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1Latest Papers from Table of Contents or Articles in PressF. Denoeud ; L. Carretero-Paulet ; A. Dereeper ; G. Droc ; R. Guyot ; M. Pietrella ; C. Zheng ; A. Alberti ; F. Anthony ; G. Aprea ; J. M. Aury ; P. Bento ; M. Bernard ; S. Bocs ; C. Campa ; A. Cenci ; M. C. Combes ; D. Crouzillat ; C. Da Silva ; L. Daddiego ; F. De Bellis ; S. Dussert ; O. Garsmeur ; T. Gayraud ; V. Guignon ; K. Jahn ; V. Jamilloux ; T. Joet ; K. Labadie ; T. Lan ; J. Leclercq ; M. Lepelley ; T. Leroy ; L. T. Li ; P. Librado ; L. Lopez ; A. Munoz ; B. Noel ; A. Pallavicini ; G. Perrotta ; V. Poncet ; D. Pot ; Priyono ; M. Rigoreau ; M. Rouard ; J. Rozas ; C. Tranchant-Dubreuil ; R. VanBuren ; Q. Zhang ; A. C. Andrade ; X. Argout ; B. Bertrand ; A. de Kochko ; G. Graziosi ; R. J. Henry ; Jayarama ; R. Ming ; C. Nagai ; S. Rounsley ; D. Sankoff ; G. Giuliano ; V. A. Albert ; P. Wincker ; P. Lashermes
American Association for the Advancement of Science (AAAS)
Published 2014Staff ViewPublication Date: 2014-09-06Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Caffeine/biosynthesis/*genetics ; Coffea/classification/*genetics ; *Evolution, Molecular ; *Genome, Plant ; Methyltransferases/genetics/*physiology ; Phylogeny ; Plant Proteins/genetics/*physiologyPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 1471-0528Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1471-0528Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesHENRY, R. J. W. ; GOODMAN, J. D. S. ; GODLEY, M. ; RAJU, K. S. ; COFFER, A. I. ; KING, R. J. B.
Oxford, UK : Blackwell Publishing Ltd
Published 1988Staff ViewISSN: 1471-0528Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Summary. Using a monoclonal antibody raised against an oestrogen receptor-related protein, p29, and an indirect immunoperoxidase technique to stain human tissue, the presence of the antigen was investigated in normal, dysplastic and malignant tissue of the uterine cervix. In normal tissue p29 was present throughout the ectocervix during the menstrual cycle and virtually absent from the endocervix. In dysplastic cervical tissue there was a decreasing p29 content with increasing severity of the dysplasia, and very low levels were seen in the carcinomatous tissues.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1439-0523Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: The carbohydrate content of Hordeum spotaneum lines collected from 23 diverse environments in Israel was investigated. The range in composition was sucrose: 0.83—1.15%; trisaccharides): 0.48—1.16% total free and combined fructose: 1.2—4.2%; pentosans: 4.2—9.9%; and fl→3), (1→4)-β-glucans: 4.5—13.2%. The high pentosan and β-glucan content in some lines would cause quality problems if found in malting barleys.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1573-093XSource: Springer Online Journal Archives 1860-2000Topics: PhysicsNotes: Abstract Recent R-matrix calculations of electron impact excitation rates in Fe xii are used to derive the theoretical emission line ratio R 1 = I(195.1 Å)/I(1242 Å), which is potentially a useful electron density diagnostic for the solar inner corona (r ≤ 1.05 61-01). These results are found to be significantly different from the earlier estimates of Withbroe and Raymond (1984), but are in good agreement with the observed values of R 1, for the quiet Sun and an active region. Adoption of the R-matrix atomic data for the 1242 Å line in the coronal iron abundance determination removes an existing discrepancy between results derived from the EUV transition and other iron lines in the solar XUV spectrum. The R-matrix calculations confirm the prediction of Withbroe and Raymond that the earlier discrepancies in R 1 and the iron abundance were due to the 1242 Å line excitation rates being underestimated by a factor of ~2. Withbroe and Raymond's paper is, therefore, an excellent example of how astronomical observations can be used to accurately predict atomic physics data.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesScott, K. D. ; Eggler, P. ; Seaton, G. ; Rossetto, M. ; Ablett, E. M. ; Lee, L. S. ; Henry, R. J.
Springer
Published 2000Staff ViewISSN: 1432-2242Keywords: Key words ESTs ; Microsatellites ; Vitis viniferaSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract One hundred and twenty four microsatellites were isolated from analysis of 5000 Vitis expressed sequence tags (ESTs). A diversity of dinucleotide and trinucleotide simple sequence repeat (SSR) motifs were present. Primers were designed for 16 of these SSRs and they were tested on seven accessions. Ten of the sixteen primer pairs resulted in PCR products of the expected size. All ten functional primers were polymorphic across the accessions studied. Polymorphisms were evident at the level of cultivars, Vitis species, and between related genera. SSRs that were from the 3′ untranslated region (3′UTR) were most polymorphic at the cultivar level, the 5′ untranslated region (5′ UTR) SSRs were most polymorphic between cultivars and species, and those SSRs within coding sequence were most polymorphic between species and genera. These results show that EST-derived SSRs in Vitis are useful as they are polymorphic and highly transferable. With EST SSRs being applicable to studies at several taxonomic levels, the large number of SSRs (approximately 1000) that will be available from an expanded EST database of 45 000 will have many potential applications in mapping and identity research.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesTaylor, P. W. J. ; Geijskes, J. R. ; Ko, H.-L. ; Fraser, T. A. ; Henry, R. J. ; Birch, R. G.
