Search Results - (Author, Cooperation:R. C. Brown)

2018-05-03

American Physical Society (APS)

0031-9007

1079-7114

Physics

Atomic, Molecular, and Optical Physics

2015-05-02

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

2012-01-27

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

2012-08-21

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Antigens, Bacterial/chemistry/genetics/metabolism ; Apoptosis Regulatory Proteins/deficiency/metabolism ; Bacterial Toxins/chemistry/genetics/metabolism ; Body Fluids/metabolism ; Body Temperature ; Calcium Signaling ; Calcium-Binding Proteins/deficiency/metabolism ; Capillary Permeability ; Caspase 1/deficiency/metabolism ; Cyclooxygenase 1/deficiency ; Cytosol/metabolism ; Death ; Eicosanoids/*biosynthesis/metabolism ; Female ; Flagellin/genetics/immunology/metabolism ; Fluid Shifts ; Hematocrit ; Immunity, Innate/immunology ; Inflammasomes/*metabolism ; Inflammation/immunology/metabolism/pathology ; Interleukin-18 ; Interleukin-1beta ; Intestines/metabolism ; Legionella pneumophila ; Macrophages, Peritoneal/immunology ; Male ; Mice ; Mice, Inbred C57BL ; Neuronal Apoptosis-Inhibitory Protein/deficiency/metabolism ; Peritoneal Cavity ; Peritoneal Lavage ; Recombinant Fusion Proteins/genetics/metabolism ; Salmonella Infections/immunology ; Salmonella typhimurium/immunology ; Time Factors

1077-3118

AIP Digital Archive

Physics

We report experimental and theoretical evidence for electrostatic lateral confinement induced by a nanoprobe. The lateral confinement is manifest as oscillations of the differential conductance of a near-surface resonant-tunneling heterostructure in air at room temperature. © 1995 American Institute of Physics.

Electronic Resource

1750-3841

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Process Engineering, Biotechnology, Nutrition Technology

A method for determination of the filterability of wine was developed. The method is based on evaluation of the filtration curve of a wine filtered through a membrane disc 13 mm in diameter, having a pore size of 0.45 micron. Results of the filterability test were found to be related to plant operation. According to the method developed, a wine is ready for membrane filtration the moment that its filterability test indicates that the differential filtered volume remains unchanged for most of the 60 min determination time. A decrease in the differential filtered volume indicates that the wine contains insolubles/microorganisms, and the rate of the decrease should be considered to be proportional to the load on the membrane. This method is not limited to the wine industry, but can be adapted and/or modified for similar applications, i.e., pharmaceuticals, breweries, fine chemicals.

Electronic Resource

1550-7408

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Exponentially dividing culture forms of Trypanosoma brucei did not utilize glucose provided in the culture medium. The inclusion of 2-deoxyglucose in the medium had no effect on the growth of the trypanosomes. Glucose could be replaced by proline in the liquid phase of biphasic medium without affecting the doubling time of the organisms. Proline added to the culture medium in this way disappeared during the log phase of growth. Glucose in the culture medium was used by the trypanosomes only when the stationary growth phase had been reached. Lipid accumulated in stationary phase trypanosomes grown in glucose-containing medium, but there was no lipid accumulation in log phase organisms or in those which had been grown in proline-containing medium. Bloodstream trypanosomes transferred to liquid medium rapidly utilized glucose over the first 12 hr of culture, and this was accompanied by an accumulation of free pyruvate in the medium. The rate of glucose utilization fell off over the next 36 hr; this was accompanied by a lowering of free pyruvate in the medium and a rise in the proline oxidase activity of the trypanosomes. The possible biologic significance of proline to trypanosomes developing in the midgut of the tsetse vector is discussed.

Electronic Resource

1550-7408

Blackwell Publishing Journal Backfiles 1879-2005

Biology

SYNOPSIS. Diphenylamine was shown to be a potent inhibitor of cyanide insensitive respiration in both bloodstream and newly established culture forms of the same isolate of Trypanosoma brucei, with the L-α-glycerophosphate oxidase system having the greatest sensitivity to the inhibitor. The NADH oxidase activity of bloodstream forms was at least twice as sensitive to diphenylamine as the corresponding activity in culture forms, suggesting different routes of NADH oxidation in the 2 forms. The oxidation of L-α-glycerophosphate was inhibited to a similar degree in both culture and bloodstream forms. L-α-glycerophosphate oxidation in bloodstream forms differed from that found in culture forms in that the bloodstream system, unlike that in the culture form, was unable to donate electrons to cytochrome c. In culture form trypanosomes there was a distinct difference in the degree of diphenylamine inhibition on the oxidation of L-α-glycerophosphate, NADH, and succinate, suggesting the participation of separate flavoproteins in the oxidation of these substrates.

Electronic Resource

1550-7408

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Cyanide-insensitive respiration of bloodstream and culture forms of T. brucei was inhibited by m-chlorobenz-hydroxamic acid (m-CLAM). The cyanide-sensitive respiration of culture forms of this organism was not affected by m-CLAM. This compound is the 1st really effective inhibitor to be described that acts specifically on the cyanide-insensitive respiration of the T. brucei group of trypanosomes; as such it may be of some importance as a trypanocidal drug.Evidence is adduced which suggests that a branched electron transport chain is present in culture forms of T. brucei and that the cyanide-insensitive terminal oxidase found in these stages is the same as that found in bloodstream forms.

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] We describe here a culture form of Trypanosoma brucei which develops during the transformation qf bloodstreain forms into established culture forms and differs from either; of these forms in its respiratory metabolism. Rats infected with a pleomorphic strain of T. brucei were bled 1 day after the ...

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] When horse erythrocytes were mixed with logarithmic phase cultures of A. castellanii, massive mixed-cell agglutination resulted. Such a reaction was absent in mixtures of amoebae with human (all ABO groups) or sheep red cells. A range of carbohydrates were tested for their ability to inhibit this ...

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] Ultrasonic expansion techniques are being tested in a small helium chamber at ...

Electronic Resource

1476-4687

Nature Archives 1869 - 2009

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

[Auszug] MONEL metal is an alloy containing approximately 67 per cent nickel, 28 per cent copper, and 5 per cent other metals, which is made from a natural ore mined in Ontario, Canada. It is of great utility in cases where resistance to corrosion is important. Its chief properties and commercial uses ...

Electronic Resource

1432-0800

Springer Online Journal Archives 1860-2000

Energy, Environment Protection, Nuclear Power Engineering

Medicine

Electronic Resource

0037-7996

History

1432-0592

Springer Online Journal Archives 1860-2000

Architecture, Civil Engineering, Surveying

Geography

Economics

Electronic Resource

1420-908X

Springer Online Journal Archives 1860-2000

Medicine

Abstract A macrophage-like cell line (P388D1) has been treated with asbestos and the release of arachidonic acid and its metabolites has been studied using two methods. In the first monolayer cultures of the cells were labelled with tritiated arachidonic acid and the release of label into the medium was quantified: secondly the synthesis and release of prostaglandins E2 and F2α were followed using radioimmune assay. Crocidolite asbestos caused the greatest release of tritium while the medium from chrysotile-treated cultures contained more of both prostaglandins. Both of the fibrous dusts were significantly more active in both test systems than were the two ‘inert’ materials — titanium dioxide and milled sample of crocidolite. It is suggested that these phenomena are due to the effect of mineral dusts on phospholipase activity and that differences in this activity are associated with differences in the pathogenicity of various mineral dusts.

Electronic Resource

1432-2145

Key words Arabidopsis thaliana ; Alveoli ; Development ; Endosperm ; Microtubules ; Seeds

Springer Online Journal Archives 1860-2000

Biology

Abstract  The process of endosperm development in Arabidopsis was studied using immunohistochemistry of tubulin/microtubules coupled with light and confocal laser scanning microscopy. Arabidopsis undergoes the nuclear type of development in which the primary endosperm nucleus resulting from double fertilization divides repeatedly without cytokinesis resulting in a syncytium lining the central cell. Development occurs as waves originating in the micropylar chamber and moving through the central chamber toward the chalazal tip. Prior to cellularization, the syncytium is organized into nuclear cytoplasmic domains (NCDs) defined by nuclear-based radial systems of microtubules. The NCDs become polarized in axes perpendicular to the central cell wall, and anticlinal walls deposited among adjacent NCDs compartmentalize the syncytium into open-ended alveoli overtopped by a crown of syncytial cytoplasm. Continued centripetal growth of the anticlinal walls is guided by adventitious phragmoplasts that form at interfaces of microtubules emanating from adjacent interphase nuclei. Polarity of the elongating alveoli is reflected in a subsequent wave of periclinal divisions that cuts off a peripheral layer of cells and displaces the alveoli centripetally into the central vacuole. This pattern of development via alveolation appears to be highly conserved; it is characteristic of nuclear endosperm development in angiosperms and is similar to ancient patterns of gametophyte development in gymnosperms.

Electronic Resource

1432-2145

Cytokinesis ; Microtubules ; Microsporogenesis ; Orchids ; Phragmoplast ; Pollen

Springer Online Journal Archives 1860-2000

Biology

Abstract Microsporocytes of the slipper orchidCypripedium californicum A. Gray divide simultaneously after second meiosis. The organization and apportionment of the cytoplasm throughout meiosis are functions of nuclear-based radial microtubule systems (RMSs) that define domains of cytoplasm - a single sporocyte domain before meiosis, dyad domains within the undivided cytoplasm after first meiosis, and four spore domains after second meiosis. Organelles migrate to the interface of dyad domains in the undivided cytoplasm after first meiotic division, and second meiotic division takes place simultaneously on both sides of the equatorial organelle band. Microtubules emanating from the telophase II nuclei interact to form columnar arrrays that interconnect all four nuclei, non-sister as well as sister. Cell plates are initiated in these columns of microtubules and expand centrifugally along the interface of opposing RMSs, coalescing in the center of the sporocyte and joining with the original sporocyte wall at the periphery to form the tetrad of microspores. Organelles are distributed into the spore domains in conjunction with RMSs. These data, demonstrating that cytokinesis in microsporogenesis can occur in the absence of both components of the typical cytokinetic apparatus (the preprophase band of microtubules which predicts the division site and the phragmoplast which controls cell-plate deposition), suggest that plant nuclei have an inherent ability to establish a domain of cytoplasm via radial microtubule systems and to regulate wall deposition independently of the more complex cytokinetic apparatus of vegetative cells.

Electronic Resource

1432-2145

Key words Cytokinesis ; Microtubules ; Microsporogenesis ; Orchids ; Phragmoplast ; Pollen

Springer Online Journal Archives 1860-2000

Biology

Abstract  Microsporocytes of the slipper orchid Cypripedium californicum A. Gray divide simultaneously after second meiosis. The organization and apportionment of the cytoplasm throughout meiosis are functions of nuclear-based radial microtubule systems (RMSs) that define domains of cytoplasm – a single sporocyte domain before meiosis, dyad domains within the undivided cytoplasm after first meiosis, and four spore domains after second meiosis. Organelles migrate to the interface of dyad domains in the undivided cytoplasm after first meiotic division, and second meiotic division takes place simultaneously on both sides of the equatorial organelle band. Microtubules emanating from the telophase II nuclei interact to form columnar arrrays that interconnect all four nuclei, non-sister as well as sister. Cell plates are initiated in these columns of microtubules and expand centrifugally along the interface of opposing RMSs, coalescing in the center of the sporocyte and joining with the original sporocyte wall at the periphery to form the tetrad of microspores. Organelles are distributed into the spore domains in conjunction with RMSs. These data, demonstrating that cytokinesis in microsporogenesis can occur in the absence of both components of the typical cytokinetic apparatus (the preprophase band of microtubules which predicts the division site and the phragmoplast which controls cell-plate deposition), suggest that plant nuclei have an inherent ability to establish a domain of cytoplasm via radial microtubule systems and to regulate wall deposition independently of the more complex cytokinetic apparatus of vegetative cells.

Electronic Resource