Search Results - (Author, Cooperation:M. Thomm)

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  1. 1
    Staff View
    Publication Date:
    2012-02-24
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    ATP-Binding Cassette Transporters/chemistry/metabolism ; Cell Cycle Proteins/chemistry/metabolism ; Cryoelectron Microscopy ; Endoribonucleases/chemistry/metabolism ; *Evolution, Molecular ; Iron-Sulfur Proteins/chemistry/metabolism ; Models, Molecular ; Movement ; Multiprotein Complexes/chemistry/metabolism ; Nuclear Proteins/chemistry/metabolism ; Peptide Termination Factors/chemistry/metabolism ; Protein Binding ; Protein Stability ; Protein Structure, Tertiary ; Pyrococcus furiosus/*chemistry/metabolism ; Ribosomes/*chemistry/*metabolism/ultrastructure ; Saccharomyces cerevisiae/*chemistry/metabolism ; Saccharomyces cerevisiae Proteins/chemistry/metabolism
    Published by:
    http://www.nature.com/

    Latest Papers from Table of Contents or Articles in Press
  2. 2
    Rothe, Oliver ; Thomm, M.
    Springer
    Published 2000
    Staff View
    ISSN:
    1433-4909
    Keywords:
    Key words Archaea ; Anaerobiosis ; Reducing agents ; Sulfite ; Plating efficiency ; Anaerobic chamber ; Methanogens ; Hyperthermophiles
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Notes:
    Abstract The extreme sensitivity of many Archaea to oxygen is a major obstacle for their cultivation in the laboratory and the development of archaeal genetic exchange systems. The technique of Balch and Wolfe (1976) is suitable for the cultivation of anaerobic Archaea but involves time-consuming procedures such as the use of air locks and glove boxes. We describe here a procedure for the cultivation of anaerobic Archaea that is more convenient and faster and allows the preparation of liquid media without the use of an anaerobic chamber. When the reducing agent sodium sulfide (Na2S) was replaced by sodium sulfite (Na2SO3), anaerobic media could be prepared without protection from oxygen outside an anaerobic chamber. Exchange of the headspace of serum bottles by appropriate gases was sufficient to maintain anaerobic conditions in the culture media. Organisms that were unable to utilize sulfite as a source for cellular sulfur were supplemented with hydrogen sulfide. H2S was simply added to the headspace of serum bottles by a syringe. The use of H2S as a source for sulfur minimized the precipitation of cations by sulfide. Representatives of 12 genera of anaerobic Archaea studied here were able to grow in media prepared by this procedure. For the extremely oxygen-sensitive organism Methanococcus thermolithotrophicus, we show that plates could be prepared outside an anaerobic chamber when sulfite was used as reducing agent. The application of this method may faciliate the cultivation and handling of extreme anaerobic Archaea considerably.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1007/PL00010716

    Articles: DFG German National Licenses