Search Results - (Author, Cooperation:R. M. Evans)

2015-03-06

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

2014-07-22

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Blood Glucose/metabolism ; Body Weight/drug effects ; Diabetes Mellitus, Experimental/drug therapy/metabolism ; Diabetes Mellitus, Type 2/metabolism ; Diet, High-Fat ; Dose-Response Relationship, Drug ; Fibroblast Growth Factor 1/administration & dosage/adverse effects/*pharmacology ; Glucose/*metabolism ; Glucose Tolerance Test ; Humans ; Insulin/*metabolism ; Insulin Resistance ; Liver/drug effects/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Obese ; Mitogens/pharmacology ; Muscle, Skeletal/drug effects/metabolism ; Receptor, Fibroblast Growth Factor, Type 1/metabolism

2015-11-19

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Adipose Tissue/*cytology/*immunology ; Aging/*immunology ; Animals ; Diabetes Mellitus, Type 2/metabolism ; Glucose/metabolism ; Inflammation/immunology/metabolism ; Insulin Resistance/*immunology ; Macrophages/immunology ; Male ; Metabolic Syndrome X/immunology/metabolism ; Mice ; Obesity/metabolism ; T-Lymphocytes, Regulatory/*cytology/*immunology

2012-04-24

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Adipocytes/drug effects/metabolism/pathology ; Animals ; Base Sequence ; Cell Size/drug effects ; Diabetes Mellitus, Experimental/chemically induced/genetics/pathology ; Diet, High-Fat/adverse effects ; Fibroblast Growth Factor 1/deficiency/*genetics/*metabolism ; *Homeostasis/drug effects ; Humans ; Inflammation/genetics ; Insulin/metabolism ; Insulin Resistance ; Intra-Abdominal Fat/drug effects/*metabolism/pathology ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Necrosis/enzymology ; PPAR gamma/*metabolism ; Promoter Regions, Genetic/genetics ; Response Elements/genetics

2013-08-30

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Bacteria/*isolation & purification ; Biomarkers/*metabolism ; *Diet ; Female ; Gastrointestinal Tract/*microbiology ; Humans ; Male ; Metagenome/*genetics

2013-06-04

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Alleles ; Animals ; Base Sequence ; Binding Sites ; Down-Regulation/*genetics ; Enhancer Elements, Genetic/*genetics ; Gene Knockdown Techniques ; Macrophages/*metabolism ; Mice ; Nuclear Receptor Subfamily 1, Group D, Member 1/deficiency/genetics/*metabolism ; Organ Specificity ; Promoter Regions, Genetic/genetics ; RNA, Messenger/genetics/metabolism ; Response Elements/genetics ; Transcription, Genetic/*genetics

2015-10-31

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Animals ; Apoptosis Regulatory Proteins/metabolism ; Biosynthetic Pathways ; Burkholderia ; Burkholderia Infections/complications ; Calcium-Binding Proteins/metabolism ; Escherichia coli/*immunology ; Inflammasomes/*immunology ; Insulin-Like Growth Factor I/*metabolism ; Intestines/*microbiology ; Mice ; Mice, Inbred C57BL ; *Microbiota ; Muscle, Skeletal/*metabolism ; Phosphatidylinositol 3-Kinase/metabolism ; Proto-Oncogene Proteins c-akt/metabolism ; Salmonella Infections/complications ; Salmonella typhimurium ; Wasting Syndrome/etiology/*immunology/*microbiology

2011-02-04

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Cell Differentiation/genetics ; Cell Line ; Cellular Reprogramming/*genetics ; CpG Islands/genetics ; DNA Methylation/*genetics ; Embryonic Stem Cells/cytology/metabolism ; Epigenomics ; Epistasis, Genetic/*genetics ; Fibroblasts/cytology/metabolism ; Genome, Human/*genetics ; Histones/metabolism ; Humans ; Induced Pluripotent Stem Cells/cytology/*metabolism ; Trophoblasts/cytology/metabolism

2012-03-31

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Biological Clocks/drug effects/genetics ; Circadian Rhythm/genetics/*physiology ; Cryptochromes/deficiency/genetics/metabolism ; *Energy Metabolism/genetics ; Feedback, Physiological ; Gene Expression Regulation ; Gene Regulatory Networks/genetics ; Homeostasis/genetics ; *Lipid Metabolism/genetics ; Liver/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Molecular Sequence Data ; Motor Activity/genetics/physiology ; Nuclear Receptor Subfamily 1, Group D, Member 1/deficiency/genetics/*metabolism ; Period Circadian Proteins/deficiency/genetics/metabolism ; Receptors, Cytoplasmic and Nuclear/deficiency/genetics/*metabolism ; Repressor Proteins/deficiency/genetics/*metabolism ; Transcriptome/genetics

2011-12-16

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; *Circadian Rhythm ; Corticosterone/blood ; Cryptochromes/genetics/*metabolism ; Dexamethasone/pharmacology ; Female ; *Gene Expression Regulation/drug effects ; Glucocorticoids/pharmacology ; Glucose Intolerance/genetics ; HEK293 Cells ; Humans ; Liver/enzymology/metabolism ; Mice ; Phosphoenolpyruvate Carboxykinase (GTP)/blood/metabolism ; Receptors, Glucocorticoid/*metabolism

2018-06-19

American Physical Society (APS)

0031-9007

1079-7114

Physics

Polymer, Soft Matter, Biological, Climate, and Interdisciplinary Physics

2018-08-04

American Physical Society (APS)

0031-9007

1079-7114

Physics

Polymer, Soft Matter, Biological, Climate, and Interdisciplinary Physics

2018-04-19

Royal Society

2054-5703

Natural Sciences in General

behaviour, palaeontology, evolution

2018-12-19

American Physical Society (APS)

1539-3755

1550-2376

Physics

Statistical Physics

2018-04-17

The American Society for Microbiology (ASM)

0270-7306

1098-5549

Biology

Medicine

1089-7623

AIP Digital Archive

Physics

Electrical Engineering, Measurement and Control Technology

A method for servolocking the beat frequency between two lasers is described in which the lasers in effect replace the voltage-controlled oscillator in an indirect frequency synthesizer. The system is simple, relatively free from systematic frequency errors, and has good stability as characterized by Allan variance measurements.

Electronic Resource

1089-7690

AIP Digital Archive

Physics

Chemistry and Pharmacology

The conditions of multiphase equilibrium are solved for generic polydisperse systems. The case of multiple polydispersity is treated, where several properties (e.g., size, charge, shape) simultaneously vary from one particle to another. By developing a perturbative expansion in the width of the distribution of constituent species, it is possible to calculate the effects of polydispersity alone, avoiding difficulties associated with the underlying many-body problem. Explicit formulas are derived in detail, for the partitioning of species at coexistence and for the shift of phase boundaries due to polydispersity. Convective fractionation is quantified, whereby one property (e.g., charge) is partitioned between phases due to a driving force on another. To demonstrate the ease of use and versatility of the formulas, they are applied to models of a chemically polydisperse polymer blend, and of fluid–fluid coexistence in polydisperse colloid–polymer mixtures. In each case, the regime of coexistence is shown to be enlarged by polydispersity. © 2001 American Institute of Physics.

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] For the plasmids used in the present experiments, the structural part of the growth hormone (GH) gene was fused to the promoter region of the mouse metallothionein-I (MT-I) gene (Fig. la; refs 12, 13), and it was anticipated that the fusion gene would be expressed in tissues in which endogenous ...

Electronic Resource

1432-0762

Key words Parent-offspring conflict ; Incubation temperature ; Herring gulls

Springer Online Journal Archives 1860-2000

Biology

Abstract According to Trivers (1974), parent-offspring (P-O) conflict arises because offspring are selected to solicit more care than parents are selected to provide. However, should benefits fail to increase with increasing care, the offspring optimum can be reduced to the point where predicted P-O conflict vanishes. We examined offspring demand and parental care in such a benefit-limited system in herring gulls (Larus argentatus). In this species, parents typically neglect their last-hatched (C-) egg during the final hours of hatching (pipped-egg stage), allowing mean temperature to drop by about 4°C, to near 33°C. Other studies indicate that no increased offspring benefit arises from increasing pipped egg incubation temperature above that level, but embryo damage occurs if temperature drops lower. In such a system, P-O conflict over preferred incubation temperature is predicted to be minimal or absent. We assessed phenotypic manifestations of conflict by determining incubation temperature preferences of parent and offspring independently. Temperature provided solely by parental initiative was 33.9°C (artificial eggs, corrected for embryonic heat production). Preferred incubation temperature of pipped embryos was measured by exposing them to moderate chilling (20°C) punctuated by 4-min periods of rewarming when they called. Temperature of vocally thermoregulating embryos stabilized around a mean of 32.9–33.4°C, about 0.5–1.0°C below parental preference. Acting independently, parents and embryos each maintained egg temperature at or near minimum developmentally safe levels. Results provided no evidence for phenotypic conflict, as predicted by a benefit-limited version of Trivers’ P-O conflict model. Benefit limitation may also be relevant to P-O conflict in other contexts such as feeding of newly-hatched young.

Electronic Resource

0091-7419

mouse L-929 cells ; “inside-out” configuration ; gel electrophoresis ; lectin-binding proteins ; Life Sciences ; Molecular Cell Biology

Wiley InterScience Backfile Collection 1832-2000

Biology

Chemistry and Pharmacology

Medicine

The topography and properties of plasma membrane proteins from mouse L-929 cells are studied by comparing their availability for enzymatic labeling on the external and internal surfaces of the membrane. In order to study the internal surface, phagolysosomes are prepared from cells after they ingest latex particles. The plasma membrane surrounding these seems to have an “inside-out” orientation. The sugars of the membrane glycoproteins in intact phagolysosomes are not available for interaction with lectins or available for periodate-borotritide labeling. A comparison of the lectin-binding proteins lableled by lactoperoxidase-catalyzed iodination on the external cell surface with those labeled on the internal cell surface suggests that a variety of plasma membrane glycoproteins span the lipid bilayer.Using two-dimensional gel electrophoresis it has been shown that selected proteins are labeled at both the internal and external faces of the plasma membrane. Analysis of the 2-D gel electrophoregrams reveals that there are two distinct prominent proteins at 60,000 and 100,000 daltons which are enzymatically iodinated from both sides of the membrane. The partial hydrolysis of the 100,000 dalton protein reveals that different peptides are iodinated when the iodination is performed on intact cells or on the phagolysosomes. These proteins are extensively phosphorylated in cells incubated with inorganic 32P. We conclude that the phagolysosome is probably oriented in an “inside-out” configuration and that this membrane preparation can be used to study the topographic organization of membrane proteins.The use of oriented membranes, selective labeling of proteins, and affinity separation of proteins in combination with gel electrophoresis to define the position and properties of proteins is discussed.

10 Ill.

Electronic Resource