Search Results - (Author, Cooperation:P. C. Doherty)
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1Latest Papers from Table of Contents or Articles in PressR. A. Fouchier ; A. Garcia-Sastre ; Y. Kawaoka ; W. S. Barclay ; N. M. Bouvier ; I. H. Brown ; I. Capua ; H. Chen ; R. W. Compans ; R. B. Couch ; N. J. Cox ; P. C. Doherty ; R. O. Donis ; H. Feldmann ; Y. Guan ; J. M. Katz ; O. I. Kiselev ; H. D. Klenk ; G. Kobinger ; J. Liu ; X. Liu ; A. Lowen ; T. C. Mettenleiter ; A. D. Osterhaus ; P. Palese ; J. S. Peiris ; D. R. Perez ; J. A. Richt ; S. Schultz-Cherry ; J. Steel ; K. Subbarao ; D. E. Swayne ; T. Takimoto ; M. Tashiro ; J. K. Taubenberger ; P. G. Thomas ; R. A. Tripp ; T. M. Tumpey ; R. J. Webby ; R. G. Webster
American Association for the Advancement of Science (AAAS)
Published 2013Staff ViewPublication Date: 2013-01-25Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Biomedical Research/*trends ; Birds ; Humans ; *Influenza A Virus, H5N1 Subtype ; Influenza in Birds/*transmission/*virology ; Influenza, Human/*transmission/*virologyPublished by: -
2Latest Papers from Table of Contents or Articles in PressR. A. Fouchier ; A. Garcia-Sastre ; Y. Kawaoka ; W. S. Barclay ; N. M. Bouvier ; I. H. Brown ; I. Capua ; H. Chen ; R. W. Compans ; R. B. Couch ; N. J. Cox ; P. C. Doherty ; R. O. Donis ; H. Feldmann ; Y. Guan ; J. Katz ; H. D. Klenk ; G. Kobinger ; J. Liu ; X. Liu ; A. Lowen ; T. C. Mettenleiter ; A. D. Osterhaus ; P. Palese ; J. S. Peiris ; D. R. Perez ; J. A. Richt ; S. Schultz-Cherry ; J. Steel ; K. Subbarao ; D. E. Swayne ; T. Takimoto ; M. Tashiro ; J. K. Taubenberger ; P. G. Thomas ; R. A. Tripp ; T. M. Tumpey ; R. J. Webby ; R. G. Webster
American Association for the Advancement of Science (AAAS)
Published 2012Staff ViewPublication Date: 2012-01-28Publisher: American Association for the Advancement of Science (AAAS)Print ISSN: 0036-8075Electronic ISSN: 1095-9203Topics: BiologyChemistry and PharmacologyComputer ScienceMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; *Biomedical Research ; Disease Models, Animal ; Ferrets ; Humans ; *Influenza A Virus, H5N1 Subtype/pathogenicity ; Influenza, Human/transmission/virology ; Orthomyxoviridae Infections/*transmission/virologyPublished by: -
3Articles: DFG German National LicensesStaff View
ISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesDoherty, P. C. ; Korngold, R. ; Schwartz, D. H. ; Bennik, J. R.
Oxford, UK : Blackwell Publishing Ltd
Published 1981Staff ViewISSN: 1600-065XSource: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesDoherty, P. C. ; Blanden, R. V. ; Zinkernagel, R. M.
