Search Results - (Author, Cooperation:R. Jackson)

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  1. 1
    Staff View
    Publication Date:
    2018-03-06
    Publisher:
    Wiley-Blackwell
    Print ISSN:
    0017-467X
    Electronic ISSN:
    1745-6584
    Topics:
    Energy, Environment Protection, Nuclear Power Engineering
    Geosciences
    Published by:
    http://www.wiley.com/wiley-blackwell

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    Publication Date:
    2015-11-19
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Staff View
    Publication Date:
    2013-12-24
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Acyl Carrier Protein/*chemistry/*metabolism ; Binding Sites ; Catalytic Domain ; Cross-Linking Reagents/chemistry ; Crystallography, X-Ray ; Escherichia coli/*chemistry ; Fatty Acid Synthase, Type II/chemistry/metabolism ; Fatty Acids/*biosynthesis ; Histidine/metabolism ; Hydro-Lyases/chemistry/metabolism ; Magnetic Resonance Spectroscopy ; Models, Molecular ; Molecular Dynamics Simulation ; Protein Binding ; Protein Interaction Maps
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  4. 4
    Staff View
    Publication Date:
    2015-05-08
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  5. 5
    Staff View
    Publication Date:
    2018-05-23
    Publisher:
    The American Society for Microbiology (ASM)
    Print ISSN:
    0019-9567
    Electronic ISSN:
    1098-5522
    Topics:
    Medicine
    Published by:
    http://www.asm.org/

    Latest Papers from Table of Contents or Articles in Press
  6. 6
    R R D J N Subagyono, M Marshall, W R Jackson and A L Chaffee
    Institute of Physics (IOP)
    Published 2018
    Staff View
    Publication Date:
    2018-05-09
    Publisher:
    Institute of Physics (IOP)
    Print ISSN:
    1755-1307
    Electronic ISSN:
    1755-1315
    Topics:
    Geography
    Geosciences
    Physics
    Published by:
    http://www.iop.org/

    Latest Papers from Table of Contents or Articles in Press
  7. 7
    C. R. Webster ; P. R. Mahaffy ; G. J. Flesch ; P. B. Niles ; J. H. Jones ; L. A. Leshin ; S. K. Atreya ; J. C. Stern ; L. E. Christensen ; T. Owen ; H. Franz ; R. O. Pepin ; A. Steele ; C. Achilles ; C. Agard ; J. A. Alves Verdasca ; R. Anderson ; D. Archer ; C. Armiens-Aparicio ; R. Arvidson ; E. Atlaskin ; A. Aubrey ; B. Baker ; M. Baker ; T. Balic-Zunic ; D. Baratoux ; J. Baroukh ; B. Barraclough ; K. Bean ; L. Beegle ; A. Behar ; J. Bell ; S. Bender ; M. Benna ; J. Bentz ; G. Berger ; J. Berger ; D. Berman ; D. Bish ; D. F. Blake ; J. J. Blanco Avalos ; D. Blaney ; J. Blank ; H. Blau ; L. Bleacher ; E. Boehm ; O. Botta ; S. Bottcher ; T. Boucher ; H. Bower ; N. Boyd ; B. Boynton ; E. Breves ; J. Bridges ; N. Bridges ; W. Brinckerhoff ; D. Brinza ; T. Bristow ; C. Brunet ; A. Brunner ; W. Brunner ; A. Buch ; M. Bullock ; S. Burmeister ; M. Cabane ; F. Calef ; J. Cameron ; J. Campbell ; B. Cantor ; M. Caplinger ; J. Caride Rodriguez ; M. Carmosino ; I. Carrasco Blazquez ; A. Charpentier ; S. Chipera ; D. Choi ; B. Clark ; S. Clegg ; T. Cleghorn ; E. Cloutis ; G. Cody ; P. Coll ; P. Conrad ; D. Coscia ; A. Cousin ; D. Cremers ; J. Crisp ; A. Cros ; F. Cucinotta ; C. d'Uston ; S. Davis ; M. Day ; M. de la Torre Juarez ; L. DeFlores ; D. DeLapp ; J. DeMarines ; D. DesMarais ; W. Dietrich ; R. Dingler ; C. Donny ; B. Downs ; D. Drake ; G. Dromart ; A. Dupont ; B. Duston ; J. Dworkin ; M. D. Dyar ; L. Edgar ; K. Edgett ; C. Edwards ; L. Edwards ; B. Ehlmann ; B. Ehresmann ; J. Eigenbrode ; B. Elliott ; H. Elliott ; R. Ewing ; C. Fabre ; A. Fairen ; K. Farley ; J. Farmer ; C. Fassett ; L. Favot ; D. Fay ; F. Fedosov ; J. Feldman ; S. Feldman ; M. Fisk ; M. Fitzgibbon ; M. Floyd ; L. Fluckiger ; O. Forni ; A. Fraeman ; R. Francis ; P. Francois ; C. Freissinet ; K. L. French ; J. Frydenvang ; A. Gaboriaud ; M. Gailhanou ; J. Garvin ; O. Gasnault ; C. Geffroy ; R. Gellert ; M. Genzer ; D. Glavin ; A. Godber ; F. Goesmann ; W. Goetz ; D. Golovin ; F. Gomez Gomez ; J. Gomez-Elvira ; B. Gondet ; S. Gordon ; S. Gorevan ; J. Grant ; J. Griffes ; D. Grinspoon ; J. Grotzinger ; P. Guillemot ; J. Guo ; S. Gupta ; S. Guzewich ; R. Haberle ; D. Halleaux ; B. Hallet ; V. Hamilton ; C. Hardgrove ; D. Harker ; D. Harpold ; A. M. Harri ; K. Harshman ; D. Hassler ; H. Haukka ; A. Hayes ; K. Herkenhoff ; P. Herrera ; S. Hettrich ; E. Heydari ; V. Hipkin ; T. Hoehler ; J. Hollingsworth ; J. Hudgins ; W. Huntress ; J. Hurowitz ; S. Hviid ; K. Iagnemma ; S. Indyk ; G. Israel ; R. Jackson ; S. Jacob ; B. Jakosky ; E. Jensen ; J. K. Jensen ; J. Johnson ; M. Johnson ; S. Johnstone ; A. Jones ; J. Joseph ; I. Jun ; L. Kah ; H. Kahanpaa ; M. Kahre ; N. Karpushkina ; W. Kasprzak ; J. Kauhanen ; L. Keely ; O. Kemppinen ; D. Keymeulen ; M. H. Kim ; K. Kinch ; P. King ; L. Kirkland ; G. Kocurek ; A. Koefoed ; J. Kohler ; O. Kortmann ; A. Kozyrev ; J. Krezoski ; D. Krysak ; R. Kuzmin ; J. L. Lacour ; V. Lafaille ; Y. Langevin ; N. Lanza ; J. Lasue ; S. Le Mouelic ; E. M. Lee ; Q. M. Lee ; D. Lees ; M. Lefavor ; M. Lemmon ; A. Lepinette Malvitte ; R. Leveille ; E. Lewin-Carpintier ; K. Lewis ; S. Li ; L. Lipkaman ; C. Little ; M. Litvak ; E. Lorigny ; G. Lugmair ; A. Lundberg ; E. Lyness ; M. Madsen ; J. Maki ; A. Malakhov ; C. Malespin ; M. Malin ; N. Mangold ; G. Manhes ; H. Manning ; G. Marchand ; M. Marin Jimenez ; C. Martin Garcia ; D. Martin ; M. Martin ; J. Martinez-Frias ; J. Martin-Soler ; F. J. Martin-Torres ; P. Mauchien ; S. Maurice ; A. McAdam ; E. McCartney ; T. McConnochie ; E. McCullough ; I. McEwan ; C. McKay ; S. McLennan ; S. McNair ; N. Melikechi ; P. Y. Meslin ; M. Meyer ; A. Mezzacappa ; H. Miller ; K. Miller ; R. Milliken ; D. Ming ; M. Minitti ; M. Mischna ; I. Mitrofanov ; J. Moersch ; M. Mokrousov ; A. Molina Jurado ; J. Moores ; L. Mora-Sotomayor ; J. M. Morookian ; R. Morris ; S. Morrison ; R. Mueller-Mellin ; J. P. Muller ; G. Munoz Caro ; M. Nachon ; S. Navarro Lopez ; R. Navarro-Gonzalez ; K. Nealson ; A. Nefian ; T. Nelson ; M. Newcombe ; C. Newman ; H. Newsom ; S. Nikiforov ; B. Nixon ; E. Noe Dobrea ; T. Nolan ; D. Oehler ; A. Ollila ; T. Olson ; M. A. de Pablo Hernandez ; A. Paillet ; E. Pallier ; M. Palucis ; T. Parker ; Y. Parot ; K. Patel ; M. Paton ; G. Paulsen ; A. Pavlov ; B. Pavri ; V. Peinado-Gonzalez ; L. Peret ; R. Perez ; G. Perrett ; J. Peterson ; C. Pilorget ; P. Pinet ; J. Pla-Garcia ; I. Plante ; F. Poitrasson ; J. Polkko ; R. Popa ; L. Posiolova ; A. Posner ; I. Pradler ; B. Prats ; V. Prokhorov ; S. W. Purdy ; E. Raaen ; L. Radziemski ; S. Rafkin ; M. Ramos ; E. Rampe ; F. Raulin ; M. Ravine ; G. Reitz ; N. Renno ; M. Rice ; M. Richardson ; F. Robert ; K. Robertson ; J. A. Rodriguez Manfredi ; J. J. Romeral-Planello ; S. Rowland ; D. Rubin ; M. Saccoccio ; A. Salamon ; J. Sandoval ; A. Sanin ; S. A. Sans Fuentes ; L. Saper ; P. Sarrazin ; V. Sautter ; H. Savijarvi ; J. Schieber ; M. Schmidt ; W. Schmidt ; D. Scholes ; M. Schoppers ; S. Schroder ; S. Schwenzer ; E. Sebastian Martinez ; A. Sengstacken ; R. Shterts ; K. Siebach ; T. Siili ; J. Simmonds ; J. B. Sirven ; S. Slavney ; R. Sletten ; M. Smith ; P. Sobron Sanchez ; N. Spanovich ; J. Spray ; S. Squyres ; K. Stack ; F. Stalport ; T. Stein ; N. Stewart ; S. L. Stipp ; K. Stoiber ; E. Stolper ; B. Sucharski ; R. Sullivan ; R. Summons ; D. Sumner ; V. Sun ; K. Supulver ; B. Sutter ; C. Szopa ; F. Tan ; C. Tate ; S. Teinturier ; I. ten Kate ; P. Thomas ; L. Thompson ; R. Tokar ; M. Toplis ; J. Torres Redondo ; M. Trainer ; A. Treiman ; V. Tretyakov ; R. Urqui-O'Callaghan ; J. Van Beek ; T. Van Beek ; S. VanBommel ; D. Vaniman ; A. Varenikov ; A. Vasavada ; P. Vasconcelos ; E. Vicenzi ; A. Vostrukhin ; M. Voytek ; M. Wadhwa ; J. Ward ; E. Weigle ; D. Wellington ; F. Westall ; R. C. Wiens ; M. B. Wilhelm ; A. Williams ; J. Williams ; R. Williams ; R. B. Williams ; M. Wilson ; R. Wimmer-Schweingruber ; M. Wolff ; M. Wong ; J. Wray ; M. Wu ; C. Yana ; A. Yen ; A. Yingst ; C. Zeitlin ; R. Zimdar ; M. P. Zorzano Mier
    American Association for the Advancement of Science (AAAS)
    Published 2013
    Staff View
    Publication Date:
    2013-07-23
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  8. 8
    P. Wang ; S. Zhu ; L. Yang ; S. Cui ; W. Pan ; R. Jackson ; Y. Zheng ; A. Rongvaux ; Q. Sun ; G. Yang ; S. Gao ; R. Lin ; F. You ; R. Flavell ; E. Fikrig
    American Association for the Advancement of Science (AAAS)
    Published 2015
    Staff View
    Publication Date:
    2015-10-24
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Animals ; Caliciviridae Infections/immunology/virology ; Cardiovirus Infections/immunology/virology ; Cytokines/genetics ; Encephalomyocarditis virus/immunology ; Gastroenteritis/immunology/virology ; Gene Expression Regulation ; HEK293 Cells ; Humans ; Immunity, Innate/*genetics ; Interferon Type I/*immunology ; Intestines/*immunology/*virology ; Mice ; Mice, Mutant Strains ; Norovirus/immunology ; RNA Helicases/*physiology ; RNA, Viral/*immunology ; Receptors, Cell Surface/genetics/*physiology ; Ubiquitins/genetics ; Viremia/genetics/immunology
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  9. 9
    Macri, V., Brody, J. A., Arking, D. E., Hucker, W. J., Yin, X., Lin, H., Mills, R. W., Sinner, M. F., Lubitz, S. A., Liu, C.-T., Morrison, A. C., Alonso, A., Li, N., Fedorov, V. V., Janssen, P. M., Bis, J. C., Heckbert, S. R., Dolmatova, E. V., Lumley, T., Sitlani, C. M., Cupples, L. A., Pulit, S. L., Newton-Cheh, C., Barnard, J., Smith, J. D., Van Wagoner, D. R., Chung, M. K., Vlahakes, G. J., ODonnell, C. J., Rotter, J. I., Margulies, K. B., Morley, M. P., Cappola, T. P., Benjamin, E. J., Muzny, D., Gibbs, R. A., Jackson, R. D., Magnani, J. W., Herndon, C. N., Rich, S. S., Psaty, B. M., Milan, D. J., Boerwinkle, E., Mohler, P. J., Sotoodehnia, N., Ellinor, P. T., Cohorts for Heart and Aging Research in Genetic Epidemiology (CHARGE) Sequencing Project, NHLBI Exome Sequencing Project
    American Heart Association (AHA)
    Published 2018
    Staff View
    Publication Date:
    2018-05-16
    Publisher:
    American Heart Association (AHA)
    Print ISSN:
    1942-325X
    Electronic ISSN:
    1942-3268
    Topics:
    Medicine
    Keywords:
    Electrophysiology, Atrial Fibrillation, Ion Channels/Membrane Transport, Functional Genomics
    Published by:
    http://www.americanheart.org/

    Latest Papers from Table of Contents or Articles in Press
  10. 10
    Staff View
    Publication Date:
    2018-08-11
    Publisher:
    The American Society for Pharmacology and Experimental Therapeutics (ASPET)
    Print ISSN:
    0026-895X
    Electronic ISSN:
    1521-0111
    Topics:
    Chemistry and Pharmacology
    Medicine
    Published by:
    http://www.aspet.org/

    Latest Papers from Table of Contents or Articles in Press
  11. 11
    Staff View
    Publication Date:
    2018-05-05
    Publisher:
    American Physical Society (APS)
    Print ISSN:
    0031-9007
    Electronic ISSN:
    1079-7114
    Topics:
    Physics
    Keywords:
    Atomic, Molecular, and Optical Physics
    Published by:
    http://www.aps.org/

    Latest Papers from Table of Contents or Articles in Press
  12. 12
    Blass, P. M. ; Jackson, R. C. ; Polanyi, J. C. ; Weiss, H.

    College Park, Md. : American Institute of Physics (AIP)
    Published 1991
    Staff View
    ISSN:
    1089-7690
    Source:
    AIP Digital Archive
    Topics:
    Physics
    Chemistry and Pharmacology
    Notes:
    The infrared spectra of HBr and HCl on LiF(001) single crystal surfaces were measured as a function of coverage at temperatures ≤83 K using Fourier-transform infrared (FTIR) spectroscopy. For each hydrogen halide three different spectral features could be distinguished. At low coverages broad absorptions centered at 2265±20 cm−1 (HBr) and at 2515±20 cm−1 (HCl) were observed. These absorptions were attributed to molecules hydrogen-bonded to F− anions of the surface, the angle between the molecular axis and the surface being 21±5° for HBr and 19±5° for HCl as determined from experiments employing polarized infrared radiation. Hydrogen bonding was evidenced by: (i) redshifts with respect to the gas phase (∼300 cm−1), (ii) broad infrared absorption (FWHM: 265±25 cm−1 for HBr, 295±15 cm−1 for HCl), and (iii) enhancement of the infrared absorption intensity compared to the gas phase by more than one order of magnitude for both HBr and HCl. With increasing coverage a second absorption was observed before the first one saturated (HBr:ν=2461±5 cm−1, FWHM=75±10 cm−1; HCl: ν=2763±5 cm−1, FWHM=80±10 cm−1). This absorption was attributed to molecules adsorbed in a second layer. The smaller redshift and spectral width for the second layer were consistent with weaker hydrogen bonding, probably to the halogen of molecules adsorbed in the first layer. Further increase in coverage resulted in the appearance of the well-known doublet absorptions due to formation of solid. Coadsorption of HBr and HCl, as well as experiments under adsorption–desorption equilibrium conditions, confirmed that the first and second layers could coexist. The isotherms could best be understood on the assumption of a repulsive interaction within the first layer.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1063/1.460234

    Articles: DFG German National Licenses
  13. 13
    Aloisi, G. ; Barnes, P. ; Catlow, C. R. A. ; Jackson, R. A. ; Richards, A. J.

    College Park, Md. : American Institute of Physics (AIP)
    Published 1990
    Staff View
    ISSN:
    1089-7690
    Source:
    AIP Digital Archive
    Topics:
    Physics
    Chemistry and Pharmacology
    Notes:
    The problem of deriving realistic interaction potentials for modeling hydrated sites within aluminosilicate structures has been considered. It is argued that since a variety of cationic sites is involved, the effect of the lattice must be taken into account explicitly, both in the derivation and in the use of H2O–cation potentials. A new method for doing this is described with examples for H2O–Si4+ and H2O–Al3+ in quartz and/or zeolite-A. A more detailed analysis for H2O–Ca2+ in zeolite-A and cement structures is presented.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1063/1.458789

    Articles: DFG German National Licenses
  14. 14
    Freund, H. P. ; Jackson, R. H. ; Pershing, D. E. ; Taccetti, J. M.

    [S.l.] : American Institute of Physics (AIP)
    Published 1994
    Staff View
    ISSN:
    1089-7674
    Source:
    AIP Digital Archive
    Topics:
    Physics
    Notes:
    A three-dimensional nonlinear formulation of a free-electron laser based upon a coaxial hybrid iron (CHI) wiggler is described. The CHI wiggler is created by insertion of a central rod and an outer ring [composed of alternating ferrite and dielectric spacers in which the ferrite (dielectric) spacer on the central rod is opposite to the dielectric (ferrite) spacer on the outer ring] along the axis of a solenoidal. An analytic model of the CHI wiggler is developed which is in good agreement with the Poisson/Superfish group of codes. The free-electron laser (FEL) formulation is a slow-time-scale analysis of the interaction of an annular electron beam with the CHI wiggler in a coaxial waveguide. The electromagnetic field is represented as the superposition of the vacuum transverse electric (TE), transverse magnetic (TM), and transverse electromagnetic (TEM) modes of the waveguide, and a set of nonlinear second-order differential equations is derived for the amplitudes and phases of these modes. These equations are solved simultaneously with the three-dimensional Lorentz force equations for the combined magnetostatic and electromagnetic fields. An adiabatic taper is used to model the injection of the beam, and an amplitude taper is included for efficiency enhancement. Simulations are presented for Ka-, Ku- and W-band operation. Multimode operation is also studied. The results indicate that operation over a wide bandwidth is practical with the CHI wiggler, and that the bandwidth in the tapered-wiggler cases is comparable to that for a uniform wiggler. Therefore, relatively high field strengths can be achieved with the CHI wiggler at shorter wiggler periods than is possible in many other conventional wiggler designs.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1063/1.870785

    Articles: DFG German National Licenses
  15. 15
    Freund, H. P. ; Jackson, R. H. ; Pershing, D. E. ; Taccetti, J. M.

    [S.l.] : American Institute of Physics (AIP)
    Published 1994
    Staff View
    ISSN:
    1089-7674
    Source:
    AIP Digital Archive
    Topics:
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1063/1.870947

    Articles: DFG German National Licenses
  16. 16
    Freund, H. P. ; Read, M. E. ; Jackson, R. H. ; Pershing, D. E. ; Taccetti, J. M.

    [S.l.] : American Institute of Physics (AIP)
    Published 1995
    Staff View
    ISSN:
    1089-7674
    Source:
    AIP Digital Archive
    Topics:
    Physics
    Notes:
    A G-band free-electron laser designed for plasma heating is described using a coaxial hybrid iron (CHI) wiggler formed by insertion into a solenoid of a central rod and an outer ring of alternating ferrite and nonferrite spacers positioned so that the central ferrite (nonferrite) spacers are opposite the outer nonferrite (ferrite) spacers. The CHI wiggler provides for enhanced beam focusing and the ability to handle intense beams and high-power continuous wave radiation. Simulations indicate that a power/efficiency of 3.5 MW/13% are possible using a 690 kV/40 A beam. No beam loss was found in simulation. © 1995 American Institute of Physics.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1063/1.871323

    Articles: DFG German National Licenses
  17. 17
    Frankel, D. J. ; Fruhberger, B. ; Jackson, R. H. ; Dwyer, D. J.

    [S.l.] : American Institute of Physics (AIP)
    Published 1993
    Staff View
    ISSN:
    1089-7623
    Source:
    AIP Digital Archive
    Topics:
    Physics
    Electrical Engineering, Measurement and Control Technology
    Notes:
    The design of a cold finger and sample holder for use with a long-strong XYZ manipulator in a multitechnique ultrahigh vacuum chamber is presented. The cold finger design, based on the concept of a cryogenic transfer line, minimizes unwanted cryosorption on the surface of the cold finger. The simple, durable design allows samples to be cooled to below 90 K, rapidly heated to above 1000 K, and quickly returned to low temperatures. The rapid heating and cooling rates coupled with low outgassing make the design ideal for thermal desorption spectroscopy studies.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1063/1.1143936

    Articles: DFG German National Licenses
  18. 18
    McKernan, Ruth M. ; Quirk, K. ; Jackson, R. G. ; Ragan, C. Ian

    Oxford, UK : Blackwell Publishing Ltd
    Published 1990
    Staff View
    ISSN:
    1471-4159
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Notes:
    Abstract: 5-Hydroxytryptamine3 (5-HT3) receptors have been identified in the rat brain using the radioligand [3H]Q ICS 205–930. We report here that these sites have been solubilised from membranes prepared from pooled rat cerebral cortex and hippocampus using various detergents. Of the six detergents tested { 1% 3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulphonate, 0.5% deoxycholate, 1% Lubrol, 0.5% digitonin, 1% Triton X-100, and 1% octyl glucoside}, deoxycholate (0.5%) yielded the best solubilisation (54.6 ± 6% of receptor, 70.5 ± 4% of protein; n = 3). However, most detergents inhibited binding of [3H]Q ICS 205–930 in solution. Binding was found to be optimal after the receptor had been exchanged by gel filtration through Sephadex G-25 into the detergent Lubrol PX (0.05%). Binding of [3H]Q ICS 205–930 to these soluble sites was saturable and specific (Bmax, = 46.1 ± 6 fmol/mg of protein; KD= 0.33 ± 0.09 nM; n = 4) and was similar to that observed in membranes. Kinetic studies of [3H]Q ICS 205–930 binding demonstrated it to be rapid, with equilibrium being achieved within 15 min at 4°C. The KD determined from the rates of association and dissociation (0.38 nM) agreed well with that determined by saturation analysis. Various antagonists competed for the soluble receptors with a rank order of potency typical for binding at a 5-HT3 receptor site: zacopride (Ki= 0.26 nM) 〉 quipazine (0.37 nM) = Q ICS 205–930 (0.33 nM) 〉 ICS 205–930 (0.93 nM) 〉 GR 38032F (2.2 nM) 〉 BRL 24924 (4.1 nM) 〉 MDL 72222 (23.4 nM) 〉 ketanserin (6,000 nM). The agonists 5-HT and 2-methyl-5-HT also competed for [3H]Q ICS 205–930 binding with high affinity (39.6 and 55.6 nM respectively). Therefore, we conclude that the 5-HT3 receptor of rat brain has been successfully solubilised, and this should provide a good starting point for purification of the receptor.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1471-4159.1990.tb02339.x

    Articles: DFG German National Licenses
  19. 19
    Jackson, R. L. ; Aprison, M. H.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1966
    Staff View
    ISSN:
    1471-4159
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1471-4159.1966.tb04298.x

    Articles: DFG German National Licenses
  20. 20
    Jackson, R. L. ; Aprison, M. H.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1966
    Staff View
    ISSN:
    1471-4159
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Medicine
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1471-4159.1966.tb04299.x

    Articles: DFG German National Licenses