Search Results - (Author, Cooperation:P. Stephens)
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1Latest Papers from Table of Contents or Articles in PressI. Varela ; P. Tarpey ; K. Raine ; D. Huang ; C. K. Ong ; P. Stephens ; H. Davies ; D. Jones ; M. L. Lin ; J. Teague ; G. Bignell ; A. Butler ; J. Cho ; G. L. Dalgliesh ; D. Galappaththige ; C. Greenman ; C. Hardy ; M. Jia ; C. Latimer ; K. W. Lau ; J. Marshall ; S. McLaren ; A. Menzies ; L. Mudie ; L. Stebbings ; D. A. Largaespada ; L. F. Wessels ; S. Richard ; R. J. Kahnoski ; J. Anema ; D. A. Tuveson ; P. A. Perez-Mancera ; V. Mustonen ; A. Fischer ; D. J. Adams ; A. Rust ; W. Chan-on ; C. Subimerb ; K. Dykema ; K. Furge ; P. J. Campbell ; B. T. Teh ; M. R. Stratton ; P. A. Futreal
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-01-21Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Carcinoma, Renal Cell/*genetics ; Cell Line, Tumor ; Disease Models, Animal ; Gene Expression Regulation ; Gene Knockdown Techniques ; Humans ; Kidney Neoplasms/*genetics ; Mice ; Mutation/*genetics ; Nuclear Proteins/*genetics/*metabolism ; Pancreatic Neoplasms/genetics ; Transcription Factors/*genetics/*metabolismPublished by: -
2Latest Papers from Table of Contents or Articles in PressSchrock, A. B., Pavlick, D., Klempner, S. J., Chung, J. H., Forcier, B., Welsh, A., Young, L., Leyland-Jones, B., Bordoni, R., Carvajal, R. D., Chao, J., Kurzrock, R., Sicklick, J. K., Ross, J. S., Stephens, P. J., Devoe, C., Braiteh, F., Ali, S. M., Miller, V. A.
The American Association for Cancer Research (AACR)
Published 2018Staff ViewPublication Date: 2018-04-14Publisher: The American Association for Cancer Research (AACR)Print ISSN: 1078-0432Electronic ISSN: 1557-3265Topics: MedicinePublished by: -
3Latest Papers from Table of Contents or Articles in PressN. Tsolakos, T. N. Durrant, T. Chessa, S. M. Suire, D. Oxley, S. Kulkarni, J. Downward, O. Perisic, R. L. Williams, L. Stephens, P. T. Hawkins
National Academy of Sciences
Published 2018Staff ViewPublication Date: 2018-11-28Publisher: National Academy of SciencesPrint ISSN: 0027-8424Electronic ISSN: 1091-6490Topics: BiologyMedicineNatural Sciences in GeneralPublished by: -
4Articles: DFG German National LicensesStephens, P. J. ; Jalkanen, K. J.
College Park, Md. : American Institute of Physics (AIP)
Published 1989Staff ViewISSN: 1089-7690Source: AIP Digital ArchiveTopics: PhysicsChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesJalkanen, K. J. ; Stephens, P. J. ; Lazzeretti, P. ; Zanasi, R.
College Park, Md. : American Institute of Physics (AIP)
Published 1989Staff ViewISSN: 1089-7690Source: AIP Digital ArchiveTopics: PhysicsChemistry and PharmacologyNotes: It was recently shown that vibrational rotational strengths of chiral molecules can be expressed in terms of two nuclear shielding tensors, γλαβ(0) and ξˆλαβ(0). We report the first calculations of vibrational rotational strengths from these nuclear shielding tensors. The molecule studied is NHDT. Calculations are carried out at the SCF level using the random phase approximation. γλαβ(0) is calculated using length, momentum and force representations. ξˆλαβ(0) is calculated using angular momentum and torque representations. A range of basis sets, both "conventional'' and "polarized'' in type, are employed. Gauge and origin dependence are also investigated.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStephens, P. H. ; Cuello, A. C. ; Sofroniew, M. V. ; Pearson, R. C. A. ; Tagari, P
Oxford, UK : Blackwell Publishing Ltd
Published 1985Staff ViewISSN: 1471-4159Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Abstract: Acetyl-coenzyme A: choline O-acetyltransferase (EC 2.3.1.6) (ChAT) enzyme activity was measured in the nucleus basalis and other microscopically identified brain areas at various times after unilateral cortical lesions were made in the rat. Initially, a significant decrease in ChAT activity was detected in the nucleus basalis ipsilateral to the lesion. However, after 120 days ChAT activity had apparently recovered, as levels of the enzyme at that time were not significantly different from control values. No changes in ChAT activity could be detected in any of the other brain areas similarly studied. The significance of these findings and their relationship to the morphological changes seen in neurones of the nucleus basalis after cortical lesions are discussed.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesKoller, Daniel ; Martin, Michael C. ; Stephens, P. W. ; Mihaly, L.
[S.l.] : American Institute of Physics (AIP)
Published 1994Staff ViewISSN: 1089-7623Source: AIP Digital ArchiveTopics: PhysicsElectrical Engineering, Measurement and Control TechnologyNotes: A sample chamber has been designed to simultaneously measure the DC resistivity and IR transmission of C60 thin films while the films are doped with alkali metals in-situ. The chamber construction allows a choice of windows to cover the entire IR and visible range, while x-ray diffraction studies are also possible to determine the sample stoichiometry.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1460-9592Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: A retrospective analysis of the first 50 cases of neonatal cleft lip repair performed at the Hospital for Sick Children is presented. The patient population included 11 expremature infants of less than 45 weeks postconceptual age. There was no mortality at the time of follow-up. There was one case of peroperative hypoxaemia. There was one case of postoperative laryngospasm requiring reintubation. Postoperatively there were four cases of mild hypoxaemia and one patient with transient apnoea. No patients required blood transfusion. Seventy-six percent of patients did not require opioid analgesia. The remainder received a single dose of intramuscular codeine phosphate. The advantages and risks of anaesthesia for cleft lip in the neonatal period are reviewed. Recommendations for safe practice include the selection of gestationally mature infants with no intercurrent illness, avoidance of opioid analgesia, adequate staffing ratios of experienced postoperative nursing care, appropriate monitoring including oximetry and apnoea detectors.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1365-2044Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesThomas, D. W. ; Stephens, P. ; Stephens, M. ; Patten, D. W. ; Lim, S. H.
Oxford, UK : Blackwell Publishing Ltd
Published 1997Staff ViewISSN: 1600-0714Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: To determine whether the T-cell inflammatory infiltrate in oral lichen planus (OLP) represents a selective activation and expansion of a limited repertoire of T-cell receptor (TCR) specific T-cells. Vβ gene expression was investigated in lesional T-lymphocytes in OLP. A reverse transcriptase-polymerase chain reaction (RT-PCR) technique was used to amplify the 24 major Vβ gene sub-families of infiltrating mucosal lymphocytes and peripheral blood mononuclear cells (PMNC) in seven patients with reticular OLP and four healthy control patients. Specificity of amplified products was confirmed by Southern blotting with a Cp internal probe. TCR Vβ usage by lesional T-cells in OLP was markedly heterogeneous 5–23 Vβ sub-families). In 6/8 patients with OLP. Vβ usage was restricted with 〈inlineGraphic alt="leqslant R: less-than-or-eq, slant" extraInfo="nonStandardEntity" href="urn:x-wiley:09042512:JOP105:les" location="les.gif"/〉20/25 sub-families detected: only one of the Vβ sub-families (Vβ 8) was present in all of the OLP patients demonstrating TCR Vβ restriction. In contrast. TCR Vβ usage was unrestricted in PMNC from OLP patients and controls (〈inlineGraphic alt="leqslant R: less-than-or-eq, slant" extraInfo="nonStandardEntity" href="urn:x-wiley:09042512:JOP105:les" location="les.gif"/〉23/25 sub-families detected). In three patients, certain Vβ sub-families (Vβ13, Vβ14 & Vβ15) were present in the lesional T-cell population but were under-represented in PMNC. These results suggest a selective Vβ gene usage by lesional infiltrating T-cells in oral lichen planus. The non-uniformity of Vβ restriction in lesional T-cells does not support the concept of a common superantigen in OLP and reflects the heterogeneity of the disease.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStephens, P. ; Davies, K.J. ; Occleston, N. ; Pleass, R.D. ; Kon, C. ; Daniels, J. ; Khaw, P.T. ; Thomas, D.W.
Oxford, UK : Blackwell Science Ltd
Published 2001Staff ViewISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Background Oral mucosal wounds are characterized by rapid re-epithelialization and remodelling. In vitro, oral mucosal fibroblasts exhibit a fetal phenotype with increased extracellular matrix reorganizational ability, migration and experimental wound repopulation when compared with skin fibroblasts. Objectives To investigate whether phenotypic differences in the expression and production of matrix metalloproteinase (MMP) -2 and tissue inhibitors of metalloproteinases (TIMPs) could play an important part in mediating these in vitro differences. Methods Skin and oral mucosal fibroblast MMP-2, TIMP-1 and TIMP-2 mRNA expression and protein production were studied in three-dimensional collagen lattices using quantitative competitive reverse transcriptase–polymerase chain reaction (QCRT–PCR), enzyme-linked immunosorbent assay (ELISA), zymography and reverse zymography. Results Oral mucosal fibroblasts exhibited increased levels of the 62-kDa active form of MMP-2 and lattice contraction when compared with skin fibroblasts. Oral mucosal and skin fibroblast MMP-2 gene expression and synthesis of the 72-kDa pro-MMP-2 was similar as assessed by QCRT–PCR, zymography and ELISA. Differential MMP-2 activation was, however, related to phenotypic differences in TIMP activity between the skin and oral mucosal fibroblasts, as assessed by reverse zymography. Conclusions These studies propose a mechanism by which fibroblast phenotype may contribute directly to the observed preferential remodelling of oral wounds.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesMoseley, R. ; Stewart, J.E. ; Stephens, P. ; Waddington, R.J. ; Thomas, D.W.
Oxford, UK : Blackwell Science Ltd
Published 2004Staff ViewISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: The new era of pharmacogenetics has identified a potential for individuals to receive customized treatments for a variety of disease states. For such individualized treatments to fulfil their potential, it will be essential for clinicians to be able to monitor disease activity, ideally in a rapid, noninvasive fashion. The accessibility of the skin offers much potential to develop noninvasive tests of metabolic and disease activity for clinical use. Impaired human wound healing in the skin is a chronic inflammatory disorder in which the development of such tests has considerable potential, aiding clinical decision making and monitoring responses to treatment. This review article discusses how studies in other human diseases have highlighted potential biochemical markers (biomarkers) of disease activity in secreted biofluids, as aids to determining disease and metabolic activity within tissues. Using, as examples, lessons learned in the study of disease activity and prognosis of other chronic inflammatory conditions, such as osteoarthritis and periodontal disease, this review highlights the potential of dermal extracellular matrix (ECM) components (collagens, proteoglycans, hyaluronan and glycoproteins) for such uses. The limitations of currently utilized techniques and the concept that analysis of ECM components in wound fluid may represent useful biomarkers of disease activity are also discussed.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStephens, P. ; Wall, I.B. ; Wilson, M.J. ; Hill, K.E. ; Davies, C.E. ; Hill, C.M. ; Harding, K.G. ; Thomas, D.W.
Oxford, UK : Blackwell Science Ltd
Published 2003Staff ViewISSN: 1365-2133Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Summary Background Anaerobic cocci are estimated to be present in the deep tissues of over 50% of chronic skin wounds. While the part they play in the chronicity of these wounds is uninvestigated, anaerobic cocci have previously been shown to be involved in other chronic inflammatory human conditions. Methods In this study the anaerobic microflora of the deep tissues of 18 patients with refractory chronic venous leg ulcers (mean age 80·3 years; mean duration 〉 24 months) was characterized using strict anaerobic culture conditions. The effect of the anaerobic organisms isolated from these tissues on extracellular matrix (ECM) proteolysis and cellular wound healing responses was studied using in vitro models. Results Anaerobic organisms were present in the deep tissues of 14 of 18 wounds and were principally Peptostreptococcus spp. The effects of three Peptostreptococcus spp. isolated from these wounds (P. magnus, P. vaginalis and P. asaccharolyticus) on cellular wound healing responses were compared with those of two pathogenic organisms also isolated from these wounds (Pseudomonas aeruginosa and Citrobacter diversus). While the direct ECM proteolytic activity exhibited by the Peptostreptococcus spp. was limited, they did significantly inhibit both fibroblast and keratinocyte proliferation, but only at high concentrations. However, at lower concentrations peptostreptococcal supernatants profoundly inhibited keratinocyte wound repopulation and endothelial tubule formation. The magnitude of these effects varied between strains and they were distinct from those demonstrated by Pseudomonas aeruginosa and Citrobacter diversus. Conclusions These studies confirm the importance of anaerobic organisms in chronic wounds and demonstrate an indirect, strain-specific mechanism by which these microorganisms may play a part in mediating the chronicity of these wounds.Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesCHENG, J. C. ; OSBORNE, G. A. ; STEPHENS, P. J. ; EATON, W. A.
[s.l.] : Nature Publishing Group
Published 1973Staff ViewISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Most molecules of biological interest exhibit no electronic absorption, and hence no CD or MCD, in the rtear IR. However, in proteins containing transition-metal ions electronic absorption bands commonly occur in the near IR. The study of these bands is often useful in elucidating metal-ligand ...Type of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Fig. 1 Horizontal canal of Loligo vulgaris of 14 cm mantle length. Left side, seen from behind. The cupula is stained with diluted squid's ink. In all the figures the anticristae and hamuli have been given the numbers used by Dilly, Stephens and Young5. CTA and CTP are the anterior and posterior ...Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] This absorption band has since been used as a sensitive monitor of the oxygen content of silicon crystals pulled in this laboratory. The observed fall-off in oxygen content across a slice cut perpendicular to the direction of crystal growth shows that crystals pulled in a vacuum lose oxygen by ...Type of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesStaff View
ISSN: 0167-4781Keywords: (Rainbow trout) ; Cd stabilisation ; E. coli expression ; Enzyme purification ; Immunochemical characterization ; Metallothionein ; Recombinant proteinSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicinePhysicsType of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 0022-4731Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyType of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesStaff View
ISSN: 0009-2614Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: Chemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesNordvall, S. L. ; Janson, C. ; Kalm-Stephens, P. ; Foucard, T. ; Torén, K. ; Alving, K.
Oxford, UK : Munksgaard International Publishers
Published 2005Staff ViewISSN: 1398-9995Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Exhaled nitric oxide (NO) reflects inflammation in the lower airways and is well adapted for use in children. The aims of this study were to investigate the distribution of the fraction of expired NO (FENO) in school children and to compare FENO and spirometry in relation to the International Study of Asthma and Allergies in Childhood questionnaire. The study was performed in 959 randomly selected 13–14-year-old school children in Uppsala, Sweden. Exhaled NO was measured at an inhalation rate of 0.1 l/s (FENO0.1) and a spirometric test was performed and data from these measurements were related to questionnaire data. Exhaled NO was measured according to American Thoracic Society recommendations, except the use of a mouth wash and an exhalation flow rate of 0.1 l/s. The distribution of the mean FENO0.1 values was skewed, with a preponderance of very low levels and a widespread tail of values ranging up to 102 parts per billion (ppb). Boys exhibited significantly higher mean FENO0.1 values than girls, 5.2 (4.7–5.7) vs 4.4 (4.0–4.8) ppb (geometric mean and 95% CI), P 〈 0.01). Children who reported wheezing in the last year had higher FENO0.1 values than children that had not, 8.5 (7.1–10.2) vs 4.3 (4.0–4.6) ppb, P 〈 0.001). The same association was found to most symptoms indicating hay fever and eczema. In contrast to this, only weak or inconsistent associations were found between asthma and spirometric indices. Exhaled NO levels were found to be independently related to male gender, wheeze and rhinoconjuctivitis but not to current eczema. In conclusion, exhaled NO was closely associated with reported asthma and allergy symptoms whereas spirometric indices such as percent predicted forced expiratory volume in 1 s were not. As most asthma cases in a population are mild, the findings suggest that exhaled NO is a sensitive marker of asthma and allergy.Type of Medium: Electronic ResourceURL: