Search Results - (Author, Cooperation:Nhat Le)

1432-0851

Bispecific antibody ; Redirected lysis ; Targeted cytotoxicity ; Mammary tumor ; Mouse model

Springer Online Journal Archives 1860-2000

Medicine

Abstract Bispecific antibodies with specificity for CD3 and a tumor antigen can redirect cytolytic T cells to kill tumor targets, regardless of their natural specificity. To assess the clinical potential of bispecific antibodies for treatment of human cancers we have, in the present study, adapted a totally syngeneic mouse model to the targeting of mouse T cells against mouse tumors in immunocompetent mice. We show that gp52 of the mouse mammary tumor virus (MTV) can serve as a tumor-specific antigen for redirected cellular cytotoxicity. Chemically crosslinked and genetically engineered bispecific antibodies with specificities for gp52 and murine CD3 ε-chain induced activated mouse T cells to specifically lyse mouse mammary tumor cells from cultured lines and primary tumors from C3H-MTV+ mice. Retargeted T cells also blocked the growth of mammary tumors in vitro as well as their growth in syngeneic mice. These findings identify murine MTV-induced mammary adenocarcinomas as a solid-tumor, animal model for retargetin T cells with bispecific antibodies against syngeneic breast cancer.

Electronic Resource

1089-7690

AIP Digital Archive

Physics

Chemistry and Pharmacology

We have studied both the vertical–vertical and vertical–horizontal dynamic light scattering spectra of triphenylphosphite over a wide range of viscosities, temperatures, densities, and pressures. In particular, we have focused on the propagating modes, both the longitudinal (Brillouin) and the transverse (shear) modes. We find that the linewidths of the associated spectral side peaks can be described over the entire range, from low viscosity hydrodynamic to high viscosity viscoelastic behavior, as a function solely of viscosity. The same is true for the frequency shifts of the shear waves but not for those of the longitudinal waves. We give an interpretation of these results. We also find that at a given temperature the shifts are unexpectedly linear in pressure over the entire range; this, we believe, is not a consequence of the pressure dependence of the adiabatic sound speed, but rather of a frequency-dependent viscosity with a broad distribution of relaxation frequencies. Our analysis suggests that the low-frequency longitudinal and shear viscosities are rather similar.

Electronic Resource

1432-0851

Key words Bispecific antibody ; Redirected lysis ; Targeted cytotoxicity ; Mammary tumor ; Mouse model

Springer Online Journal Archives 1860-2000

Medicine

Abstract  Bispecific antibodies with specificity for CD3 and a tumor antigen can redirect cytolytic T cells to kill tumor targets, regardless of their natural specificity. To assess the clinical potential of bispecific antibodies for treatment of human cancers we have, in the present study, adapted a totally syngeneic mouse model to the targeting of mouse T cells against mouse tumors in immunocompetent mice. We show that gp52 of the mouse mammary tumor virus (MTV) can serve as a tumor-specific antigen for redirected cellular cytotoxicity. Chemically crosslinked and genetically engineered bispecific antibodies with specificities for gp52 and murine CD3 ɛ-chain induced activated mouse T cells to specifically lyse mouse mammary tumor cells from cultured lines and primary tumors from C3H-MTV+ mice. Retargeted T cells also blocked the growth of mammary tumors in vitro as well as their growth in syngeneic mice. These findings identify murine MTV-induced mammary adenocarcinomas as a solid-tumor, animal model for retargeting T cells with bispecific antibodies against syngeneic breast cancer.

Electronic Resource