Oxford, UK : Blackwell Publishing Ltd
Published 1976Staff ViewISSN: 1600-065XSource: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 1600-065XSource: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineType of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: A severe inflammatory process can be induced by transferring lymphocytic choriomeningitis virus (LCMV)-immune spleen cell populations into cyclophosphamide (Cy)-suppressed, LCMV-infected recipients. The extent of cellular invasion can be quantified accurately by counting cells obtained from the cisterna magna. The underlying lymphocyte-target cell interactions are apparently very specific, with massive cellular invasion being dependent on the donor and recipient sharing class I major histocompatibility complex glycoproteins. Unlike the situation found by others for delayed-type hypersensitivity in the mouse footpad, the inflammatory process in the cisterna magna was induced to a similar extent by unmanipulated LCMV-immune spleen cells and by lymphocyte populations from mice that were pretreated with 150–200 mg/kg of Cy. This dose of Cy did not eliminate cytotoxic T lymphocytes (CTL) in the mouse strains used. However, immune spleen cells from Cy-pretreated mice showed a reduced capacity to clear virus from the brain. Virus persisted even though the inflammatory process resolved. These results support earlier conclusions that immune CTL are directly responsible for the induction of severe meningitis in LCM, although CTL obviously do not constitute the sole effectors involved in eliminating virus from the central nervous system.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Mice injected intracerebrally with a sublethal dose of vaccinia virus develop severe meningitis. The number of inflammatory cells in cerebrospinal fluid (CSF) increases approximately threefold each day for 3 to 7 days after intracerebral challenge, subsequent to which samples can no longer be obtained because the cisterna magna is obliterated owing to brain swelling. Examination of this inflammatory exudate during the later stages of this pathological process shows evidence of both cytotoxic T lymphocyte (CTL) function and the presence of Lyt1+ and Ly12+ cells on days 6 and 7, In addition, potent non-T-cell (Lyt2−) cytotoxic activity is found in CSF taken from younger (12 weeks) mice as early as after day 3 and is still present on day 6. The level of non-T-cell-mediated cytotoxicity in CSF on day 5 or 6 (but not day 3) is considerably decreased in animals that were also given a large dose of virus intravenously to maximize T-cell stimulation in lymphoid tissue, and the extent of CTL activity is concomitantly increased. The diminution of non-specific cytotoxic function does not seem to reflect simple dilution in the presence of excess virus-immune T cells.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] THE nature of surface changes induced, by interactions between viruses and cell membranes is currently a topic of considerable interest to many virologists and cellular immunologists. This follows the realisation that cell-mediated immunity in virus diseases reflects surface monitoring of virally ...Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] Both the cytotoxic assay used and the characteristics of the cells involved have been described previously6"8. Briefly, monolayers of C3H mouse fibroblasts (L cells) are grown in plastic tissue culture trays, infected with a high multiplicity of the WE3 strain of LCM virus and the cells labelled ...Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesShimoda, Kazuya ; van Deursent, Jan ; Sangster, Mark Y. ; Sarawar, Sally R. ; Carson, Richard T. ; Tripp, Ralph A. ; Chu, Charles ; Quelle, Frederick W. ; Nosaka, Tetsuya ; Vignali, Dario A. A. ; Doherty, P. C. ; Grosveld, G. ; Paul, W. E. ; Ihle, J. N.
[s.l.] : Nature Publishing Group
Published 1996Staff ViewISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] We disrupted the murine Statd gene by replacing the first coding exon with a neo resistance gene cassette (Fig. la). Appropriately targeted embryonic stem (ES) cell clones were used to obtain chimaeric mice that transmitted the disrupted locus through the germ line. Breeding ofStat6+/ mice ...Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: The inflammatory exudate found in cerebrospinal fluid (CSF) of mice 6 days after intracerebral infection with lymphocytic choriomeningitis virus (LCMV) contains substantial populations of both cytotoxic T lymphocytes (CTL) and natural killer (NK) cells. Removal of NK cell activity by in vivo treatment with antibody to the asialo GM, ganglioside and studies with NK-deficient bg/bg mice did not clearly determine whether NK cells contribute in any way to the development of clinical LCM. However, the LCM disease process induced in cyclophosphamide-suppressed, LCMV-infected recipients by the adoptive transfer of LCMV-immune spleen cells occurs in the absence of NK cell effector function in spleen, lymph nodes, or CSF of the recipients, though potent CTL populations are present in all of these sites. In this situation, NK cells are apparently not required for the induction of neurological symptoms that are indistinguishable from those of classical LCM.