Search Results - (Author, Cooperation:R. Richman)

2013-05-31

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Amino Acid Sequence ; Animals ; Animals, Genetically Modified ; Ataxin-1 ; Ataxins ; Cell Line, Tumor ; Disease Models, Animal ; Down-Regulation/drug effects ; Drosophila melanogaster/genetics/*metabolism ; Female ; Humans ; MAP Kinase Signaling System/drug effects ; Male ; Mice ; Mitogen-Activated Protein Kinases/*metabolism ; Molecular Sequence Data ; Molecular Targeted Therapy ; Nerve Tissue Proteins/chemistry/genetics/*metabolism/*toxicity ; Nuclear Proteins/chemistry/genetics/*metabolism/*toxicity ; Phosphorylation ; Protein Stability/drug effects ; Ribosomal Protein S6 Kinases, 90-kDa/deficiency/genetics/*metabolism ; Spinocerebellar Ataxias/*metabolism/*pathology ; Transgenes ; ras Proteins/*metabolism

1089-7623

AIP Digital Archive

Physics

Electrical Engineering, Measurement and Control Technology

An apparatus that is capable of producing repeated impacts of erodent particles on a solid surface, in vacuum or controlled atmospheres, is described. It was developed specifically to correct the deficiencies of previous designs. It consists of an electromagnet, a laminated iron core, and an aluminum launcher (sabot), all contained in a sturdy frame that fits easily into a bell-jar vacuum system. Particle velocities can be readily varied over the range 3–100 m/s, with incidence angles from 10° to 90°. Such a device should find application in fundamental studies of solid-particle erosion. © 1995 American Institute of Physics.

Electronic Resource

0004-8402

Philosophy

0004-8402

Philosophy

0012-1606

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

0045-6039

differentiation ; initiation sites ; replication transcription

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

0045-6039

DNA replication ; base composition ; early and late S phase

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Electronic Resource

1432-0428

Insulin ; fetal rat hepatocytes ; glycogen ; endocytosis ; degradation ; retroendocytosis

Springer Online Journal Archives 1860-2000

Medicine

Summary We studied insulin processing and hepatic glycogenesis in cultured hepatocytes isolated from rat fetuses of 17, 19, and 21 days of gestation. Steady-state insulin binding increased by 250% between days 17 and 19, from 145±8 to 361±52 fmol/mg protein, and by an additional 40% (405±69 fmol/mg protein) by 21 days of gestation. At 37°C, 125I-insulin was rapidly (t1/2〈5 min) internalized by hepatocytes at all three ages, reaching maximal levels (63–76% of the total cell-associated radioactivity) by 15 min. 125I-labelled degradation products appeared rapidly (t1/2〈15 min) within the cells. Yet, the majority (68–77%) of the intracellular radioactivity consisted of intact 125I-insulin, even after 4 h at 37°C. Hepatocytes pre-loaded with 125I-insulin and then acid-stripped of surface-bound radioactivity, rapidly released both intact 125I-insulin (retroendocytosis) and its radiolabelled degradation products. While intact insulin was initially released more rapidly (t1/2〈6 min), and reached a plateau after 15–30 min, the degradation products continued to accumulate in the medium for at least 4 h. Methylamine inhibited intracellular 125I-insulin degradation at all three gestational ages and also blocked insulin-stimulated glycogenesis in 19- and 21-day hepatocytes, without altering basal glycogen synthesis. Insulin-stimulated glycogenesis was not induced in 17-day fetal rat hepatocytes in control or methylamine-treated cultures. We conclude that both degradative and retroendocytotic pathways for processing insulin are present in fetal rat hepatocytes by 17 days of gestation. Further, insulin-receptor processing was functionally related to the glycogenic action of insulin in responsive 19- and 21-day fetal rat hepatocytes

Electronic Resource

1573-4803

Springer Online Journal Archives 1860-2000

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Abstract Geometric surface relief effects have been observed in association with the γ→α allotropic transformation in iron. The selected-area electron channelling pattern technique has been used to establish that tent shaped geometric relief effects, common to single α precipitate plates in Fe-C and Fe-C-X alloys, are also associated with similar single crystal morphologies in pure iron. These experimental observations extend to the allotropic transformation in iron the same rationale for the relationship of interfacial structure to surface relief effects that is known to apply in the instance of precipitation reactions. In addition it provides support for an earlier proposal that “massive” transformations can be subject to the same crystallographic constraints as precipitation processes.

Electronic Resource

0021-9541

Life and Medical Sciences ; Cell & Developmental Biology

Wiley InterScience Backfile Collection 1832-2000

Biology

Medicine

Addition of H1 histone or polylysine (10 μg/ml) to cultured Friend erythroleukemia cells or to two mouse lymphoma cell lines (el-4 and S-49) increased levels of cell division in these cultures. There is a stimulation of incorporation of labeled thymidine into DNA in cultures containing H1 histone and polylysine. DNA fiber autoradiographic experiments revealed that replicon size is decreased in the cells cultured with H1 histone and polylysine at later periods of culture.

7 Ill.

Electronic Resource