Search Results - (Author, Cooperation:P. Alves)

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  1. 1
    Staff View
    Publication Date:
    2018-01-10
    Publisher:
    Wiley-Blackwell
    Print ISSN:
    0007-0963
    Electronic ISSN:
    1365-2133
    Topics:
    Medicine
    Published by:
    http://www.wiley.com/wiley-blackwell

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Staff View
    Publication Date:
    2018-02-01
    Publisher:
    Nature Publishing Group (NPG)
    Electronic ISSN:
    2045-2322
    Topics:
    Natural Sciences in General
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  3. 3
    Rodrigues, P. M., Ribeiro, A. R., Serafini, N., Meireles, C., Di Santo, J. P., Alves, N. L.
    The American Association of Immunologists (AAI)
    Published 2018
    Staff View
    Publication Date:
    2018-02-10
    Publisher:
    The American Association of Immunologists (AAI)
    Print ISSN:
    0022-1767
    Electronic ISSN:
    1550-6606
    Topics:
    Medicine
    Published by:
    http://www.aai.org/

    Latest Papers from Table of Contents or Articles in Press
  4. 4
    Staff View
    Publication Date:
    2012-09-08
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Alleles ; Cell Line ; DNA/*genetics ; *Encyclopedias as Topic ; GATA1 Transcription Factor/metabolism ; Gene Expression Profiling ; Gene Regulatory Networks/*genetics ; Genome, Human/*genetics ; Genomics ; Humans ; K562 Cells ; *Molecular Sequence Annotation ; Organ Specificity ; Phosphorylation/genetics ; Polymorphism, Single Nucleotide/genetics ; Protein Interaction Maps ; RNA, Untranslated/genetics/metabolism ; Regulatory Sequences, Nucleic Acid/*genetics ; Selection, Genetic/genetics ; Transcription Factors/*metabolism ; Transcription Initiation Site
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  5. 5
    Staff View
    Publication Date:
    2018-02-07
    Publisher:
    American Physical Society (APS)
    Print ISSN:
    0556-2821
    Electronic ISSN:
    1089-4918
    Topics:
    Physics
    Keywords:
    General relativity, alternative theories of gravity
    Published by:
    http://www.aps.org/

    Latest Papers from Table of Contents or Articles in Press
  6. 6
    E. P. Alves, J. Zrake, and F. Fiuza
    American Physical Society (APS)
    Published 2018
    Staff View
    Publication Date:
    2018-12-15
    Publisher:
    American Physical Society (APS)
    Print ISSN:
    0031-9007
    Electronic ISSN:
    1079-7114
    Topics:
    Physics
    Keywords:
    Plasma and Beam Physics
    Published by:
    http://www.aps.org/

    Latest Papers from Table of Contents or Articles in Press
  7. 7
    Staff View
    Publication Date:
    2018-03-06
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Geosciences
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Evolution
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  8. 8
    Staff View
    Publication Date:
    2018-03-09
    Publisher:
    Wiley-Blackwell
    Print ISSN:
    0048-6604
    Electronic ISSN:
    1944-799X
    Topics:
    Geosciences
    Physics
    Published by:
    http://www.wiley.com/wiley-blackwell

    Latest Papers from Table of Contents or Articles in Press
  9. 9
    Staff View
    Publication Date:
    2018-06-22
    Publisher:
    American Association for the Advancement of Science (AAAS)
    Print ISSN:
    0036-8075
    Electronic ISSN:
    1095-9203
    Topics:
    Biology
    Chemistry and Pharmacology
    Geosciences
    Computer Science
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Evolution
    Published by:
    http://www.aaas.org/

    Latest Papers from Table of Contents or Articles in Press
  10. 10
    Staff View
    Publication Date:
    2018-02-24
    Publisher:
    Wiley-Blackwell
    Print ISSN:
    0048-6604
    Electronic ISSN:
    1944-799X
    Topics:
    Geosciences
    Physics
    Published by:
    http://www.wiley.com/wiley-blackwell

    Latest Papers from Table of Contents or Articles in Press
  11. 11
    MOREIRA, J. L. ; ALVES, P. M. ; RODRIGUES, J. M. ; CRUZ, P. E. ; AUNINS, J. G. ; CARRONDO, M. J. T.

    Oxford, UK : Blackwell Publishing Ltd
    Published 1994
    Staff View
    ISSN:
    1749-6632
    Source:
    Blackwell Publishing Journal Backfiles 1879-2005
    Topics:
    Natural Sciences in General
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1111/j.1749-6632.1994.tb44368.x

    Articles: DFG German National Licenses
  12. 12
    Gilly, L.J. ; Henriet, P. ; Alves, P. ; Capron, P.C.

    Amsterdam : Elsevier
    Staff View
    ISSN:
    0029-5582
    Keywords:
    Br^8^1(α, n), (α, α n), σ(E) = 10-24 MeV ; NUCLEAR REACTIONS Br^7^9(α, n), (α, α n), (α, 2n) ; measured σ(E) Natural target
    Source:
    Elsevier Journal Backfiles on ScienceDirect 1907 - 2002
    Topics:
    Physics
    Type of Medium:
    Electronic Resource
    URL:
    http://linkinghub.elsevier.com/retrieve/pii/0029-5582(64)90160-9

    Articles: DFG German National Licenses
  13. 13
    Staff View
    ISSN:
    1432-0614
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    Abstract. The growth and productivity of an Sp2/0 cell line, F3b10, expressing a recombinant antibody (rAb) and BHK21 cells expressing either the same rAb from the same plasmids (BHK.IgG) or secreted alkaline phosphatase (SEAP) (BHK.SEAP) were investigated. The F3b10 line was grown as a single cell suspension. The BHK lines were grown either as suspended natural aggregates or on Cytodex 3 microcarriers. The data for F3b10 showed that the cell-specific rAb production rate (QsrAb) increased in parallel with increases in the specific growth rate (μ). A similar result was obtained for suspended aggregate cultures of both recombinant BHK cell lines. In contrast, for microcarrier cultures of both BHK cell lines, Qsproduct increased as μ decreased. This report shows that the relationship between cell growth and Qsproduct for the cell lines and products studied is dependent upon the culture process. In systems where recombinant cells are growing as a single cell suspension or within a natural suspension aggregrate, Qsproduct increased with increases in μ. In such systems, the cells have a rounded morphology. When cells were grown on microcarriers, Qsproduct decreased as μ increased. Cells growing attached to a surface are flat and elongated. The observed differences in the relationship of Qsproduct to μ are correlated with changes in cell morphology. The relationship between Qsproduct and μ is also affected by the choice of cell line.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s002530050079

    Articles: DFG German National Licenses
  14. 14
    Staff View
    ISSN:
    1432-0614
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    Abstract Some anchorage-dependent animal cells can form natural aggregates in stirred tanks. Baby hamster kidney (BHK) natural aggregates are described and characterized. Total cell concentration and viability could be obtained after aggregate mechanical dissociation, with negligible cell lysis and no change in cell membrane permeability. During a normal batch run, aggregates were formed immediately after inoculation, a few spherical aggregates increasing in size during the initial growth phase. At the end of the growth phase, an increase in aggregate concentration was observed, without a considerable increase in aggregate diameter. At the end of the batch run, 160 h after inoculation, aggregates disintegrated into smaller, non-spherical units, following a sharp viability decrease. Cell concentrations of 1.2⋅106 cells/ml were obtained, with 60% of the total cells being in aggregates; the cell concentration in aggregates achieved 5⋅108 cells/ml, with a porosity of 55%. Viability was consistently in the range 85–90%, both for aggregate and suspended cells.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s002530050131

    Articles: DFG German National Licenses
  15. 15
    Staff View
    ISSN:
    1432-0614
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    Abstract Some anchorage-dependent animal cells can form natural aggregates in stirred tanks. Baby hamster kidney (BHK) natural aggregates are described and characterized. Total cell concentration and viability could be obtained after aggregate mechanical aissociation, with negligible cell lysis and no change in cell membrane permeability. During a normal batch run, aggregates were formed immediately after inoculation, a few spherical aggregates increasing size during the initial growth phase. At the end of the growth phase, an increase in aggregate concentration was observed, without a considerable increase in aggregate diameter. At the end of the batch run, 160 h after inoculation, aggregates disintegrated into smaller, non-spherical units, following a sharp viability decrease. Cell concentrations of 1. 2 · 106 cells/ml were obtained, with 60% of the total cells being in aggregates; the cell concentration in aggregates achieved 5 · 108 cells/ml, with a porosity of 55%. Viability was consistently in the range 85–90%, both for aggregate and suspended cells.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00186960

    Articles: DFG German National Licenses
  16. 16
    Staff View
    ISSN:
    1432-0614
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    Abstract The growth and productivity of an Sp2/0 cell line, F3b10, expressing a recombinant antibody (rAb) and BHK21 cells expressing either the same rAb from the same plasmids (BHK.IgG) or secreted alkaline phosphatase (SEAP) (BHK.SEAP) were investigated. The F3b10 line was grown as a single cell suspension. The BHK lines were grown either as suspended natural aggregates or on Cytodex 3 microcarriers. The data for F3b10 showed that the cell-specific rAb production rate (Qs rAb) increased in parallel with increases in the specific growth rate (μ). A similar result was obtained for suspended aggregate cultures of both recombinant BHK cell lines. In contrast, for microcarrier cultures of both BHK cell lines, Qs product increased as μ decreased. This report shows that the relationship between cell growth and Qs product for the cell lines and products studied is dependent upon the culture process. In systems where recombinant cells are growing as a single cell suspension or within a natural suspension aggregate, Qs product increased with increases in μ. In such systems, the cells have a rounded morphology. When cells were grown on microcarriers, Qs product decreased as μ increased. Cells growing attached to a surface are flat and elongated. The observed differences in the relationship of Qs product to μ are correlated with changes in cell morphology. The relationship between Qs product and μ is also affected by the choice of cell line.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF00173987

    Articles: DFG German National Licenses
  17. 17
    Alves, P. ; Soveral, G. ; Macey, R.I. ; Moura, T.F.
    Springer
    Published 1999
    Staff View
    ISSN:
    1432-1424
    Keywords:
    Key words: Eel intestinal vesicles — Elastic properties
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Chemistry and Pharmacology
    Notes:
    Abstract. Changes in volume of intestinal brush border membrane vesicles of the European eel Anguilla anguilla were measured as vesicles were exposed to media with different osmotic pressures. Preparing the vesicles in media of low osmotic pressure allowed the effects of a small hydrostatic pressure to become a significant factor in the osmotic equilibration. By applying LaPlace's law to relate pressure and volume and assuming a linear relation between membrane tension and area expansion, we estimate an initial membrane tension at 4.02 × 10−5 N cm−1 and an area compressibility elastic modulus at 0.87 × 10−3 N cm−1. The elastic modulus estimate falls in the low range of values reported for membranes from other tissues in other species. This lower modulus quantitatively accounts for why eel intestinal vesicles show measurable changes in volume in hypotonic media while rabbit kidney vesicles do not.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s002329900568

    Articles: DFG German National Licenses
  18. 18
    Alves, P. ; Soveral, G. ; Macey, R.I. ; Moura, T.F.
    Springer
    Published 1999
    Staff View
    ISSN:
    1432-1424
    Keywords:
    Key words: Eel intestinal vesicles — Osmotic permeability — Water transport
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Chemistry and Pharmacology
    Notes:
    Abstract. Brush border membrane vesicles, BBMV, from eel intestinal cells or kidney proximal tubule cells were prepared in a low osmolarity cellobiose buffer. The osmotic water permeability coefficient P f for eel vesicles was not affected by pCMBS and was measured at 1.6 × 10−3 cm sec−1 at 23°C, a value lower than 3.6 × 10−3 cm sec−1 exhibited by the kidney vesicles and similar to published values for lipid bilayers. An activation energy E a of 14.7 Kcal mol−1 for water transport was obtained for eel intestine, contrasting with 4.8 Kcal mol−1 determined for rabbit kidney proximal tubule vesicles using the same method of analysis. The high value of E a , as well as the low P f for the eel intestine is compatible with the absence of water channels in these membrane vesicles and is consistent with the view that water permeates by dissolution and diffusion in the membrane. Further, the initial transient observed in the osmotic response of kidney vesicles, which is presumed to reflect the inhibition of water channels by membrane stress, could not be observed in the eel intestinal vesicles. The P f dependence on the tonicity of the osmotic shock, described for kidney vesicles and related to the dissipation of pressure and stress at low tonicity shocks, was not seen with eel vesicles. These results indicate that the membranes from two volume transporter epithelia have different mechanisms of water permeation. Presumably the functional water channels observed in kidney vesicles are not present in eel intestine vesicles. The elastic modulus of the membrane was estimated by analysis of swelling kinetics of eel vesicles following hypotonic shock. The value obtained, 0.79 × 10−3 N cm−1, compares favorably with the corresponding value, 0.87 × 10−3 N cm−1, estimated from measurements at osmotic equilibrium.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/s002329900569

    Articles: DFG German National Licenses
  19. 19
    Staff View
    ISSN:
    1617-4623
    Keywords:
    Neurospora crassa ; Mitochondria ; Complex I ; Assembly ; Gene disruption
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract The nuclear gene coding for the 20.8-kDa subunit of the membrane arm of respiratory chain NADH:ubiquinone reductase (Complex I) fromNeurospora crassa, nuo-20.8, was localized on linkage group I of the fungal genome. A genomic DNA fragment containing this gene was cloned and a duplication was created in a strain ofN. crassa by transformation. To generate RIP (repeat-induced point) mutations in the duplicated sequence, the transformant was crossed with another strain carrying an auxotrophic marker on chromosome I. To increase the chance of finding an isolate with a non-functionalnuo-20.8 gene, random progeny from the cross were selected against this auxotrophy since RIP of the target gene will only occur in the nucleus carrying the duplication. Among these, we isolated and characterised a mutant strain that lacks the 20.8 kDa mitochondrial protein, indicating that this cysteine-rich polypeptide is not essential. Nevertheless, the absence of the 20.8-kDa subunit prevents the full assembly of complex I. It appears that the peripheral arm and two intermediates of the membrane arm of the enzyme are still formed in the mutant mitochondria. The NADH:ubiquinone reductase activity of sonicated mitochondria from the mutant is rotenone insensitive. Electron microscopy of mutant mitochondria does not reveal any alteration in the structure or numbers of the organelles.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/BF02173218

    Articles: DFG German National Licenses
  20. 20
    Alves, P. M. ; Moreira, J. L. ; Rodrigues, J. M. ; Aunins, J. G. ; Carrondo, M. J. T.

    New York, NY [u.a.] : Wiley-Blackwell
    Published 1996
    Staff View
    ISSN:
    0006-3592
    Keywords:
    BHK ; aggregates ; porous microcarriers ; Chemistry ; Biochemistry and Biotechnology
    Source:
    Wiley InterScience Backfile Collection 1832-2000
    Topics:
    Biology
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    The influence of surface growth (two-dimensional microcarriers) and three-dimensional growth (aggregates and macroporous supports) in agitated, suspended batch culture systems upon growth and productivity of BHK was compared. Cultures using three porous microcarriers (CultiSpher G, Cellsnow EX, and Cytocell), one nonporous microcarrier (Cytodex 3) and natural aggregates were performed in stirred tanks using two different agitation rates (60 and 100 RPM). With the exception of Cytocell, cell growth, viability, and productivity were similar when three-dimensional structures (porous microcarriers and aggregates) were used. Nonporous microcarriers only compared well at 60 RPM as growth ceased under overagitation. These results suggest that cultures less susceptible to fluid shear are advantageous for scale-up. © 1996 John Wiley & Sons, Inc.
    Additional Material:
    3 Ill.
    Type of Medium:
    Electronic Resource

    Articles: DFG German National Licenses