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Cytotoxic T lymphocytes (CTL) are able to eliminate P815 (DBA/2) mastocytoma cells growing in cerebrospinal fluid of BALB/c H-2-compatible but minor histocompatibility (H) antigen-different mice and in H-2-incompatible C3H/He mice. We examined the magnitude of the primary CTL response to multiple, minor H antigens and to determinants of the major histocompatibility complex (MHC) by using a direct cytolytic assay and limiting-dilution analysis to estimate CTL frequency. By these criteria, no obvious differences emerged, and the responses appeared comparable at the site of inflammatory process, despite differences in the number of clonal progenitors. Experiments with radiation chimeras showed evidence of a strong cytotoxic T-cell response against P815 cells in [(ddd × bbb)F1→ ddd] and (F1→ bbd), but not in (F1→ bbb) radiation chimeras. Therefore, this cytotoxic T-cell response against minor H antigens obeys the postulated rules for thymic restriction of precursors. Compatibility at the H-2 D-end of the MHC is apparently sufficient to ensure a strong response.Type of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Cell-mediated lysis of target monolayers intccted with lymphocytic choriomeningitis virus has previously been shown to be a property of specifically sensitized thymus-derived lymphocytes (T cells). The cytotoxic potential of individual effector lymphocytes is maximally expressed when infected monolayers are confluent and overlaying immune spleen cells cover less than the total target area. The data presented support the following interpretations [l] Cell-mediated injury depends on close association between sensitized T cells and infected L cells. [2] Any attachment made is readily reversible. [3] Effector lymphocytes may damage more than one target cell, operating, perhaps, in a rather circumscribed area. [4] Cell death is a ‘one hit’ phenomenon. [5] Cytolysis is a property of individual T cells, there being no obvious requirement for co-operation between different populations of spleen cells, [6] Substances secreted into the medium at large are neither directly cytotoxic nor capable of sensitizing normal spleen cells. [7] Specific lysis occurs at temperatures ranging from 27°C to 40°C, being maximal at the higher temperatureType of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 1365-3083Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Administration of a large dose (400 mg/kg) of Cyclophosphamide (Cy) prior to infection with lymphocytic choriomeningitis virus (LCMV) suppresses the virus-immune cytotoxic T lymphocyte (CTL) response. Treatment with 150 mg/kg of Cy before, at the time of, or on the day after LCMV infection has little effect on CTL function. In contrast, when given on 2-7 days after LCMV, this dose profoundly depresses the day-8 CTL response and delays the onset of neurological disease. Administration of as little as 50 mg/kg of Cy has a marked effect on CTL activity when given 5 days after virus. Therefore it seems likely that CTL are replicating for 5 or 6 days during this primary response. Multiplication of these T cells is apparently completed by day 8, since inoculation of 150 mg/kg of Cy at this time has little effect on the level of CTL activity measured on day 9. The CTL activity is not reconstituted by in vitro culture with added helper factors. The interpretation that CTL are replicating in vivo following inoculation of LCMV is in accord with other analyses of virus-specific precursor frequency in primed and naive mice.Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 1420-9071Keywords: T cells ; major histocompatibility complex ; cell-mediated immunity ; immune response genes ; influenzaSource: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
17Articles: DFG German National LicensesBlanden, R. V. ; Dunlop, M. B. C. ; Doherty, P. C. ; Kohn, H. I. ; McKenzie, I. F. C.
Springer
Published 1976Staff ViewISSN: 1432-1211Source: Springer Online Journal Archives 1860-2000Topics: BiologyMedicineNotes: Abstract Lysis of ectromelia- or LCM virus-infected macrophage target cells by virus-specific cytotoxic T cells from mice immunized with the homologous virus occurred only where donors of T cells and target cells shared eitherH-2K orH-2D genes. With both viruses, use of T cell or target cell donors bearing mutations (B6.C-H-2ba, B6-H-2bh, B6-H-2bg1, and B6-H-2bg2), all of which apparently occurred in the same single genetic element in theH-2Kb region, abolished (H-2ba) or impaired (H-2bh,H-2bg1 andH-2bg2) lysis in T cell-target cell combinations that shared (apart from the mutations) all other genes in theK, I-A, orI-B regions of theH-2 complex. The data suggest that virus-induced antigenic patterns on infected B6.C-H- 2ba (mutant) cells are more different antigenically from those on C57BL/6 (wild type) cells than are those on infected cells from the other mutants -B6-H-2bh, B6-H-2bg1, and B6-H-2bg2. (B6.C-H-2ba× B6 -H-2bh)F1 mice behaved like B6-H-2bh, indicating no complementation, and confirming that theH-2K gene(s) involved in recognition of virus-infected cells by virus-specific T cells behave as a single element. These findings are discussed in relation to the nature of virus-induced antigenic patterns that are recognized by virus-specific cytotoxic T cells.Type of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 1432-1831Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Conclusions At this stage, the only cell population that is directly implicated in the induction of fatal lymphocytic choriomeningitis is the class I MHC-restricted immune T cell. However, it is not clear that cell-mediated lysis is central to the development of neurological symptoms or that the T cells act alone to cause immunopathology.Type of Medium: Electronic ResourceURL: