Search Results - (Author, Cooperation:R. Pickering)

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    Staff View
    Publication Date:
    2011-07-30
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Carcinoma/drug therapy/*genetics/virology ; Carcinoma, Squamous Cell ; Cell Cycle Proteins/*genetics ; Codon, Nonsense ; Exons ; F-Box Proteins/*genetics ; Gene Dosage ; *Genes, Tumor Suppressor ; Genes, p53 ; Head and Neck Neoplasms/drug therapy/*genetics/virology ; Humans ; INDEL Mutation ; *Mutation ; Mutation, Missense ; Neoplasms, Squamous Cell/drug therapy/*genetics/virology ; Oligonucleotide Array Sequence Analysis ; Oncogenes ; Papillomaviridae/isolation & purification ; Papillomavirus Infections/virology ; Receptor, Notch1/chemistry/*genetics ; Sequence Analysis, DNA ; Smoking ; Tobacco ; Ubiquitin-Protein Ligases/*genetics
    Published by:
    Latest Papers from Table of Contents or Articles in Press
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    R. Pickering ; P. H. Dirks ; Z. Jinnah ; D. J. de Ruiter ; S. E. Churchil ; A. I. Herries ; J. D. Woodhead ; J. C. Hellstrom ; L. R. Berger
    American Association for the Advancement of Science (AAAS)
    Published 2011
    Staff View
    Publication Date:
    2011-09-10
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; *Fossils ; *Geologic Sediments ; Geology/methods ; *Hominidae/anatomy & histology/classification ; Magnetics ; Radiometric Dating ; South Africa ; Time ; Uranium
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  5. 5
    Staff View
    Publication Date:
    2018-02-23
    Publisher:
    BMJ Publishing
    Electronic ISSN:
    2044-6055
    Topics:
    Medicine
    Keywords:
    Open access, Health services research, Nursing
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  6. 6
    Toubia-Rahme, H. ; Johnston, P. A. ; Pickering, R. A. ; Steffenson, B. J. ; Graner, A.

    Oxford, UK : Blackwell Publishing Ltd
    Published 2003
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Septoria speckled leaf blotch (SSLB), caused by Septoria passerinii, has become one of the most serious diseases of barley in the Upper Midwest region of the USA. The recombinant line 36L5 derived from a backcross of the susceptible barley cultivar ‘Emir’ and a resistant Hordeum bulbosum parent Cb2920/4/Colch was found to be resistant to S. passerinii. Two doubled haploids derived from 36L5 were backcrossed to cv.‘Emir’ to obtain two BCF2 populations for determining the inheritance of resistance to S. passerinii. BCF2 progeny and BCF2:3 families were evaluated at the seedling stage in the greenhouse for reaction to S. passerinii. BCF2 progeny and BCF2:3 families from both crosses segregated 3 : 1 (resistant : susceptible), and 1:2:1 (resistant : segregating : susceptible), respectively, indicating that the H. bulbosum-derived SSLB resistance is conferred by a single dominant gene. The H. bulbosum introgressions were positioned on chromosome 4HL by genomic and fluorescent in situ hybridizations (GISH and FISH, respectively) and by Southern hybridization with the rye repetitive sequence pSc119.2. These findings indicate that SSLB resistance in H. bulbosum has the potential to be transferred and utilized in barley breeding programs.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  7. 7
    Pickering, R. A.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1989
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    A hybrid plant was regenerated from embryo-derived callus after crossing Hordeum vulgare with Psathyrostachys fragilis. A mciotic analysis of pollen mother cells revealed the frequent occurrence of one ring bivalent and twelve univalents. It appeared that eight H. vulgare and six P. fragilis chromosomes were present and an unknown duplicate H. vulgare chromosome had compensated for the loss of one P. fragilis chromosome. Although it remains unclear whether the anomaly was present in the original explain or had occurred during the callus phase, the technique could be used to investigate genome homology between the two species.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  8. 8
    Pickering, R. A.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1991
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    A trisomic series was produced from a triploid plant of barley (Hordeum vulgare L. cv. ‘Golden Promise’) derived from tissue culture. Its characteristics are briefly described and compared with two trisomic series reported previously. Trisomic number 1 performed poorly under glasshouse conditions. Number 2 failed to set any seed after selfing and must be maintained by pollinating with ‘Golden Promise’. The series was subsequently used to locate a recessive chlorina gene on barley chromosome 3.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  9. 9
    Pickering, R. ; Klatte, S. ; Butler, R. C.

    Oxford, UK : Blackwell Publishing Ltd
    Published 2005
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Rod or ring bivalents at metaphase I (MI) reflect whether crossing-over has occurred on one or both pairs of chromosome arms, respectively, during meiotic prophase (pachytene). In cultivated barley (Hordeum vulgare) the highest frequencies of rod bivalents were found for satellite chromosomes (either 5H or 6H). To distinguish between chromosome 5H and 6H associations, a recombinant line derived from an H. vulgare × H. bulbosum hybrid with a hemizygous introgression from H. bulbosum on the short arm of chromosome 6H was probed with 45S rDNA and pSc119.2 using fluorescent in situ hybridization and compared with non-recombinant H. vulgare. The frequencies of rod bivalents, caused by failure of the short arms of 5H chromosomes (5HS) to associate during meiosis in the recombinant line, were similar to the frequencies of rod bivalents involving the satellite chromosomes in H. vulgare. Hence, rod bivalent formation in H. vulgare satellite chromosomes is probably based on reduced association of 5HS, corresponding to a low frequency of genetic recombination. 5HS is, therefore, more likely to show linkage disequilibrium than the other chromosome arms.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  10. 10
    PICKERING, R. A. ; STEEFFENSON, B. J. ; HILL, A. M. ; BOROVKOVA, I.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1998
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    While studying powdery mildew resistance in a recombinant line (code 81882) derived from a Hordeum vulgare (cv. ‘Vada’) ×Hordeum bulbosum hybrid, a low infection type of resistance to leaf rust was observed. To determine the mode of inheritance of the leaf rust resistance and whether there was linkage between the two resistances, F2 and F3 progenies from crosses between 81882 and ‘Vada’ were inoculated with the leaf rust and powdery mildew pathogens. Southern blots were prepared using restricted DNA extracted from leaves of 82 F2 plants and four chromosome 2HS sequences were hybridized with the blots to define the length of the introgression. The leaf rust resistance appears to be inherited as a single dominant gene on chromosome 2HS, which co-segregates with the powdery mildew resistance. There was an almost complete association between the resistances and the respective molecular markers, but it is likely that the strong linkage results from the frequent inheritance of the introgressed H. bulbosum DNA as an intact segment of chromatin with only low levels of recombination within the segment.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  11. 11
    Gilpin, M. J. ; Pickering, R. A. ; Fautrier, A. G. ; Mcneil, D. L. ; Szigat, G. ; Hill, A. M. ; Kynast, R. G.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1997
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Anther culture (AC) was carried out on a fertile triploid hybrid between Hordeum vulgare L. (cultivated barley) and H. bulbosum L, (bulbous barley grass) to determine whether AC-derived regenerants differed from progeny obtained through selfing and backcrossing. Chromosome counts were carried out on all plants and DNA was extracted from them to prepare Southern blots for molecular analysis. To identify true recombinants, the blots were probed with rye repetitive sequence probes (pSc119.1 and pScl19.2). which hybridize strongly and specifically to H. bulbosum DNA. Twenty probes that detect single- or low-copy sequences were hybridized with Southern blots containing restricted DNA extracted from 25 AC-derived plants, 11 selfed and six backcrossed progeny that showed hybridizations with pScll9. Although restriction fragment length polymorphisms (RFLPs) were only observed using probes that map to four of the possible 14 chromosome arms, an introgression associated with chromosome 6HS was frequently observed among plants derived from AC. selfing and backcrossing. Plants from AC differed from selfed and backcrossed progeny in their chromosome number; unique RFLP bands that were occasionally observed may indicate chromosomal rearrangements.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  12. 12
    Pickering, R. A.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1987
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Investigations were carried out to assess the suitability of the intergeneric cross Hordeum vulgare×Psathyrostachys fragilis for haploid barley production. H. vulgare cvs. ‘Emir’ and ‘Vada’ were each pollinated with P. fragilis P.I. 343192 and plants regenerated from embryos cultured on a modified B5 medium. Seed sets on ‘Vada’ were significantly lower than on ‘Emir’, and all the planes from ‘Vada’×P. fragile remained hybrid. Several of these flowered but there was little pairing between the parental chromosomes. Most of the plants from ‘Emir’×P. fragilis died, as seedlings but 3 plants developed into haploid barley. Because of the practical limitations of pollen availability from P. fragilis and the inconsistencies in haploid plant formation, it is unlikely that the cross will prove as valuable as that between H. vulgare×H. bulbosum for a doubled haploid barley programme.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  13. 13
    Pickering, R. A. ; Fautrier, A. G.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1993
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Hordeum bulbosum has several desirable attributes, including disease resistance, which would be worthwhile transferring to H. vulgare. Despite homoeologous chromosome pairing in the interspecific hybrids, there have been few reports of successful gene introgression between the two species. A possible explanation for this is that recombinant male gametes are at a competitive disadvantage with normal balanced gametes during post-pollination events. To circumvent this problem, the possibility of obtaining plants directly from immature pollen grains was investigated. Anthers from diploid, triploid and tetraploid H. vulgare × H. bulbosum hybrids were cultured on defined media. Only hybrids with dehiscent anthers in vivo responded in culture, and after transfer of calli and embryoids to regeneration medium, 36 albino and 12 green plants were obtained. Seven of the green regenerants survived, one of which contained 15 H. vulgare chromosomes (including one acrocentric chromosome) and one H. bulbosum chromosome. Another regenerant (Ac166) resembled a diploid H. vulgare × H. bulbosum hybrid but had partial anther dehiscence and a slightly modified chromosome constitution. Mostly normal H. vulgare progeny were obtained from crosses between H. vulgare cv.‘Emir’ and Ac166, but three plants involved chromosome additions and substitutions.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  14. 14
    Pickering, R. A. ; Wallace, A. R.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1994
    Staff View
    ISSN:
    1439-0523
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition
    Notes:
    Doubled haploid production from crosses between barley (Hordeum vulgare L.) and H. bulbosum L. is a well established technique for inducing homozygosity. One problem which occurs using certain barley cultivars is premature seed degeneration. In this paper, a post-pollination spray of gibberellic acid and 2,4-dichlorophenoxyacetic acid that delays the onset of senescence is described. The technique improved the number of high quality seeds to more than 75% of the seed set. This improvement should result in increased survival of embryos for culture and subsequent plant regeneration.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  15. 15
    THOMPSON, C. ; WESTWELL, P. ; VINEY, D. ; WILSON, B. A. ; HILL, F. ; HARRINGTON, R. ; BRYANT, T. N. ; PICKERING, R.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1996
    Staff View
    ISSN:
    1365-2516
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Sixty-four males with haemophilia were assessed with a series of neuropsychological tests and a structured interview for psychiatric symptoms. Thirty-one had been infected with human immunodeficiency virus (HIV) before the age of 18 and were in various stages of the disease at the time of testing and 33 were HIV negative. Sixteen male control subjects were recruited to match in age with the haemophilia group. The HIV-seropositive group were older than the HIV-negative group due to the cohort effect of the time of infection. Contrary to predictions from the known effects of HIV on the central nervous system the HIV-seropositive group performed better on many of the tests than the HIV-seronegative group. In some but not all of the tests this may have been an age effect. There was very little psychiatric morbidity, consistent with the view that high levels of psychological support provided by the haemophilia units can alleviate the effects of the illness on emotions and behaviour. These findings form a baseline for a 2-year follow-up study which is in progress.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  16. 16
    Pickering, R.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0307-4412
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
  17. 17
    Pickering, R.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0307-4412
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Biology
    Chemistry and Pharmacology
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses
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