Springer
Published 1995Staff ViewISSN: 1432-2242Keywords: RAPD ; Sugarcane ; Embryogenic callus ; Genetic transformation ; Somaclonal variationSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Random amplified polymorphic DNA (RAPD) analysis using 10-mer oligonucleotide primers efficiently differentiated sugarcane cultivars and proved suitable for detecting gross genetic change such as that which can occur in sugarcane subjected to prolonged tissue culture, for example in protoplast-derived callus. However, RAPD analysis was not sufficiently sensitive to detect smaller genetic changes that occur during sugarcane genetic transformation. The length of DNA scored for polymorphism per primer averaged 13.2 kb, or 0.0001% of the typical sugarcane genome size of 1.2 × 107 kb (2C). RAPD analysis of sugarcane plants regenerated from embryogenic callus revealed very few polymorphisms, indicating that gross genetic change is infrequent during this tissue culture procedure, although epigenetic effects result in transient morphological changes in regenerated plants. More sensitive variations on the RAPD technique may increase the practicality of DNA-based screening of regenerated plant lines to reveal somaclonal variants.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesPoulsen, D. M. E. ; Henry, R. J. ; Johnston, R. P. ; Irwin, J. A. G. ; Rees, R. G.
Springer
Published 1995Staff ViewISSN: 1432-2242Keywords: Barley ; Puccinia hordei ; Resistance genes ; Bulk segregant analysis ; Polymerase chain reactionSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract An F2 population from a cross between barley accession Q21861 and the Australian barley variety ‘Galleon’ was used to develop RAPD markers for resistance to barley leaf rust (Puccinia hordei). Resistant and susceptible DNA bulks were constructed following the classification of F2 plants by leaf rust infection type. Bulked segregant analysis was then used to identify a 2.7-kb marker, designated OU022700 and located approximately 12cM from RphQ, a leaf rust resistance gene in Q21861. The marker was generated by PCR with the oligonucleotide primer OPU-02 (Operon). Infection types of F3 progeny were used to confirm assignment of F2 genotypes. OU022700 was shown, retrospectively, to be useful in the identification of individual F2 plants that had been originally misclassified as having susceptible infection types. Both the RAPD marker and RphQ will be potentially useful in the development of new barley cultivars.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1432-2242Keywords: α-amylase ; Barley ; DNA polymorphism ; Evolution ; Polymerase chain reactionSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract α-Amylases are the key enzymes involved in the hydrolysis of starch in plants. The polymerase chain reaction (PCR) was used to detect polymorphisms in the length of amplified sequences between the annealing sites of two primers derived from published α-amy1 gene sequences in barley. These two primers (Bsw1 and Bsw7), flanking the promoter region and the first exon, amplified two PCR fragments in barley. One of the amplified products, with the expected length of 820 bp, appeared together with another shorter PCR band of around 750 bp. This 750-bp fragment seems to be derived from an α-amylase gene not reported previously. Both of the PCR products could be amplified from the two-rowed barley varieties tested, including cv Himalaya from which the sequence information was obtained. Five of the six-rowed barley varieties also have the two PCR fragments whereas another two have only the long fragment. These two fragments seem to be unique to barley, neither of them could be amplified from other cereals; for example, wheat, rye or sorghum. These two α-amylase fragments were mapped to the long arm of 6H, the location of the α-amy1 genes, using wheat-barley addition lines. Amplification of genomic DNA from wild barley accessions with primers Bsw1 and Bsw7 indicated that both of the fragments could be present, or the long and short fragments could be present alone. The results also demonstrated that the genes specifying these two fragments could be independent from each other in barley. The conserved banding pattern of these two fragments in the two-rowed barley varieties implies that artificial selection from these genes may have played an important role in the evolution of cultivated barley from wild barley.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesRossetto, M. ; McLauchlan, A. ; Harriss, F. C. L. ; Henry, R. J. ; Baverstock, P. R. ; Lee, L. S. ; Maguire, T. L. ; Edwards, K. J.
Springer
Published 1999Staff ViewISSN: 1432-2242Keywords: Key words Simple sequence repeats ; Enriched microsatellite library ; Molecular marker ; Myrtaceae ; MelaleucaSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract The sequencing of 831 clones from an enriched microsatellite library of Melaleuca alternifolia (Myrtaceae) yielded 715 inserts containing repeat motifs. The majority of these (98%) were dinucleotide repeats or trinucleotide repeats averaging 22 and 8 repeat motifs respectively. The AG/GA motif was the most common, accounting for 43% of all microsatellites. From a total of 139 primer pairs designed, 102 produced markers within the expected size range. The majority of these (93) were polymorphic. Primer pairs were tested on five selected M. alternifolia genotypes. Loci based on dinucleotide repeats detected on average a greater number of alleles (4.2) than those based on trinucleotide repeats (2.9). The loci described will provide a large pool of polymorphisms useful for population studies, genetic mapping, and possibly application in other Myrtaceae.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 1573-5060Keywords: Hordeum vulgare ; barley ; malting quality ; assessmentSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary Methods for assessing malting quality in barley breeding were evaluated for their precision, including variation between different batches, analysts and barley samples. The barley characters measured were grain moisture, ground grain moisture (two methods), grain weight, steep moisture, malt moisture (two methods), malt yield, malt nitrogen, malt soluble nitrogen, Kolbach index, malt diastatic power, malt α-amylase, malt β-glucanase, wort refractive index, hot water extract, extract yield, wort reducing sugars, wort total carbohydrates, wort α-amino nitrogen and wort total nitrogen. The value of these measurements in barley breeding is discussed.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1573-5060Keywords: Triticum aestivum ; wheat ; dormancy ; gibberellic acid ; pre-harvest sproutingSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary Gibberellic acid, cold (4°C) and a combination of these two treatments were tested for use in breaking dormancy in 27 lines of white-grained wheat with varying levels of resistance to pre-harvest sprouting. Germination increased in all lines treated with gibberellic acid. Dormancy could be broken with 1 μM gibberellic acid. Response to cold varied. A combination of gibberellic acid and cold treatment was the most effective. This technique has been found useful in treating seed in a breeding program aimed at producing wheats with resistance to pre-harvest sprouting.Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesKo, H. L. ; Cowan, D. C. ; Henry, R. J. ; Graham, G. C. ; Blakeney, A. B. ; Lewin, L. G.
Springer
Published 1994Staff ViewISSN: 1573-5060Keywords: genetic diversity ; polymerase chain reaction ; rice ; random amplified polymorphic DNA ; Oryza sativa variationSource: Springer Online Journal Archives 1860-2000Topics: Agriculture, Forestry, Horticulture, Fishery, Domestic Science, NutritionNotes: Summary The genetic relationships between rice varieties were analysed by using the polymerase chain reaction (PCR), with arbitrary oligonucleotide primers in the random amplified polymorphic DNA (RAPD) method. PCR with 22 arbitrary primers applied to 37 varieties produced 144 useful markers, of which 67% were polymorphic. Thus, with selected primers sufficient polymorphism could be detected to allow identification of individual varieties. Visual examination of electrophoresis gels and analysis of banding patterns confirmed that commercial Australian and USA lines and their relatives were very closely related, with similarity indices of 88–97%. Three varieties originating from more distant geographical centres were easily distinguished, producing variety-specific amplification profiles and expressing a lower similarity index of 80% to all other varieties tested. PCR offers a potentially simple, rapid and reliable method for rice genotype identification and recognition of lines that could contribute genetic diversity to new commercial varieties.Type of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 1573-093XSource: Springer Online Journal Archives 1860-2000Topics: PhysicsNotes: Abstract New theoretical electron-density-sensitive Fe xii emission line ratios $$R_1 = I(3s^2 3p^3 {}^4S_{3/2} - 3s3p^4 {}^4P_{5/2} )/I(3s^2 3p^3 {}^2P_{3/2} - 3s3p^4 D_{5/2} )$$ and $$R_2 = I(3s^2 3p^3 {}^2P_{3/2} - 3s3p^4 {}^2D_{5/2} )/I(3s^2 3p^3 {}^4S_{3/2} - 3s3p^2 P_{3/2} )$$ are derived using R-matrix electron impact excitation rate calculations. We have identified the Fexii $$3s^2 3p^3 {}^4S_{3/2} - 3s3p^4 {}^4P_{5/2} ,{\text{ }}3s^2 3p^3 {}^2P_{3/2} - 3s^3 3p^4 {}^2D_{5/2} ,{\text{ }}3s^2 3p^3 S_{3/2} - 3s^2 3p^3 P_{3/2} $$ and $$3s^2 3p^3 {}^4S_{3/2} - 3s^2 3p^3 {}^2P_{1/2}$$ transitions in an active region spectrum obtained with the Harvard S-055 spectrometer on board Skylab at wavelengths of 364.0, 382.8, 1241.7, and 1349.4 Å, respectively. Electron densities determined from the observed values of R 1 (log N e ≃ 11.0) and R 2(log N e ≃ 11.4) are significantly larger than the typical active region measurements, but are similar to those derived from some active region spectra observed with the Skylab 2082A instrument, which provides observational support for the atomic data adopted in the line ratio calculations, and also for the identification of the Fe xii transitions in the S-055 spectrum. However the observed value of R 3 = I(1349.4 Å)/I(1241.7 Å) is approximately a factor of two larger than one would expect from theory which, considering that the 1349.4 Å line lies at the edge of the S-055 wavelength coverage, may reflect errors in the instrument efficiency curve. Another possibility is that the 1349.4 Å transition is blended, probably with Si ii 1350.1 Å.Type of Medium: Electronic ResourceURL: