Search Results - (Author, Cooperation:M. Quinones)

2018-06-19

American Physical Society (APS)

1539-3755

1550-2376

Physics

Biological Physics

2014-12-30

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Antigens, Bacterial/immunology ; CD8-Positive T-Lymphocytes/cytology/*immunology ; Dendritic Cells/cytology/*immunology ; Humans ; Immunity, Innate/immunology ; Interleukin-17/immunology ; Langerhans Cells/cytology/immunology ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Primates ; Skin/cytology/*immunology/*microbiology ; Staphylococcus epidermidis/immunology ; Symbiosis/*immunology

2012-07-28

American Association for the Advancement of Science (AAAS)

0036-8075

1095-9203

Biology

Chemistry and Pharmacology

Computer Science

Medicine

Natural Sciences in General

Physics

Animals ; Host-Pathogen Interactions ; Humans ; Immunity ; Intestines/immunology/microbiology/pathology ; Metagenome/*immunology ; Mice ; Skin/*immunology/*microbiology ; Skin Diseases, Bacterial/*immunology/pathology ; T-Lymphocytes/*immunology

1439-0523

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Four tomato lines introgressed from Lycopersicon chilense were compared with the commercial F1 hybrids ‘ARO 8479’ and ‘HA 3108’, which are tolerant to Tomato yellow leaf curl virus, and the cv. ‘Campbell 28’ as a susceptible control. Resistance was evaluated by the use of grafted diseased scions as well as in a field trial where plants infected by viruliferous whiteflies and disease-free plants were transplanted in paired rows. The new lines LD 3, LD 4, LD 5 and LD 6 showed no disease symptoms after grafting or in the field trial. Virus accumulation at 60 days after transplanting was low in the infected plants: 0.09, 0.60, 1.00 and 0.50 ng, respectively. No fruit-set or yield losses were registered under the high temperature conditions prevalent in the trial, in which lines LD 5 and LD 6 were better adapted to tropical conditions. Viral DNA concentrations were over 1000 ng in the cvs.‘Campbell 28′,‘ARO 8479’ and ‘HA 3108’. The last two are considered tolerant as they were asymptomatic or had mild symptoms, respectively, but achieved acceptable yields in the trial. By contrast, virus had a negative effect on fruit-set, number of fruit per plant and total yield in the cv.‘Campbell 28’.

Electronic Resource

1365-3040

Blackwell Publishing Journal Backfiles 1879-2005

Biology

In M. braunii, the uptake of NO3− and NO2− is blue-light-dependent and is associated with alkalinization of the medium. In unbuffered cell suspensions irradiated with red light under a CO2-free atmosphere, the pH started to rise 10s after the exposure to blue light. When the cellular NO3− and NO2− reductases were active, the pH increased to values of around 10, since the NH4+ generated was released to the medium. When the blue light was switched off, the pH stopped increasing within 60 to 90s and remained unchanged under background red illumination. Titration with H2SO4 of NO3− or NO2− uptake and reduction showed that two protons were consumed for every one NH4+ released. The uptake of Cl− was also triggered by blue light with a similar 10 s time response. However, the Cl− -dependent alkalinization ceased after about 3 min of blue light irradiation. When the blue light was turned off, the pH immediately (15 to 30 s) started to decline to the pre-adjusted value, indicating that the protons (and presumably the Cl−) taken up by the cells were released to the medium. When the cells lacked NO3− and NO2− reductases, the shape of the alkalinization traces in the presence of NO3− and NO2− was similar to that in the presence of Cl−, suggesting that NO3− or NO2− was also released to the medium. Both the NO3− and Cl−-dependent rates of alkalinization were independent of mono- and divalent cations.

Electronic Resource

1365-3040

Blackwell Publishing Journal Backfiles 1879-2005

Biology

Sensitivity to light quality and pigment composition were analysed and compared in abaxial and adaxial stomata of Gossypium barbadense L. (Pima cotton). In most plants, abaxial (lower) stomatal conductances are higher than adaxial (upper) ones, and stomatal opening is more sensitive to blue light than to red. In greenhouse-grown Pima cotton, abaxial stomatal conductances were two to three times higher than adaxial ones. In contrast, adaxial stomatal conductances were 1·5 to two times higher than abaxial ones in leaves from growth chamber-grown plants. To establish whether light quality was a factor in the regulation of the relationship between abaxial and adaxial stomatal conductances, growth-chamber-grown plants were exposed to solar radiation outdoors and to increased red light in the growth chamber. In both cases, the ratios of adaxial to abaxial stomatal conductance reverted to those typical of greenhouse plants. We investigated the hypothesis that adaxial stomata are more sensitive to blue light and abaxial stomata are more sensitive to red light. Measurements of stomatal apertures in mechanically isolated epidermal peels from growth chamber and greenhouse plants showed that adaxial stomata opened more under blue light than under red light, while abaxial stomata had the opposite response. Using HPLC, we quantified the chlorophylls and carotenoids extracted from isolated adaxial and abaxial guard cells. All pigments analysed were more abundant in the adaxial than in the abaxial guard cells. Antheraxanthin and β-carotene contents were 2·3 times higher in adaxial than in abaxial guard cells, comparing with ad/ab ratios of 1·5–1·9 for the other pigments. We conclude that adaxial and abaxial stomata from Pima cotton have a differential sensitivity to light quality and their distinct responses are correlated with different pigment content.

Electronic Resource

0038-1098

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Physics

Electronic Resource

1398-9995

Blackwell Publishing Journal Backfiles 1879-2005

Medicine

Electronic Resource

1432-8798

Springer Online Journal Archives 1860-2000

Medicine

Summary.  The cloning and nucleotide sequence of a new bipartite geminivirus found in Cuba is described. DNA A (2620 nt) and DNA B (2586 nt) presented a genomic structure resembling that of other geminiviruses transmitted by Bemisia tabaci. Both components had a common region of 168 nt with a 95% identity. Typical elements involved in replication and transcription were found in this region, though group-characteristic arrangement of iterons was not conserved in this virus. Sequence was compared with geminivirus sequences deposited in the GenBank. Interestingly, when total DNAs or individual ORFs and deduced amino acid sequences were compared, the highest scores were for different viruses. It showed to be most closely related to tomato mottle virus (81.9% and 65.5% similarity with DNAs A and B, respectively) and a member of the abutilon mosaic cluster of New World Begomoviruses. When clones A and B were co-agroinoculated they resulted highly infectious and induced symptoms in Nicotiana benthamiana plants. The A component alone was infectious but induced only mild symptoms, while the B component was not infectious. The presence of viral DNA in N. benthamiana plants was confirmed by dot-blot hybridization using specific probes. These data show that the cuban isolate is a new geminivirus for which the name of Havana tomato virus is proposed.

Electronic Resource

1434-9957

Natural language processing ; Natural language interfaces ; Speech interfaces ; Speech interface design ; Speech recognition ; Virtual reality

Springer Online Journal Archives 1860-2000

Computer Science

Abstract Two research projects are described that explore the use of spoken natural language interfaces to virtual reality (VR) systems. Both projects combine off-the-shelf speech recognition and synthesis technology with in-house command interpreters that interface to the VR applications. Details about the interpreters and other technical aspects of the projects are provided, together with a discussion of some of the design decisions involved in the creation of speech interfaces. Questions and issues raised by the projects are presented as inspiration for future work. These issues include: requirements for object and information representation in VR models to support natural language interfaces; use of the visual context to establish the interaction context; difficulties with referencing events in the virtual world; and problems related to the usability of speech and natural language interfaces in general.

Electronic Resource