Search Results - (Author, Cooperation:M. Navajas)
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1Latest Papers from Table of Contents or Articles in PressM. Grbic ; T. Van Leeuwen ; R. M. Clark ; S. Rombauts ; P. Rouze ; V. Grbic ; E. J. Osborne ; W. Dermauw ; P. C. Ngoc ; F. Ortego ; P. Hernandez-Crespo ; I. Diaz ; M. Martinez ; M. Navajas ; E. Sucena ; S. Magalhaes ; L. Nagy ; R. M. Pace ; S. Djuranovic ; G. Smagghe ; M. Iga ; O. Christiaens ; J. A. Veenstra ; J. Ewer ; R. M. Villalobos ; J. L. Hutter ; S. D. Hudson ; M. Velez ; S. V. Yi ; J. Zeng ; A. Pires-daSilva ; F. Roch ; M. Cazaux ; M. Navarro ; V. Zhurov ; G. Acevedo ; A. Bjelica ; J. A. Fawcett ; E. Bonnet ; C. Martens ; G. Baele ; L. Wissler ; A. Sanchez-Rodriguez ; L. Tirry ; C. Blais ; K. Demeestere ; S. R. Henz ; T. R. Gregory ; J. Mathieu ; L. Verdon ; L. Farinelli ; J. Schmutz ; E. Lindquist ; R. Feyereisen ; Y. Van de Peer
Nature Publishing Group (NPG)
Published 2011Staff ViewPublication Date: 2011-11-25Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: Adaptation, Physiological/*genetics/physiology ; Animals ; Ecdysterone/analogs & derivatives/genetics ; Evolution, Molecular ; Fibroins/genetics ; Gene Expression Regulation ; Gene Transfer, Horizontal/genetics ; Genes, Homeobox/genetics ; Genome/*genetics ; Genomics ; Herbivory/*genetics/physiology ; Molecular Sequence Data ; Molting/genetics ; Multigene Family/genetics ; Nanostructures/chemistry ; Plants/parasitology ; Silk/biosynthesis/chemistry ; Tetranychidae/*genetics/*physiology ; Transcriptome/geneticsPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 1572-9702Keywords: nucleotide sequences variation ; ribosomal DNA ; ITS ; PhytoseiidaeSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Preliminary work is needed to assess the usefulness of different markers at different taxonomic scales when a new group is analyzed, such as the commercially important Phytoseiidae mites. We investigate here the level of sequence variation of the nuclear ribosomal spacers ITS 1 and 2 and the 5.8S gene in six species of Phytoseiidae: Neoseiulus californicus, N. fallacis, Euseius concordis, Metaseiulus occidentalis, Typhlodromus pyri and Phytoseiulus persimilis. As expected, the 5.8S gene (148 base pairs) is markedly conserved and displays little variation in between genera comparisons. ITS1 and ITS2 show contrasting patterns: while the ITS2 is short (80–89 bp) and shows little variation, the ITS1 is longer (303–404 bp) and is very variable in sequence. This fact compromises reliable nucleotide homologies when comparing the genera. The comparison of ITS1 sequence similarity at the species level might be useful for species identification, however, the value of ITS in taxonomic studies does not extend to the level of the family. The intraspecific variations of ITS were investigated in three species: N. californicus, N. fallacis and E. concordis. The first species has identical ITS1 sequences and the last two display low polymorphism (2 nucleotide substitutions). The ITS2 and 5.8S sequences were identical in all three subspecies comparisons.Type of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1572-9702Source: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract DNA sequence data were used to examine phylogenetic relationships between six species of economically important Tetranychidae mites:Eotetranychus carpini (Oudemans),E. pruni (Reck),Tetranychus pacificus McGregor,T. mcdanieli McGregor,T. turkestani Ugarov & Nikolski andT. urticae Koch. With primers directed toward conserved elements flanking the target region, the Polymerase Chain Reaction was used to amplify the ITS2 spacer of the ribosomal DNA molecule. The nucleotide sequence of a 300-bp fragment of the ITS2 was determined by direct sequencing and nucleotide divergence used for intra-generic comparison in mites. The resulting phylogenetic tree expressing interspecific relationships in genusTetranychus agrees with morphological data. The study demonstrates the usefulness of the approach in the assessment of the systematics and evolution of the group.Type of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesNavajas, M. ; Gutierrez, J. ; Bonato, O. ; Bolland, H. R. ; Mapangou-Divassa, S.
Springer
Published 1994Staff ViewISSN: 1572-9702Keywords: Mononychellus progresivus ; biodiversity ; molecular biology ; genetics ; cassavaSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract Intraspecific diversity in Cassava Green Mite Mononychellus progresivus Doreste was examined using individuals collected in Benin and the Congo and in Columbia and Brazil. Comparisons were based on mitochondrial and ribosomal DNA sequences and the results of several cross-breeding experiments. Sequence variation was examined in a total of 1139 base pairs (bp) constituting the ITS2 ribosomal DNA (805 bp) and a fragment of the Cytochrome Oxidase I (COI) gene (334 bp). Sequence divergence is low, ranging from 0% to 2.1% for COI and from 0% to 0.4% for ITS2. Inter-strain comparisons have shown that the two African populations appear to be identical. They were similar to the Colombian population while the Brazilian population was clearly different. The data support the hypothesis of a single introduction of the species in the two African populations. Crossing experiments have shown partial hybrid sterility, suggesting a genetic incompatibility consistent with differences detected by sequence data. The results show the usefulness of molecular markers as a tool for determining taxonomic status and dispersion paths in spider mites.Type of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 1572-9702Keywords: AFLP ; allozymes ; DALP ; DNA sequencing ; genetic structure ; microsatellites ; mitochondrial DNA ; mites ; molecular systematics ; PCR ; phylogeny ; RAPD ; RFLP ; ribosomal DNA ; ticksSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Abstract The application of molecular markers to the study of ticks and mites has recently yielded new insights into their population structures and taxonomic relationships. Ticks have been studied at individual, population and species level. Mites are a more diverse group and those that have been studied to the same degree as the ticks include the Tetranychidae (spider mites), Phytoseiidae (predatory mites) and the Eriophyidae. Population variation has also been studied in the important bee parasitic mite Varroa jacobsoni Oudemans. The methods used to study these organisms have much in common. At the individual level these range from general approaches, such as AFLP, RAPD or DALP, to highly specific microsatellite analysis. Although these markers also work at the population and species level, additional analysis of specific nuclear or mitochondrial genes has been conducted either by RFLP or sequencing. Molecular applications have had particular success in facilitating the identification of taxonomically difficult species, understanding population structures and elucidating phylogenetic relationships.Type of Medium: Electronic ResourceURL: