Search Results - (Author, Cooperation:R. Hurwitz)

2014-08-15

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Anti-Bacterial Agents/metabolism ; Basic Helix-Loop-Helix Transcription Factors/*metabolism ; Bone Marrow Cells/cytology ; Cytokines/immunology/metabolism ; Feedback, Physiological ; Humans ; Ligands ; Macrophage Activation ; Mice ; Mycobacterium tuberculosis/growth & development/*immunology/metabolism ; Phenazines/metabolism ; Pigments, Biological/chemistry/*metabolism ; Pseudomonas Infections/metabolism ; Pseudomonas aeruginosa/*immunology/metabolism ; Pyocyanine/metabolism ; Receptors, Aryl Hydrocarbon/*metabolism ; Receptors, Pattern Recognition/*metabolism ; Virulence Factors/chemistry/metabolism

1471-4159

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Abstract: To determine the presence of cone or rod cyclic GMP phosphodiesterase (EC 3.1.4.17) in the mammalian pineal, extracts from adult rat and bovine pineals were injected onto a Mono Q anion-exchange HPLC column and eluted with an NaCl linear gradient. Fractions were immunoadsorbed with monoclonal antibodies specific to rod and cone phosphodiesterases (ROS-1) and to calmodulin-phosphodiesterase complexes (ACC). Profiles were assayed with 10 µmol/L [3H]cyclic GMP in the presence of calcium-calmodulin, histone, or trypsin. Rat and bovine pineals displayed a single peak of activity recognized by ROS-1, which corresponded to the activity of the cone but, not to the rod in bovine retina. ROS-1 immunoadsorbed ∼80% of the activity in the 60-day-old rat pineal but only 26% of the activity in bovine pineal. ACC immunoadsorbed the remaining activity in both species. Western blot analysis of rat pineal extracts revealed three polypeptides of ∼87, 15, and 10 kDa when probed with a rod/cone phosphodiesterase-specific antiserum. The specific activity of the cone-like phosphodiesterase in 10-day-old rat pineals was twice that of this isozyme in the bovine retina and 150 times that in the bovine pineal. The specific activity of phosphodiesterase in rat pineals decreased with age. We conclude that an enzyme with biochemical and antigenic characteristics similar to cone, but distinct from rod phosphodiesterase, is present in bovine and rat pineals.

Electronic Resource

1600-0560

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Since many studies have shown the discordant diagnoses of melanocytic lesions among pathologists despite the existence of an established histologic criteria, we sought to refine the histologic criteria by finding an additional reliable and reproducible objective histopathologic feature, to aid in the diagnosis of melanoma in situ. We performed a retrospective analysis of 100 cases, histologically diagnosed as melanoma in situ and compared to a study control group consisting of junctional benign melanocytic nevi. The epidermis of all the melanocytic lesions were examined for epidermal effacement. Examination of the epidermis in the study group revealed absence of epidermal effacement in only seven (10%) cases, whereas ninety-three (93%) cases showed absence of rete ridges in some foci, making this an overwhelming majority finding in the cases examined. These results serve as a compelling adjunctive finding that can be used to increase the histologic diagnostic accuracy of melanoma in situ.

Electronic Resource

0006-291X

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0006-291X

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

0006-291X

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Biology

Chemistry and Pharmacology

Physics

Electronic Resource

1432-1971

Key words: Eustachian valve — Cor triatriatum dexter

Springer Online Journal Archives 1860-2000

Medicine

Abstract. Although commonly found on echocardiograms, persistent Eustachian valves rarely result in clinical symptoms in newborns and infants without other significant structural heart disease. Previous reports have suggested that such patients require surgical treatment. The goal of this study is to delineate better the clinical course in infants presenting with symptoms that are a result of persistent Eustachian valves. A review of all patients with symptoms attributed to persistent Eustachian valves over a 10-year period at Riley Hospital for Children showed 3 of 4 patients improved, with eventual resolution of symptoms, with conservative management alone. This suggests that, contrary to previous reports, clinical observation may be warranted in many of these infants.

Electronic Resource

1432-1807

Springer Online Journal Archives 1860-2000

Mathematics

Electronic Resource

1435-604X

Vascular specific injury ; Pulsed dye laser ; Multiple pulses ; Port-wine stain

Springer Online Journal Archives 1860-2000

Medicine

Physics

Technology

Abstract The pulsed dye laser was developed for the treatment of vascular cutaneous lesions using single non-overlapping pulses. However, in practice, overlap and multiple pulses to the same site are commonly used. This study was undertaken to examine the effects of single vs two, three and five pulses to the same site on penetration depth of the laser beam and vascular selectivity. Two albino pigs were treated with a 577 nm, 450μs, 3 mm-diameter pulsed dye laser. The effect of multiple pulses to the same site over a range of fluences was analysed histologically from biopsies acquired immediately and at 1 and 4 days after laser irradiation. The results show that multiple pulses to the same site do not increase the penetration depth (maximum depth from the dermo-epidermal junction where damaged blood vessels are observed) of irreversible vascular injury. Cumulative pulses do increase the extent of tissue injury, including both desired vascular changes and unwanted effects such as extravasation. No clinical changes were observed following multiple pulses below 2 J cm−2. It was concluded that 577 nm, 450μs multiple pulses to the same site do not increase penetration depth of vascular injury of the laser beam.

Electronic Resource