Search Results - (Author, Cooperation:P. Wiesner)

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  1. 1
    Staff View
    Publication Date:
    2018-06-08
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Geosciences
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Immunology, Medicine, Diseases, Online Only
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    Publication Date:
    2013-05-31
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    ATP-Binding Cassette Transporters/deficiency/genetics/metabolism ; Animals ; Biological Transport ; Blood Vessels/embryology ; Carrier Proteins/genetics/*metabolism/secretion ; Cholesterol/analysis/*metabolism ; Embryo, Nonmammalian/blood supply/metabolism ; Endothelial Cells/metabolism ; Human Umbilical Vein Endothelial Cells ; Humans ; Lipoproteins, HDL/metabolism ; Membrane Lipids/metabolism ; Membrane Microdomains/chemistry/metabolism ; Neovascularization, Physiologic/*physiology ; Protein Multimerization ; Signal Transduction ; Vascular Endothelial Growth Factor Receptor-2/chemistry/metabolism ; Zebrafish/embryology/*metabolism ; Zebrafish Proteins/deficiency/genetics/*metabolism/secretion
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Staff View
    ISSN:
    1432-2307
    Keywords:
    Automated laser fluorescence sequencer ; Partial digestion ; Restriction enzyme fragment length analysis ; Asymmetrically labelled fluorescence primer ; Mycobacteria
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Medicine
    Notes:
    Abstract An automated method for the restriction fragment length polymorphism (RFLP) analysis for the differentiation of mycobacteria to the species level is described. After polymerase chain reaction (PCR) amplification of a sequence of the gene encoding the 65-kDa surface antigen common to all mycobacteria the product was investigated by RFLP analysis. For accurate determination of fragment sizes the asymmetrically fluorescein-labelled PCR product was partially digested with restriction site enzymes BstEII and HaeIII. The fragments obtained were analysed electrophoretically using an automated laser fluorescence DNA sequencer. Determination of fragment sizes revealed a deviation of ±1 base pair (bp; 0.6%) when compared to expected sizes. The validity of this approach was confirmed by analysing mycobacterial DNA obtained from pure cultures of Mycobacterium (M.) tuberculosis and alcohol-fixed smears as well as paraffin-embedded sputa of patients with culture-proven tuberculosis. Additionally a diagnostic algorithm was established by investigation of cultured M. bovis, M. bovis bacille Calmette-Guérin, M. avium, M. intracellulare and M. fortuitum. The method allows the identification of restriction enzyme sites which are only 40 bp apart. Partial restriction enzyme digestion of asymmetrically fluorescence-labelled PCR products will presumably lead to the discovery of new restriction enzyme sites.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00203742

    Articles: DFG German National Licenses