Search Results - (Author, Cooperation:O. Madsen)
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1Latest Papers from Table of Contents or Articles in PressLuca De Vico, Andre Anda, Vladimir Al. Osipov, Anders O. Madsen, Thorsten Hansen
National Academy of Sciences
Published 2018Staff ViewPublication Date: 2018-09-26Publisher: National Academy of SciencesPrint ISSN: 0027-8424Electronic ISSN: 1091-6490Topics: BiologyMedicineNatural Sciences in GeneralPublished by: -
2Latest Papers from Table of Contents or Articles in PressM. A. Groenen ; A. L. Archibald ; H. Uenishi ; C. K. Tuggle ; Y. Takeuchi ; M. F. Rothschild ; C. Rogel-Gaillard ; C. Park ; D. Milan ; H. J. Megens ; S. Li ; D. M. Larkin ; H. Kim ; L. A. Frantz ; M. Caccamo ; H. Ahn ; B. L. Aken ; A. Anselmo ; C. Anthon ; L. Auvil ; B. Badaoui ; C. W. Beattie ; C. Bendixen ; D. Berman ; F. Blecha ; J. Blomberg ; L. Bolund ; M. Bosse ; S. Botti ; Z. Bujie ; M. Bystrom ; B. Capitanu ; D. Carvalho-Silva ; P. Chardon ; C. Chen ; R. Cheng ; S. H. Choi ; W. Chow ; R. C. Clark ; C. Clee ; R. P. Crooijmans ; H. D. Dawson ; P. Dehais ; F. De Sapio ; B. Dibbits ; N. Drou ; Z. Q. Du ; K. Eversole ; J. Fadista ; S. Fairley ; T. Faraut ; G. J. Faulkner ; K. E. Fowler ; M. Fredholm ; E. Fritz ; J. G. Gilbert ; E. Giuffra ; J. Gorodkin ; D. K. Griffin ; J. L. Harrow ; A. Hayward ; K. Howe ; Z. L. Hu ; S. J. Humphray ; T. Hunt ; H. Hornshoj ; J. T. Jeon ; P. Jern ; M. Jones ; J. Jurka ; H. Kanamori ; R. Kapetanovic ; J. Kim ; J. H. Kim ; K. W. Kim ; T. H. Kim ; G. Larson ; K. Lee ; K. T. Lee ; R. Leggett ; H. A. Lewin ; Y. Li ; W. Liu ; J. E. Loveland ; Y. Lu ; J. K. Lunney ; J. Ma ; O. Madsen ; K. Mann ; L. Matthews ; S. McLaren ; T. Morozumi ; M. P. Murtaugh ; J. Narayan ; D. T. Nguyen ; P. Ni ; S. J. Oh ; S. Onteru ; F. Panitz ; E. W. Park ; H. S. Park ; G. Pascal ; Y. Paudel ; M. Perez-Enciso ; R. Ramirez-Gonzalez ; J. M. Reecy ; S. Rodriguez-Zas ; G. A. Rohrer ; L. Rund ; Y. Sang ; K. Schachtschneider ; J. G. Schraiber ; J. Schwartz ; L. Scobie ; C. Scott ; S. Searle ; B. Servin ; B. R. Southey ; G. Sperber ; P. Stadler ; J. V. Sweedler ; H. Tafer ; B. Thomsen ; R. Wali ; J. Wang ; S. White ; X. Xu ; M. Yerle ; G. Zhang ; J. Zhang ; S. Zhao ; J. Rogers ; C. Churcher ; L. B. Schook
Nature Publishing Group (NPG)
Published 2012Staff ViewPublication Date: 2012-11-16Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Animals ; Demography ; Genome/*genetics ; Models, Animal ; Molecular Sequence Data ; *Phylogeny ; Population Dynamics ; Sus scrofa/*classification/*geneticsPublished by: -
3Articles: DFG German National LicensesStaff View
ISSN: 1365-2958Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyMedicineNotes: The expression of Escherichia coli type 1 fimbriae is phase-variable i.e. the bacterial cell is either fimbriated or non-fimbriated. The transition from one state to the other is caused by the change in configuration of an invertible DNA segment harbouring the promoter of the fimA gene. The position of this phase switch is controlled by two proteins, FimB and FimE, which mediate an ‘on’ or ‘off’ configuration of the switch, respectively. In this study, we have investigated how these proteins control the switch by means of fim-lac fusions on low-copy-number plasmids. It was found, by in trans and cis complementation, that the ratio of fimB to fimE and the total concentration of the gene products determine the configuration of the switch as well as the frequency of phase switching. It was also shown that transcription occurs from the promoter located at the phase switch when this is in the ‘off’ configuration. This suggests a regulatory mechanism, since the resulting transcript would be anti-sense to the fimE transcript.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 0014-4827Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 0006-291XSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyPhysicsType of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesMadsen, O. D. ; Olsson, M. Landin ; Bille, G. ; Sundkvist, G. ; Lemmark, Å. ; Dahlqvist, G. ; Ludvigsson, J.
Springer
Published 1986Staff ViewISSN: 1432-0428Keywords: Islet cell antibody assay ; two-colour assay ; human proinsulin antibodySource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary The conventional indirect immunofluorescence assay for islet cell antibodies was compared with a two-colour immunofluorescent assay to detect both islet cell antibodies with fluorescein isothiocyanate-labeled rabbit anti-human IgG and pancreatic B cells with a monoclonal human proinsulin antibody and Texas red-labeled sheep anti-mouse IgG. Determinations of end-point titres showed a correlation between the new two-colour immunofluorescent assay and the conventional indirect immunofluorescent assay in 1) selected sera positive for islet cell antibodies and insulin autoantibodies r s= 0.93 (p〈0.01) or for islet cell antibodies alone r s=0.99 (p〈0.005) and 2) sera from children or young adults with newly diagnosed Type 1 (insulin-dependent) diabetes r s=0.95 (p〈0.0001). No interference between the monoclonal human proinsulin antibodies and islet cell antibodies with or without insulin autoantibodies or between the two second fluorescent antibodies was detected. It is concluded that the two-colour immunofluorescence assay is advantageous since a) it is possible to mix the reagents to avoid a more time-consuming and technically complicated assay, b) the presence of B cells can be confirmed in each section to permit detection of B cell cytoplasmic antibodies and c) microscopic evaluation is easier and more accurate, particulary in islet cell antibody negative samples.Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 1432-0428Keywords: Islet amyloid polypeptide ; fetal pancreas ; immunocytochemistry ; distribution ; age dependenceSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary Islet amyloid polypeptide is known to localize to the adult human Beta cell. We analysed the immunoreactivity for islet amyloid polypeptide in a series of 29 human fetal pancreata (9–24 weeks of gestation) with respect to age dependency and cellular localization using an antibody raised against synthetic rat islet amyloid polypeptide 12–37. Cells immunoreactive for islet amyloid polypeptide were demonstrated in low numbers from week 13 onwards while insulin positivity was already present at 9 weeks of gestation. In the age group 13–16 gestational weeks, cells positive for insulin were 20-fold more frequent than cells positive for islet amyloid polypeptide. This difference gradually disappeared with age, reaching parity in the adult gland. Double immunostaining demonstrated that all islet amyloid polypeptide immunoreactivity co-localized with insulin. Co-expression of insulin and islet amyloid polypeptide was more frequent in Beta-cell clusters (≥10 cells) than in single Beta cells; islet amyloid polypeptide positivity was present in 58±9% (mean±SEM; n=4) of fetal, 88±9 % (n=3) of neonatal and 100% (n=3) of adult clustered Beta cells, and only 8–18 % of the single Beta cells. The results suggest that the developing fetal Beta cells, dependent on age and localization, differ in their capacity to express detectable amounts of immunoreactive islet amyloid polypeptide. Beta-cell maturation might therefore be associated with islet amyloid polypeptide expression.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 1432-0428Keywords: Glucagon ; insulin ; glucose tolerance ; diabetes mellitusSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary The effect of peripheral and intraportal infusions of 0.86 pmol/kg · min−1 of glucagon on plasma glucose, plasma insulin, and glucose tolerance was examined in four normal subjects. Peripheral glucagon concentrations increased by 60–90 pmol/l during intraportal and 70–180 pmol/l during peripheral infusions. The infusions caused increases in plasma glucose levels of approximately 1 mmol/l, and in plasma insulin levels of 75–100%, regardless of route of administration. Intravenous glucose tolerance tests carried out during the glucagon infusions showed that glucose tolerance remained within the normal range and was uninfluenced by the route of administration.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1439-0973Source: Springer Online Journal Archives 1860-2000Topics: MedicineDescription / Table of Contents: Zusammenfassung Bei 50 nacheinander zur transurethralen Prostataresektion eingewiesenen Patienten wurden bakteriologische Untersuchungen durchgeführt. Präoperativ hatten 28% der Patienten eine asymptomatische Bakteriurie. In der postoperativen Phase wurde bei 46% der Patienten eine vorübergehende Bakteriämie nachgewiesen. Patienten, die wegen Prostatahypertrophie operiert wurden, wiesen signifikant häufiger eine Bakteriämie auf als Patienten mit Prostatakarzinom und Patienten, bei denen ein langdauernder chirurgischer Eingriff erforderlich war. Patienten, bei denen pathogene Bakterien im Blut nachgewiesen wurden, mußten signifikant länger stationär behandelt werden.Notes: Summary A bacteriological survey of 50 consecutive patients undergoing transurethral resection of the prostate was performed. Preoperatively, 28% of the patients had asymptomatic bacteriuria. In the postoperative period, 46% of all the patients developed transient bacteremia. A significantly higher rate of bacteremia was found in patients with hypertrophy of the prostate than in those with cancer of the prostate and in patients undergoing long-lasting surgical intervention. Patients who developed bacteremia due to pathogenic bacteria were hospitalized for a significantly longer period of time.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 1434-9949Keywords: Osteoarthrosis ; Muscle Strength ; Walking Time ; ReproducibilitySource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary The intra-session and inter-session reproducibility of knee extensor and flexor strength measurements were examined in 21 gonarthrotic subjects (ten women and eleven men). Using the Cybex 6000 dynamometer, isokinetic peak torque and total work at 30 and 120 degrees/second and isometric peak torque were measured three times on separate days within two weeks by the same examiner. The reproducibility of walking and stair climbing time measurements was also assessed. The concept of critical difference (i.e. the difference between two measurements which would be statistically significant when applied to a reference group in steady state) for the interpretation of muscle strength data obtained by monitoring individual patients is presented. Individual coefficients of variation (CV) were calculated for each muscle strength variable. Depending on the velocity and on whether peak torque or total work were measured, the median CV of intra-session and inter-session extensor strength measurements ranged from 1.5–4.9% and 7.4–10.1%, respectively. CVs for flexor strength measurements were significantly higher. Substantial variability of within subject variances were found, e.g. the 80% central range of CVs for extensor toruqe at 30 degrees/second was 2.5–29.5% (inter-session). Calculated from CVs, critical differences for inter-session measurements exceeded 30% for all muscle strength variables. Median CVs for walking and stair-climbing time were 7.0% and 4.9%, respectively. In conclusion, the large CVs and corresponding critical differences may be a major limitation in the use of muscle strength measurements in the individual gonarthrotic patient.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 1434-9949Keywords: Body Composition ; Bone Mineral Density ; Fat Mass ; Muscle Strength ; Osteoarthritis ; PainSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary It is unclear whether patients with knee osteoarthritis (OA) and hip OA differ regarding soft tissue composition and bone mineral density (BMD). A total of 42 women waiting for a replacement of the hip (n=20) or the knee (n=22) due to OA were examined. Fat mass (FM), percent body fat (%fat), lean mass (LM) and BMD were measured by dual energy X-ray absorptiometry (DEXA). Knee extensor and flexor strength was measured by an isokinetic dynamometer. No significant differences in age, height, disease duration, Lequesne score or pain scores were found between the groups. Comparing the radiographic changes of the knees with those of the hips, changes were most severe in the joints which were to be replaced. Body weight, body mass index, total and regional FM, and %fat were more than 15% higher in patients waiting for a knee replacement (p〈0.001). Also lean mass tended to be higher in the knee patients. Differences in BMD did not remain statistically significant after correction for body weight. Muscle strength was similar in the two groups but was reduced by 20% in the legs in which the joint was to be replaced compared to the contralateral legs. However, the mean difference in lean mass between the two legs was only 3% (p〈0.05). The scores for pain felt during strength testing were significantly higher for the involved legs than for the contralateral legs. In conclusion, fat mass values were considerably higher in patients scheduled for a knee replacement. Impaired strength performance in OA may be more strongly associated with pain than with reduced muscle mass.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesStaff View
ISSN: 1434-9949Keywords: Osteoarthrosis ; Muscle Strength ; Disability ; Radiology ; Bone ; BMD ; PainSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary Patients with osteoarthrosis suffer from disability and pain. We measured isokinetic and isometric peak torque in 20 women with gonarthrosis (GA) and in 26 healthy controls. Relationships between muscle strength, walking and stair climbing time, pain level and pain disability scores as assessed by the patients using an extensive questionnaire, radiological changes and subchondral sclerosis expressed as bone mineral density (BMD, g/cm2) of the proximal tibia were studied. Precision errors of the torque measurements in both GA patients and controls were approximately 6%. In the GA patients, isokinetic and isometric quadriceps strength was reduced by 40 and 15% (p〈0.01) respectively, and walking and stair climbing time was increased by 30% (p〈0.005). Isokinetic strength was a better predictor of pain level and pain disability scores than isometric strength and radiological stage. Walking time and stair climbing time were not associated with quadriceps strength, pain level, pain disability scores or radiological changes. Subchondral BMD was not predictive of pain. The study suggests that quadriceps strength assessed by isokinetic dynamometry in GA is a reliable measurement. Isokinetic strength was pronouncedly reduced compared to isometric strength and was a more important predictor of pain and pain disability than isometric strength. These findings should be taken into consideration when planning exercise studies and programmes in GA.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1432-0525Source: Springer Online Journal Archives 1860-2000Topics: Computer ScienceNotes: Summary To improve the readability of a grammar it is common to use extended context free grammars (ECFGs) which are context free grammars (CFGs) extended with the repetition operator (*), the alternation operator (¦) and parentheses to express the right hand sides of the productions. The topic treated here is LR-parsing of ECFGs. The LR(k) concept is generalized to ECFGs, a set of LR-preserving transformations from ECFGs to CFGs is given and finally it is shown how to construct LR-parsers directly from ECFGs.Type of Medium: Electronic ResourceURL: