Search Results - (Author, Cooperation:M. Brendel)
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1Latest Papers from Table of Contents or Articles in PressC. Timmann ; T. Thye ; M. Vens ; J. Evans ; J. May ; C. Ehmen ; J. Sievertsen ; B. Muntau ; G. Ruge ; W. Loag ; D. Ansong ; S. Antwi ; E. Asafo-Adjei ; S. B. Nguah ; K. O. Kwakye ; A. O. Akoto ; J. Sylverken ; M. Brendel ; K. Schuldt ; C. Loley ; A. Franke ; C. G. Meyer ; T. Agbenyega ; A. Ziegler ; R. D. Horstmann
Nature Publishing Group (NPG)
Published 2012Staff ViewPublication Date: 2012-08-17Publisher: Nature Publishing Group (NPG)Print ISSN: 0028-0836Electronic ISSN: 1476-4687Topics: BiologyChemistry and PharmacologyMedicineNatural Sciences in GeneralPhysicsKeywords: ABO Blood-Group System ; Anemia, Sickle Cell ; Case-Control Studies ; Chromosomes, Human, Pair 1/genetics ; Chromosomes, Human, Pair 16/genetics ; Disease Resistance/*genetics ; Genetic Loci/*genetics ; *Genome-Wide Association Study ; Ghana ; Humans ; Malaria, Falciparum/*genetics/parasitology/pathology ; Membrane Proteins/genetics ; Plasma Membrane Calcium-Transporting ATPases/genetics ; Polymorphism, Single Nucleotide/geneticsPublished by: -
2Articles: DFG German National LicensesStaff View
ISSN: 1011-1344Keywords: DNA adducts ; Psoralen ; Saccharomyces cerevisiae. ; molecular dosimetry ; mutagenesisSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicineType of Medium: Electronic ResourceURL: -
3Articles: DFG German National LicensesStaff View
ISSN: 1011-1344Keywords: DNA adducts ; Psoralen ; Saccharomyces cerevisiae. ; gel chromatography ; hydroxylapatite chromatography ; molecular dosimetrySource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyChemistry and PharmacologyMedicineType of Medium: Electronic ResourceURL: -
4Articles: DFG German National LicensesStaff View
ISSN: 0921-8777Keywords: DNA repair ; Gap repair ; Thermoconditional mutantSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
5Articles: DFG German National LicensesStaff View
ISSN: 0027-5107Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
6Articles: DFG German National LicensesStaff View
ISSN: 0027-5107Keywords: Phosphate transport ; Polyphosphate breakdown ; Sacchamyces cerevisiae ; Thymidylate mutagenesisSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
7Articles: DFG German National LicensesStaff View
ISSN: 0027-5107Keywords: DNA strand breaks ; Saccharomyces cerevisiae ; Thymidylate excessSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
8Articles: DFG German National LicensesStaff View
ISSN: 0027-5107Keywords: DNA ; repair and mutagenesisSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
9Articles: DFG German National LicensesStaff View
ISSN: 0027-5107Keywords: Formaldehyde resistance ; Plasmid stability ; RecombinationSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
10Articles: DFG German National LicensesStaff View
ISSN: 0921-8777Keywords: Choline permease ; DNA repair ; Nitrogen mustardSource: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
11Articles: DFG German National LicensesStaff View
ISSN: 1436-5081Source: Springer Online Journal Archives 1860-2000Topics: MathematicsType of Medium: Electronic ResourceURL: -
12Articles: DFG German National LicensesMahler, R. ; Franke, F. E. ; Hering, B. J. ; Brandhorst, D. ; Brandhorst, H. ; Brendel, M. D. ; Federlin, K. ; Schulz, A. ; Bretzel, R. G.
Springer
Published 1999Staff ViewISSN: 1432-1440Keywords: Key Words Pancreas ; Islets of Langerhans ; Islet isolation ; Pancreas morphologySource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Abstract In clinical islet transplantation to patients with type 1 diabetes mellitus, the number of isolated and purified islet has been identified as a key determinant for functional success of the islet graft [1]. With improved isolation methods based on the original procedure published by Ricordi et al. [2] yield and function of isolated islets were considerably enhanced. However, there is still a large variance in the number, purity, viability and secretory capacity of islets isolated from brain-dead human donor pancreata, significantly hampering utilization of human islet preparations derived from a single donor for one diabetic recipient. The reasons for the limited success in islet isolation and purification have not been clarified in detail yet. Recent studies have indicated, that donor preconditions, and a number of technical factors during organ procurement and the islet isolation process itself are critical to successful islet isolation [3, 4]. This study aimed at identifying distinct morphological and histopathological characteristics of the donor pancreas as determinants for the outcome of human islet isolation and purification.Type of Medium: Electronic ResourceURL: -
13Articles: DFG German National LicensesBretzel, R. G. ; Brandhorst, D. ; Brandhorst, H. ; Eckhard, M. ; Ernst, W. ; Friemann, S. ; Rau, W. ; Weimar, B. ; Rauber, K. ; Hering, B. J. ; Brendel, M. D.
Springer
Published 1999Staff ViewISSN: 1432-1440Keywords: Key words Islet isolation ; Clinical islet transplantation ; Type-1 diabetes mellitus ; ImmunosuppressionSource: Springer Online Journal Archives 1860-2000Topics: MedicineType of Medium: Electronic ResourceURL: -
14Articles: DFG German National LicensesStaff View
ISSN: 1432-0983Keywords: Saccharomyces cerevisiae ; Psoralen photoaddition ; Interstrand cross-link ; Repair deficiency ; GenotoxicitySource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: Summary Two different UVA irradiation systems were initially biologically calibrated with two haploid yeast strains proficient and deficient, respectively, in nucleotide excision repair. The number of DNA lesions introduced into the cell's genome by the photoactivated bifunctional furocoumarin 8-MOP was then calculated by means of the applied UVA exposure doses. At LD37 the repair-proficient wild type had about 14 ICL and 34 furan-side monoadducts in its DNA, while doubly blocked repair mutant rad3-12 pso1-1 had 2 ICL and 3 monoadducts. Locus-specific reversion of lys1-1 followed two-hit kinetics in the repair-proficient wild type and one-hit kinetics in an excision-deficient rad2-20 mutant, as would be expected if ICL was the main type of mutagenic lesion in the wild type and monoadducts the main mutagenic lesion type in the excision-deficient strain. Quantitative comparison of 8-MOP + UVA-induced ICL with those induced by bifunctional mustard revealed the former to have a much higher genotoxicity.Type of Medium: Electronic ResourceURL: -
15Articles: DFG German National LicensesStaff View
ISSN: 1432-0983Keywords: Mutagen resistance ; DNA sequencing ; Transmembrane protein ; Gene disruptionSource: Springer Online Journal Archives 1860-2000Topics: BiologyNotes: LEU2 disruption mutants, with sublethal doses of aminotriazole induced hyper-resistance to 4-nitroquinoline-N-oxide. Partial deletion of the nucleotide sequence coding for a putative ATP-binding site has no, or little, influence on resistance to 4NQO whereas total deletion of the region coding for this ATP-binding domain leads to 4NQO-sensitive nullmutants.Type of Medium: Electronic ResourceURL: -
16Articles: DFG German National LicensesStaff View
ISSN: 0165-7992Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
17Articles: DFG German National Licensesvan Zeeland, A.A. ; Mohn, G.R. ; Aaron, C.S. ; Glickman, B.W. ; Brendel, M. ; de Serres, F.J. ; Hung, C.Y. ; Brockman, H.E.
Amsterdam : ElsevierStaff ViewISSN: 0165-7992Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
18Articles: DFG German National LicensesStaff View
ISSN: 0165-1161Source: Elsevier Journal Backfiles on ScienceDirect 1907 - 2002Topics: BiologyMedicineType of Medium: Electronic ResourceURL: -
19Articles: DFG German National LicensesZumsteg, U. ; Reimers, J. I. ; Pociot, F. ; Mørch, L. ; Helqvist, S. ; Brendel, M. ; Alejandro, R. ; Mandrup-Poulsen, T. ; Dinarello, C. A. ; Nerup, J.
Springer
Published 1993Staff ViewISSN: 1432-0428Keywords: Cytokine ; interleukin-1 receptor antagonist ; insulin-dependent diabetes mellitusSource: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary The monokines interleukin-1α and -β have been implicated as effector molecules in the immune-mediated pancreatic beta-cell destruction leading to insulin-dependent diabetes mellitus. Here we investigated the effects of interleukin-1 receptor antagonism on insulin and glucagon release of rat, mouse and human islets exposed to recombinant human interleukin-1β, and on interleukin-1β induced changes in blood glucose, serum insulin and serum glucagon levels in Wistar Kyoto rats. The interleukin-1 receptor antagonist reduced the co-mitogenic effect of interleukin-1β on mouse and rat thymocytes with a 50% inhibitory concentration of 10- and 100-fold molar excess, respectively. Complete inhibition was obtained with a 100–1,000-fold molar excess. However, at a 100-fold molar excess the interleukin-1 receptor antagonist did not antagonise the potentiating effect of interleukin-1βon rat islet insulin accumulation during 3 and 6 h of exposure or of interleukin-1β-induced inhibition of insulin release after 24 h. In contrast, interleukin-1β-stimulated islet glucagon release was completely antagonised by a 100-fold molar excess of interleukin-1 receptor antagonist. A 10,000-fold molar excess of interleukin-1 receptor antagonist was needed to antagonise interleukin-1β stimulatory and inhibitory effects on rat beta-cell function in vitro. A 100-fold excess of interleukin-1 receptor antagonist could not counteract interleukin-1β effects on mouse and human beta cells, excluding species difference in the efficacy of the human interleukin-1 receptor antagonist. An anti-mouse interleukin-1 receptor type I antibody completely abolished interleukin-1β effects on isolated mouse islets. A 10–100-fold molar excess of interleukin-1 receptor antagonist antagonised interleukin-1β-induced fever, hypercorticosteronaemia and hyperglucagonaemia, but not interleukin-1β-induced reduction in insulin/glucose ratio in normal rats. In conclusion, our results suggest that antagonism of interleukin-1β effects on beta cells requires higher concentrations of interleukin-1 receptor antagonist than those necessary to block interleukin-1 action on islet alpha cells and other interleukin-1 targets in vitro and in vivo. This may contribute to the understanding of the specificity of the immunological beta-cell destruction leading to insulin-dependent diabetes.Type of Medium: Electronic ResourceURL: -
20Articles: DFG German National LicensesJu, Q. ; Edelstein, D. ; Brendel, M. D. ; Brandhorst, D. ; Brandhorst, H. ; Bretzel, R. G. ; Brownlee, M.
Springer
Published 1998Staff ViewISSN: 1432-0428Keywords: Keywords Lentiviral vector ; retrovirus ; human islet beta-cell ; gene transfer ; transplantation.Source: Springer Online Journal Archives 1860-2000Topics: MedicineNotes: Summary Pancreatic islet cells are terminally differentiated endocrine cells and are refractory to stable infection by retroviral vectors, which require the breakdown of the nuclear membrane during cell division in order to insert the transgene into the host cell genome. Thus, attempts to render beta-cell allografts less immunogenic have had to rely on stable transfection of surrogate cells. Similarly, this problem has precluded the development of conditionally immortalized human beta cells for clinical allotransplantation. In this report, we demonstrate that adult human islet beta cells can be transduced by a new three-plasmid integrating lentiviral vector with an efficiency of 62 ± 1.8 % at a multiplicity of infection (MOI) of 2.5 in vitro. This work makes genetic engineering of adult human pancreatic beta cells possible for the first time, allowing strategies to render beta-cell allografts non-immunogenic to be optimized and to creating conditionally immortalized human beta cells for clinical transplantation. [Diabetalogia (1998) 41: 736–739]Type of Medium: Electronic ResourceURL: