Search Results - (Author, Cooperation:L. Lackey)

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  1. 1
    Staff View
    Publication Date:
    2013-02-08
    Publisher:
    Nature Publishing Group (NPG)
    Print ISSN:
    0028-0836
    Electronic ISSN:
    1476-4687
    Topics:
    Biology
    Chemistry and Pharmacology
    Medicine
    Natural Sciences in General
    Physics
    Keywords:
    Base Sequence ; Biocatalysis ; Breast Neoplasms/*enzymology/*genetics/pathology ; Cell Death ; Cell Line, Tumor ; Cytidine Deaminase/genetics/*metabolism ; DNA Damage/genetics ; DNA Fragmentation ; DNA, Neoplasm/genetics/metabolism ; Deamination ; Gene Expression Regulation, Enzymologic ; Gene Expression Regulation, Neoplastic ; Histones/metabolism ; Humans ; *Mutagenesis/genetics ; Phenotype ; *Point Mutation/genetics ; Tumor Suppressor Protein p53/genetics/metabolism ; Up-Regulation ; Uracil/metabolism
    Published by:
    http://www.nature.com/

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  2. 2
    Staff View
    ISSN:
    1476-5535
    Keywords:
    Keywords: TCE; toluene; jet fuel; bioluminescence; lux; co-metabolism; Pseudomonas putida
    Source:
    Springer Online Journal Archives 1860-2000
    Topics:
    Biology
    Process Engineering, Biotechnology, Nutrition Technology
    Notes:
    A tod-lux transcriptional fusion bioluminescent reporter strain, Pseudomonas putida B2, was developed to permit on-line analysis of trichloroethylene (TCE) transformation by toluene dioxygenase (todC1C2BA) in Pseudomonas putida F1. Strain B2 was exposed to toluene in growing and resting cell bioluminescence assays. The growing cells showed a direct correlation between bioluminescence and toluene concentration, while resting cells showed reproducible bioluminescence with repeated toluene exposures. In addition, P. putida B2 was encapsulated in alginate beads and used in a packed bed flow-through differential volume reactor. The TCE feed into the differential volume reactor was constant at 20 mg L−1 and toluene was pulsed in square-wave perturbations at 10 mg L−1. The system showed a direct correlation between the expression of the tod operon (as monitored by light output) and the co-metabolism of TCE. Approximately 20% of the TCE and 50% of the toluene was removed at a flow rate of 0.4 ml min−1. This approach allowed the on-line monitoring of tod gene expression and its relation to TCE biotransformation.
    Type of Medium:
    Electronic Resource
    URL:
    http://dx.doi.org/10.1038/sj.jim.2900334

    Articles: DFG German National Licenses