Search Results - (Author, Cooperation:M. Manzanares)

2014-03-22

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Adipose Tissue/metabolism ; Animals ; Basal Metabolism/genetics ; Body Mass Index ; Body Weight/genetics ; Brain/metabolism ; Diabetes Mellitus, Type 2/genetics ; Diet ; Genes, Dominant/genetics ; Homeodomain Proteins/*genetics/metabolism ; Humans ; Hypothalamus/metabolism ; Introns/*genetics ; Male ; Mice ; Mixed Function Oxygenases/*genetics ; Obesity/*genetics ; Oxo-Acid-Lyases/*genetics ; Phenotype ; Polymorphism, Single Nucleotide/genetics ; Promoter Regions, Genetic/genetics ; Proteins/*genetics ; Thinness/genetics ; Transcription Factors/deficiency/*genetics/metabolism ; Zebrafish/embryology/genetics

2013-09-13

Nature Publishing Group (NPG)

0028-0836

1476-4687

Biology

Chemistry and Pharmacology

Medicine

Natural Sciences in General

Physics

Animals ; Blood Cells/cytology/metabolism ; Cell Dedifferentiation ; Cell Separation ; Cells, Cultured ; *Cellular Reprogramming/genetics ; Ectoderm/cytology ; Embryoid Bodies/cytology/metabolism ; Embryonic Stem Cells/cytology/metabolism ; Female ; Fibroblasts/cytology ; Gene Expression Profiling ; Induced Pluripotent Stem Cells/*cytology/metabolism ; Intestines/cytology ; Kidney/cytology ; Kruppel-Like Transcription Factors/genetics/metabolism ; Male ; Mice ; Mice, Inbred C57BL ; Octamer Transcription Factor-3/genetics/metabolism ; Organ Specificity ; Pancreas/cytology ; Proto-Oncogene Proteins c-myc/genetics/metabolism ; SOXB1 Transcription Factors/genetics/metabolism ; Stomach/cytology ; Teratoma/genetics/*metabolism/pathology ; Totipotent Stem Cells/*cytology/metabolism ; Transcriptome/genetics ; Trophoblasts/cytology

1365-3059

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

The effects of single and mixed artificial infections by artichoke latent virus (ALV) and broad bean wilt virus isolate FA (BBWV-FA) on components of growth and yield of three globe artichoke lines were evaluated in an aphid-free glasshouse. Virus infections reduced plant vigour (length of leaves and height of the ramifications) and dry weight, increased dry matter percentage, lengthened harvest period, and induced significant losses in the number of harvested buds, which resulted from reduced production of ramifications. However, the magnitude of affected parameters depended on inherent line differences and the type of virus infection. The greatest reductions occurred with the ALV + BBWV-FA infection. In single infections ALV led to the more severe effects, especially on components of yield. The level of ALV coat protein, as determined by ELISA, indicated differences in titre between plants of three lines. Can line, which exhibited greater yield reduction than Cada or CBT lines, had the higher ALV virus titre. Conversely Cari was less susceptible to BBWV-FA infection than Cada. The detrimental effects of virus infection emphasize the need for virus control, and differences in responses among lines indicate the possibility of control by plant breeding.

Electronic Resource

1365-3059

Blackwell Publishing Journal Backfiles 1879-2005

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Variation for virulence was examined amongst 20 field collections of Plasmodiophora brassicae from France. Out of the 10 brassica lines tested, seven reacted differentially to inoculation; of these, two oilseed rape cultivars exhibited previously unreported differential responses. Some of the differential lines used previously to classify pathotypes of P. brassicae were susceptible to all collections, suggesting that pathogen populations in France may be different from those reported elsewhere. Good pathotype discrimination was obtained using a set of three cultivars of Brassica napus. Five pathotypes, P1, P2, P3, P4 and P5, were detected and their occurrence was unrelated to host type. Pathogenic variation amongst 17 single-spore isolates derived from three field collections was studied, and five pathotypes were identified. Four isolates were classified as pathotype P1, pathogenic on all three differential hosts, and eight as pathotype P4, pathogenic on none of the three differentials. The five other isolates were classified as pathotypes P3, P6 and P7, the latter two expressing patterns of reaction not observed for field collections. The fractionation of different individual pathotypes from one original spore suspension confirmed the genetic heterogeneity of field populations of P. brassicae.

Electronic Resource

0273-1177

Elsevier Journal Backfiles on ScienceDirect 1907 - 2002

Mechanical Engineering, Materials Science, Production Engineering, Mining and Metallurgy, Traffic Engineering, Precision Mechanics

Physics

Electronic Resource

1432-0800

Springer Online Journal Archives 1860-2000

Energy, Environment Protection, Nuclear Power Engineering

Medicine

Electronic Resource

1432-0568

Chondroid tissue ; Skull development ; Human growth ; Microradiography

Springer Online Journal Archives 1860-2000

Medicine

Summary The cranial vault of fifteen human subjects varying in age from 20th week of gestational life to 9th month post-matum were submitted to microradiographic and histological analysis. Different phenomena such as cortical drift, bone cavitation and progressive substitution of different calcified tissues by lamellar bone are illustrated. Moreover, this study reveals in several areas the presence of chondroid tissue; it constitutes the edges of the sutures and is responsible for their growth till the post-natal period. Therefore, it can be supported that the role of chondroid tissue is essential for the harmonious development of the cranial vault.

Electronic Resource

1432-0584

Bone marrow transplantation ; Acute leukemia ; CFU-GM ; Granulocyte compartment

Springer Online Journal Archives 1860-2000

Medicine

Summary The possible interrelation between infused bone marrow CFU-GM and peripheral granulocyte recovery was studied in 16 patients transplanted for acute leukemia. The influence of several clinical events that could modify the graft fate were also analysed. Our results show that: 1) There was no correlation between the number of infused nucleated cells and granulocyte recovery. 2) There was no correlation between the number of infused CFU-GM and granulocyte regeneration. 3) There were significant differences between the day of engraftment in patients with clinically documented HSV infection compared to patients without infection.

Electronic Resource

1432-041X

Artemia ; Crustacea ; engrailed ; Parasegment ; Segmentation

Springer Online Journal Archives 1860-2000

Biology

Abstract We have studied the process of post-embryonic segmentation in the anostracan crustacean Artemia franciscana using a specific antibody raised against the engrailed protein of this organism. Three cephalic segments are specified during embryonic development, before larval hatching, whilst trunk (thoracic) segmentation begins after the first stage free-swimming nauplius larva has emerged from the dormant cyst. Thus, cephalic and trunk segmentation seem to be at least in part independent and superimposed processes. Trunk stripes of engrailed expression are added one at a time as segments are generated from the posterior growth zone. The first detectable decision in engrailed expression is the establishment of a line of engrailed-expressing cells, interpretable as delineating the parasegmental boundary. The subsequent widening of engrailed stripes is not correlated with cell lineage events but is probably mediated by the combination of inheritance of the active state and recruitment of new cells into the engrailed-expressing stripe.

Electronic Resource

1432-119X

Springer Online Journal Archives 1860-2000

Biology

Medicine

Abstract Performing the antibody reaction under controlled heating through microwave irradiation results in significant improvements in the immunovisualization of antigens, such as shortening the times of incubation, lowering the antibody titres used and allowing the detection of difficult, inaccessible antigens. In addition to investigate the basis of the enhancement, we have extended to more intact samples such as isolated plant nuclei and in toto Drosophila embryos the results previously reported only with tissue sections. A drop of heterologous anti-nucleolin antibody covering isolated nuclei from onion root meristems spread on a glass slide was microwave irradiated, resulting in clear immunofluorescent labelling of the nucleoli. This result was never previously obtained in the absence of microwave treatment, even using the complicated procedure previously reported for the homologous identification of this nuclear protein. Using the much larger and to some extent impermeable Drosophila embryos, we were able to show that the incubation time and concentration of the anti-myosin antibody can be strongly reduced by performing the reaction at 45° C under microwave irradiation. The controlled increase in temperature is the main factor responsible for these improvements; the importance of maintaining an adequate mixing of the samples is also emphasized. The proper implementation of these two experimental conditions will require the introduction of appropriate mixing accessories and temperature measuring probes for samples of small volume in current microwave laboratory ovens.

Electronic Resource

1573-5036

Fragaria vesca ; iron-chelates ; iron nutritional indexes ; uptake

Springer Online Journal Archives 1860-2000

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Abstract Several indexes are used to determine the iron nutritional status of plants, but their effectiveness depends either on the plant growth conditions in natural environments or on the assay conditions. This research was conducted to test different indexes of the iron nutritional status of a hydroponic strawberry culture where treatments mainly differed in the source of the iron applied: Fe-EDTA, Fe-EDDHA and Fe-polyflavonoid. Macro and micronutrient concentrations in the nutrient solutions, leaf and vascular tissues were measured. Fe concentration in the nutrient solution during the course of the experiment was considered in relation to the stability of the different chelates. Both Fe concentration and total Fe content of leaves reflected the effect of the treatments; Fe/Mn ratio was significant as a diagnosis index. Other element ratios as P/Fe and K/Ca are not well related with the iron nutrition symptoms observed. Fe2+ concentration measured in leaves was not directly affected by the different chelate treatments.

Electronic Resource

1572-9680

adventitious rooting ; bladder senna ; IBA ; leafless hardwood stem cuttings ; shrubby legumes

Springer Online Journal Archives 1860-2000

Agriculture, Forestry, Horticulture, Fishery, Domestic Science, Nutrition

Abstract The vegetative propagation of the multipurpose leguminous shrub Colutea arborescens from leafless hardwood cuttings was examined. Specific attention was paid to the factors: 1) cutting origin (apical, medial or basal); 2) indole-3-butyric acid (IBA) treatment (0, 50 or 200 ppm); and 3) relative humidity of the propagation environment (high or low humidity). After 120 days, 67.6% of the C. arborescens cuttings had rooted. The rooting ability of the cuttings was significantly influenced by the cutting origin and hormonal treatment. No effects on rooting percentage were shown by the relative humidity of the propagation environment. The best rooting response (95%) was shown by basal cuttings treated with 200 ppm IBA. Cutting origin, IBA treatment and relative humidity were significantly related to the root number, longest root length and the fresh/dry biomass of roots and leaves. Under conditions of high humidity, basal cuttings treated with 200 ppm IBA gave rise to the most highly developed plants. The positive relationship between sprouting and rooting suggests that successful propagation is related to the shoot formation potential of the cutting's food reserves. These findings were used to propose an efficient method for the vegetative propagation of C. arborescens plants.

Electronic Resource