Search Results - (Author, Cooperation:P. W. Chee)

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  1. 1
    A. H. Paterson ; J. F. Wendel ; H. Gundlach ; H. Guo ; J. Jenkins ; D. Jin ; D. Llewellyn ; K. C. Showmaker ; S. Shu ; J. Udall ; M. J. Yoo ; R. Byers ; W. Chen ; A. Doron-Faigenboim ; M. V. Duke ; L. Gong ; J. Grimwood ; C. Grover ; K. Grupp ; G. Hu ; T. H. Lee ; J. Li ; L. Lin ; T. Liu ; B. S. Marler ; J. T. Page ; A. W. Roberts ; E. Romanel ; W. S. Sanders ; E. Szadkowski ; X. Tan ; H. Tang ; C. Xu ; J. Wang ; Z. Wang ; D. Zhang ; L. Zhang ; H. Ashrafi ; F. Bedon ; J. E. Bowers ; C. L. Brubaker ; P. W. Chee ; S. Das ; A. R. Gingle ; C. H. Haigler ; D. Harker ; L. V. Hoffmann ; R. Hovav ; D. C. Jones ; C. Lemke ; S. Mansoor ; M. ur Rahman ; L. N. Rainville ; A. Rambani ; U. K. Reddy ; J. K. Rong ; Y. Saranga ; B. E. Scheffler ; J. A. Scheffler ; D. M. Stelly ; B. A. Triplett ; A. Van Deynze ; M. F. Vaslin ; V. N. Waghmare ; S. A. Walford ; R. J. Wright ; E. A. Zaki ; T. Zhang ; E. S. Dennis ; K. F. Mayer ; D. G. Peterson ; D. S. Rokhsar ; X. Wang ; J. Schmutz
    Nature Publishing Group (NPG)
    Published 2012
    Staff View
    Publication Date:
    2012-12-22
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Alleles ; *Biological Evolution ; Cacao/genetics ; Chromosomes, Plant/genetics ; *Cotton Fiber ; Diploidy ; Gene Duplication/genetics ; Genes, Plant/genetics ; Genome, Plant/*genetics ; Gossypium/classification/*genetics ; Molecular Sequence Annotation ; Phylogeny ; *Polyploidy ; Vitis/genetics
    Published by:
    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    ISSN:
    1432-2242
    Keywords:
    Wheat ; Molecular ; Markers ; Breeding
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract The polymerase chain reaction (PCR) is an attractive technique for many genome mapping and characterization projects. One PCR approach which has been evaluated involves the use of randomly amplified polymorphic DNA (RAPD). An alternative to RAPDs is the sequence-tagged-site (STS) approach, whereby PCR primers are designed from mapped low-copy-number sequences. In this study, we sequenced and designed primers from 22 wheat RFLP clones in addition to testing 15 primer sets that had been previously used to amplify DNA sequences in the barley genome. Our results indicated that most of the primers amplified sequences that mapped to the expected chromosomes in wheat. Additionally, 9 of 16 primer sets tested revealed polymorphisms among 20 hexaploid wheat genotypes when PCR products were digested with restriction enzymes. These results suggest that the STS-based PCR analysis will be useful for generation of informative molecular markers in hexaploid wheat.
    Type of Medium:
    Electronic Resource
    URL:
    Articles: DFG German National Licenses