Search Results - (Author, Cooperation:N. Rajagopal)
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1Latest Papers from Table of Contents or Articles in PressM. D. Schultz ; Y. He ; J. W. Whitaker ; M. Hariharan ; E. A. Mukamel ; D. Leung ; N. Rajagopal ; J. R. Nery ; M. A. Urich ; H. Chen ; S. Lin ; Y. Lin ; I. Jung ; A. D. Schmitt ; S. Selvaraj ; B. Ren ; T. J. Sejnowski ; W. Wang ; J. R. Ecker
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-06-02Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Age Factors ; Alleles ; Chromosome Mapping ; *DNA Methylation ; *Epigenesis, Genetic ; Female ; Gene Expression Profiling ; Gene Expression Regulation ; Genetic Variation ; Humans ; Male ; Organ SpecificityPublished by: -
2Latest Papers from Table of Contents or Articles in PressJ. R. Dixon ; I. Jung ; S. Selvaraj ; Y. Shen ; J. E. Antosiewicz-Bourget ; A. Y. Lee ; Z. Ye ; A. Kim ; N. Rajagopal ; W. Xie ; Y. Diao ; J. Liang ; H. Zhao ; V. V. Lobanenkov ; J. R. Ecker ; J. A. Thomson ; B. Ren
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-02-20Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Alleles ; Allelic Imbalance/genetics ; *Cell Differentiation/genetics ; Cell Lineage/genetics ; Chromatin/*chemistry/genetics/*metabolism ; *Chromatin Assembly and Disassembly/genetics ; Embryonic Stem Cells/*cytology/*metabolism ; Enhancer Elements, Genetic/genetics ; Epigenesis, Genetic/*genetics ; Epigenomics ; Gene Regulatory Networks ; Humans ; Promoter Regions, Genetic/genetics ; Reproducibility of ResultsPublished by: -
3Latest Papers from Table of Contents or Articles in PressD. Leung ; I. Jung ; N. Rajagopal ; A. Schmitt ; S. Selvaraj ; A. Y. Lee ; C. A. Yen ; S. Lin ; Y. Lin ; Y. Qiu ; W. Xie ; F. Yue ; M. Hariharan ; P. Ray ; S. Kuan ; L. Edsall ; H. Yang ; N. C. Chi ; M. Q. Zhang ; J. R. Ecker ; B. Ren
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-02-20Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Acetylation ; *Alleles ; Chromatin/genetics/metabolism ; Chromosomes, Human/genetics ; Datasets as Topic ; Enhancer Elements, Genetic/genetics ; Epigenesis, Genetic/*genetics ; *Epigenomics ; Genetic Variation/genetics ; Haplotypes/*genetics ; Histones/metabolism ; Humans ; Nucleotide Motifs ; Organ Specificity/genetics ; Transcription, Genetic/geneticsPublished by: -
4Latest Papers from Table of Contents or Articles in PressA. Kundaje ; W. Meuleman ; J. Ernst ; M. Bilenky ; A. Yen ; A. Heravi-Moussavi ; P. Kheradpour ; Z. Zhang ; J. Wang ; M. J. Ziller ; V. Amin ; J. W. Whitaker ; M. D. Schultz ; L. D. Ward ; A. Sarkar ; G. Quon ; R. S. Sandstrom ; M. L. Eaton ; Y. C. Wu ; A. R. Pfenning ; X. Wang ; M. Claussnitzer ; Y. Liu ; C. Coarfa ; R. A. Harris ; N. Shoresh ; C. B. Epstein ; E. Gjoneska ; D. Leung ; W. Xie ; R. D. Hawkins ; R. Lister ; C. Hong ; P. Gascard ; A. J. Mungall ; R. Moore ; E. Chuah ; A. Tam ; T. K. Canfield ; R. S. Hansen ; R. Kaul ; P. J. Sabo ; M. S. Bansal ; A. Carles ; J. R. Dixon ; K. H. Farh ; S. Feizi ; R. Karlic ; A. R. Kim ; A. Kulkarni ; D. Li ; R. Lowdon ; G. Elliott ; T. R. Mercer ; S. J. Neph ; V. Onuchic ; P. Polak ; N. Rajagopal ; P. Ray ; R. C. Sallari ; K. T. Siebenthall ; N. A. Sinnott-Armstrong ; M. Stevens ; R. E. Thurman ; J. Wu ; B. Zhang ; X. Zhou ; A. E. Beaudet ; L. A. Boyer ; P. L. De Jager ; P. J. Farnham ; S. J. Fisher ; D. Haussler ; S. J. Jones ; W. Li ; M. A. Marra ; M. T. McManus ; S. Sunyaev ; J. A. Thomson ; T. D. Tlsty ; L. H. Tsai ; W. Wang ; R. A. Waterland ; M. Q. Zhang ; L. H. Chadwick ; B. E. Bernstein ; J. F. Costello ; J. R. Ecker ; M. Hirst ; A. Meissner ; A. Milosavljevic ; B. Ren ; J. A. Stamatoyannopoulos ; T. Wang ; M. Kellis
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-02-20Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Base Sequence ; Cell Lineage/genetics ; Cells, Cultured ; Chromatin/chemistry/genetics/metabolism ; Chromosomes, Human/chemistry/genetics/metabolism ; DNA/chemistry/genetics/metabolism ; DNA Methylation ; Datasets as Topic ; Enhancer Elements, Genetic/genetics ; Epigenesis, Genetic/*genetics ; *Epigenomics ; Genetic Variation/genetics ; Genome, Human/*genetics ; Genome-Wide Association Study ; Histones/metabolism ; Humans ; Organ Specificity/genetics ; RNA/genetics ; Reference ValuesPublished by: -
5Latest Papers from Table of Contents or Articles in PressM. D. Schultz ; Y. He ; J. W. Whitaker ; M. Hariharan ; E. A. Mukamel ; D. Leung ; N. Rajagopal ; J. R. Nery ; M. A. Urich ; H. Chen ; S. Lin ; Y. Lin ; I. Jung ; A. D. Schmitt ; S. Selvaraj ; B. Ren ; T. J. Sejnowski ; W. Wang ; J. R. Ecker
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-11-26Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsPublished by: -
6Articles: DFG German National LicensesStaff View
ISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] RECENT investigations of the sociology of science have suggested a major shift in the organization of science and scientists. The general suggestion is that although science previously was a closed system1, now it is not so. Science is now a part of society and, as such, is subjected to the same ...Type of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesAagaard, Claus ; Leviev, Ilia ; Aravalli, Rajagopal N. ; Forterre, Patrick ; Prieur, Daniel ; Garrett, Roger A.
Oxford, UK : Blackwell Publishing Ltd
Published 1996Staff ViewISSN: 1574-6976Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: BiologyNotes: Abstract: Although there are currently no cloning and expression vectors available for archaeal hyperthermophiles, small cryptic plasmids have been characterized for these organisms as well as viruses and introns capable of spreading between cells. Below, we review the recent progress in adapting these genetic elements as vectors for Pyrococcus furiosus and Sulfolobus acidocaldarius. An efficient and reliable transformation procedure is described for both organisms. The potential of the mobile intron from Desulfurococcus mobilis, inserted into the bacterial vector pUC18 to generate a new type of vector, was investigated in S. acidocaldarius. A polylinker was inserted upstream from the open reading frame encoding the homing enzyme I-DmoI. Both the polylinker and a 276 bp fragment of the tetracycline gene from pBR322 could be inserted into the intron-plasmid construct and spreading still occurred in the culture of S. acidocaldarius. Experiments are in progress to test the co-mobility of the alcohol dehydrogenase and β-galactosidase genes from Sulfolobus species with the intron. A shuttle vector pCSV1 was also produced by fusing the pGT5 plasmid from Pyrococcus abyssi and the bacterial vector pUC19 which, on transformation, is stable in both organisms without selection. Growth inhibition studies indicate that both P. furiosus and S. acidocaldarius are sensitive to the antibiotics carbomycin, celesticetin, chloramphenicol and thiostrepton as well as butanol and butylic alcohol. Spontaneous mutants resistant to these drugs have been isolated carrying single site mutations in their 23S rRNA gene; they include mutants of S. acidocaldarius resistant to chloramphenicol, carbomycin and celesticetin with the mutation C2452U and thiostrepton-resistant mutants of P. furiosus carrying the mutation A1067G (both numbers corresponding to Escherichia coli 23S rRNA). These mutated genes are being developed as selective markers. Moreover, two β-galactosidase genes from P. furiosus have been cloned as possible phenotypic markers; one of these exhibits maximum activity at 95°C with O-nitrophenyl β-d-galactopyranoside as substrate.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesRAHMAN, A. ; GHOSAL, A. ; SEN, N. ; RAJAGOPAL, N. R. ; GUPTA, S. DAS ; HUSSAINI, S. H. M. ; ROY, A. K.
[s.l.] : Nature Publishing Group
Published 1964Staff ViewISSN: 1476-4687Source: Nature Archives 1869 - 2009Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsNotes: [Auszug] THE post-war period saw a rapid growth of scientific research in India. The Council of Scientific and Industrial Research which was set up in the early 'forties set up a chain of thirty-one laboratories during the period 194663. These laboratories were committed to the task of solving industrial ...Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 1433-4909Keywords: Key words Archaea ; Hyperthermophile ; Pyrococcus Sulfolobus ; Shuttle vector ; Alcohol dehydrogenaseSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Progress in understanding the basic molecular, biochemical, and physiological characteristics of archaeal hyperthermophiles has been limited by the lack of suitable expression vectors. Here, we report the construction of versatile shuttle vectors that can be maintained, and selected for, in both archaea and bacteria. The primary construct, pAG1, was produced by ligating portions of the archaeal cryptic plasmid pGT5 and the bacterial plasmid pUC19, both of which exhibit high copy numbers. A second vector construct, pAG2, was generated, with a reduced copy number in Escherichia coli, by introducing the Rom/Rop gene from pBR322 into pAG1. After transformation, both pAG1 and pAG2 were stably maintained and propagated in the euryarchaeote Pyrococcus furiosus, the crenarchaeote Sulfolobus acidocaldarius, and in Escherichia coli. An archaeal selective marker, the alcohol dehydrogenase gene from Sulfolobus solfataricus, was isolated by polymerase chain reaction (PCR) amplification and cloned into the two constructs. They were stably maintained and expressed in the two archaea and conferred resistance to butanol and benzyl alcohol. However, the vector pAG21, deriving from pAG2, proved the more stable in E. coli probably due to its lower copy number in the bacterium. Conditions are presented for the use of the vectors which, potentially, can be used for other hyperthermophilic archaea.Type of Medium: Electronic ResourceURL: