Search Results - (Author, Cooperation:R. Hovius)
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1Latest Papers from Table of Contents or Articles in PressCook, K. L., Andermann, C., Gimbert, F., Adhikari, B. R., Hovius, N.
American Association for the Advancement of Science (AAAS)
Published 2018Staff ViewPublication Date: 2018-10-05Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyGeosciencesComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Geochemistry, GeophysicsPublished by: -
2Latest Papers from Table of Contents or Articles in PressG. Hassaine ; C. Deluz ; L. Grasso ; R. Wyss ; M. B. Tol ; R. Hovius ; A. Graff ; H. Stahlberg ; T. Tomizaki ; A. Desmyter ; C. Moreau ; X. D. Li ; F. Poitevin ; H. Vogel ; H. Nury
Nature Publishing Group (NPG)
Published 2014Staff ViewPublication Date: 2014-08-15Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Amino Acid Sequence ; Animals ; Binding Sites ; Crystallography, X-Ray ; Mice ; Models, Molecular ; Molecular Sequence Data ; Neurotransmitter Agents/metabolism ; Protein Structure, Quaternary ; Protein Structure, Tertiary ; Protein Subunits/chemistry/metabolism ; Receptors, Serotonin, 5-HT3/*chemistry/metabolismPublished by: -
3Articles: DFG German National LicensesStaff View
ISSN: 0005-2736Keywords: (Rat liver) ; Cardiolipin ; Digitonin ; Inner mitochondrial membrane ; Mitochondrion, isolation ; Mitochondrion, subfractionation ; Outer mitochondrial membrane ; Phospholipid compositionSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 0005-2736Keywords: (Rat liver) ; Inner mitochondrial membrane ; Mitochondrion, isolated ; Outer mitochondrial membrane ; Phosphatidylcholine transfer protein ; Phospholipid composition ; Transfer proteinSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 0005-2728Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesRojo, M. ; Hovius, R. ; Demel, R. ; Wallimann, T. ; Eppenberger, H.M. ; Nicolay, K.
Amsterdam : ElsevierStaff ViewISSN: 0014-5793Keywords: Creatine kinase ; Mitochondrion ; Model membrane ; Protein-lipid interactionSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 0014-5793Keywords: Cardiolipin ; Mitochondrion ; Outer membrane ; Phosphatidyleholine transfer protein ; Phospholipase A"2 ; Phospholipid asymmetry ; Rat liverSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesBlasey, H. D. ; Brethon, B. ; Hovius, R. ; Vogel H, H. ; Tairi, A. P. ; Lundström, K. ; Rey, L. ; Bernard, A. R.
Springer
Published 2000Staff ViewISSN: 1573-0778Keywords: 5-HT3 receptor ; large scale ; reactor ; Semliki Forest virus ; suspension process ; transient expressionSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineProcess Engineering, Biotechnology, Nutrition TechnologyNotes: Abstract The expression of recombinant proteins with the Semliki Forest Virus (SFV) system has been scaled up to bioreactor scale. As a model protein for this study the human 5-HT3 receptor was chosen. The gene for the receptor was subcloned into the SFV expression plasmid pSFV1. Virus production by in vivo packaging and production of the recombinant protein was scaled up, the latter to a reactor volume of 11.5 l. A VibromixTM agitation system was chosen to overcome aggregation problems of BHK cells in suspension. In the process, cells were first grown to a density of 106 cells/ml, the medium was then exchanged with fresh medium and the culture was infected with the recombinant virus at an estimated multiplicity of infection of 30. 24 h post infection we measured an expression level of 3 million functional 5-HT3 receptors per cell. For harvesting, the cells were pelleted by centrifugation. The receptor protein was purified in a single step (Hovius et al., 1998) by exploiting the hexa-His tag at minimal protein loss (51% yield). Experiments to optimise expression resulted in yields up to 8 million receptors per cell, when the pH of a suspension culture was controlled at pH 7.3. Rapid virus generation and protein production, high protein yields as well as successful large scale application have made the SFV expression system attractive to produce large quantities of recombinant protein in a very short time. After optimisation of the expression conditions (in particular by setting the pH at 7.3), yields were increased twofold.Type of Medium: Electronic ResourceURL: