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    Publication Date:
    2013-07-05
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Acclimatization/genetics ; Agriculture/economics/*methods/*trends ; Biodiversity ; Biological Specimen Banks ; Breeding ; Crops, Agricultural/genetics ; Food Supply/*statistics & numerical data ; Genes, Plant ; Humans ; Phenotype ; Seeds/genetics
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  3. 3
    X. Xu ; S. Pan ; S. Cheng ; B. Zhang ; D. Mu ; P. Ni ; G. Zhang ; S. Yang ; R. Li ; J. Wang ; G. Orjeda ; F. Guzman ; M. Torres ; R. Lozano ; O. Ponce ; D. Martinez ; G. De la Cruz ; S. K. Chakrabarti ; V. U. Patil ; K. G. Skryabin ; B. B. Kuznetsov ; N. V. Ravin ; T. V. Kolganova ; A. V. Beletsky ; A. V. Mardanov ; A. Di Genova ; D. M. Bolser ; D. M. Martin ; G. Li ; Y. Yang ; H. Kuang ; Q. Hu ; X. Xiong ; G. J. Bishop ; B. Sagredo ; N. Mejia ; W. Zagorski ; R. Gromadka ; J. Gawor ; P. Szczesny ; S. Huang ; Z. Zhang ; C. Liang ; J. He ; Y. Li ; Y. He ; J. Xu ; Y. Zhang ; B. Xie ; Y. Du ; D. Qu ; M. Bonierbale ; M. Ghislain ; R. Herrera Mdel ; G. Giuliano ; M. Pietrella ; G. Perrotta ; P. Facella ; K. O'Brien ; S. E. Feingold ; L. E. Barreiro ; G. A. Massa ; L. Diambra ; B. R. Whitty ; B. Vaillancourt ; H. Lin ; A. N. Massa ; M. Geoffroy ; S. Lundback ; D. DellaPenna ; C. R. Buell ; S. K. Sharma ; D. F. Marshall ; R. Waugh ; G. J. Bryan ; M. Destefanis ; I. Nagy ; D. Milbourne ; S. J. Thomson ; M. Fiers ; J. M. Jacobs ; K. L. Nielsen ; M. Sonderkaer ; M. Iovene ; G. A. Torres ; J. Jiang ; R. E. Veilleux ; C. W. Bachem ; J. de Boer ; T. Borm ; B. Kloosterman ; H. van Eck ; E. Datema ; B. Hekkert ; A. Goverse ; R. C. van Ham ; R. G. Visser
    Nature Publishing Group (NPG)
    Published 2011
    Staff View
    Publication Date:
    2011-07-12
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Evolution, Molecular ; Gene Duplication ; Gene Expression Regulation, Plant ; Genes, Plant/genetics ; Genetic Variation ; Genome, Plant/*genetics ; *Genomics ; Haplotypes/genetics ; Heterozygote ; Homozygote ; Immunity, Innate ; Inbreeding ; Molecular Sequence Annotation ; Molecular Sequence Data ; Plant Diseases/genetics ; Ploidies ; Solanum tuberosum/*genetics/physiology
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  4. 4
    Wu, L.-F. ; Mandrand-Berthelot, M.-A. ; Waugh, R. ; Edmonds, C. J. ; Holt, S. E. ; Boxer, D. H.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1989
    Staff View
    ISSN:
    1365-2958
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Biology
    Medicine
    Notes:
    Hydrogenase activity and other hydrogenase-related functions can be restored to hydC mutants by the specific addition of nickel salts to the growth medium. These mutants are defective in all three hydrogenase isoenzymes and the restoration is dependent upon protein synthesis. The cellular nickel content of the mutant when grown in LB medium is less than 1% of that of the parental strain. Partial suppression of the hydrogenase phenotype of hydC mutants occurs when growth takes place in a different medium. This correlates with an increased cellular nickel content. The phenotype of the mutant is also fully suppressed by growth in media of very low magnesium content. Such media facilitate nickel uptake via the magnesium transport system, which leads to the acquisition of a normal cellular nickel content. Mutations in the fnr gene, which encodes a transcriptional regulator for several anaerobically expressed enzymes, abolishes hydC expression and gives rise to a defective hydrogenase phenotype. The hydrogenase phenotype of fnr is closely similar to that of hydC in all respects examined. The hydrogenase activity of fnr strains can be restored by the presence of a functional hydC gene on a multicopy plasmid. The hydrogenase phenotype of fnr strains therefore arises indirectly via suppression of hydC, which leads to a low cellular nickel content. Nickel has no influence on fumarate reductase or nitrate reductase activities in fnr strains. The hydrogen-metabolism phenotype of fnr strains is, therefore, dependent upon their ability to acquire nickel from growth media. It is likely that hydC encodes a specific transport system for nickel.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1365-2958.1989.tb00156.x

    Articles: DFG German National Licenses
  5. 5
    Hu, Y. ; Brown, J.W.S. ; Waugh, R. ; Turner, P.C.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0167-4781
    Keywords:
    Potato ; RNA processing ; Small nuclear RNA ; U6 snRNA ; snRNA gene
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0167-4781(91)90217-A

    Articles: DFG German National Licenses
  6. 6
    Waugh, R. ; Clark, G. ; Brown, J.W.S.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0378-1119
    Keywords:
    Pre-mRNA splicing ; flanking regions ; gene family ; polymerase chain reaction ; promoter ; pseudogene ; recombinant DNA ; regulatory element ; spliceosome ; uridylate-rich small nuclear ribonucleoprotein particle
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0378-1119(91)90319-7

    Articles: DFG German National Licenses
  7. 7
    Staff View
    ISSN:
    0378-1119
    Keywords:
    apoplastic enzyme ; reverse transcription-polymerase chain reaction ; single-stranded conformational polymorphism ; β-Fructosidase
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0378-1119(94)90007-8

    Articles: DFG German National Licenses
  8. 8
    Staff View
    ISSN:
    0014-5793
    Keywords:
    Ferredoxin ; Potato ; Solanum tuberosum ; Sucrose cleavage
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0014-5793(94)01105-2

    Articles: DFG German National Licenses
  9. 9
    Staff View
    ISSN:
    0031-9422
    Keywords:
    Solanaceae ; Solanum tuberosum ; invertase ; potato tuber ; processing.
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/S0031-9422(00)90564-8

    Articles: DFG German National Licenses
  10. 10
    Staff View
    ISSN:
    0031-9422
    Keywords:
    Solanaceae ; Solanum tuberosum ; invertase ; potato ; purification.
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0031-9422(92)80331-8

    Articles: DFG German National Licenses
  11. 11
    Staff View
    ISSN:
    0014-5793
    Keywords:
    Affinity-purified antibodies ; PRP8 ; Pre-mRNA splicing ; Spliceosomal protein ; Tobacco ; snRNP
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0014-5793(93)81315-Q

    Articles: DFG German National Licenses
  12. 12
    Waugh, R. ; Power, W.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0167-7799
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Process Engineering, Biotechnology, Nutrition Technology
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1016/0167-7799(92)90212-E

    Articles: DFG German National Licenses
  13. 13
    Staff View
    ISSN:
    1432-2277
    Keywords:
    Liver transplantation, hepatic failure ; Hepatic failure, liver transplantation
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Abstract This paper reports the clinical syndrome of fulminant hepatic failure (FHF) following liver transplantation. FHF was defined as the sudden onset of liver failure [encephalopathy and prolonged International Normalised Ratio (INR)] without arterial thrombosis in the setting of a liver allograft. FHf post-transplant was seen in 8/154 (5.2%) adult patients undergoing transplantation. These eight patients developed a clinical syndrome characterised by: (a) a rapid rise in ALT levels to above 1000 U/l (mean maximum 1600 U/l), (b) a sudden increase in the INR to above 5 (mean maximum 5.6), (c) the development of high fever, (d) the persistence of thrombocytopenia (mean nadir 40×109/dl), (e) a progressive rise in the bilirubin (mean maximum 400 μmol/l) and (f) the development of hepatic encephalopathy. In seven cases this syndrome occurred following good initial graft function at day 6 post (mean)-transplant. In one case the above syndrome developed immediately after liver transplantation. Four of the eight patients developed multiorgan failure associated with systemic acidosis (mean pH 6.84). All of these patients died (mean day 11). Four patients developed systemic alkalosis. Two of these four patients underwent successful retransplantation (on days 12 and 13) and remain alive at a mean of 11 months post-transplant. Six of the eight patients received OKT3 therapy without any apparent affect on clinical outcome. Compared to a control group of patients (n=28), 2/8 versus 2/28 had a positive crossmatch with donor lymphocytes (P=NS), 1/8 versus 7/28 were ABO-non-identical (P=NS), 3/8 versus 10/21 had total MHC mismatches (P=NS) and 5/7 versus 6/16 had UW ischemic times above 10 h (P=NS). No patients had main hepatic artery thrombosis on angiography although four patients had evidence of intrahepatic microthrombi or arterial necrosis at autopsy. In all cases the histology showed massive haemorrhagic necrosis. Three cases had evidence of veno-occlusive lesions whilst foam cell arteriopathy was seen in two cases. Immunofluorescence was performed in three cases. In two cases there was evidence of immunoglobulin, complement and fibrin deposition in blood vessels. In conclusion, we describe an uncommon clinical syndrome occurring post liver transplant. This syndrome represents humorally mediated allograft rejection but there seems to be no relationship with tissue matching (antibody, ABO, MHC) or donor ischaemic times. If recognised earlier in the absence of multiorgan failure, urgent retransplantation seems to be the only effective therapy.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00366706

    Articles: DFG German National Licenses
  14. 14
    Provan, J. ; Kumar, A. ; Shepherd, L. ; Powell, W. ; Waugh, R.
    Springer
    Published 1996
    Staff View
    ISSN:
    1432-203X
    Keywords:
    Key words: potato, simple sequence repeats, somatic hybrids
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract. We have utilised simple sequence repeat (SSR) polymorphism to analyse two sets of potential intra-specific hybrids of potato. Two primer pairs were used and both showed that one set of fusion products could not be true heterokaryons. In the other set, one of the primer pairs showed that unique bands in each of the parents were present in all of the hybrids, unambiguously demonstrating hybridity. This simple and robust, high-resolution assay can be used at the callus level and is amenable to automation, making it possible to reduce greatly the time required to screen a large number of potential somatic hybrids.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s002990050205

    Articles: DFG German National Licenses
  15. 15
    Staff View
    ISSN:
    1432-203X
    Keywords:
    Somatic hybrids ; Potato ; Reducing sugar ; RAPD
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Summary A somatic hybridisation programme was undertaken to evaluate the expression of reducing sugar accumulation in potato. Interspecific hybrids created between the Solanum tuberosum cultivar Record and the diploid species Solanum phureja were evaluated at the morphological and molecular levels. These analyses indicated that the protoplast regenerants were partial (asymmetric) hybrids which had undergone elimination of S. phureja chromosomes. Tubers of the parents exhibited significant differences for reducing sugar accumulation during cold storage with S. phureja having lower levels of glucose and fructose than Record. The somatic hybrids resembled the S. phureja parent in terms of reducing sugar accumulation demonstrating that low reducing sugar accumulation is dominant to high reducing sugar accumulation in these particular genotypes. These results are discussed in relation to the exploitation of asymmetric hybridisation for the production of potato genotypes for the potato processing industry.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00234147

    Articles: DFG German National Licenses
  16. 16
    Waugh, R. ; Baird, E. ; Powell, W.
    Springer
    Published 1992
    Staff View
    ISSN:
    1432-203X
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract Randomly Amplified Polymorphic DNAs were employed to demonstrate that potato dihaploids generated after interspecific pollination of a tetraploid Solanum tuberosum cultivar (Pentland Crown) by Solanum phureja dihaploid inducer clones could not be of parthenogenetic origin. Of six different 10-mer oligonucleotides, four generated products from total potato dihaploid genomic DNAs which were not derived from the S. tuberosum parent. Gel electrophoresis and Southern analysis indicated that these amplified bands originated from S. phureja. The results are discussed in the context of recent cytological and molecular evidence which demonstrates that potato dihaploids are aneusomatic (Clulow et al. 1991) and emphasises this approach as a general methodology for the detection of alien gene introgression in both natural and cultivated plant populations.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00232692

    Articles: DFG German National Licenses
  17. 17
    Staff View
    ISSN:
    1432-203X
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract The phenetic relationships between 18 Coffea accessions representing 11 of the most important Coffea species employed in current breeding programmes were examined using RAPD markers and chloroplast and mitochondrial genome specific sequence tagged sites (STS). Estimates of variability based on the number of shared RAPD amplification products placed the species into three distinct groups which were consistent with derived chloroplast DNA phenotypes, the geographical origins of the species and previous studies based on morphological characteristics and RFLPs. C. eugenioides (2n = 2x = 22) exhibited the greatest similarity to the cultivated C. arabica (2n = 4x = 44) and may represent its maternal progenitor. The results are discussed in the context of strategies for Coffea improvement.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00232367

    Articles: DFG German National Licenses
  18. 18
    Orozco-Castillo, C. ; Chalmers, K.J. ; Powell, W. ; Waugh, R.
    Springer
    Published 1996
    Staff View
    ISSN:
    1432-203X
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract The phenetic relationships between 18 Coffea accessions representing 11 of the most important Coffea species employed in current breeding programmes were examined using RAPD markers and chloroplast and mitochondrial genome specific sequence tagged sites (STS). Estimates of variability based on the number of shared RAPD amplification products placed the species into three distinct groups which were consistent with derived chloroplast DNA phenotypes, the geographical origins of the species and previous studies based on morphological characteristics and RFLPs. C. eugenioides (2n=2x=22) exhibited the greatest similarity to the cultivated C. arabica (2n=4x=44) and may represent its maternal progenitor. The results are discussed in the context of strategies for Coffea improvement.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s002990050028

    Articles: DFG German National Licenses
  19. 19
    Svetina, S. ; Žekš, B. ; Waugh, R. E. ; Raphael, R. M.
    Springer
    Published 1998
    Staff View
    ISSN:
    1432-1017
    Keywords:
    Key words Membrane microtubes ; Membrane elasticity ; Phospholipid translocation
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Physics
    Notes:
    Abstract Observations over extended times of a lipid microtube (tether) formed from a lecithin vesicle have shown that under constant external loads the tether exhibits a continuous slow growth. It is considered that this growth is a consequence of the net transbilayer movement of phospholipid molecules in a direction which relieves the membrane strain resulting from the elastic deformation of the vesicle. The elastic deformation mode responsible for this effect is identified as the relative expansion of the two membrane layers reflecting the non-local contribution to membrane bending. An equation for the consequent rate of transbilayer movement of phospholipid molecules is derived. The dynamic behavior of the system is modeled by including frictional contributions due to interlayer slip and Stokes drag on the glass bead used to form the tether. The general numerical solution reveals a complex dependence of the tether growth rate on the system parameters and a continuous increase in the rate of tether growth at long times. Closed form expressions approximating the system behavior are derived and the conditions under which they can be applied are specified. Modeling the mechanically-driven lipid transport as a simple, stochastic, thermal process, allows the rate of lipid translocation to be related to the equilibrium transbilayer exchange rate of phospholipid molecules. Consideration of experimental results shows that the time constant for mechanically-driven translocation is shorter than the time for diffusion-driven translocation by approximately two orders of magnitude, indicating that lipid translocation is not a simple diffusive process.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s002490050126

    Articles: DFG German National Licenses
  20. 20
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Key words Barley ; Microsatellites ; Linkage map ; Genetic similarity (GS) ; Polymorphism information content (PIC)
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract  By searching the EMBL DNA sequence database, we were able to develop 39 new, database-derived barley microsatellites. Eighteen of these EMBL microsatellites were mapped either to the interspecific barley map Lerche×BGRC41936 (L×41), the Igri×Franka map (I×F, Graner et al. 1991), or to both maps simultaneously. In addition, all 39 EMBL microsatellites were assigned to individual barley chromosomes by PCR screening of wheat barley addition lines. Both studies verified a random distribution of the microsatellites within the barley genome. Subsequently, 22 EMBL microsatellites were used to assess the genetic similarity among a set of 28, mainly German, barley cultivars and two wild form accessions. Spring and winter cultivars could be easily differentiated using the first coordinate of a principal coordinate analysis. Whereas the group of spring barley cultivars appeared rather homogeneous, winter barley cultivars could be divided into three subgroups. Two H. v. ssp. spontaneum accessions were included in the assessment of genetic similarity. They were placed among the winter barley cultivars. Based on the assessment of the 30 barley cultivars and accessions, the polymorphism information content (PIC) of each EMBL microsatellite has been calculated. The average PIC value among the EMBL microsatellites was equal to 0.38, which ascertains the value of these microsatellites as a genetic tool in barley genome research projects.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s001220051527

    Articles: DFG German National Licenses