Search Results - (Author, Cooperation:P. Deplazes)
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1Latest Papers from Table of Contents or Articles in PressI. J. Tsai ; M. Zarowiecki ; N. Holroyd ; A. Garciarrubio ; A. Sanchez-Flores ; K. L. Brooks ; A. Tracey ; R. J. Bobes ; G. Fragoso ; E. Sciutto ; M. Aslett ; H. Beasley ; H. M. Bennett ; J. Cai ; F. Camicia ; R. Clark ; M. Cucher ; N. De Silva ; T. A. Day ; P. Deplazes ; K. Estrada ; C. Fernandez ; P. W. Holland ; J. Hou ; S. Hu ; T. Huckvale ; S. S. Hung ; L. Kamenetzky ; J. A. Keane ; F. Kiss ; U. Koziol ; O. Lambert ; K. Liu ; X. Luo ; Y. Luo ; N. Macchiaroli ; S. Nichol ; J. Paps ; J. Parkinson ; N. Pouchkina-Stantcheva ; N. Riddiford ; M. Rosenzvit ; G. Salinas ; J. D. Wasmuth ; M. Zamanian ; Y. Zheng ; X. Cai ; X. Soberon ; P. D. Olson ; J. P. Laclette ; K. Brehm ; M. Berriman
Nature Publishing Group (NPG)
Published 2013Staff ViewPublication Date: 2013-03-15Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Adaptation, Physiological/*genetics ; Animals ; Biological Evolution ; Cestoda/drug effects/*genetics/physiology ; Cestode Infections/drug therapy/metabolism ; Conserved Sequence/genetics ; Echinococcus granulosus/genetics ; Echinococcus multilocularis/drug effects/genetics/metabolism ; Genes, Helminth/genetics ; Genes, Homeobox/genetics ; Genome, Helminth/*genetics ; HSP70 Heat-Shock Proteins/genetics ; Humans ; Hymenolepis/genetics ; Metabolic Networks and Pathways/genetics ; Molecular Targeted Therapy ; Parasites/drug effects/*genetics/physiology ; Proteome/genetics ; Stem Cells/cytology/metabolism ; Taenia solium/geneticsPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 0165-2427Keywords: [abr] Con A; Concanavalin A ; [abr] ELISA; enzyme-linked immunosorbent assay ; [abr] FCS; fetal calf serum ; [abr] HBSS; Hank's balanced salt solution ; [abr] PBS; phosphate-buffered saline ; [abr] PP; Peyer's patch ; [abr] SD; standard deviationSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: MedicineType of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesKock, N. P. ; Petersen, H. ; Fenner, T. ; Sobottka, I. ; Schmetz, C. ; Deplazes, P. ; Pieniazek, N. J. ; Albrecht, H. ; Schottelius, J.
Springer
Published 1997Staff ViewISSN: 1435-4373Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract In view of the increasing number of cases of human microsporidiosis, simple and rapid methods for clear identification of microsporidian parasites to the species level are required. In the present study, the polymerase chain reaction (PCR) was used for speciesspecific detection ofEncephalitozoon cuniculi, Encephalitozoon hellem, Encephalitozoon (Septata) intestinalis, andEnterocytozoon bieneusi in both tissue and stool. Using stool specimens and intestinal biopsies of patients infected withEnterocytozoon bieneusi (n=9),Encephalitozoon spp. (n=2), andEncephalitozoon intestinalis (n=1) as well as stool spiked with spores ofEncephalitozoon cuniculi andEncephalitozoon hellem and tissue cultures ofEncephalitozoon cuniculi andEncephalitozoon hellem, three procedures were developed to produce PCR-ready DNA directly from the samples. Specific detection of microsporidian pathogens was achieved in the first PCR. The subsequent nested PCR permitted species determination and verified the first PCR products. Without exception, the PCR assay confirmed electron microscopic detection ofEnterocytozoon bieneusi andEncephalitozoon intestinalis in stool specimens and their corresponding biopsies and in spiked stool samples and tissue cultures infected withEncephalitozoon cuniculi andEncephalitozoon hellem. Moreover, identification ofEncephalitozoon spp. could be specified asEncephalitozoon intestinalis. Whereas standard methods such as light and transmission electron microscopy may lack sensitivity or require more time and special equipment, the PCR procedure described facilitates speciesspecific identification of microsporidian parasites in stool, biopsies, and, probably, other samples in about five hours.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesKatzwinkel-Wladarsch, S. ; Deplazes, P. ; Weber, R. ; Löscher, T. ; Rinder, H.
Springer
Published 1997Staff ViewISSN: 1435-4373Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract The detection of microsporidial DNA by the polymerase chain reaction (PCR) has been suggested as an alternative or supplement to conventional microscopic methods. However, the relative merits of these techniques remain uncertain. In the present study, clinical specimens of different origin (stool, urine, sputum, nasal discharge, and cerebrospinal fluid) containing four different microsporidial species were blinded after microscopic examination and analyzed by PCR and subsequent restriction fragment length polymorphism (RFLP) to determine the respective species. Thirty-four specimens from 31 patients were evaluated, 16 of which were positive and 18 negative by microscopic examination; PCR detection of microsporidia produced identical results in 82% (28/34) of these specimens. Four samples were microscopically negative, PCR-positive, and two were microscopically positive, PCR-negative. Species determination by RFLP analysis of the amplified product was accurate for all isolates containingEnterocytozoon bieneusi, Encephalitozoon intestinalis, Encephalitozoon hellem, andEncephalitozoon cuniculi compared with microscopic identification of the genusEnterocytozoon or molecular analysis ofEncephalitozoon species after in vitro culture. Therefore, PCR-RFLP is useful for the rapid detection and differentiation of microsporidian spores in clinical specimens.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1432-1955Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract Nu/+ mice (ZU.ICR-strain) experimentally infected withGiardia lamblia (clone GS/M-83-H7) cleared the infection by day 45 postinfection (p.i.). Athymic nu/nu mice were reconstituted with immune Peyer's patch lymphocytes obtained from self-healed nu/+ littermates and thus acquired the potential to decrease their intestinal parasite mass. Intestinal B-cells from self-healed nu/+ mice as well as from immune-reconstituted athymic nude mice synthesized in vitro parasite-specific immunoglobulin A (IgA). This IgA was subsequently analyzed by immunoblotting, showing a predominant reaction with the major surface antigen (a 72000-Da polypeptide) characterizing theGiardia clone in question. The hypothesis on the causative role of intestinal IgA and immune lymphocytes in the control ofG. lamblia infection thus deserves further attention.Type of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1432-1955Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract Echinococcus multilocularis oncospheres, primary vesicular cysts, and protoscolices were assessed in vitro and in vivo for their potential to synthesize a PAS-positive laminated layer containing monoclonal antibody (mAb) G11-binding Em2 antigen. The presence of Em2 antigen in developed oncospheres and cysts was subsequently correlated to the potential of in vivo development into a secondary metacestode in recipient host mice, which also responded by anti-Em2 serum antibody formation. In contrast, protoscolices failed to develop the “Em2-positive” layer in vitro under the selected experimental conditions. The failure to develop subsequently in vivo into a secondary metacestode was underlined by a lack of anti-Em2 serum antibody formation by the hosts. We furthermore developed a technique to obtainE. multilocularis clones by inoculating single oncospheres into recipient mice.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesDeplazes, P. ; Gottstein, B. ; Eckert, J. ; Jenkins, D. J. ; Ewald, D. ; Jimenez-Palacios, S.
Springer
Published 1992Staff ViewISSN: 1432-1955Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract An enzyme-linked immunosorbent assay (ELISA) was developed for the detection ofEchinococcus coproantigens in fecal samples from dogs, dingoes or foxes infected with eitherE. granulosus orE. multilocularis. The ELISA was based on protein-A-purified polyclonal antibodies [anti-E. granulosus excretory/secretory (E/S) antigens]. The specificity of the assay as determined in 155 samples derived from carnivores that were free of helminth infection (n=37) or infected with non-Echinococcus cestodes (n=76) or with various nematodes (n=42) was found to be 98% overall. The diagnostic sensitivity was strongly dependent on the homologous worm burden. All 13 samples from foxes harboring 〉1,000E. multilocularis worms and 13 of 15 (87%) samples from dogs or dingoes containing 〉200E. granulosus worms were ELISA-positive, whereas 34 of 46 samples from foxes harboring 〈1,000E. multilocularis and 9 of 10 samples from dogs or dingoes bearing 〈200E. granulosus tested negative. Experimental prepatent infections of dogs withE. granulosus revealed positive ELISA reactions within the prepatent period (10–20 days post-infection) for six animals bearing 〉1,000E. granulosus each; a low worm burden (〈1,000 tapeworms/animal) resulted in ELISA positivity in only 2 of 3 animals at 30 days post-infection at the earliest. All five dogs that had been experimentally infected withE. multilocularis tested positive in the coproantigen ELISA as early as on day 5 post-infection.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1432-1955Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract Encephalitozoon cuniculi has a wide host range among mammals, but whether it represents a homogeneous species is a subject of controversy. We have isolated, cultivated (in human MRC-5 cells) and, for the first time, characterized by immunological and molecular biological methods four isolates of E. cuniculi from Norwegian blue foxes with a history of encephalitozoonosis. The isolates were compared with nine isolates from domestic rabbits from Switzerland. Two E. cuniculi subtypes were identified according to their host species. A 5′-GTTT-3′ tetranucleotide repeat was present twice in the rDNA intergenic spacer in all isolates from foxes as opposed to three times in all isolates from rabbits. Furthermore, random amplified polymorphic DNA analysis showed one polymorphic band among the subtypes, and Western-blot analysis using serum from an infected fox discriminated between the two subtypes on the basis of their banding patterns in the ranges of 31–33 and 38–40 kDa. The 5′-GTTT-3′ tetranucleotide repeat is a valuable genetic marker for these two subtypes of E. cuniculi and will be of use in continued studies on the molecular epidemiology of this parasite.Type of Medium: Electronic ResourceURL: -
9Latest Papers from Table of Contents or Articles in PressVogt, C. M., Armua-Fernandez, M. T., Tobler, K., Hilbe, M., Aguilar, C., Ackermann, M., Deplazes, P., Eichwald, C.
The American Society for Microbiology (ASM)
Published 2018Staff ViewPublication Date: 2018-02-21Publisher: The American Society for Microbiology (ASM)Print ISSN: 0019-9567Electronic ISSN: 1098-5522Topics: MedicinePublished by: