Search Results - (Author, Cooperation:M. Rozman)
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1Latest Papers from Table of Contents or Articles in PressX. S. Puente ; S. Bea ; R. Valdes-Mas ; N. Villamor ; J. Gutierrez-Abril ; J. I. Martin-Subero ; M. Munar ; C. Rubio-Perez ; P. Jares ; M. Aymerich ; T. Baumann ; R. Beekman ; L. Belver ; A. Carrio ; G. Castellano ; G. Clot ; E. Colado ; D. Colomer ; D. Costa ; J. Delgado ; A. Enjuanes ; X. Estivill ; A. A. Ferrando ; J. L. Gelpi ; B. Gonzalez ; S. Gonzalez ; M. Gonzalez ; M. Gut ; J. M. Hernandez-Rivas ; M. Lopez-Guerra ; D. Martin-Garcia ; A. Navarro ; P. Nicolas ; M. Orozco ; A. R. Payer ; M. Pinyol ; D. G. Pisano ; D. A. Puente ; A. C. Queiros ; V. Quesada ; C. M. Romeo-Casabona ; C. Royo ; R. Royo ; M. Rozman ; N. Russinol ; I. Salaverria ; K. Stamatopoulos ; H. G. Stunnenberg ; D. Tamborero ; M. J. Terol ; A. Valencia ; N. Lopez-Bigas ; D. Torrents ; I. Gut ; A. Lopez-Guillermo ; C. Lopez-Otin ; E. Campo
Nature Publishing Group (NPG)
Published 2015Staff ViewPublication Date: 2015-07-23Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: 3' Untranslated Regions/genetics ; Alternative Splicing/genetics ; B-Cell-Specific Activator Protein/biosynthesis/genetics ; B-Lymphocytes/metabolism ; Carrier Proteins/genetics ; Chromosomes, Human, Pair 9/genetics ; DNA Mutational Analysis ; DNA, Neoplasm/genetics ; Enhancer Elements, Genetic/genetics ; Genomics ; Humans ; Leukemia, Lymphocytic, Chronic, B-Cell/*genetics/metabolism/pathology ; Mutation/*genetics ; Nerve Tissue Proteins/genetics ; Nuclear Proteins/genetics ; Protein Tyrosine Phosphatase, Non-Receptor Type 11/genetics ; Receptor, Notch1/genetics/metabolism ; Transcription Factors/geneticsPublished by: -
2Latest Papers from Table of Contents or Articles in PressX. S. Puente ; M. Pinyol ; V. Quesada ; L. Conde ; G. R. Ordonez ; N. Villamor ; G. Escaramis ; P. Jares ; S. Bea ; M. Gonzalez-Diaz ; L. Bassaganyas ; T. Baumann ; M. Juan ; M. Lopez-Guerra ; D. Colomer ; J. M. Tubio ; C. Lopez ; A. Navarro ; C. Tornador ; M. Aymerich ; M. Rozman ; J. M. Hernandez ; D. A. Puente ; J. M. Freije ; G. Velasco ; A. Gutierrez-Fernandez ; D. Costa ; A. Carrio ; S. Guijarro ; A. Enjuanes ; L. Hernandez ; J. Yague ; P. Nicolas ; C. M. Romeo-Casabona ; H. Himmelbauer ; E. Castillo ; J. C. Dohm ; S. de Sanjose ; M. A. Piris ; E. de Alava ; J. San Miguel ; R. Royo ; J. L. Gelpi ; D. Torrents ; M. Orozco ; D. G. Pisano ; A. Valencia ; R. Guigo ; M. Bayes ; S. Heath ; M. Gut ; P. Klatt ; J. Marshall ; K. Raine ; L. A. Stebbings ; P. A. Futreal ; M. R. Stratton ; P. J. Campbell ; I. Gut ; A. Lopez-Guillermo ; X. Estivill ; E. Montserrat ; C. Lopez-Otin ; E. Campo
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-06-07Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Amino Acid Sequence ; Animals ; Carrier Proteins/genetics ; DNA Mutational Analysis ; Genome, Human/*genetics ; Humans ; Karyopherins/genetics ; Leukemia, Lymphocytic, Chronic, B-Cell/*genetics ; Molecular Sequence Data ; Mutation/*genetics ; Myeloid Differentiation Factor 88/chemistry/genetics ; Receptor, Notch1/genetics ; Receptors, Cytoplasmic and Nuclear/genetics ; Reproducibility of ResultsPublished by: -
3Articles: DFG German National LicensesXAUBET, A. ; MULLOL, J. ; LÓPEZ, E. ; ROCA-FERRER, J. ; ROZMAN, M. ; CARRIÖN, T. ; FABRA, J. M. ; PICADO, C.
Oxford, UK : Blackwell Publishing Ltd
Published 1994Staff ViewISSN: 1365-2222Source: Blackwell Publishing Journal Backfiles 1879-2005Topics: MedicineNotes: Eosinophilic infiltration of the respiratory mucosa is considered an inflammatory hallmark of allergic rhinitis, bronchial asthma and nasal polyposis. However, the mechanisms involved in this infiltration have not yet been totally elucidated. The objective of this study was to investigate and compare the influence of epithelial cell secretions from both nasal polyps (NP) and normal nasal mucosa (NM) on in vitro eosinophil survival. Epithelial cells were identified by microscopy; and immunohislochemisiry. cultured to confluence, and human epithelial cell conditioned media (HECM) was generated from cultures. Eosinophits were isolated at high viability and purity (〉90%) from peripheral blood and incubated with HECM. HECM from both NM and NP increased eosinophil survival in a dose-dependent manner, this effect being maximal at a concentration of 25% for NM (73.4%±5.5%, n= 26, P〈 0.001) and of 10% for NP (74.5%± 84%n= 18, P 〈 0.001). Incubation of monoclonal antibody to human GM-CSF with HECM, neutralized the induction of eosinophil survival by HECM from both NM and NP. HECM from NP contained higher concentrations of GM-CSF (111 ± 25.4 pg/ml, n= 17) than HECM from NM (97.1 ± 15.2 pg/ml. n= 8). without reaching statistical significance. Pre-incubation of dexamethasone with eosinophils also blocked HFCM-induced eosinophil survival from both NM (10−8-10−5 M; IC50 = 9.5 nM) and NP (10-7-10-5 M; IC50 = 83 nM). These results suggest that: firstly eosinophil infiltration into the respiratory mucosa during allergic reaction and nasal polyposis may be modulated at least in part by GM-CSF from epithelial cells; and secondly epithelial cells from NP might have a more potent effect on inducing eosinophil infiltration of the respiratory mucosa than epithelial cells from NM. Finally, we may consider this as a reliable in vitro model to compare the role of epithelial cells from inflammatory (NP) and non-inflammatory (NM) tissue in respiratory inflammation.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 0030-4018Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 0375-9601Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 0030-4018Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: PhysicsType of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 1432-0584Keywords: Chronic myeloid leukemia ; Neutrophil granulocytes ; PrognosisSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary To ascertain whether progression from the chronic to the accelerated and blastic phases of chronic myeloid leukemia (CML) is associated with the loss of the myeloid differentiation antigens of the neutrophil granulocytes (NG), we analyzed two monoclonal antibodies (PMN 31D8 and PMN 13F6) recognizing normal peripheral blood NG membrane antigens in 49 patients in different evolutive stages of CML. Since five patients were studied twice, a total of 54 studies were carried out. Fourteen patients were evaluated at diagnosis, 12 in the controlled chronic phase within 1 year from diagnosis, 14 in the advanced chronic phase (median evolution 3.25 years), and 14 in the accelerated (five cases) or blastic (nine cases) phases. Fourteen normal subjects served as a control group. At diagnosis, a significant decrease in the positivity for both antigens was observed with respect to controls, probably due to the circulation of incompletely mature NG. In the early chronic phase the values were within the normal range, whereas a significant decrease was registered in the advanced chronic phase and especially in the accelerated/blastic phase. A negative correlation between the NG positivity for both markers and the time elapsed from the moment of obtaining the initial control of the disease was found, suggesting that a progressive loss of the myeloid antigens of the NG occurs during the evolutive course of CML. These results seem to confirm the usefulness of the NG myeloid differentiation antigen study as an evolutive parameter in CML.Type of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesWoessner, S. ; Feliu, E. ; Villamor, N. ; Zarco, M. A. ; Domingo, A. ; Millá, F. ; Florensa, L. ; Rozman, M. ; Abella, E. ; Soler, J. ; Vallespí, T. ; Irriguible, M. D. ; Solé, F.
Springer
Published 1994Staff ViewISSN: 1432-0584Keywords: Granular lymphocytes ; Proliferative disorders ; T lymphocytes ; NK cellsSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary A series of 20 patients with granular lymphocyte proliferative disorders (GLPD) is reported. The criterion of inclusion was presence of persistent (≥6 months) granular lymphocytosis in the absence of any causative illness. Diagnoses made upon analytical control in half the patients of splenomegaly (25%) and hepatomegaly (25%) were infrequent. Clinical course was nonprogressive in 17/20 patients, but two developed high-grade NHL several years later and one showed progressive disease. Actuarial probability of survival at 5 years was 85%. Granular lymphocyte morphology was relatively homogeneous, and peripheral blood counts were preserved in the most patients. Bone marrow lymphocytic infiltration was low, as assessed by bone marrow aspiration and/or biopsy. Eosinophilia was an outstanding feature in eight cases. Ultrastructurally, all cases showed parallel tubular arrays; cytoplasmic granules and numerous short microvilli were noticed. The lymphoid phenotype was heterogeneous, the most common being CD2+CD3+CD4-CD8+, but six patients (30%) were CD4+ with variable expression of natural killer-associated antigens. Chromosomal analysis was abnormal in 4/10 patients [trisomy 19, t(5;6); inv(14) and inv(10)]. The study of Β-chain of the T-cell receptor revealed clonal rearrangements in 14 (78%), restricted to CD3+ patients (92%). In vitro culture of myeloid precursors showed decreased CFU-GM in 5/6 patients. Virological studies for HTLV-I and II were negative. In conclusion, the presence of a clonal proliferation was not correlated with the clinical course or an associated disease.Type of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesVillamor, N. ; Rozman, M. ; Esteve, J. ; Aymerich, M. ; Colomer, D. ; Aguilar, J. L. ; Campo, E. ; Montserrat, E.
Springer
Published 1999Staff ViewISSN: 1432-0584Keywords: Key words Anaplastic large-cell lymphoma ; leukemia ; ALK-1Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract Anaplastic large-cell lymphoma (ALCL) is a lymphoproliferative disorder that frequently presents with disseminated disease and extranodal involvement. Rare atypical cells have been detected in the peripheral blood in occasional cases. However, the presence of a prominent leukemic phase is extremely rare in these patients. We describe a patient with a small-cell variant of ALCL of T-cell phenotype, ALK-1 positive, who developed a rapid leukemic phase in association with the progression of the disease. Similar to the nodal biopsy, the predominant cells in bone marrow and peripheral blood were small atypical lymphoid cells. The large tumor cells expressed ALK immunoreactivity with a cytoplasmic and nuclear pattern, whereas some of the small cells showed only a nuclear-restricted pattern of staining. An RT-PCR study detected the NPM-ALK chimeric product in the nodal biopsy and in a peripheral blood sample in the early phase of the disease, but it became negative in a peripheral blood sample obtained after completion of the chemotherapy treatment, suggesting that this assay may be useful in the follow-up of these patients. This case indicates that a prominent leukemic phase may develop in ALCL as a manifestation of tumor dissemination and that it may be composed of a predominant small-cell atypical component.Type of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesRovira, A. ; Urbano-Ispizua, A. ; Cervantes, F. ; Rozman, M. ; Vives-Corrons, J. Ll. ; Montserrat, E. ; Rozman, C.
Springer
Published 1995Staff ViewISSN: 1432-0584Keywords: CML ; Blast crisis ; p53 gene ; Restriction fragment length polymorphismSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract Loss of the p53 gene alleles was investigated in 26 patients with Ph+BCR/ABL+ chronic myeloid leukemia (CML) by means of the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis using the restriction enzymeAccII. In all cases, peripheral blood and/or bone marrow samples were obtained at different times during the chronic phase of the disease and at blast crisis, and in some of them also at the accelerated phase. Of the 12 cases considered informative, 11 evolved into myeloid type blast crisis and one into a lymphoid blast crisis, whereas only two showed an i(17q) chromosome at cytogenetic study. In four of the 12 informative cases, a loss of one p53 gene allele was observed, in all cases coincident with the development of the accelerated phase or blast crisis. One patient with a deleted p53 gene allele, in whom it was possible to analyze the gene structure in the three CML evolutive phases (chronic and accelerated phases and blast crisis), showed loss of the p53 gene allele in both the accelerated and the biastic phase, but not during the chronic phase. On the other hand, one of the two cases with an i(17q) chromosome exhibited one allelic deletion of the p53 gene. Thus, the relatively frequent monoallelic deletion of the p53 gene coincident with the appearance of the blast crisis registered in the present study would support a possible role of the p53 gene alterations in the evolution of CML to its final stages.Type of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesRovira, A. ; Urbano-Ispizua, A. ; Cervantes, F. ; Rozman, M. ; Vives-Corrons, J. Ll. ; Montserrat, E. ; Rozman, C.
Springer
Published 1995Staff ViewISSN: 1432-0584Keywords: Key words CML ; Blast crisis ; p53 gene ; Restriction fragment length polymorphismSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract Loss of the p53 gene alleles was investigated in 26 patients with Ph+, BCR/ABL+ chronic myeloid leukemia (CML) by means of the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) analysis using the restriction enzyme AccII. In all cases, peripheral blood and/or bone marrow samples were obtained at different times during the chronic phase of the disease and at blast crisis, and in some of them also at the accelerated phase. Of the 12 cases considered informative, 11 evolved into myeloid type blast crisis and one into a lymphoid blast crisis, whereas only two showed an i(17q) chromosome at cytogenetic study. In four of the 12 informative cases, a loss of one p53 gene allele was observed, in all cases coincident with the development of the accelerated phase or blast crisis. One patient with a deleted p53 gene allele, in whom it was possible to analyze the gene structure in the three CML evolutive phases (chronic and accelerated phases and blast crisis), showed loss of the p53 gene allele in both the accelerated and the blastic phase, but not during the chronic phase. On the other hand, one of the two cases with an i(17q) chromosome exhibited one allelic deletion of the p53 gene. Thus, the relatively frequent monoallelic deletion of the p53 gene coincident with the appearance of the blast crisis registered in the present study would support a possible role of the p53 gene alterations in the evolution of CML to its final stages.Type of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesMassad, E. ; Rozman, M. ; Azevedo, R.S. ; Silveira, A.S.B. ; Takey, K. ; Yamamoto, Y.I. ; Strazza, L. ; Ferreira, M.M.C. ; Carvalho, H.B. ; Burattini, M.N.
Springer
Published 1999Staff ViewISSN: 1573-7284Keywords: AIDS ; Hepatitis C ; Injecting drug abusers ; Prisoners ; SeroprevalenceSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract Between November 1993 and April 1994, our physicians' team interviewed and took blood samples of 631 prisoners randomly drawn from the largest prison of South America, which counted about 4700 inmates at that time. The interview consisted of questions related to risk behaviour for HIV infection, and the subjects were asked to provide blood for serological tests for HIV, hepatitis C and syphilis. Our main purpose was to investigate the relationship between HCV and injecting drug use as related to HIV seropositivity. Participation in the study was voluntary and confidentiality was guaranteed. Overall prevalences found were as follows: HIV: 16% (95% confidence interval (CI): 13–19%); HCV: 34% (95% CI: 30–38%), and syphilis: 18% (95% CI: 15–21%). Acknowledged use of ever injecting drug was 22% and no other parenteral risk was reported. Our results, as compared with other studies in the same prison, suggest that HIV prevalence has been stable in recent years, and that the major risk factor for HIV infection in this population is parenteral exposure by injecting drug use.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesStaff View
ISSN: 1573-4803Source: Springer Online Journal Archives 1860-2000Topics: Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision MechanicsNotes: Abstract High T c superconducting materials based on a PbO-modified Bi-Sr-Ca-Cu-O system with various ratios between the oxides were prepared by calcination at 800 °C and firing at 855 °C. From X-ray powder diffraction analysis data, the ratio of low- and high-temperature phases was calculated. The material with the nominal composition Bi2Pb0.5Sr2Ca2.5Cu3.5O x was chosen for further experimental work. Samples fired at 800 °C contain mostly the low-temperature phase (2212). Higher firing temperatures lead to the formation of the high T c phase (2223) with T c(R=0) over 100 K. Some samples were cold pressed and refired which increased the specific density to over 80% of the theoretical density. The composition of samples was investigated by X-ray powder diffraction analysis and by energy dispersive X-ray spectroscopy. The main phase in the material fired at 800 °C, is the low T c phase 2212, and secondary phases are Ca2PbO4, unreacted CuO and traces of 2223 phase. At higher firing temperatures, the main phase is the high-temperature phase 2223. The material is still heterogeneous and contains Ca2PbO4.Type of Medium: Electronic ResourceURL: