Search Results - (Author, Cooperation:R. J. Read)
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1Latest Papers from Table of Contents or Articles in PressL. Chen ; M. Kostadima ; J. H. Martens ; G. Canu ; S. P. Garcia ; E. Turro ; K. Downes ; I. C. Macaulay ; E. Bielczyk-Maczynska ; S. Coe ; S. Farrow ; P. Poudel ; F. Burden ; S. B. Jansen ; W. J. Astle ; A. Attwood ; T. Bariana ; B. de Bono ; A. Breschi ; J. C. Chambers ; F. A. Choudry ; L. Clarke ; P. Coupland ; M. van der Ent ; W. N. Erber ; J. H. Jansen ; R. Favier ; M. E. Fenech ; N. Foad ; K. Freson ; C. van Geet ; K. Gomez ; R. Guigo ; D. Hampshire ; A. M. Kelly ; H. H. Kerstens ; J. S. Kooner ; M. Laffan ; C. Lentaigne ; C. Labalette ; T. Martin ; S. Meacham ; A. Mumford ; S. Nurnberg ; E. Palumbo ; B. A. van der Reijden ; D. Richardson ; S. J. Sammut ; G. Slodkowicz ; A. U. Tamuri ; L. Vasquez ; K. Voss ; S. Watt ; S. Westbury ; P. Flicek ; R. Loos ; N. Goldman ; P. Bertone ; R. J. Read ; S. Richardson ; A. Cvejic ; N. Soranzo ; W. H. Ouwehand ; H. G. Stunnenberg ; M. Frontini ; A. Rendon
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-09-27Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: *Alternative Splicing ; Cell Lineage/*genetics ; Genetic Variation ; Hematopoiesis/*genetics ; Hematopoietic Stem Cells/*cytology/metabolism ; Humans ; NFI Transcription Factors/genetics/metabolism ; RNA-Binding Proteins/metabolism ; Thrombopoiesis/genetics ; TranscriptomePublished by: -
2Latest Papers from Table of Contents or Articles in PressR. N. Jackson ; S. M. Golden ; P. B. van Erp ; J. Carter ; E. R. Westra ; S. J. Brouns ; J. van der Oost ; T. C. Terwilliger ; R. J. Read ; B. Wiedenheft
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-08-12Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: CRISPR-Associated Proteins/*chemistry ; *CRISPR-Cas Systems ; *Clustered Regularly Interspaced Short Palindromic Repeats ; Crystallography, X-Ray ; Escherichia coli/*genetics ; Escherichia coli Proteins/*chemistry ; RNA Editing ; RNA, Bacterial/*chemistry ; RNA, Guide/*chemistryPublished by: -
3Latest Papers from Table of Contents or Articles in PressF. DiMaio ; T. C. Terwilliger ; R. J. Read ; A. Wlodawer ; G. Oberdorfer ; U. Wagner ; E. Valkov ; A. Alon ; D. Fass ; H. L. Axelrod ; D. Das ; S. M. Vorobiev ; H. Iwai ; P. R. Pokkuluri ; D. Baker
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-05-03Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Computational Biology/*methods ; Crystallography, X-Ray ; Databases, Protein ; Electrons ; *Models, Molecular ; Proteins/*chemistry ; Sequence Alignment ; Sequence Homology, Amino Acid ; *Structural Homology, ProteinPublished by: -
4Latest Papers from Table of Contents or Articles in PressAzofeifa, J. G., Allen, M. A., Hendrix, J. R., Read, T., Rubin, J. D., Dowell, R. D.
Cold Spring Harbor Laboratory Press
Published 2018Staff ViewPublication Date: 2018-03-06Publisher: Cold Spring Harbor Laboratory PressElectronic ISSN: 1549-5469Topics: BiologyMedicinePublished by: -
5Articles: DFG German National LicensesBast, D. J. ; Banerjee, L. ; Clark, C. ; Read, R. J. ; Brunton, J. L.
Oxford BSL : Blackwell Science Ltd
Published 1999Staff ViewISSN: 1365-2958Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyMedicineNotes: The Verotoxin 1 (VT1) B subunit binds to the glycosphingolipid receptor globotriaosylceramide (Gb3). Receptor-binding specificity is associated with the terminally linked Galα(1–4) Galβ disaccharide sequence of the receptor. Recently, three globotriose (Galα[1–4] Galβ [1–4] Glcβ) binding sites per B-subunit monomer were identified by crystallography. Two of these sites (sites I and II) are located adjacent to phenylalanine-30. Site I was originally predicted as a potential Gb3 binding site on the basis of sequence conservation, and site II was additionally predicted based on computer modelling and receptor docking. The third (site III) was also identified by crystallography and is located at the N-terminal end of the α-helix. To determine the biological significance of sites II and III, and to support our previous findings of the significance of site I, we examined the binding properties and cytotoxicity of VT1 mutants designed to block Gb3 binding at each site selectively. The Scatchard analysis of saturation-binding data for each mutant revealed that only the amino acid substitutions predicted to affect site I (D-17E) or site II (G-62T) caused reductions in the binding affinity and capacity of VT1 for Gb3. Similarly, those mutations at sites I and II also caused significant reductions in both Vero and MRC-5 cell cytotoxicity (by seven and five logs, respectively, for G-62T and by four and two logs, respectively, for D-17E). In contrast, the substitution of alanine for W-34 at site III did not reduce the high-affinity binding of the B subunit, despite causing a fourfold reduction in the receptor-binding capacity. The corresponding mutant W-34A holotoxin had a two-log reduction in cytotoxicity on Vero cells and no statistically significant reduction on MRC-5 cells. We conclude that the high-affinity receptor binding most relevant for cell cytotoxicity occurs at sites I and II. In contrast, site III appears to mediate the recognition of additional Gb3 receptor epitopes but with lower affinity. Our results support the significance of the indole ring of W-34 for binding at this site.Type of Medium: Electronic ResourceURL: