Search Results - (Author, Cooperation:P. Berg)

2018-03-06

The American Society for Pharmacology and Experimental Therapeutics (ASPET)

0026-895X

1521-0111

Chemistry and Pharmacology

Medicine

2018-02-17

Wiley-Blackwell

2045-7758

Biology

2018-03-28

The American Society for Microbiology (ASM)

0066-4804

1098-6596

Biology

Medicine

2018-02-08

Institute of Physics (IOP)

1757-8981

1757-899X

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

2014-05-09

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Arctic Regions ; *Biodiversity ; Carbon/metabolism ; *Carbon Cycle ; *Ecosystem ; Nitrogen/metabolism ; Nitrogen Cycle ; Plants/metabolism ; Tropical Climate

2018-02-08

Institute of Physics (IOP)

1757-8981

1757-899X

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

2015-03-21

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Biotechnology/ethics ; *Caspase 9 ; *Clustered Regularly Interspaced Short Palindromic Repeats ; Gene Transfer, Horizontal ; Genetic Engineering/*ethics ; Genetic Predisposition to Disease/*prevention & control ; Genome, Human/genetics ; Genomics ; *Germ Cells ; Humans ; Risk Management ; Targeted Gene Repair/*ethics

2012-09-18

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

*Accident Prevention ; Animals ; Biohazard Release/*prevention & control ; Biomedical Research/*ethics ; Birds ; *Containment of Biohazards ; DNA, Recombinant/*genetics ; Guidelines as Topic ; Humans ; Influenza A Virus, H5N1 Subtype/genetics/pathogenicity ; Influenza in Birds/prevention & control/transmission ; Influenza, Human/prevention & control/transmission ; Information Dissemination ; Organisms, Genetically Modified/*genetics ; Policy ; Publications ; United States ; United States Government Agencies

1432-0878

Myocardium, mitochondria, calcium ; Cryo-ultramicrotomy-X-ray microanalysis ; Ultrahistochemistry ; Cardiac surgery

Springer Online Journal Archives 1860-2000

Biology

Medicine

Summary Mitochondria of normal myocardial cells of the sand rat and the mouse as well as of the left ventricle of man, have been examined for their content of calcium. Ultrahistochemistry and X-ray microanalysis revealed two basically different inclusions: Osmiophilic mitochondrial granules and Spherical mitochondrial particles. Osmiophilic mitochondrial granules were found in conventionally fixed and plastic embedded tissues as well as in cryosections of chemically fixed and sucrose infused tissues. Such granules lacked inert electron density and probably consisted mainly of unsaturated lipids. X-ray spectra obtained from these tissues revealed no peaks for calcium. Spherical mitochondrial particles were present in dry-cut cryo-sections of N2-frozen tissues not treated by fixatives and/or cryoprotectants. These particles were deeply electron dense in unstained, freeze-dried cryo-sections. They usually measured from 600Å–900Å in diameter in the normal myocardium of the sand rat and the mouse and from 250 Å–400Å in diameter in the left ventricular myocardium of man. Significant calcium peaks could be identified in the X-ray spectra of these particles, whereas none occurred in the analyses of other tissue regions. Potassium was detected with about equal frequency in the particles and in other parts of the tissue. On the basis of the inert electron density of the particles and their absence in chemically fixed tissues as well as of the results of the X-ray analysis, it is concluded that they contain precipitates of extremely labile ions of mitochondrial calcium.

Electronic Resource

1432-0878

Cardiac ultrastructure ; Sarcoplasmic reticulum ; Myocardial calcium localization ; EDTA/EGTA ; X-ray microanalysis

Springer Online Journal Archives 1860-2000

Biology

Medicine

Summary The intracellular localization of calcium as an antimonate precipitate is studied in myocardial cells of a non-mammalian vertebrate. Pigeon papillary muscles are pretreated in a calcium-free potassium solution containing 60 mM KSb (OH)6, and fixed in 1 % OsO1 containing the same concentration of antimonate. Calcium is chelated by K-EDTA or K-BGTA, in part separating it from the sodium-calcium antimonate. Atomic absorption spectrophotometry is employed to study the precipitate formation when sodium and calcium ions are added to a pH controlled antimonate solution. The chelating effect of K-EDTA and K-EGTA on the precipitates is studied by the same method. Both sodium and calcium cations are heavily precipitated by the antimonate anion. More calcium ions are precipitated when sodium ions are also present in the solution. K-EDTA and K-EGTA do not redissolve more than about 50% of the calcium antimonate from a sodium-calcium antimonate precipitate. When calcium cations only are added to the antimonate solution, K-EGTA redissolves about 95% of the calcium antimonate precipitate. A direct evidence for the presence of calcium in the tissue precipitates is given by X-ray microanalyses of 2500 Å thick sections. Calcium antimonate is located to the sarcoplasmic reticulum (SR), the myofibrils, the mitochondria and the nuclei. In the SR, calcium antimonate is consistently found in the subsarcolemmal cisternae of the peripheral couplings (Sommer and Johnson, 1969) and in the Z line cisternae (Saetersdal and Myklebust, 1975) or extended junctional SR (Sommer and Johnson, 1970). Along the myofibrils, calcium antimonate is found at the overlap of thick and thin filaments. In sarcomeres with short I bands, a dense antimonate precipitate consisting of large granules is found at the A-I junction. No calcium is found at the Z lines. The calcium antimonate granules along the myofilaments seem to be related to the length of the sarcomeres. The significance of these findings is discussed in relation to functional aspects of the myocardial cell

Electronic Resource

1089-7690

AIP Digital Archive

Physics

Chemistry and Pharmacology

Electronic Resource

1089-7690

AIP Digital Archive

Physics

Chemistry and Pharmacology

The effect of H2 translational, rotational, and vibrational excitation on the dynamics of the O(1D2)/H2 reaction are explored in a semiclassical trajectory study involving both of the energetically accessible potential energy surfaces of the system. Landau–Zener probabilities determine surface hopping. At low reagent excitation, the deep H2O potential minimum dominates the dynamics, causing the reagents to reorient towards a H–O–H (insertion) configuration and form the H2O intermediate, irrespective of the initial approach geometry. High vibrational excitation enhances the probability for transitions onto the excited state potential during the interaction. Reactions which sample the excited state potential have fundamentally different dynamics from those which remain on the lower state. For reactions involving H2(v=4), the OH product has a bimodal vibrational distribution, peaking in OH(v'=2) and OH(v'=9). The lower peak is due to reactions which access the excited state potential; the higher peak results from those which remain on the lower state for the entire interaction. High translational excitation shortens the interaction time and reduces the effect of the potential minimum to reorient the reagents. Rotational excitation also reduces the effect of the potential minimum by causing the system to rotate out of the insertion geometry before entering the potential minimum.

Electronic Resource

1471-0528

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Summary. The efficacy of electronic fetal monitoring combined with fetal blood analysis during labour in identifying fetal distress was investigated in a retrospective study. Operative delivery for fetal distress diagnosed during labour was performed in 9% of 2659 deliveries. All had continuous fetal heart rate monitoring and 22% had a fetal scalp blood analysis. Operative delivery had been performed in 53% of the infants who were acidotic at birth (umbilical artery pH 〈7.20) and in 46% of those with a low modified Apgar score (〈7). These results show that the use of continuous fetal heart rate monitoring and fetal scalp blood sampling detects fetal distress without resulting in a high rate of operative delivery.

Electronic Resource

1089-7623

AIP Digital Archive

Physics

Electrical Engineering, Measurement and Control Technology

We report the development of a data acquisition system which can be used with any commercial Michelson interferometer to carry out time-resolved Fourier transform spectroscopy with a time resolution of one microsecond. The sensitivity and spectral resolution of the experiment are limited only by the interferometer. The instrument occupies one slot on a conventional VME enclosure (6U height). It includes all hardware and firmware to control the experiment which generates the spectral transients and to collect the time-resolved interferograms. We are presently transferring this design to a single 6U height printed circuit board (with a mezzanine), in order to make it available as a standalone system for use with any VME platform.

Electronic Resource

1469-8986

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Psychology

The topography of the postimperative negative variation (PINV) was analyzed in participants with high and low scores on the German version of the Schizotypal Personality Questionnaire. Scalp amplitude and Laplacian maps of the terminal contingent negative variation (tCNV) and PINV and the time course of the PINV were compared between the two groups. CNV and PINV were induced with a delayed matching-to-sample task, in which the pattern of the imperative stimulus was either clearly or ambiguous matched to one of the two diamonds simultaneously presented as a warning stimulus 4.0 s earlier. Electroencephalograms were recorded with a DC amplifier (32 channels). Negativity increased from tCNV to PINV, especially at frontal sites, and the PINV was larger under ambiguous than under clear matching conditions. Low-scoring participants showed a right-sided predominance of the PINV, which was absent in high-scoring participants. These results resemble differences in the topography of the PINV between healthy control participants and those with schizophrenia under identical experimental conditions and suggest functional differences between tCNV and PINV.

Electronic Resource

1469-8986

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Psychology

The aim of the present study was the comparative assessment of the 4-week test-retest stabilities of the saccadic CNV (sCNV) and saccadic reaction times (SRT) during the execution of pro- and antisaccades, as well as the stability of RT during execution of two neuropsychological tests of alertness and S-R incompatibility. Prosaccades were elicited under the 200-ms gap and overlap conditions, antisaccades under the overlap condition (64 trials each). The EEG was recorded from 25 channels with a DC amplifier (MES, Munich). Data of 20 healthy participants were statistically analyzed. We found high test-retest correlations for all SRT (.76 ≤rtt≤ .88) and neuropsychological (.62 ≤rtt≤ .88) measures. For the sCNV, coefficients ranging between .58 (pro/gap) and .77 (anti/overlap) were obtained. Whereas SRT were significantly faster during the second than during the first session, group means for the saccadic CNV were stable across the sessions. Our results suggest high 4-week stability of individual differences in SRT, and moderate to good stabilities of saccadic CNV amplitudes. Our results recommend these “traitlike” measures to be used in individual differences research.

Electronic Resource

1469-8986

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Psychology

Event-related potentials (ERPs) recorded from the human scalp can provide important information about how the human brain normally processes information and about how this processing may go awry in neurological or psychiatric disorders. Scientists using or studying ERPs must strive to overcome the many technical problems that can occur in the recording and analysis of these potentials. The methods and the results of these ERP studies must be published in a way that allows other scientists to understand exactly what was done so that they can, if necessary, replicate the experiments. The data must then be analyzed and presented in a way that allows different studies to be compared readily. This paper presents guidelines for recording ERPs and criteria for publishing the results.

Electronic Resource

1745-4557

Blackwell Publishing Journal Backfiles 1879-2005

Process Engineering, Biotechnology, Nutrition Technology

The role of game meats as a source of food has been largely ignored in modern times, but may provide the principle food source for many individuals. Twenty-two antelope were completely boned to obtain lean meat. The skinned and dressed cold carcasses yielded an average of 68.4% boneless meat tissue. The average moisture, protein and fat content was 73.9, 22.5 and 0.9%, respectively. Energy value and cholesterol content was 144.1 kcal/100 g and 121.5 mg/100 g, respectively. Antelope meat contained high levels of essential amino acids and was comparable to lean beef in amino acid content. Selected minerals in the longissimus muscle from the 22 animals revealed large amounts (mg/g) of K, Na, P and Mg and smaller amounts (μg/g) of Ca, Cu, Fe, Mn and Zn.

Electronic Resource

1745-4557

Blackwell Publishing Journal Backfiles 1879-2005

Process Engineering, Biotechnology, Nutrition Technology

The role of game meats as a food source has been largely ignored today, but may provide the principle meat source for many individuals. Fifteen whitetail deer were completely boned to obtain lean meat. A typical skinned and dressed cold carcass yielded 72% boneless lean tissue. The average moisture, protein and fat content were 73.5, 23.6 and 1.4%, respectively. Energy value and cholesterol content was 149 kcal/100g and 116 mg/100g, respectively. Venison contained high levels of essential amino acids and was comparable to lean beef in amino acid content. Selected minerals in the longissimus muscle from 13 animals revealed large amounts (mg/g) of K, Na and P and smaller amounts (μg/g) of Ca, Cu, Fe, Mg, Mn and Zn.

Electronic Resource

1089-7690

AIP Digital Archive

Physics

Chemistry and Pharmacology

We use fast time-resolved Fourier transform spectroscopy and low pressure infrared chemiluminescence techniques to determine the product energy distribution in the title reaction. We create the reagent hydrogen atoms with 2.3 eV of translational excitation by photofragmentation of H2S at 193 nm, and observe the time evolution of the infrared chemiluminescence from the product HCl(v',J') under single gas kinetic collision conditions. The initial vibrational distribution, determined from the first observation after creation of the H atoms is P(v'=1:2:3:4:5:6:7:8)=1.0:0.84:0.74:0.59:0.34:0.24:0.17:0.13. The initial HCl rotational distribution in each vibrational level is broad, showing no identifiable maximum. The fraction of the total available energy entering HCl vibration and rotation, respectively, are 0.19 and 0.10. The time evolution of the observed vibrational and rotational distributions gives information about the changes in the reaction dynamics consequent on reduction of the reagent translational energy.

Electronic Resource