Search Results - (Author, Cooperation:R. W. Tsien)
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1Latest Papers from Table of Contents or Articles in PressH. Park ; Y. Li ; R. W. Tsien
American Association for the Advancement of Science (AAAS)
Published 2012Staff ViewPublication Date: 2012-02-22Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Cells, Cultured ; *Exocytosis ; Imaging, Three-Dimensional ; *Membrane Fusion ; Microscopy, Fluorescence ; Neurons/physiology/ultrastructure ; Presynaptic Terminals/*physiology/ultrastructure ; Rats ; Synaptic Transmission ; Synaptic Vesicles/*physiology/ultrastructure ; Trypan BluePublished by: -
2Latest Papers from Table of Contents or Articles in PressB. Li ; M. R. Tadross ; R. W. Tsien
American Association for the Advancement of Science (AAAS)
Published 2016Staff ViewPublication Date: 2016-02-26Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Autistic Disorder/genetics/metabolism ; Calcium Channel Blockers/pharmacology ; Calcium Channels, L-Type/chemistry/*metabolism ; *Calcium Signaling ; Calcium-Calmodulin-Dependent Protein Kinase Type 2/*metabolism ; Cells, Cultured ; Cyclic AMP Response Element-Binding Protein/metabolism ; *Gene Expression Regulation ; HEK293 Cells ; Hippocampus/cytology ; Humans ; Long QT Syndrome/genetics/metabolism ; Neuronal Plasticity/*genetics ; Neurons/drug effects/*metabolism ; Nimodipine/pharmacology ; Protein Conformation/drug effects ; Rats ; Rats, Sprague-Dawley ; Receptors, N-Methyl-D-Aspartate/metabolism ; Synapses/metabolism ; Syndactyly/genetics/metabolismPublished by: -
3Latest Papers from Table of Contents or Articles in PressT. Karayannis ; E. Au ; J. C. Patel ; I. Kruglikov ; S. Markx ; R. Delorme ; D. Heron ; D. Salomon ; J. Glessner ; S. Restituito ; A. Gordon ; L. Rodriguez-Murillo ; N. C. Roy ; J. A. Gogos ; B. Rudy ; M. E. Rice ; M. Karayiorgou ; H. Hakonarson ; B. Keren ; G. Huguet ; T. Bourgeron ; C. Hoeffer ; R. W. Tsien ; E. Peles ; G. Fishell
Nature Publishing Group (NPG)
Published 2014Staff ViewPublication Date: 2014-05-30Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Antipsychotic Agents/pharmacology ; Behavior, Animal/drug effects/physiology ; Dopamine/*metabolism ; Electrical Synapses/genetics/ultrastructure ; Female ; Genotype ; Humans ; Male ; Membrane Proteins/*genetics/*metabolism ; Mice ; Nerve Tissue Proteins/*genetics/*metabolism ; Polymorphism, Single Nucleotide ; *Signal Transduction ; Synaptic Transmission/*genetics ; gamma-Aminobutyric Acid/*metabolismPublished by: -
4Latest Papers from Table of Contents or Articles in PressA. S. Yoo ; A. X. Sun ; L. Li ; A. Shcheglovitov ; T. Portmann ; Y. Li ; C. Lee-Messer ; R. E. Dolmetsch ; R. W. Tsien ; G. R. Crabtree
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-07-15Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Adult ; Basic Helix-Loop-Helix Transcription Factors/genetics/metabolism ; Biomarkers/analysis/metabolism ; Cell Differentiation/*genetics ; Cell Line ; Cell Lineage/genetics ; DNA-Binding Proteins/genetics/metabolism ; Excitatory Postsynaptic Potentials/physiology ; Fibroblasts/*cytology/*metabolism ; Humans ; Infant, Newborn ; MicroRNAs/*genetics/metabolism ; Microtubule-Associated Proteins/analysis/metabolism ; Nerve Tissue Proteins/genetics/metabolism ; Neurons/*cytology/*metabolism ; Neuropeptides/genetics/metabolism ; Transcription Factors/genetics/metabolism ; Tubulin/analysis/metabolismPublished by: -
5Latest Papers from Table of Contents or Articles in PressS. F. Owen ; S. N. Tuncdemir ; P. L. Bader ; N. N. Tirko ; G. Fishell ; R. W. Tsien
Nature Publishing Group (NPG)
Published 2013Staff ViewPublication Date: 2013-08-06Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Action Potentials/*drug effects ; Animals ; Brain/metabolism ; Cholecystokinin/metabolism ; Excitatory Postsynaptic Potentials/drug effects/physiology ; Feedback, Physiological/drug effects ; Glycine/pharmacology ; Hippocampus/*cytology/physiology ; Interneurons/*drug effects/metabolism ; Mice ; Neural Pathways/drug effects ; Oxytocin/*pharmacology ; Pyramidal Cells/drug effects/metabolism ; Rats ; Receptors, Oxytocin/agonists/metabolism ; Rhodopsin/metabolism ; Synapses/drug effects/metabolism ; Synaptic Transmission/*drug effects ; Threonine/pharmacologyPublished by: -
6Articles: DFG German National LicensesReuter, H. ; Stevens, C. F. ; Tsien, R. W. ; Yellen, G.
[s.l.] : Nature Publishing Group
Published 1982Staff ViewISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Tissue cultured myocytes were obtained from neonatal rat hearts5*6. After seeding, the cells were incubated for 2-4 days in a culture medium containing 0.1 mM 8-bromo cyclic AMP to increase the likelihood of detecting single Ca channels7. Most cells used for our experiments had spindle-like ...Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Fig. 1 Sodium currents in the rabbit Purkinje fibre. Membrane currents elicited by depolarisations from a holding potential of -77 mV. Traces are the average of two sweeps (10 kHz filtering) and have been corrected for steady current by computer subtrac-tion. (The size of the steady current is ...Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Short Purkinje fibre preparations from calf hearts were voltage-clamped by a conventional two-microelectrode method. In some experiments, contractile force was measured with a piezoelectric force transducer (Endevco 87102). Interference from the excitatory sodium current was prevented by blocking ...Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] We studied the effects of strophanthidin on the electrical and mechanical activity of cardiac Purkinje fibres from calf hearts. The aglycone strophanthidin was preferred to cardiac glycosides such as ouabain because its effects are more readily reversible. Purkinje fibres were used because recent ...Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Vesicle fusion and recycling are particularly critical for ongoing neurotransmitter release in the small nerve terminals of the brain, which typically contain about 30 functional vesicles. However, the modes of exocytosis and endocytosis that operate at synapses of the central nervous system ...Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 1749-6632Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Natural Sciences in GeneralType of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStrong, J. A. ; Fox, A. P. ; Tsien, R. W. ; Kaczmarek, L. K.
[s.l.] : Nature Publishing Group
Published 1987Staff ViewISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] The function of the neurosecretory bag cell neurons is to initiate a sequence of reproductive behaviours. Activation of these cells leads to major changes in their electrical properties, controlled in part by activation of protein kinase C7. This calcium-, phospholipid-, diacylglycerol-dependent ...Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Organic inhibitors of calcium influx prevent outward as well as inward current through cardiac calcium channels but do not slow current activation. Although block is antagonized by raising external calcium or barium concentrations, the competitive effect of permeant cations does not occur at the ...Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Current recordings were made from freshly dispersed smooth muscle cells from rabbit ear artery14 with patch-clamp techniques.15. Figure la shows a whole-cell recording from a cell held at -50 mV in a standard Tyrode solution. Bath application of 1 jjiM ATP evoked an inward current of several ...Type of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesNilius, B. ; Hess, P. ; Lansman, J. B. ; Tsien, R. W.
[s.l.] : Nature Publishing Group
Published 1985Staff ViewISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Figure 1 illustrates some of the features that distinguish the two types of Ca channel. Activity of the previously described type of cardiac Ca channel (Fig. la) was evoked by depolarizing voltage-clamp pulses to +10mV from a holding potential of -50 mV; with HOmM Ba in the pipette, channel ...Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 1420-9071Keywords: Heart cells ; calcium channels ; single channelsSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Summary Properties of cardiac Ca channels have come into sharper focus with the advent of single cell preparations and suction pipette recording methods. We briefly summarize our present picture of the gating and permeation properties of the conventional, dihydrophyridine-sensitive type of Ca channel (L-type). Distincitive features of a second type of voltage-gated Ca channel (T-type) are discussed.Type of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 1432-1424Source: Springer Online Journal Archives 1860-2000Topics: BiologyChemistry and PharmacologyNotes: Summary The kinetics of ionic current mechanisms in excitable membranes are analyzed. It is assumed that there are voltage-dependent reactions occurring in the membrane which are independent of the flow of ionic current. The experimental evidence for this assumption is reviewed in the light of more recent results on the kinetics of conductance changes in cardiac membranes. Rate equations are then obtained using transition state theory and assuming that each reaction is rate limited by only one energy barrier. These equations give simple exponential functions for the voltage dependence of the rates. More complex functions may be obtained by assuming that more than one energy barrier is rate limiting. The single-barrier equations are used to estimate the energies of formation of the transition state. In most cases, the entropy of formation is positive but there is no systematic order in the estimated enthalpies. These results are contrasted with those for the ion permeation process itself which normally has a negative entropy of activation. This contrast reinforces the assumption that the reactions controlling membrane permeability are distinct from the ion permeation process itself. The significance of the positive entropy of formation of the transition state in the permeability reactions is discussed, and it is suggested that the membrane structures underlying these reactions may change their degree of hydration during the formation of the transition state.Type of Medium: Electronic ResourceURL: