Search Results - (Author, Cooperation:P. J. Lehner)
-
1Latest Papers from Table of Contents or Articles in PressI. A. Tchasovnikarova ; R. T. Timms ; N. J. Matheson ; K. Wals ; R. Antrobus ; B. Gottgens ; G. Dougan ; M. A. Dawson ; P. J. Lehner
American Association for the Advancement of Science (AAAS)
Published 2015Staff ViewPublication Date: 2015-05-30Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Antigens, Neoplasm/genetics/*metabolism ; *Chromosomal Position Effects ; Conserved Sequence ; Drosophila melanogaster/genetics/metabolism ; Evolution, Molecular ; *Gene Silencing ; Genes, Reporter ; Genetic Loci ; Green Fluorescent Proteins/genetics ; HeLa Cells ; Heterochromatin/metabolism ; Histones/*metabolism ; Humans ; Immunoprecipitation ; Multiprotein Complexes/genetics/*metabolism ; Nuclear Proteins/genetics/*metabolism ; Phosphoproteins/genetics/*metabolism ; Protein Methyltransferases/metabolismPublished by: -
2Latest Papers from Table of Contents or Articles in PressM. P. Weekes ; S. Y. Tan ; E. Poole ; S. Talbot ; R. Antrobus ; D. L. Smith ; C. Montag ; S. P. Gygi ; J. H. Sinclair ; P. J. Lehner
American Association for the Advancement of Science (AAAS)
Published 2013Staff ViewPublication Date: 2013-04-13Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Antigens, CD34/analysis ; Cell Line, Tumor ; Cytomegalovirus/genetics/*physiology ; Cytomegalovirus Infections/*metabolism/*virology ; Dendritic Cells/physiology ; Down-Regulation ; Humans ; Lysosomes/metabolism ; Monocyte-Macrophage Precursor Cells/metabolism/virology ; Monocytes/metabolism/virology ; Multidrug Resistance-Associated Proteins/genetics/*metabolism ; Vincristine/metabolism/pharmacology ; Viral Proteins/*metabolism ; *Virus LatencyPublished by: -
3Articles: DFG German National LicensesStaff View
ISSN: 1432-1211Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract Major histocompatibility complex (MHC) class I molecules are heterodimers of a class I heavy chain and β2-microglobulin that bind peptides supplied by the MHC region-encoded transporters associated with antigen processing (TAP). Peptide binding by class I heterodimers is necessary for their maturation into stable complexes and is dependent on their physical association with TAP. In human mutant 721.220 cells, however, a novel genetic defect causes the failure of class I heterodimers to associate with TAP. This deficiency correlates with lack of expression of a glycoprotein, tapasin (TAP-associated glycoprotein), which has been found in association with class I heterodimers and TAP. Employing a transcomplementation analysis, we obtained evidence co-localizing the genetic defect of mutant 220 cells and the structural or a regulatory gene controlling the expression of tapasin on the short arm of chromosome 6, which includes the MHC. Expression of tapasin and the normal interaction of class I heterodimers with TAP are concomitantly restored, indicating the probable function of tapasin as a physical link between these complexes. In further support of this model, the absence of tapasin in mutant 220 cells correlates with reduced class I heterodimer stability, suggesting that tapasin may stabilize class I heterodimers and thereby enhance their association with TAP. These results further implicate tapasin in a mechanism that promotes peptide binding by class I heterodimers through their interaction with TAP.Type of Medium: Electronic ResourceURL: